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CROPseq-Guide-Zeo

Plasmid

#127173

Purpose

Entry plasmid for guide cloning

Depositor

Paul Blainey

Article

Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pCFD3.1-w-dU6:3gRNA

Plasmid

#123366

Purpose

gRNA expression plasmid for CRISPR mutagenesis in Drosophila (with mini-white transformation marker).

Depositor

Simon Bullock

Insert

U6:3-gRNA

Use

CRISPR

Expression

Insect

Available Since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

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pEG302 22aa SunTag VP64 nog

Plasmid

#120251

Purpose

CRISPR-Cas9 SunTag system to target VP64 to specific loci of interest (nog=no guide)

Depositor

Steven Jacobsen

Article

Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

Insert

NOS_NLS_GB1_NLS_linker_VP64_linker sfGFP_scFv_UBQ10_Insulator_ UBQ10_Ω dCas9_1xHA_3xNLS_linker_10xGCN4_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-DIO-COX4-dAPEX2

Plasmid

#117177

Purpose

Plasmid name in publication: pAAV-DIO-Matrix-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

Depositor

David Ginty

Article

Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

Insert

COX4-dAPEX2

Use

AAV and Cre/Lox

Expression

Mammalian

Mutation

W41F and A134P on soybean APX

Promoter

EF1a

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-FGFR3

Plasmid

#116742

Purpose

Lentiviral expression of FGFR3

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

FGFR3 (FGFR3 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLH-sgRNA-Sirius-4X(MS2-PP7)

Plasmid

#121941

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Article

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

sgRNA-Sirius-4X(MS2-PP7)

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Promoter

human U6 promoter

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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SynTagMA_pre

Plasmid

#119738

Purpose

Synaptic Tag for Mapping Activity

Depositor

Thomas Oertner

Article

Perez-Alvarez et al Nat Commun. 2020 May 18;11(1):2464. doi:

Insert

SynTagMA_pre

Use

AAV

Expression

Mammalian

Promoter

hsyn1

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAC1801_pmax-dCasRx

Plasmid

#118634

Purpose

Transient transfection; Expresses dCasRx; CAGGS promoter

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

dCasRx

Expression

Mammalian

Mutation

R239A/H244A/R858A/H863A

Promoter

CAGGS

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pscALPS- SIV-MAC251 vpx

Plasmid

#115810

Purpose

Encodes codon optimized 3xFLAG-VpxMAC251 and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVMAC251 Vpx

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pEJS1084_pTetR-P2A-BFP/BspMI flexible sgRNA

Plasmid

#117687

Purpose

U6 driven Spy sgRNA cloning vector where guide sequences are inserted between BspMI sites. This plasmid works with Addgene #108570 for subnuclear proteomic profiling via C-BERST method.

Depositor

Erik Sontheimer

Article

Gao et al Methods Enzymol. 2019;616:365-383. doi:

Inserts

Kanamycin cassette
TetR-P2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 and hPGK

Available Since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pBECKP-km

Plasmid

#117235

Purpose

A cytidine deaminase-mediated base-editing plasmid in Klebsiella Pneumoniae

Depositor

Quanjiang Ji

Article

Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

Insert

ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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NES-EGFP-PH-ARNO2G-I303Ex2

Plasmid

#116868

Purpose

PIP3 biosensor

Depositor

Gerry Hammond

Article

Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

Insert

CYTH2 (CYTH2 Human)

Tags

EGFP and X.leavis map2k1.L(32-44)

Expression

Mammalian

Mutation

amind acids 252-399, isoleucine 303 changed to gl…

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCWXPGR-pTF-betaCatenin

Plasmid

#114281

Purpose

TET-inducible expression of a constitutively active form of the BetaCatenin protein

Depositor

Patrick Salmon

Article

Boitard et al Cell Rep. 2015 Mar 3;10(8):1349-61. doi:

Insert

Catenin beta 1 (CTNNB1 Human)

Use

Lentiviral

Tags

FLAG

Mutation

deletion of AA 1 to 45

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pEJS654 All-in-One AAV-sgRNA-hNmeCas9

Plasmid

#112139

Purpose

Delivery of human-codon-optimized Cas9 from Neisseria meningitidis (NmeCas9) and its single-guide RNA in a single AAV vector for in vivo genome editing.

Depositor

Erik Sontheimer

Article

Ibraheim et al Genome Biol. 2018 Sep 19;19(1):137. doi:

Inserts

sgRNA scaffold
Human-codon-optimized NmeCas9

Use

AAV, CRISPR, and Mouse Targeting

Expression

Mammalian

Promoter

U1a and U6

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pAJM.657

Plasmid

#108525

Purpose

CymRAM + PCymRC-YFP Reporter. Cuma sensor for use in non-Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

CymRAM + PCymRC-YFP

Expression

Bacterial

Mutation

CymR: S110G A171V

Available Since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

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KRAB-dCas9

Plasmid

#112195

Purpose

KRAB fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

Depositor

David Segal

Article

O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

Insert

KRAB (ZNF10 Human)

Use

CRISPR

Tags

3XFLag-NLS-KRAB-dCas9-NLS

Expression

Mammalian

Promoter

CMV

Available Since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-xFNLS-P2A-Puro

Plasmid

#110872

Purpose

Lentiviral vector for constitutive expression of xFNLS in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

xFNLS(3.7)

Use

Lentiviral

Tags

3X FLAG

Mutation

D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

Promoter

EF1s

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynap.iGABASnFR.F102G

Plasmid

#112160

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR.F102G

Use

AAV

Mutation

F102G

Promoter

hSynapsin

Available Since

July 16, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSynapsin1-FLEx-axon-GCaMP6s

Plasmid

#112010

Purpose

For cre-dependent enriched expression of GCaMP6s in axons

Depositor

Lin Tian

Article

Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

Has Service

AAV5 and AAV9

Insert

axon-GCaMP6s

Use

AAV and Cre/Lox

Tags

GAP43 palmitoylation domain

Expression

Mammalian

Promoter

hSynapsin1

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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LLP457 pGK-dCas9-Suntag-BFP

Plasmid

#100957

Purpose

BPF version of LLP339, Puro is replaced by BFP driven by EF1a, dCas9 with SungTagx10 driven by pGK, with 3xHA tag

Depositor

Ryan Lister

Article

Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

Insert

dCas9, SunTag array (10xGCN4)

Use

CRISPR

Tags

3xHA and BFP

Expression

Mammalian

Mutation

D10A, H840A for dCas9

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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