165,688 results

1. pENTR-NLS-actin-R62D

   Plasmid

   #118381

   Purpose

   Gateway compatible donor vector with nuclear localization signal (NLS) attached to actin-R62D.

   Depositor

   Maria Vartiainen

   Article

   Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

   Insert

   actin beta (ACTB Human)
   

   Use

   Donor-plasmid

   Mutation

   NLS and R62D

   Available Since

   Nov. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. [#GS24 -LV] Syn1-GFP-OMP25

   Plasmid

   #233566

   Purpose

   Lentiviral construct of mitochondrial localized GFP to be expressed in the donor cells under the Synapsin1 promotor

   Depositor

   Simon Grelet

   Article

   Hoover et al Nature. 2025 Jun 25. doi: 10.1038/s41586

   Insert

   eGFP (eGFP Aequorea victoria, Synthetic)
   

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Available Since

   June 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5/FRT/TO_Lifeact_HaloTag9

   Plasmid

   #175545

   Purpose

   Expression of HaloTag9 at the actin filaments of mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   Lifeact-HaloTag9
   

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. qTAG-C-V5-Puro

   Plasmid

   #207730

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a V5-2A-Puro cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. mCh-Rab7A

   Plasmid

   #61804

   Purpose

   Human Rab7A fluorescently tagged with mCh on the N-terminus for mammalian expression

   Depositor

   Gia Voeltz

   Article

   Rowland et al Cell. 2014 Nov 20;159(5):1027-41. doi: 1

   Insert

   Rab7A (RAB7A Human)
   

   Tags

   mCh

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available Since

   Feb. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. mEmerald-Fascin-N-10

   Plasmid

   #54095

   Purpose

   Localization: Actin Bundling Protein, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Insert

   Fascin (FSCN1 Human)
   

   Tags

   mEmerald

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 16, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

7. GFP-KASH2

   Plasmid

   #187017

   Purpose

   Inducible disruption of LINC Complex

   Depositor

   Jan Lammerding

   Article

   Earle et al Nat Mater. 2019 Dec 16. pii: 10.1038/s41

   Insert

   KASH2
   

   Tags

   eGFP

   Expression

   Mammalian

   Available Since

   Aug. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. MSCV-opEBNA2-IRES-GFP

   Plasmid

   #220354

   Purpose

   Expresses EBV latent gene EBNA2 in mammalian cells (EBNA2 coding sequence was optimised for expression and carries a C-terminal 3xHA-tag)

   Depositor

   Klaus Rajewsky

   Article

   Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d

   Insert

   opEBNA2 (EBNA-2 Epstein-Barr Virus (EBV) strain B95-8)
   

   Use

   Retroviral

   Tags

   3 x HA tag

   Expression

   Mammalian

   Mutation

   codon-optimised for expression in mammalian cells

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRS307

   Plasmid

   #51785

   Purpose

   an integrating (YIp) vector with a LYS2 selectable marker

   Depositor

   David Stillman

   Article

   Eriksson et al Biotechniques. 2004 Feb;36(2):212-3.

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   June 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTol1-UAS:CoChR-tdTomato

    Plasmid

    #124233

    Purpose

    Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin CoChR fused to the red fluorescent protein tdTomato

    Depositor

    Isaac Bianco

    Article

    Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

    Insert

    CoChR-tdTomato
    

    Use

    Zebrafish expression

    Tags

    tdTomato

    Promoter

    14xUAS-E1b

    Available Since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLinker-BirA-3xHA-HXGPRT-loxP

    Plasmid

    #86668

    Purpose

    for generation of BirA tagged strains using CRISPR tagging technology

    Depositor

    David Sibley

    Insert

    Linker-BirA-3xHA-HXGPRT 3'UTR
    

    Expression

    Bacterial

    Available Since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBroad-T7RNAP-chlorR

    Plasmid

    #199992

    Purpose

    T7 RNAP circuit variant T15 on ultra broad host range vector (RSF1010 and pAMB1 origins) chlorR

    Depositor

    Farren Isaacs

    Article

    Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

    Insert

    T7RNAP Circuit
    

    Expression

    Bacterial

    Mutation

    copFnull

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-nuclear-EKAR4

    Plasmid

    #174439

    Purpose

    Nuclear-targeted EKAR4.

    Depositor

    Jin Zhang

    Article

    Keyes et al eLife. 2020 May 26;9. pii: 57410. doi: 1

    Insert

    nuclear-EKAR4
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET mCherry LIC cloning vector (u-mCherry)

    Plasmid

    #29769

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    mCherry

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHJ12: CadC-VHH-Caffeine (No linker)

    Plasmid

    #108244

    Purpose

    Membrane bound transcription activator CadC fusing VHH-Caffeine. Upon ligand binding, CadC-VHH-Caffeine can be dimerized and activate down stream reporter gene sfGFP expression

    Depositor

    Jerome Bonnet

    Article

    Chang et al ACS Synth Biol. 2017 Oct 30. doi: 10.102

    Insert

    Membrane bound transcription activator CadC fusing with VHH-Caffeine antibody without external linker
    

    Use

    Synthetic Biology

    Promoter

    pLacO1

    Available Since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. p663-UBC-V5-BRSK1_IDG-K

    Plasmid

    #135270

    Purpose

    Gateway destination clone of BRSK1 (human) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    V5-BRSK1 (BRSK1 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMRE-Tn7-165

    Plasmid

    #118577

    Purpose

    miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mScarlet
    

    Use

    Minitn7 delivery vector

    Expression

    Bacterial

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA HA DUSP6

    Plasmid

    #236430

    Purpose

    transient overexpression of DUSP6 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Duan et al Cell Rep. 2021 Oct 19;37(3):109870. doi:

    Insert

    DUSP6 (DUSP6 Human)
    

    Expression

    Mammalian

    Available Since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. MEK1C222

    Plasmid

    #164638

    Purpose

    Bacterial expression plasmid for His6-MEK1C222

    Depositor

    Ben Davis

    Article

    Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

    Insert

    mitogen-activated protein kinase kinase 1 (MAP2K1 Human)
    

    Tags

    His6-tag

    Expression

    Bacterial

    Mutation

    G(-19)F/S222C/C277S/C376S

    Promoter

    T7

    Available Since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBAD-HisD-KRaION1

    Plasmid

    #177799

    Purpose

    Bacterial expression of green fluorescent potassium indicator KRaION1

    Depositor

    Edward Boyden

    Article

    Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

    Insert

    KRaION1
    

    Expression

    Bacterial

    Promoter

    araB

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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