165,307 results

1. hROSA26_Hygro_hPGK1_mScarlet-I_hCdt1(30-120)

   Plasmid

   #191391

   Purpose

   Donor for targeted integration of hCdt1(30-120) probe to the hROSA26 safe harbor locus

   Depositor

   Yannick Doyon

   Insert

   hCdt1(30-120)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   mScarlet-I

   Expression

   Mammalian

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHS0806 His14-bdSUMO-AwaIscB

   Plasmid

   #176539

   Purpose

   Bacterial expression of His14-bdSUMO-AwaIscB

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

   Insert

   AwaIscB
   

   Use

   CRISPR

   Tags

   14xHis-bdSUMO tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3 Flag Fli1

   Plasmid

   #66976

   Purpose

   Expression plasmid for mouse Fli1; adds Flag tag to the N-terminus of protein

   Depositor

   Christopher Vakoc

   Article

   Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

   Insert

   Fli1 (Fli1 Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

4. NEK7_HUMAN_D0

   Plasmid

   #79735

   Purpose

   This plasmid encodes the kinase domain of NEK7. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   John Chodera, Nicholas Levinson, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   NEK7 (NEK7 Human)
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

5. TFORF2043

   Plasmid

   #142316

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ARID3A (ARID3A Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBMN-dGBP1-TagBFP

   Plasmid

   #80087

   Purpose

   MMLV retroviral vector for expression of dGBP1-TagBFP with t-hcRed as an infection/transfection marker

   Depositor

   Connie Cepko

   Article

   Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

   Insert

   dGBP1-TagBFP
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Feb. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT7-EGFP-C1-HsNXF-1_K

   Plasmid

   #146740

   Purpose

   Mammalian Expression of HsNxf-1

   Depositor

   Elisa Izaurralde

   Article

   Gatfield et al EMBO J. 2003 Aug 1. 22(15):3960-70.

   Insert

   HsNxf-1 (NXF1 Human)
   

   Expression

   Mammalian

   Mutation

   S144G relative to NM_006362.4

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1 T21R-vhhGFP4-T2A-mCherry

   Plasmid

   #225587

   Purpose

   Mammalian expression vector for TRIM21 RING-vhhGFP4 anti-GFP degrader with a self-cleaving T2A-mCherry fluorescent expression reporter

   Depositor

   William McEwan

   Article

   Benn et al Science. 2024 Aug 30;385(6712):1009-1016

   Insert

   T21R-vhhGFP4-T2A-mCherry
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJO672 (pRS415 Gal1-Ufd4-flag)

   Plasmid

   #159590

   Purpose

   expresses Ufd4-flag in yeast cells

   Depositor

   Alexander Varshavsky

   Insert

   Ufd4
   

   Expression

   Yeast

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBR HPV31 9E

    Plasmid

    #221009

    Purpose

    Human papillomavirus 31 isolate 9E, complete genome cloned in pBR322

    Depositor

    Alison McBride

    Insert

    Human papillomavirus type 31 genome 9E isolate
    

    Use

    Cloning vector

    Available Since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGM

    Plasmid

    #71250

    Purpose

    luciferase reporter with human GM-CSF promoter -627 to + 28

    Depositor

    Peter Cockerill

    Article

    Cockerill et al Proc Natl Acad Sci U S A. 1999 Dec 21;96

    Insert

    GM-CSF promoter -627 to +28 fragment (CSF2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Promoter

    GM-CSF

    Available Since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV-CMV-hXRCC1-mCherry-Hygro

    Plasmid

    #176532

    Purpose

    mCherry fused to the C-terminus of XRCC1 & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Wilk et al Sci Rep. 2020 Jan 20;10(1):651. doi: 10.

    Insert

    X-Ray Repair Cross Complementing 1 (XRCC1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNeonGreen-C1-Nir2-LNS2

    Plasmid

    #220086

    Purpose

    C-terminal PA binding domain of Nir2

    Depositor

    Gerry Hammond

    Article

    Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

    Insert

    PITPNM1 (PITPNM1 Human)
    

    Tags

    NeonGreen-GGSGGM

    Expression

    Mammalian

    Mutation

    amino acids 896-1216

    Promoter

    CMV

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEM705 Gsi (Cys)

    Plasmid

    #109373

    Purpose

    GsX chimera with Gαi C-terminus (Pertussis toxin sensitive)

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    Gsi chimera
    

    Tags

    None

    Expression

    Mammalian

    Mutation

    Cys at position 391 (Pertussis Toxin Sensitive)

    Promoter

    CAG

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. CCR5_gRNA_pX459

    Plasmid

    #192268

    Purpose

    gRNA expression for CRISPR Genome Editing of CCR5

    Depositor

    Shantanu Chowdhury

    Article

    Sharma et al Cell Rep. 2021 May 18;35(7):109154. doi:

    Insert

    CCR5 guide RNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. TERTpWT_Gluc_CCR5

    Plasmid

    #192270

    Purpose

    Donor template for insertion of hTERT promoter driven Gaussia luciferase reporter

    Depositor

    Shantanu Chowdhury

    Article

    Sharma et al Cell Rep. 2021 May 18;35(7):109154. doi:

    Insert

    Promoter Reporter Insert within donor template
    

    Use

    CRISPR

    Promoter

    TERT promoter driving expression of gaussia

    Available Since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. RC(WW)-LgBit

    Plasmid

    #208931

    Purpose

    Recognition domain for in vitro MAPK-activity sensor

    Depositor

    Attila Reményi

    Article

    Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

    Insert

    WW domain of PIN1 (PIN1 Human)
    

    Tags

    HISx6, LgBit, and MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJT302 Ec03 Lenti pEF-attB LSR GFP

    Plasmid

    #184945

    Purpose

    Lentiviral delivery of recombinase landing pad

    Depositor

    Lacra Bintu, Michael Bassik, Ami Bhatt, Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Ec03 Lenti pEF1a-attB LSR GFP
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCas9-CAT

    Plasmid

    #80323

    Purpose

    Encodes Cas9 and chloramphenicol acetyltransferase (CAT)

    Depositor

    Sebastian Lourido

    Article

    Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

    Inserts

    Cas9
    Chloramphenicol acetyltransferase
    

    Use

    CRISPR

    Tags

    3X FLAG and NLS

    Promoter

    TgTUB1

    Available Since

    Sept. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDG330

    Plasmid

    #100898

    Purpose

    SpCas9 with a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.

    Depositor

    Paul Thomas

    Article

    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

    Inserts

    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    

    Use

    CRISPR and Mouse Targeting

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CBh and U6

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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21. pcDNA5FRT/TO-UBXD4-Strep-HA

    Plasmid

    #113480

    Purpose

    Expression of human UBXD4 with C-terminal strep-HA tag

    Depositor

    Hemmo Meyer

    Article

    Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

    Insert

    UBXD4 (UBXN2A Human)
    

    Tags

    2xstrep-HA

    Expression

    Mammalian

    Mutation

    wildtype without stop codon

    Promoter

    CMV

    Available Since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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22. LRP6 deltaN-pCS2-VSVG

    Plasmid

    #27281

    Depositor

    Xi He

    Article

    Tamai et al Mol Cell. 2004 Jan 16. 13(1):149-56.

    Insert

    low density lipoprotein receptor-related protein 6 (LRP6 Human)
    

    Use

    Xenopus injection

    Tags

    VSVG

    Mutation

    contains amino acid residues 1370–1613 (extracell…

    Available Since

    Jan. 26, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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23. pJT303 Ec04 Lenti pEF-attB LSR GFP

    Plasmid

    #184946

    Purpose

    Lentiviral delivery of recombinase landing pad

    Depositor

    Lacra Bintu, Michael Bassik, Ami Bhatt, Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Ec04 Lenti pEF1a-attB LSR GFP
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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24. pSLIK MEK5-DD-mRFP1 neo

    Plasmid

    #47548

    Purpose

    Lentiviral expression vector for an inducible mRFP1-tagged constitutively active MEK5 mutant

    Depositor

    Kevin Janes

    Article

    Jensen et al Cell Host Microbe. 2013 Jan 16;13(1):67-

    Insert

    MEK5-DD-mRFP1 (MAP2K5 Human)
    

    Use

    Lentiviral

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    S311D, T315D

    Available Since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

25. pCW-Cas9

    Plasmid

    #50661

    Purpose

    Doxycycline-inducible lentiviral expression of SpCas9

    Depositor

    David Sabatini, Eric Lander

    Article

    Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

    Insert

    humanized S. pyogenes Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    Tet ON

    Available Since

    Jan. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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26. pUdO2c

    Plasmid

    #210231

    Purpose

    medium-copy plasmid for bacterial expression

    Depositor

    François-Xavier Campbell-Valois

    Article

    Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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27. pMJA089

    Plasmid

    #128518

    Purpose

    Expresses human Lysozyme in Pichia pastoris

    Depositor

    Marcos Alcocer

    Article

    Johnson et al J Mol Biol. 2005 Sep 30;352(4):823-36. d

    Insert

    Artificial gene for human lysozyme (LYZ Human)
    

    Expression

    Yeast

    Promoter

    AOX1

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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28. pEM705 Gs13

    Plasmid

    #109359

    Purpose

    GsX chimera with Gα13 C-terminus

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    Gs13 chimera
    

    Tags

    None

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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29. pYeDP60-hATP13A2-E343A

    Plasmid

    #171378

    Purpose

    A modified pYEDP60 plasmid containing a yeast codon-optimized version of human ATP13A2 variant 2 E343A mutant, followed by a thrombin cleavage site and a C-term BAD tag. DOI: 10.21769/BioProtoc.3888

    Depositor

    Peter Vangheluwe

    Article

    van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:

    Insert

    human ATP13A2 variant 2 wild-type
    

    Tags

    BAD tag

    Expression

    Yeast

    Mutation

    E343A

    Promoter

    URA3

    Available Since

    Sept. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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30. pTriEx-mCherry-LOV2 V416I

    Plasmid

    #81045

    Purpose

    mammalian expression of LOV V416I

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    LOV V416I
    

    Tags

    mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    V416I

    Available Since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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