We narrowed to 2,124 results for: PORA

1. pBABE_hygro_mRFP1_NRF2

   Plasmid

   #136579

   Purpose

   mammalian expression of mRFP1-tagged human NRF2

   Depositor

   Kevin Janes

   Article

   Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

   Insert

   NRF2 (NFE2L2 Human)
   

   Use

   Retroviral

   Tags

   N-mRFP1-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. DD-Cas9 with filler sequence and Venus (EDCPV)

   Plasmid

   #90085

   Purpose

   This plasmid contains destabilized Cas9 and has Venus after P2A sequence. This vector also contains filler sequence which required to be remove for cloning of desired sgRNA

   Depositor

   Raffaella Sordella

   Article

   Senturk et al Nat Commun. 2017 Feb 22;8:14370. doi: 10

   Insert

   Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Destabilized Domain and Flag

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSLIK_hygro_BirA_NRF2

   Plasmid

   #136539

   Purpose

   Lentiviral expression vector for an inducible BirA-NRF2

   Depositor

   Kevin Janes

   Article

   Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

   Insert

   BirA-NRF2 (NFE2L2 Human)
   

   Use

   Lentiviral; Destination vector for gateway cloning

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLNCX2-mCherry-CHMP4B

   Plasmid

   #116923

   Purpose

   expresses mCherry-CHMP4B in mammalian cells

   Depositor

   Sanford Simon

   Article

   Bleck et al Proc Natl Acad Sci U S A. 2014 Aug 19;11

   Insert

   CHMP4B (CHMP4B Human)
   

   Use

   Retroviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. DD-Cas9 with filler sequence and Cre-ERT2 (EDCICE)

   Plasmid

   #90086

   Purpose

   This plasmid contains destabilized Cas9 and has Cre-ERT2 after IRES sequence. This vector also contains filler sequence which required to be removed for cloning of desired sgRNA

   Depositor

   Raffaella Sordella

   Article

   Senturk et al Nat Commun. 2017 Feb 22;8:14370. doi: 10

   Insert

   Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Destabilized Domain and Flag

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1 iCasper T2A HO1

   Plasmid

   #64278

   Purpose

   Expresses infrared fluorescent executioner caspase reporter (iCasper) plus human heme oxygenase 1

   Depositor

   Xiaokun Shu

   Article

   To et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

   Insert

   Infrared fluorescent executioner caspase reporter (iCasper) T2A heme oxygenase-1: (HMOX1 Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pXL002-ishRNA-beta-catenin-1

   Plasmid

   #36297

   Depositor

   Sean Palecek

   Article

   Lian et al Proc Natl Acad Sci U S A. 2012 May 29.

   Insert

   shRNA of beta-catenin-1 (CTNNB1 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   IRES-RFP-Puro

   Expression

   Mammalian

   Mutation

   Promoter

   TetO-H1

   Available since

   Aug. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pAAV-CAG-DIO-ChroME2s-ST-P2A-H2B-mRuby3

   Plasmid

   #170163

   Purpose

   Cation channelrhodopsin ChroME2s targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

   Depositor

   Hillel Adesnik

   Article

   Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

   Insert

   ChroME2s-P2A-H2B-mRuby3
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR-FUSN-mCh-Cry2olig

   Plasmid

   #101224

   Purpose

   FUS(1-214) fused to mCh-Cry2olig

   Depositor

   Clifford Brangwynne

   Article

   Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

   Insert

   FUS(aa1-214) (FUS Human)
   

   Use

   Lentiviral

   Tags

   mCherry-Cry2olig

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Feb. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCK306

    Plasmid

    #110544

    Purpose

    rhaBAD, a rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites. Allows precise & sustained gene expression in cyanobacteria.

    Depositor

    John Heap

    Article

    Kelly et al ACS Synth Biol. 2018 Apr 2. doi: 10.1021

    Inserts

    PrhaBAD
    yfp
    rhaS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    N/A, PrhaBAD, and kanR promoter (upstream of kanR…

    Available since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pVax1-hVEGF165

    Plasmid

    #74466

    Purpose

    expression of hVEGF165 in mammalian cells

    Depositor

    Loree Heller

    Article

    Marshall WG et al Gene Ther. 2010 Mar;17(3):419-23. doi: 1

    Insert

    vascular endothelial growth factor 165 (VEGFA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLM-vexGFP-Oct4

    Plasmid

    #22240

    Purpose

    2nd generation lentiviral vector

    Depositor

    Michel Sadelain

    Article

    Papapetrou et al Proc Natl Acad Sci U S A. 2009 Aug 4. 10

    Insert

    vexGFP_2A_OCT4 (POU5F1 synthetic fluorescent protein, Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. H2B-GFP-Aurora B WT

    Plasmid

    #184042

    Purpose

    Expresses H2B-GFP fused to Aurora B WT

    Depositor

    Lienhard Schmitz

    Article

    Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

    Insert

    AURKB (AURKB Human)
    

    Use

    Tags

    EGFP and H2B

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAGGS 3xFLAG Mbnl2

    Plasmid

    #217032

    Purpose

    Overexpression of Mbnl2 in mammalian cells

    Depositor

    Chaolin Zhang

    Article

    Weyn-Vanhentenryck et al Nat Commun. 2018 Jun 6;9(1):2189. doi: 1

    Insert

    Mbnl2 (Mbnl2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. Sec61β-Flag-APEX2-SOPP3

    Plasmid

    #229072

    Purpose

    Expresse APEX2-SOPP3 in ER in mammalian cell

    Depositor

    Weijun Pan

    Article

    Qu et al Cell Res. 2025 Feb;35(2):149-152. doi: 1

    Insert

    Express APEX2-SOPP3 in the ER (APEX2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHR EGFR-CD3ε-FusionRed

    Plasmid

    #188626

    Purpose

    Human EGFR labeled with CD3ε ITAMs for monitoring with CD3ε/ZtSH2 pYtag biosensor

    Depositor

    Jared Toettcher

    Article

    Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

    Insert

    EGFR (EGFR Human, Synthetic)
    

    Use

    Lentiviral

    Tags

    CD3ε-FusionRed

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. OMM-Flag-APEX2-SOPP3

    Plasmid

    #229073

    Purpose

    Expresses APEX2-SOPP3 targeted to outer mitochondrial membrane

    Depositor

    Weijun Pan

    Article

    Qu et al Cell Res. 2025 Feb;35(2):149-152. doi: 1

    Insert

    Express APEX2-SOPP3 in outer mitochondrial membrane (APEX2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAS_4xU6-PylT M15 (UUA) FLAG-Mma PylRS

    Plasmid

    #154774

    Purpose

    plasmid with 4xMma PylT (M15 mutant, UUA anticodon) cassette and Mma PylRS for ochre suppression in transient or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    Mma PylRS (pylS Methanosarcina mazei)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    in PylT: UUA anticodon and G10A, G14U, G16U, C20U…

    Promoter

    EF1

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMRXIP-GFP-CERT(PHD)

    Plasmid

    #221746

    Purpose

    Stable expression of GFP-CERT(PHD)in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

    Insert

    CERT (CERT1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    amino acids 1–116

    Promoter

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-R26-FLEX-mStr

    Plasmid

    #135619

    Purpose

    Donor vector for genomic targeting of a CAG-FLEX-mStrawberry cassette to the mouse Rosa26 locus

    Depositor

    Lin He

    Article

    Chen et al Cell Rep. 2019 Jun 25;27(13):3780-3789.e

    Insert

    CAG-FLEX-mStrawberry flanked by Rosa26 homology arms (Gt(ROSA)26Sor Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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21. pcDNA3.1(+)_Granulin 4-no linker

    Plasmid

    #176925

    Purpose

    express Granulin 4 without linker 5 in mammalian cells

    Depositor

    Thomas Kukar

    Article

    Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

    Insert

    Granulin 4 (GRN Human)
    

    Use

    Tags

    Twin-Strep and FLAG tags encoded after Signal Pep…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRSFDuet-v2 RsTAL At4CL

    Plasmid

    #126524

    Purpose

    expresses TAL (Rhodobacter sphaeroides) and 4CL (Arabidopsis thaliana paralog 1) for PYP chromophore biosynthesis

    Depositor

    Andrew Woolley

    Article

    Brechun et al Biochemistry. 2019 Jun 11;58(23):2682-26

    Inserts

    tyrosine ammonia lyase
    p-coumaroyl-CoA ligase (4CL1 Mustard Weed)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. tet_pLKO.1_puro_shNRF2 #2

    Plasmid

    #136585

    Purpose

    Expresses an inducible short hairpin targeting human NRF2 sequence

    Depositor

    Kevin Janes

    Article

    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

    Insert

    shNRF2v2 (NFE2L2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV-CAG-DIO-ChroME2f-ST-P2A-H2B-mRuby3

    Plasmid

    #171150

    Purpose

    Cation channelrhodopsin ChroME2f targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

    Depositor

    Hillel Adesnik

    Article

    Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

    Insert

    ChroME2f-P2A-H2B-mRuby3
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA™5/FRT/CMV_promoter-Cas9-E2A-mRFP

    Plasmid

    #164131

    Purpose

    Expresses SpCas9 and mRFP in mammalian cells (an insertion vector to generate Cas9-knockin cell lines)

    Depositor

    Hyongbum Kim

    Article

    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

    Insert

    SpCas9 (cas9 Synthetic)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLV-EF1A-LivePAR(Y107A)-Hygro

    Plasmid

    #176073

    Purpose

    EGFP fused to the C-terminus of a WWE domain containing the mutation Tyr107Ala & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    LivePAR (Y107A)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Point mutation to convert Try107 to Ala

    Promoter

    EF1A

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Lenti-pNFkB::Cre-mRuby3

    Plasmid

    #164137

    Purpose

    Lentiviral construct for building stable cell lines expressing Cre recombinase and mRuby3 in the presence of TNFa

    Depositor

    Hyongbum Kim

    Article

    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

    Insert

    NFkB promoter-Cre-T2A-mRuby3
    

    Use

    CRISPR, Cre/Lox, Lentiviral, and Synthetic Biolog…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    NFkB synthetic promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAS_4xG1 PylT FLAG-G1 PylRS Y125A

    Plasmid

    #154769

    Purpose

    plasmid with 4xG1 PylT cassette and G1 PylRS Y125A for amber suppression in transient or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    G1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Y125A

    Promoter

    EF1

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMRXIB-mRuby3-CERT(PHD)

    Plasmid

    #221762

    Purpose

    Stable expression of mRuby3-CERT(PHD) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

    Insert

    CERT (CERT1 Human)
    

    Use

    Retroviral

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    amino acids 1–116

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHR EGFR-SLP76-TagBFP

    Plasmid

    #188630

    Purpose

    Human EGFR labeled with SLP76 for monitoring with SLP76/VISH2 pYtag biosensor

    Depositor

    Jared Toettcher

    Article

    Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

    Insert

    EGFR (EGFR Human, Synthetic)
    

    Use

    Lentiviral

    Tags

    SLP76-TagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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