4,299 results

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pET28a-MSP1D1delltaH5

Plasmid

#71714

Purpose

Membrane scaffold protein (MSP), helix 5 deletion

Depositor

Gerhard Wagner

Article

Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

Insert

Apolipoprotein A1 (APOA1 Human)

Tags

6xHis and TEV site

Expression

Bacterial

Available Since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pCS2FA CO Tol2 TPase

Plasmid

#133032

Purpose

Expression vector for production of codon optimized Tol2 transposase

Depositor

Charles Nathan Hancock

Article

Mackey et al MicroPubl Biol. 2020 Jun 11;2020:10.1791

Insert

Codon optimized Tol2 Transposase

Use

In vitro expression

Mutation

Codon Optimized

Available Since

June 15, 2020

Availability

Academic Institutions and Nonprofits only

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4xM67 pTATA TK-Luc

Plasmid

#8688

Depositor

Jim Darnell

Article

Besser et al Mol Cell Biol 1999 Feb;19(2):1401-9.

Insert

4 repeated M67 sites

Use

Luciferase

Expression

Mammalian

Available Since

July 18, 2006

Availability

Academic Institutions and Nonprofits only

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DR274

Plasmid

#42250

Purpose

guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence; uses T7 promoter for in vitro transcription

Depositor

Keith Joung

Article

Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR; Zebrafish expression

Expression

Worm

Promoter

T7

Available Since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only

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pCAG-GFP

Plasmid

#11150

Purpose

Mammalian expression vector for expression of GFP (CAG promoter)

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

Has Service

Cloning Grade DNA

Insert

EGFP from Aequorea victoria

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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tet-pLKO-sgRNA-puro

Plasmid

#104321

Purpose

3rd generation lentiviral plasmid for inducible expression of sgRNA; derived from tet-pLKO-puro; puromycin selection. See manual for detailed protocols.

Depositor

Nathanael Gray

Article

Huang et al eLife. 2017 Sep 19;6. pii: e26693. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

H1/TO

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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Cyto-roGFP

Plasmid

#49435

Purpose

Expresses thiol redox-sensitive ratiometric sensor roGFP in cytosol of mammalian cells

Depositor

Paul Schumacker

Article

Waypa et al Circ Res. 2010 Feb 19;106(3):526-35. doi

Insert

roGFP2

Use

Adenoviral

Expression

Mammalian

Mutation

C48S, Q80R, S147C, and Q204C in Clontech's G…

Promoter

CMV

Available Since

Dec. 9, 2013

Availability

Academic Institutions and Nonprofits only

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pBABE-hygro CDK4 R24C

Plasmid

#11254

Depositor

Bob Weinberg

Article

Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

Insert

CDK4 (CDK4 Human)

Use

Retroviral

Expression

Mammalian

Mutation

R24C

Available Since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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pDEST-CMV-C-EGFP

Plasmid

#122844

Purpose

C-terminal EGFP tag. Gateway destination vector for mammalian expression.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Type

Empty backbone

Use

Gateway Cloning

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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Str-Golgin84_VSVG-SBP-EGFP

Plasmid

#65305

Purpose

synchronize trafficking of Golgin-84 from the Golgi apparatus (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Golgin-84 (GOLGA5 Human)

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

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TMPRSS2

Plasmid

#53887

Purpose

Expression of TMPRSS2

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Has Service

Cloning Grade DNA

Insert

TMPRSS2 (TMPRSS2 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pSIJ8

Plasmid

#68122

Purpose

Temperature sensitive plasmid for either lambda Red recombinase genes or flippase recombinase expression

Depositor

Alex Nielsen

Article

Jensen et al Sci Rep. 2015 Dec 8;5:17874. doi: 10.103

Inserts

flp recombinase
rhaS
rhaR

Expression

Bacterial

Promoter

rhamnose

Available Since

Dec. 8, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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p16sheLL

Plasmid

#37320

Depositor

John Schiller

Article

Buck et al PLoS Pathog. 2006 Jul;2(7):e69.

Insert

HPV16L1+L2

Expression

Mammalian

Promoter

CMV

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

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pMXs-3XFLAG-EGFP-OMP25

Plasmid

#83354

Purpose

For tagging mitochondria with Sigma FLAG epitopes

Depositor

David Sabatini

Article

Chen et al Cell. 2016 Aug 25;166(5):1324-1337.e11.

Insert

3XFLAG-EGFP-OMP25

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 26, 2016

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2 neo

Plasmid

#98292

Purpose

Variant of lentiCRISPRv2 that confers G418 resistance

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR and Lentiviral

Promoter

EF-1a

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-C2-NLRP3

Plasmid

#73955

Purpose

Express EGFP-tagged human NLRP3 in mammalian cells

Depositor

Christian Stehlik

Article

Khare et al Immunity. 2012 Mar 23;36(3):464-76. doi:

Insert

NLR family, pyrin domain containing 3 (NLRP3) (NLRP3 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV iE

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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pX330A-nBRD4/PITCh

Plasmid

#91794

Purpose

PITCh sgRNA, N-terminal BRD4 sgRNA, and Cas9 expressing plasmid for use with the dTAG knock-in system and BRD4

Depositor

James Bradner, Behnam Nabet

Article

Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

Insert

SpCas9

Use

CRISPR

Expression

Mammalian

Promoter

chicken β-actin promoter

Available Since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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pBB540

Plasmid

#27393

Depositor

Bernd Bukau

Article

de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

Insert

grpE, clpB

Expression

Bacterial

Available Since

April 26, 2012

Availability

Academic Institutions and Nonprofits only

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3XFlag-pA-Tn5-Fl

Plasmid

#124601

Purpose

Expresses protein A and Tn5 Transposase fusion protein in bacterial cells

Depositor

Steven Henikoff

Article

Kaya-Okur et al Nat Commun. 2019 Apr 29;10(1):1930. doi:

Insert

Protein A and hyperactive Tn5 transposase (Tnp) fusion protein

Tags

Protein A and Tn5 transposase fusion protein with…

Expression

Bacterial

Promoter

T7

Available Since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO5.sgRNA.EFS.tRFP

Plasmid

#57823

Purpose

3rd generation Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP, EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

sgRNA
EFS
tagRFP

Use

CRISPR and Lentiviral

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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