6,471 results

1. FKBP_SCF2_LD6_NTEV

   Plasmid

   #58878

   Purpose

   MESA split-TEV protease chain with Rapamycin FKBP ectodomain, 2 aa scaffold, 6 aa linker domain, N-terminal TEV protease

   Depositor

   Joshua Leonard

   Article

   Daringer et al ACS Synth Biol. 2014 Mar 11.

   Insert

   MESA split protease chain with FKBP rapamycin-binding ectodomain and N terminal fragment of TEV protease
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSyn-DIO-Gluc-RMA-IRES-EGFP

   Plasmid

   #189630

   Purpose

   Expresses Gluc-RMA and EGFP in the presence of Cre recombinase. Double-floxed Gluc-RMA and EGFP for monitoring Cre-expressing neuronal populations.

   Depositor

   Jerzy Szablowski

   Article

   Lee et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

   Inserts

   Gaussia luciferase fused to Fc
   IRES-EGFP
   

   Use

   AAV, Cre/Lox, and Luciferase

   Tags

   Gluc and IgG1 Fc

   Expression

   Mammalian

   Promoter

   IRES and hSyn

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CaMP0.4-FHS-paCaMKII-WPRE3

   Plasmid

   #165429

   Purpose

   Expresses photoactivatable CaMKII in mammalian cells to induce synaptic plasticity.

   Depositor

   Hideji Murakoshi

   Article

   Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

   Insert

   FHS-paCaMKII
   

   Use

   AAV

   Tags

   FHS(Flag, HisX6, Strep) tag

   Expression

   Mammalian

   Promoter

   CaMKII0.4

   Available Since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hGfap-R-CaMP1.07-WPRE-SV40

   Plasmid

   #164140

   Purpose

   AAV vector to express the red Ca2+ indicator R-CaMP1.07 in astrocytes.

   Depositor

   Hirokazu Hirai, Hajime Hirase

   Article

   Oe et al Nat Commun. 2020 Jan 24;11(1):471. doi:

   Insert

   R-CaMP1.07
   

   Use

   AAV

   Promoter

   hGFAP

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-DIO-hMECP2-cycle3GFP

   Plasmid

   #163706

   Purpose

   pAAV plasmid for Cre-dependent expression of human MECP2 fused with cycle3 GFP under Syn promoter

   Depositor

   Michisuke Yuzaki

   Article

   Ito-Ishida et al J Neurosci. 2020 Nov 4;40(45):8746-8766.

   Insert

   hMECP2-cycle3GFP (MECP2 Aequorea victoria, Human)
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CaMKIIa-eArch 3.0-EYFP

   Plasmid

   #35516

   Purpose

   AAV expression of CaMKIIa-driven Arch 3.0 for optical inhibition.

   Depositor

   Karl Deisseroth

   Article

   Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

   Insert

   Arch3.0-EYFP
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Enhanced trafficking signal and ER export signal …

   Promoter

   CaMKIIa

   Available Since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV hSyn FLEx-OFF hM3Dq-mCherry

   Plasmid

   #161578

   Purpose

   To express hM3Dq in cells not expressing Cre.

   Depositor

   Kevin Beier, Robert Malenka

   Article

   Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

   Insert

   hM3Dq-mCherry
   

   Use

   AAV

   Mutation

   NA

   Promoter

   hSyn

   Available Since

   Nov. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV syn Barcode1

   Plasmid

   #229062

   Purpose

   Expression mapping

   Depositor

   Scott Sternson

   Insert

   Syn Barcode1
   

   Use

   AAV

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-FLEX-SaCas9-U6-sgSlc18a2

   Plasmid

   #124849

   Purpose

   Mutagenesis of Slc18a2

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Slc18a2 (Slc18a2 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Available Since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. 132_pAAV-ProB2-CatCh-GFP-WPRE

    Plasmid

    #125922

    Purpose

    Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

    Depositor

    Botond Roska

    Article

    Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

    Insert

    CatCh-GFP
    

    Use

    AAV

    Promoter

    ProB2

    Available Since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-Ef1a-DIO C1V1 (t/t)-TS-mCherry

    Plasmid

    #35498

    Purpose

    AAV expression of Ef1a-driven, cre-dependent, chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.

    Depositor

    Karl Deisseroth

    Article

    Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

    Insert

    ChR1-VChR1 Chimera
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    E122T and E162T

    Promoter

    Ef1a

    Available Since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV gfaABC1D NES-jRCaMP1a

    Plasmid

    #171120

    Purpose

    Astrocyte AAV-mediated expression of jRCaMP1a, a red fluorescent calcium sensor protein.

    Depositor

    Thomas Oertner

    Article

    Lohr et al Front Cell Neurosci. 2021 Jun 11;15:6901

    Insert

    jRCaMP1a
    

    Use

    AAV

    Tags

    His Tag, T7 Tag, XpressTag

    Expression

    Mammalian

    Promoter

    gfaABC1D

    Available Since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV.synP.DIO.EGFP.WPRE.hGH

    Plasmid

    #100043

    Purpose

    An AAV vector that expresses EGFP in a Cre-dependent manner (DIO, double floxed inverted open reading frame).

    Depositor

    Ian Wickersham

    Has Service

    AAV9

    Insert

    EGFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    synapsin-1

    Available Since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pACAGW-ChR2-Venus-AAV

    Plasmid

    #20071

    Purpose

    AAV-mediated expression of ChR2-Venus for neuronal excitation

    Depositor

    Karel Svoboda

    Article

    Leopoldo et al Nature. 2009 Jan 18.

    Has Service

    AAV1 and AAV9

    Insert

    Channelrhodopsin-2 (CHLRE_02g085257v5 Chlamydomonas reinhardtii)
    

    Use

    AAV

    Tags

    Venus

    Expression

    Mammalian

    Promoter

    CAGG

    Available Since

    Jan. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-hSyn-Flpo-3xFLAG

    Plasmid

    #173047

    Purpose

    AAV expression of Flpo-3xFLAG by hSyn1 promoter

    Depositor

    Kenji Mizuseki

    Article

    Kitanishi et al Commun Biol. 2022 Feb 21;5(1):149. doi:

    Insert

    Flpo
    

    Use

    AAV

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    hSyn1

    Available Since

    Feb. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST

    Plasmid

    #105448

    Purpose

    Cation channelrhodopsin ChrimsonR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites in a viral vector

    Depositor

    Hillel Adesnik

    Article

    Pegard et al Nat Commun. 2017 Oct 31;8(1):1228. doi:

    Has Service

    AAV9

    Insert

    ChrimsonR-mRuby2-ST
    

    Use

    AAV

    Tags

    mRuby2 and soma targeting motif

    Mutation

    K176R in Chrimson

    Promoter

    hSyn

    Available Since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV.CMV.SV40.THBS4-HA.SV40(polyA)

    Plasmid

    #155193

    Purpose

    Expresses murine Thrombospondin-4 (THBS4) protein with HA-tag at C-terminus

    Depositor

    Kevin Park

    Article

    Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

    Insert

    Thrombospondin-4 (Thbs4 Mouse)
    

    Use

    AAV

    Tags

    HA-tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-hSyn-JEDI-2P-Kv-WPRE

    Plasmid

    #179463

    Purpose

    Soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector for neuron -specific expression using the promoter hSyn

    Depositor

    Francois St-Pierre

    Article

    Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

    Has Service

    AAV9

    Insert

    JEDI-2P-Kv
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. AiP15285 - pAAV-AiE0170h_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN5285)

    Plasmid

    #230509

    Purpose

    AiE0170h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

    Depositor

    Allen Institute for Brain Science, Jonathan Ting

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    iCre(R297T)
    

    Use

    AAV

    Promoter

    minBG

    Available Since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV.Ple155-CI-mRuby2-W3SL_BbsI(GGA)

    Plasmid

    #231364

    Purpose

    Ple155-driven mRuby2, containing cassette for BbsI-based Golden Gate assembly (e.g. of sgRNA cassette(s)). Can be used as 'no guide' control.

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    mRuby2
    

    Use

    AAV

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. CD4-MKII-hLOV1-TEVcs(ENLYFQ/M)- Gal4

    Plasmid

    #163026

    Purpose

    FLiCRE TF for HEK293T expression

    Depositor

    Alice Ting

    Article

    Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

    Insert

    CD4-MKII-hLOV1-TEVcs-Gal4
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV-hSyn-Flex-Axon-EGFP

    Plasmid

    #135429

    Purpose

    Can be used to drive axon-enriched expression of EGFP in the presence of Cre recombinase.

    Depositor

    Rylan Larsen

    Has Service

    AAV1

    Insert

    axon-EGFP
    

    Use

    AAV and Cre/Lox

    Tags

    GAP43 palmitoylation domain

    Expression

    Mammalian

    Promoter

    hSyn1

    Available Since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pOTTC469 - pAAV EF1a DIO eNpHR3.0-iRFP

    Plasmid

    #47631

    Purpose

    An AAV vector that expresses engineered halorhodopsin 3.0 (fused to iRFP) in a Cre-dependent manner (DIO, double floxed inverted open reading frame).

    Depositor

    Brandon Harvey

    Article

    Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

    Insert

    eNpHR3.0
    

    Use

    AAV and Cre/Lox

    Tags

    iRFP

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV.CAG.iGluSnFR3.v857.SGZ

    Plasmid

    #178334

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857 in Stargazin (SGZ) backbone. iGluSnFR3.v857.SGZ

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    pAAV CAG iGluSnFR3 v857.SGZ
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    IgK-chain, Myc epi tag, and Stargazin and PDZ dom…

    Expression

    Mammalian

    Available Since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV-hSynapsin1-axon-GCaMP6s-P2A-mKate2

    Plasmid

    #112004

    Purpose

    For enriched expression of GCaMP6s in axons with cytosolic expression of mKate2

    Depositor

    Lin Tian

    Article

    Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

    Has Service

    AAV9

    Insert

    axon-GCaMP6s-P2A-mKate2
    

    Use

    AAV

    Tags

    GAP43 palmitoylation domain

    Expression

    Mammalian

    Promoter

    hSynapsin1

    Available Since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. AiP1924 - pAAV-AiE2543m-minBG-SYFP2-WPRE-BGHpA

    Plasmid

    #214583

    Purpose

    AiE2543m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

    Depositor

    Allen Institute for Brain Science, Bosiljka Tasic

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Promoter

    minBG

    Available Since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV-PTRE-tight-flex-hM3Dq-mCherry-WPRE-pA

    Plasmid

    #115161

    Purpose

    This plasmid is for use with neuronal cell-type selective activity tagging. The hM3Dq receptor expression requires both neural activity and Cre recombinase.

    Depositor

    William Wisden

    Article

    Harding et al Curr Biol. 2018 Jul 23;28(14):2263-2273.

    Inserts

    PTRE - tet activator responsive promoter
    flex sequence
    hM3dq-mCherry (inverted)
    flex sequence
    

    Use

    AAV

    Tags

    hM3Dq-mCherry

    Expression

    Mammalian

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Cbh_v5 AAV-saCBE_KKH C-terminal

    Plasmid

    #137184

    Purpose

    AAV genome: expresses the C-terminal of S. aureus v5 AAV-CBE, and U6-sgRNA (KKH variant).

    Depositor

    David Liu

    Article

    Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

    Insert

    v5 AAV-saCBE_KKH C-terminal
    

    Use

    AAV

    Mutation

    E782K;N968K;R1015H conferring recognition of NNNR…

    Promoter

    Cbh

    Available Since

    Jan. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-CMV-iRFP713(V256C)-P2A-EGFP

    Plasmid

    #198071

    Purpose

    The AAV vector for iRFP713(V256C) and EGFP

    Depositor

    Michiyuki Matsuda

    Insert

    iRFP713(V256C)
    

    Use

    AAV

    Tags

    P2A-EGFP

    Mutation

    V256C mutation

    Promoter

    CMV

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pOTTC589 - pAAV c-fos Nuc-eYFP

    Plasmid

    #59133

    Purpose

    An AAV vector that expresses nuclear localized eYFP under control of the c-fos promoter.

    Depositor

    Brandon Harvey

    Article

    Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

    Insert

    Nuclear-localized Enhanced Yellow Fluorescent Protein
    

    Use

    AAV

    Tags

    3xNLS

    Expression

    Mammalian

    Promoter

    mFos

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. AAV-FLEX-PKIalpha-IRES-nls-mRuby2

    Plasmid

    #63059

    Purpose

    Expresses PKIalpha and nuclear localized mRuby2 (linked with IRES) in a Cre-dependent manner in an AAV backbone

    Depositor

    Bernardo Sabatini

    Article

    Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

    Insert

    PKIalpha (Pkia Mouse, Synthetic)
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. 011LP-pAAV-TNT4-EmGFP-pA

    Plasmid

    #120280

    Purpose

    AAV vector for cardiomyocyte specific expression of Emerald Green Fluorescent Protein using cardiac troponin T promoter

    Depositor

    Xander Wehrens

    Article

    Ni et al Circ Res. 2019 Jan 18;124(2):256-262. do

    Insert

    EmGFP
    

    Use

    AAV

    Tags

    3XFlag and HA

    Promoter

    TNT Cardiac Troponin T promoter

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAAV-hSyn-GRAB_NTS1.0

    Plasmid

    #180006

    Purpose

    Expresses the neurotensin sensor GRAB_NTS1.0 in neurons

    Depositor

    Yulong Li

    Insert

    neurotensin sensor GRAB_NTS1.0
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAAV-DIO-TVA-V5-RG

    Plasmid

    #119743

    Purpose

    Expresses TVA, V5, and RG in a cre dependent manner.

    Depositor

    Marie Carlén

    Article

    Ahrlund-Richter et al Nat Neurosci. 2019 Apr;22(4):657-668. do

    Insert

    TVA, V5, Rabies Glycoprotein
    

    Use

    AAV and Cre/Lox

    Tags

    V5

    Available Since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAAV-CaMKIIa-ChRmine-eYFP-WPRE

    Plasmid

    #130988

    Purpose

    AAV vector to drive the expression of ChRmine-eYFP under the control of CamKIIa promoter

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Insert

    ChRmine
    

    Use

    AAV

    Tags

    eYFP

    Promoter

    CaMKIIa

    Available Since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. kiCAP-AAV-MyoAAV1C

    Plasmid

    #224438

    Purpose

    Rep/Cap plasmid for the production of MyoAAV 1C, a muscle-tropic AAV capsid in mice and macaques.

    Depositor

    Pardis Sabeti

    Article

    Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

    Insert

    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    

    Use

    AAV

    Mutation

    RGDLSTP insert between amino acids 588 and 589 of…

    Promoter

    p41

    Available Since

    Sept. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. AiP1969 - pAAV-AiE2563m-minBG-SYFP2-WPRE-BGHpA

    Plasmid

    #214597

    Purpose

    AiE2563m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

    Depositor

    Allen Institute for Brain Science, Bosiljka Tasic

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Promoter

    minBG

    Available Since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Circular 200,100 (RAB7A)

    Plasmid

    #170118

    Purpose

    AAV vector carrying a guide RNA targeting the human RAB7A mRNA

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

    Insert

    Circular 200,100 guide RNA (RAB7A Human)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Human U6

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAAV-AcGFP-progerin

    Plasmid

    #86947

    Purpose

    Construct used to synthesize progerin mRNA. Please note the plasmid contains LMNA gene with nucleotide C1824T mutation.

    Depositor

    Alex Hewitt

    Insert

    AcGFP-progerin (LMNA Human)
    

    Use

    AAV

    Tags

    AcGFP

    Expression

    Mammalian

    Mutation

    progerin sequence is LMNA gene with nucleotide C1…

    Promoter

    CMV and T7

    Available Since

    June 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAAV_EF1a-fDIO-PdCO-mScarlet-miniWPRE

    Plasmid

    #198515

    Purpose

    Expresses optimized PdCO in frame with mScarlet under control of eukaryotic Ef1a promoter after Flp-dependent recombination.

    Depositor

    Ofer Yizhar

    Article

    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

    Insert

    PdCO
    

    Use

    AAV and Cre/Lox

    Tags

    Rho1D4 and mScarlet

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge