161,252 results

1. pHAGE2_EF1a_TetOn_IRES_mCherry

   Plasmid

   #204155

   Purpose

   Lentivirus backbone expressing rtTA linked to mCherry

   Depositor

   Jesse Bloom

   Article

   Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

   Insert

   Tet-On 3G
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC57-LbNOX

   Plasmid

   #75285

   Purpose

   This plasmid contains human codon optimized sequence of LbNOX which is a water-forming NADH oxidase that can be used to increase NAD+/NADH ratio in mammalian cells.

   Depositor

   Vamsi Mootha

   Article

   Titov et al Science. 2016 Apr 8;352(6282):231-5. doi

   Insert

   LbNOX
   

   Use

   Tags

   C-terminal FLAG tag with linker

   Expression

   Mutation

   Human codon optimized

   Promoter

   Available since

   May 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pB-GLUT4-7myc-GFP

   Plasmid

   #52872

   Purpose

   detection and measurement of GLUT4 translocation

   Depositor

   Jonathan Bogan

   Article

   Bogan et al Mol Cell Biol. 2001 Jul;21(14):4785-806.

   Insert

   GLUT4 (Slc2a4 with human c-terminus, Rat)
   

   Use

   Retroviral

   Tags

   7x-myc (internal) and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-DIO-BACE-HA

   Plasmid

   #232353

   Purpose

   Beta-Secretase to be expressed (1:1) with either ATLAS protein.

   Depositor

   Don Arnold

   Article

   Rivera et al Nat Methods. 2025 May;22(5):1101-1111. d

   Insert

   BACE (BACE1 Human)
   

   Use

   AAV

   Tags

   HA

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   May 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-GFP

   Plasmid

   #11150

   Purpose

   Mammalian expression vector for expression of GFP (CAG promoter)

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

   Has Service

   Cloning Grade DNA

   Insert

   EGFP from Aequorea victoria
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCE-mp53DD

   Plasmid

   #41856

   Purpose

   Non-integrating (episomal) expression of mouse p53DD - p53 carboxy-terminal dominant-negative fragment

   Depositor

   Shinya Yamanaka

   Article

   Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

   Insert

   Trp53 (Trp53 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Δ40-903

   Promoter

   CAG

   Available since

   July 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pATetO 6XHis-Taq DNA Ligase

   Plasmid

   #178798

   Purpose

   Inducible expression of Taq DNA Ligase in E. coli

   Depositor

   Andrew Ellington

   Article

   Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

   Insert

   Taq DNA Ligase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. mCherry-Lysosomes-20

   Plasmid

   #55073

   Purpose

   Localization: Lysosome Membrane, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   LAMP1
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. kiCAP-AAV-MyoAAV2A

   Plasmid

   #224440

   Purpose

   Rep/Cap plasmid for the production of MyoAAV 2A, a muscle-tropic AAV capsid in mice.

   Depositor

   Pardis Sabeti

   Article

   Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

   Insert

   AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
   

   Use

   AAV

   Tags

   Expression

   Mutation

   RGDQTTL insert between amino acids 588 and 589 of…

   Promoter

   p41

   Available since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-(empty)-TAG

    Plasmid

    #138209

    Purpose

    To facilitate restriction cloning of an RNA of interest with a TGT recognition element in the 3' region

    Depositor

    Neal Devaraj

    Article

    Busby et al Methods Enzymol. 2020;641:373-399. doi:

    Type

    Empty backbone

    Use

    T7 transcription

    Tags

    TAG sequence

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Promoter, T7 Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-Neo-Bam APC

    Plasmid

    #16507

    Depositor

    Bert Vogelstein

    Article

    Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

    Insert

    APC (APC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMSCV_IRES_GFP_CD16a

    Plasmid

    #196189

    Purpose

    Retroviral expression plasmid for generating stable FCGR3A (CD16a)-expressing cell lines.

    Depositor

    Ana Ruiz-Saenz

    Article

    Vincken et al STAR Protoc. 2023 Apr 17;4(2):102224. do

    Insert

    CD16a (FCGR3A Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    changed Phenylalanine 158 to Valine

    Promoter

    LTR

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-CAG-PinkyCaMP

    Plasmid

    #232860

    Purpose

    An AAV vector expressing the mScarlet based calcium indicator PinkyCaMP under CAG promoter

    Depositor

    Olivia Masseck

    Article

    Fink et al bioRxiv [Preprint]. 2025 Jan 11:2024.12.

    Insert

    PinkyCaMP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRK5_mCherry_P2AT2A_GFP-nb-KS

    Plasmid

    #238233

    Purpose

    For overexpression of mCherry_P2AT2A_GFP-nb-KS

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    mCherry_P2AT2A_GFP-nb-KS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSANG10-3F-BG4

    Plasmid

    #55756

    Purpose

    Expresses BG4 scFv anti-G-quadruplex antibody in bacteria

    Depositor

    Shankar Balasubramanian

    Article

    Biffi et al Nat Chem. 2013 Mar;5(3):182-6. doi: 10.1

    Insert

    BG4
    

    Use

    Tags

    6xHis, pelB leader, and tri-FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. EGFR-GFP

    Plasmid

    #32751

    Purpose

    Mammalian expression of epidermal growth factor receptor (EGFR) fused to EGFP

    Depositor

    Alexander Sorkin

    Article

    Carter et al J Biol Chem. 1998 Dec 25;273(52):35000-7

    Insert

    EGFR (EGFR Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. XLone-GFP

    Plasmid

    #96930

    Purpose

    Tunable and temporal expression control of GFP

    Depositor

    Xiaojun Lian

    Article

    Randolph et al Sci Rep. 2017 May 8;7(1):1549. doi: 10.1

    Insert

    Green Fluorescent Protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3GS

    Available since

    Aug. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pZac2.1-gfaABC1D-PinkyCaMP

    Plasmid

    #232859

    Purpose

    An AAV vector expressing the mScarlet based calcium indicator PinkyCaMP under gfaABC1D promoter

    Depositor

    Olivia Masseck

    Article

    Fink et al bioRxiv [Preprint]. 2025 Jan 11:2024.12.

    Insert

    PinkyCaMP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    gfaABC1D

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAVS1-Neo-M2rtTA

    Plasmid

    #60843

    Purpose

    AAVS1 donor vector for genomic targeting

    Depositor

    Rudolf Jaenisch

    Article

    DeKelver et al Genome Res. 2010 Aug;20(8):1133-42. doi:

    Inserts

    M2rtTA
    Neo
    

    Use

    Targeting donor

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pmCherry-Gal3

    Plasmid

    #85662

    Purpose

    Fluorescent reporter for lysosomal damage

    Depositor

    Hemmo Meyer

    Article

    Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

    Insert

    LGALS3 (LGALS3 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLenti-CMV-MCS-GFP-SV-puro

    Plasmid

    #73582

    Purpose

    Third generation lentiviral vector expressing GFP and puromycin

    Depositor

    Paul Odgren

    Article

    Witwicka et al PLoS One. 2015 Jun 4;10(6):e0128275. doi

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    GFP tag

    Expression

    Mutation

    Promoter

    Available since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMK381 (AAVS1 CMV-OsTIR1F74G)

    Plasmid

    #140536

    Purpose

    AAVS1 CMV-OsTIR1(F74G)

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    CMV-OsTIR1(F74G)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. HER2 WT

    Plasmid

    #16257

    Depositor

    Mien-Chie Hung

    Article

    Li et al Cancer Cell. 2004 Nov . 6(5):459-69.

    Insert

    HER2 (ERBB2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. MSCV-IRES-GFP

    Plasmid

    #20672

    Purpose

    Depositor

    Tannishtha Reya

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    IRES EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. VCP(wt)-EGFP

    Plasmid

    #23971

    Depositor

    Nico Dantuma

    Article

    Tresse et al Autophagy. 2010 Feb 22. 6(2):.

    Insert

    Valosin containing protein (VCP Human)
    

    Use

    Tags

    6xHis, EGFP, and HA

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    Feb. 26, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET-NLS-Cas9-6xHis

    Plasmid

    #62934

    Purpose

    Expression of NLS-Cas9-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    NLS-Cas9-6xHis
    

    Use

    Tags

    6xHis tag and NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. LentiV2-hU6-evopreQ1

    Plasmid

    #210189

    Purpose

    pegRNA/ngRNA library cloning

    Depositor

    Yin Shen

    Article

    Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. AAV.CBA.eGFP.2A.wtTau

    Plasmid

    #140424

    Purpose

    AAV.CBA.eGFP.2A.wtTau propagation reporter WT-2N4R-hTau

    Depositor

    Bradley Hyman

    Article

    Wegmann et al Sci Adv. 2019 Jun 26;5(6):eaaw6404. doi:

    Insert

    eGFP-2A-Tau (MAPT Synthetic, Human)
    

    Use

    AAV

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    CBA

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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29. pJRH-1346 U6-B2M sgRNA Gag-pol v2

    Plasmid

    #201917

    Purpose

    Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Inserts

    Gag-pol
    B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and Human U6

    Available since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSOUP

    Plasmid

    #165419

    Purpose

    The pSOUP helper plasmid needed for replication of all pGreen (Hellens et al., 2000) based vectors in Agrobacterium

    Depositor

    Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing

    Article

    Hayta et al Curr Protoc. 2021 Mar;1(3):e58. doi: 10.

    Insert

    Rep A
    

    Use

    Helper plasmid required for replication for all p…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge