12,913 results

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pmNFL

Plasmid

#83127

Purpose

Mouse neurofilament protein L cDNA mammalian expression construct

Depositor

Anthony Brown

Article

Yan et al Cell Motil Cytoskeleton. 2007 Apr;64(4):

Insert

mouse NFL (Nefl Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pEN244 - CTCF-AID[71-114]-eGFP-FRT-Blast-FRT targeting construct

Plasmid

#92140

Purpose

Targeting vector to introduce an AID-eGFP cassette at the mouse CTCF locus using BLASTICIDIN selection. Auxin-inducible degron system.

Depositor

Benoit Bruneau

Article

Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

Insert

AID[71-114]-eGFP

Use

Mouse Targeting

Expression

Mammalian

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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pGL6

Plasmid

#48746

Purpose

Expression of luciferase driven by mPer2 promoter region (bases -1128 to +2129 with respect to transcription start site at +1)

Depositor

Joseph Takahashi

Article

Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10

Insert

mPer2 promoter/enhancer region (-1128 to +2129)

Use

Luciferase

Available Since

Dec. 19, 2013

Availability

Academic Institutions and Nonprofits only
Anti-Histone H3.3 [N158/28R]

Plasmid

#182106

Purpose

Mammalian Expression Plasmid of anti-Histone H3.3 (Rat). Derived from hybridoma N158/28.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Histone H3.3 (Rattus norvegicus) recombinant mouse monoclonal antibody (H3f3b Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-tetO-Oct4

Plasmid

#19766

Depositor

Konrad Hochedlinger

Article

Stadtfeld et al Cell Stem Cell. 2008 Mar 6. 2(3):230-40.

Insert

Oct4 (Pou5f1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pH-nCas9-PPE

Plasmid

#140447

Purpose

For plant prime editing in rice plants or monocotyledons protoplasts

Depositor

Caixia Gao

Article

Lin et al Nat Biotechnol. 2020 May;38(5):582-585.

Insert

nCas9(H840A)-M-MLV

Use

CRISPR

Expression

Plant

Mutation

H840A for Cas9; D200N, T306K, W313F, T330P and L…

Promoter

maize Ubiquitin-1, OsU3

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-Ran-Q69L-mRFP1-polyA

Plasmid

#104560

Purpose

Express Ran Q69L mutant in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

Ran (Ran Mouse)

Tags

mRFP1

Expression

Mammalian

Mutation

Q69L

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3 V5 mDispatched WT 2x HA

Plasmid

#126410

Purpose

Expression of V5 Dispatched WT HA in mammalian cell lines.

Depositor

Stacey Ogden

Article

Stewart et al Elife. 2018 Jan 23;7. pii: 31678. doi: 1

Insert

m-Dispatched1 (Disp1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

V5 iserted between A106 and A107

Available Since

June 14, 2019

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA_AIO-GZV-SynZiFTR_mNgn2 donor

Plasmid

#229789

Purpose

Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one grazoprevir-inducible expression of mouse neurogenin 2

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

mNgn2; ZF10-NS3-p65 (Neurog2 Mouse)

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF10 and CAG

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pFlox_N-Nipbl_Neo_FKBPF36V_mScarlet

Plasmid

#163883

Purpose

Acute Depletion NIPBL-mScarlet

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

Nipbl (Nipbl Mouse)

Expression

Mammalian

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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rAAV-syn::FLEX-rev::PSAMQ79G,Q139G:5HT3HC-IRES-GFP

Plasmid

#32475

Purpose

Cre-dependent expression of PSAM-5HT3 HC (Q79G,Q139G) neuronal activator, with IRES-EGFP marker

Depositor

Scott Sternson

Article

Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

Insert

PSAMQ79G,Q139G-5HT3HC

Use

AAV and Cre/Lox; Adeno-associated virus, flex swi…

Tags

IRES-EGFP

Promoter

Synapsin

Available Since

Dec. 12, 2011

Availability

Academic Institutions and Nonprofits only

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pUNO mouse CD274 + 3'UTR WT full length

Plasmid

#107012

Purpose

Expresses murine PD-L1 with the 3'UTR

Depositor

Julian Downward

Article

Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

Insert

CD274 (Cd274 Mouse)

Expression

Mammalian

Available Since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

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pSPTg.T2FXK (#54)

Plasmid

#35963

Depositor

Thomas Sato

Article

Schlaeger et al Proc Natl Acad Sci U S A. 1997 Apr 1;94(

Inserts

Tie2 promoter
Tie2 enhancer

Available Since

July 9, 2013

Availability

Academic Institutions and Nonprofits only

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CAG::PSAML141F,Y115F:5HT3HC-IRES-GFP

Plasmid

#32476

Purpose

Expresses PSAM-5HT3 HC (L141F,Y115F) neuronal activator, with IRES-EGFP marker

Depositor

Scott Sternson

Article

Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

Insert

PSAM Y115F,L141F-5HT3 HC

Tags

IRES-GFP

Expression

Mammalian

Promoter

CAG

Available Since

April 10, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV5 HA FoxO1

Plasmid

#14936

Depositor

Joan Massague

Article

Seoane et al Cell. 2004 Apr 16. 117(2):211-23.

Insert

FoxO1 (Foxo1 Mouse)

Tags

HA

Expression

Mammalian

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
VGlut2 scFv [N29/29]

Plasmid

#182085

Purpose

Mammalian Expression of VGlut2 scFv. Derived from hybridoma N29/29.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting VGlut2 (Rattus norvegicus) (Slc17a6 Mouse)

Tags

HA, His

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pclbw-opa1(isoform 1)-myc

Plasmid

#62845

Purpose

Mammalian Expression of myc-tagged Opa1 isoform1

Depositor

David Chan

Article

Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi

Insert

mmOPA1isoform1-myc (Opa1 Mouse)

Use

Retroviral

Tags

9xMyc

Expression

Mammalian

Promoter

cmv-bactin

Available Since

Jan. 11, 2016

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag p38 delta

Plasmid

#20785

Depositor

Roger Davis

Article

Enslen et al EMBO J. 2000 Mar 15. 19(6):1301-11.

Insert

pDCNA3 Flag p38 delta (Mapk13 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

April 14, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_NTS1.0

Plasmid

#180006

Purpose

Expresses the neurotensin sensor GRAB_NTS1.0 in neurons

Depositor

Yulong Li

Insert

neurotensin sensor GRAB_NTS1.0

Use

AAV

Promoter

hSyn

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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mP58.dJ1-pCDNA3

Plasmid

#21884

Depositor

David Ron

Article

Oyadomari et al Cell. 2006 Aug 25. 126(4):727-39.

Insert

P58 (Dnajc3 Mouse)

Expression

Mammalian

Mutation

DnaJ domain deleted from C-term

Available Since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pGL4.26-Ccnd2PromoterRegion2(0.5kb)

Plasmid

#228059

Purpose

0.5 kb from mouse Ccnd2 promoter in front of firefly luciferase reporter

Depositor

Kenneth Chen

Article

Fraire et al bioRxiv [Preprint]. 2024 Apr 30:2024.04.

Insert

Ccnd2 promoter (Ccnd2 Mouse)

Use

Luciferase

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL4.26-Igf2Promoter

Plasmid

#228060

Purpose

0.9 kb from mouse Igf2 promoter in front of firefly luciferase reporter

Depositor

Kenneth Chen

Article

Fraire et al bioRxiv [Preprint]. 2024 Apr 30:2024.04.

Insert

Igf2 promoter (Igf2 Mouse)

Use

Luciferase

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pGL3-Camk2a

Plasmid

#186818

Purpose

Fluorescent reporter for Camk2a expression

Depositor

Hung-Chun Chang

Article

Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

Insert

Camk2a promoter (Camk2a Mouse)

Use

Luciferase

Promoter

Camk2a promoter

Available Since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-Camk2b

Plasmid

#186819

Purpose

Fluorescent reporter for Camk2b expression

Depositor

Hung-Chun Chang

Article

Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

Insert

Camk2b promoter (Camk2b Mouse)

Use

Luciferase

Promoter

Camk2b promoter

Available Since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-Camk2d

Plasmid

#186820

Purpose

Fluorescent reporter for Camk2d expression

Depositor

Hung-Chun Chang

Article

Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

Insert

Camk2d promoter (Camk2d Mouse)

Use

Luciferase

Promoter

Camk2d promoter

Available Since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGl3-Camk2g

Plasmid

#186821

Purpose

Fluorescent reporter for Camk2g expression

Depositor

Hung-Chun Chang

Article

Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

Insert

Camk2g promoter (Camk2g Mouse)

Use

Luciferase

Promoter

Camk2g promoter

Available Since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Psicheck Cdk2ap1Exon1

Plasmid

#178028

Purpose

expression vector to test translation efficiency by luciferase reporter.

Depositor

Lin He

Article

Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

Insert

Cdk2ap1 (Cdk2ap1 Mouse)

Use

Luciferase; Nonviral

Tags

Luciferase

Expression

Mammalian

Promoter

Cloned in

Available Since

Jan. 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
psiCHECK-2-Ywhab-3'UTR

Plasmid

#61782

Purpose

Luciferase reporter containing the 3'UTR of mouse Ywhab

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Ywhab 3'UTR

Use

Luciferase

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Map3k1-3'UTR

Plasmid

#61776

Purpose

Luciferase reporter containing the 3'UTR of mouse Map3k1

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Map3k1 3'UTR

Use

Luciferase

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Fhod1-3'UTR

Plasmid

#61773

Purpose

Luciferase reporter containing the 3'UTR of mouse Fhod1

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Fhod1 3'UTR

Use

Luciferase

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Mapk12-3'UTR

Plasmid

#61783

Purpose

Luciferase reporter containing the 3'UTR of mouse Mapk12

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Mapk12 3'UTR

Use

Luciferase

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pLEX307-mFzd7

Plasmid

#102869

Purpose

Expresses mouse Fzd7 in mammalian cells

Depositor

Henry Ho

Article

Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

Insert

Frizzled 7 (Fzd7 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1A

Available Since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
mCardinal-H1-10

Plasmid

#56161

Purpose

Localization: Nucleus/Histones, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

H1 (H1f0 Mouse)

Tags

mCardinal

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pMXs-Oct3/4-IP

Plasmid

#15918

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

POU domain, class 5, transcription factor 1 (Pou5f1 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available Since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pH2B-iRFP670-N1

Plasmid

#197227

Purpose

Expresses the fusion protein of H2B-iRFP670 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

H2B-iRFP670

Expression

Mammalian

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-Trim46

Plasmid

#176402

Purpose

TRIM46 tagged with GFP

Depositor

Casper Hoogenraad, Thomas Schwarz

Article

van Beuningen et al Neuron. 2015 Dec 16;88(6):1208-1226. doi

Insert

trim46 (Trim46 Mouse)

Tags

GFP

Expression

Mammalian

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28b_RanWT

Plasmid

#186270

Purpose

Expression vector of His-RanQ69L in E.coli

Depositor

Masahito Ikawa

Article

Mori et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

Insert

GTP-binding nuclear protein Ran (Ran Mouse)

Tags

His tagged

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBetaActin-TUBB2a-mEos3.2-mEos3.2-mEos3.2

Plasmid

#191338

Purpose

Expresses triple fuorescently labeled beta tubulin to allow photoconversion of microtubule patches from green to red fluorescent with UV light in slice cultures

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

TUBB2a-mEos3.2-mEos3.2-mEos3.2 (Tubb2a Mouse)

Expression

Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDHa_mDDX5_FL_wt

Plasmid

#88869

Purpose

constitutive expression of Ha-DDX5 in mammalian cells

Depositor

Dan Littman

Article

Huang et al Nature. 2015 Dec 24;528(7583):517-22. do

Insert

DDX5 (Ddx5 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.CMV.SV40.THBS4-HA.SV40(polyA)

Plasmid

#155193

Purpose

Expresses murine Thrombospondin-4 (THBS4) protein with HA-tag at C-terminus

Depositor

Kevin Park

Article

Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

Insert

Thrombospondin-4 (Thbs4 Mouse)

Use

AAV

Tags

HA-tag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag-ECFP-betaPIXa

Plasmid

#15235

Depositor

Rick Horwitz

Article

Mayhew et al J Proteome Res. 2006 Sep . 5(9):2417-23.

Insert

beta-PIXa (Arhgef7 Mouse)

Tags

ECFP and Flag

Expression

Mammalian

Available Since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only
pCI-neo:Beta2meGFP

Plasmid

#45096

Depositor

Henry Lester

Article

Srinivasan et al J Gen Physiol. 2011 Jan;137(1):59-79.

Insert

nAChR beta2 (Chrnb2 Mouse)

Expression

Mammalian

Mutation

There are strain differences between C57BJ/6 and …

Promoter

CMV

Available Since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CMV-FLEX-SaCas9-U6-sgOprk1

Plasmid

#159903

Purpose

Mutagenesis of Oprk1

Depositor

Larry Zweifel

Insert

Oprk1 (Oprk1 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-Vim

Plasmid

#46315

Depositor

Xiangli Yang

Article

Lian et al J Biol Chem. 2009 Oct 30;284(44):30518-2

Insert

Vimentin (Vim Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pTA-Luc SIRT1 promoter (-202)

Plasmid

#10971

Depositor

Toren Finkel

Article

Nemoto et al Science. 2004 Dec 17. 306(5704):2105-8.

Insert

SIRT1 promoter (Sirt1 Mouse)

Use

Luciferase

Expression

Mammalian

Available Since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
GFAP scFv [N206A/8]

Plasmid

#199426

Purpose

Mammalian expression of GFAP scFv. Derived from hybridoma N206A/8.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targetingGFAP (Human) (GFAP Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pmCherry-Cep290

Plasmid

#27380

Depositor

Joseph Gleeson

Article

Valente et al Nat Genet. 2006 Jun . 38(6):623-5.

Insert

Cep290 (Cep290 Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

please see depositor comments below

Available Since

June 1, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4/HisMax-Synectin

Plasmid

#35943

Depositor

Arie Horowitz

Article

Naccache et al Proc Natl Acad Sci U S A. 2006 Aug 22;10

Insert

Synectin (Gipc1 Mouse)

Tags

6xHIS and Xpress

Expression

Mammalian

Mutation

K111R

Promoter

CMV

Available Since

June 22, 2012

Availability

Academic Institutions and Nonprofits only
pBS mNotch1-IC (DM#270)

Plasmid

#15079

Depositor

Douglas Melton

Article

Murtaugh et al Proc Natl Acad Sci U S A. 2003 Dec 9. 10

Insert

Notch1-IC (Notch1 Mouse)

Expression

Bacterial

Mutation

K1750A

Available Since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pmiRFP720-Actin-C-18

Plasmid

#197221

Purpose

Expresses the fusion protein of miRFP720-Actin-C-18 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

miRFP720-Actin-C-18

Expression

Mammalian

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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12,913 results