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Plasmid#182898PurposeMammalian expression of TH rat mAb HC geneDepositorInsertanti-TH (human) light chain (TH Rat)
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationNoPromoterCMVAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
TH mAb_BCN53.1.1D10_LC_Rat Ig Kappa
Plasmid#182899PurposeMammalian expression of TH rat mAb LC geneDepositorInsertanti-TH (human) light chain (TH Rat)
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationNoPromoterCMVAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAD100_hIRG1_4-461_pvp008
Plasmid#124875PurposeExpresses hCAD Arg273His mutant in E.coliDepositorInsertcis-aconitate decarboxylase (ACOD1 Human)
UseTagsStrepTag and TEV siteExpressionBacterialMutationmutated Arg273 to His, deleted amino acids 1-3 an…PromoterT7Available sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYPQ151D10A-x3.7
Plasmid#124309PurposeGateway entry vector with pcoCas9D10A-x3.7 (Plant codon optimized)DepositorInsertpcoCas9D10A-x3.7
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAtCas9-D10A_1
Plasmid#91159PurposeCas9 only expression, Cas9 Type: AtCas9_D10A (nickase), Plant Selection: 2x35S:hpt IIDepositorInsertAtCas9_D10A (nickase)
UseCRISPRTagsExpressionPlantMutationD10APromoterAvailable sinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
Syntaxin-4 d10
Plasmid#44188DepositorInsertSyntaxin-4
UseTags2xMyc and HisExpressionMammalianMutationdeleted first 10 amino acidsPromoterAvailable sinceJuly 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEM-T ZfHoxd10a
Plasmid#27543DepositorInsertHoxd10a (hoxd10a Zebrafish)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDD104 (Peft-3::Cre)
Plasmid#47551PurposeCre recombinase expression plasmid for removal of floxed selectable markers in the C. elegans germlineDepositorInsertCre Recombinase
UseCre/LoxTagsExpressionWormMutationPromotereef-1A.1 (eft-3)Available sinceSept. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDe-CAS9-D10A
Plasmid#61477Purposebased on pDe-CAS9, encodes Cas9 D10A nickase variantDepositorInsertCas9-D10A
UseCRISPRTagsExpressionPlantMutationcatalytic Asp10 changed to AlaPromoterPcUbi4-2Available sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
lentiCas9n(D10A)-Blast
Plasmid#63593PurposeExpresses human codon-optimized S. pyogenes Cas9n (D10A nickase) protein and blasticidin resistance from EFS promoter. Lentiviral backbone.DepositorInsertsCas9
Blasticidin resistance
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationD10A, nickase mutantPromoterEFS-NSAvailable sinceApril 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST1374-GCN4-D10A
Plasmid#113022PurposeExpresses GCN4-D10A in mammalian cellsDepositorInsertGCN4-D10A (GCN4 Budding Yeast, S. pyogenes)
UseTagsExpressionMammalianMutationD10APromoterCMVAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX335A_hCas9(D10A)_IRF8gRNA1
Plasmid#89719PurposeKnockout IRF8 in human cellsDepositorInsertIRF8 gRNA1 (IRF8 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS415_pGal-nCas9(D10A)
Plasmid#79618PurposeExpresses nCas9(D10A) in yeast cellsDepositorInsertSpCas9
UseTagsExpressionYeastMutationD10A for nickase Cas9PromoterpGal1Available sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
Rad51–Cas9(D10A)
Plasmid#125567PurposeExpresses the Rad51–Cas9(D10A) fusion construct in mammalian cells under CMV promoterDepositorInsertRad51–Cas9(D10A) (RAD51 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX335A_hCas9(D10A)_IRF8gRNA2
Plasmid#89720PurposeKnockout IRF8 in human cellsDepositorInsertIRF8 gRNA2 (IRF8 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEBB COMMD10 Flag
Plasmid#74902PurposeExpress COMMD10 in mammalian cellsDepositorInsertCOMMD10 (COMMD10 Human)
UseTagsFlagExpressionMammalianMutationPromoterEF-1alpha promoterAvailable sinceJune 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
TMED10-bio-His
Plasmid#51852PurposeExpresses full-length Transmembrane emp24 domain-containing protein precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertTMED10 (TMED10 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
CD109-bio-His
Plasmid#51862PurposeExpresses full-length CD109 antigen precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertCD109 (CD109 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-PIK3CB-D1067Y
Plasmid#116551PurposeLentiviral expression of PIK3CB D1067YDepositorInsertPIK3CB (PIK3CB Human)
UseLentiviralTagsExpressionMutationD1067YPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pST1374-D10A-GCN4
Plasmid#113025PurposeExpresses D10A-GCN4 in mammalian cellsDepositorInsertD10A-GCN4 (GCN4 Budding Yeast, S. pyogenes)
UseTagsExpressionMammalianMutationD10APromoterCMVAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only