We narrowed to 9,307 results for: try

1. pET21b-Caspase-3(V266E)

   Plasmid

   #90089

   Purpose

   Expresses caspase 3 V266E mutant in bacterial cells

   Depositor

   Clay Clark

   Article

   Pop et al Biochemistry. 2003 Oct 28;42(42):12311-2

   Insert

   Caspase 3 (CASP3 Human)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   V266E

   Promoter

   Available since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. HA-CYLD-WT

   Plasmid

   #15506

   Depositor

   Stephen Elledge

   Article

   Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

   Insert

   CYLD (CYLD Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 13, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. H2B-MiniR

   Plasmid

   #208328

   Purpose

   MiniR genetically encoded tag introduced after H2B; to be used with a MiniE probe peptide.

   Depositor

   Kimberly Beatty

   Article

   Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

   Insert

   H2B-MiniR
   

   Use

   Tags

   MiniR

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCK002 pME mCerulean

   Plasmid

   #195956

   Purpose

   Gateway pME fluorophore

   Depositor

   Christian Mosimann, Alexa Burger

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   mCerulean
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBad-mKalama1

   Plasmid

   #14892

   Purpose

   Bacterial expression of mKalama1 blue fluorescent protein

   Depositor

   Robert Campbell

   Article

   Ai et al Biochemistry. 2007 May 22;46(20):5904-10

   Insert

   mKalama1
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   The fluorescent protein was inserted between XhoI…

   Promoter

   Available since

   May 15, 2007

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA3.1/hChR2(H134R)-EYFP

   Plasmid

   #20940

   Purpose

   Mammalian expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activation

   Depositor

   Karl Deisseroth

   Article

   Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

   Insert

   channelrhodopsin-2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   H134R mutation in ChR2

   Promoter

   CMV

   Available since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pENTR221-TCF7

   Plasmid

   #79498

   Purpose

   MultiSite Gateway entry clones, second fragment (attL5, attL2) Human TCF7 CDS

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   TCF7 (TCF7 Human)
   

   Use

   Multisite gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   no Promoter

   Available since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Dimer cleaved

   Plasmid

   #198199

   Purpose

   FRET calibration: Dimer construct consisting of mTurquoise2 and mVenus with a 2A peptide linker for posttranslational linker cleavage

   Depositor

   Christian Wahl-Schott

   Article

   Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

   Insert

   mVenus-T2A-mTurquoise2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A206K both

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCK029 exorh:EGFP

   Plasmid

   #195980

   Purpose

   Expression construct

   Depositor

   Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   exorh:EGFP
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. human PNP non-tagged

    Plasmid

    #64199

    Purpose

    Expression of native human PNP (non-tagged)

    Depositor

    Vern Schramm

    Article

    Murkin et al Biochemistry. 2007 May 1;46(17):5038-49.

    Insert

    Purine Nucleoside Phosphorylase (PNP Human)
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHR-H13LTat-Thy1.2-P2A-GFP-T2A-Nef

    Plasmid

    #126553

    Purpose

    Replication incompetent HIV with H13L Tat and GFP-t2a-Thy1.2 reporter

    Depositor

    Jonathan Karn

    Article

    Dobrowolski et al MBio. 2019 Mar 26;10(2). pii: mBio.00337

    Insert

    HIV-1 vector pNL4-3
    

    Use

    Lentiviral

    Tags

    Thy1.2 and GFP

    Expression

    Mutation

    Promoter

    HIV LTR

    Available since

    July 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. scFv-IFP2.0N

    Plasmid

    #215746

    Purpose

    Expresses single chain Fragment variable (scFv) fused to N-terminal part of split IFP2.0

    Depositor

    Albert Jeltsch

    Article

    Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

    Insert

    IFP2.0N
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET29_LuxSit_i

    Plasmid

    #194299

    Purpose

    Bacterial expression of the de novo luciferase, LuxSit-i

    Depositor

    David Baker, Andy Yeh

    Article

    Yeh et al Nature. 2023 Feb;614(7949):774-780. doi:

    Insert

    LuxSit-i
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. GST-Keima-cb5

    Plasmid

    #137755

    Purpose

    Targets Keima to the endoplasmic reticulum. Used to monitor ER-phagy

    Depositor

    Carol Mercer

    Article

    Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

    Insert

    Keima-cb5
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Fus-BcLOV-mCh-SOS

    Plasmid

    #208275

    Purpose

    Expresses Fus-BcLOV-SOS for optogenetic activation of Ras/Erk signaling. Includes an mCherry tag.

    Depositor

    Lukasz Bugaj

    Article

    Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

    Insert

    Fus-BcLOV-mCh-SOS
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHR-H13LTat-CD8a/d2eGFP-IRES-Nef

    Plasmid

    #126552

    Purpose

    Replication incompetent HIV with H13L Tat and d2eGFP/CD8a reporter

    Depositor

    Jonathan Karn

    Article

    Dobrowolski et al MBio. 2019 Mar 26;10(2). pii: mBio.00337

    Insert

    HIV-1 vector pNL4-3
    

    Use

    Lentiviral

    Tags

    CD8a and GFP

    Expression

    Mutation

    Delta gag/pol

    Promoter

    HIV LTR

    Available since

    July 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pENTR-PpU6P-sgRNA-L3L2

    Plasmid

    #113740

    Purpose

    Gateway entry vector containing attL3 and attL2 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    PpU6P::sgRNA
    

    Use

    CRISPR; Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    P. patens U6 promoter

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pENTR-PpU6P-sgRNA-L1L4

    Plasmid

    #113736

    Purpose

    Gateway entry vector containing attL1 and attL4 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    PpU6P::sgRNA
    

    Use

    CRISPR; Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    P. patens U6 promoter

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 457 p3E-2A-EGFPpA

    Plasmid

    #195967

    Purpose

    Gateway p3E 2A fluorophore

    Depositor

    Christian Mosimann, Kristen Kwan

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    2A-EGFP
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMuLE_ENTR_CBF-tdTomato_L3-L2

    Plasmid

    #113708

    Purpose

    Single pathway reporter; notch promoter-driven expression of tdTomato; MultiSite Gateway entry vector.

    Depositor

    Manfred Ogris

    Article

    Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

    Insert

    CBF (notch dependent promoter)
    

    Use

    Mule gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCMV-Neo-Bam E2F4

    Plasmid

    #37966

    Depositor

    Jacqueline Lees

    Article

    Moberg et al Mol Cell Biol. 1996 Apr;16(4):1436-49.

    Insert

    E2F4 (E2F4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3.1 3xF-GAR1

    Plasmid

    #126873

    Purpose

    Expresses 3xFlag GAR1

    Depositor

    Kathleen Collins

    Article

    Egan et al Mol Cell Biol. 2010 Jun;30(11):2775-86.

    Insert

    GAR1 (GAR1 Human)
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pENTR5'-attP (pCM254)

    Plasmid

    #68311

    Purpose

    Gateway middle entry vector for full-length attP site for phiC31-mediated transgenesis.

    Depositor

    Christian Mosimann, Leonard Zon

    Article

    Mosimann et al Dev Dyn. 2013 Aug;242(8):949-63. doi: 10

    Insert

    attP
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. human MTAP

    Plasmid

    #64077

    Purpose

    Expression of human 5'-methylthioadenosine phosphorylase

    Depositor

    Vern Schramm

    Article

    Guan et al Biochemistry. 2011 Nov 29;50(47):10408-1

    Insert

    5'-methylthioadenosine phosphorylase (MTAP Synthetic, Human)
    

    Use

    Tags

    Histidine tag and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJCC_039 FnCas12a

    Plasmid

    #171666

    Purpose

    For bacterial expression of FnCas12a with a C-terminal sorting motif and His tag

    Depositor

    Jennifer Doudna

    Article

    Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

    Insert

    FnCas12a
    

    Use

    Tags

    6X His and sortase A sorting motif

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. His6_BcLOV4_mCherry_BamUK

    Plasmid

    #114596

    Purpose

    Encodes mCherry-tagged BcLOV4 for expression in bacterial cells

    Depositor

    Brian Chow

    Article

    Glantz et al Proc Natl Acad Sci U S A. 2018 Aug 14;11

    Insert

    BcLOV4
    

    Use

    Tags

    His6 and mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. GFP-DDK

    Plasmid

    #137764

    Purpose

    Control plasmid for immunoprecipitation of DDK/FLAG-tagged proteins. Also a good control for transfection efficiency.

    Depositor

    Carol Mercer

    Article

    Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

    Insert

    GFP
    

    Use

    Tags

    DDK

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3-PhoDer

    Plasmid

    #188473

    Purpose

    Expresses the optogenetic human Gasdermin D (PhoDer) in mammalian cells

    Depositor

    Gary Mo

    Article

    Santa Cruz Garcia et al Nat Commun. 2022 Jan 10;13(1):52. doi: 1

    Insert

    PhoDer (GSDMD Synthetic, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE

    Available since

    Nov. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. ND4.2-Right DdCBE-G1397-C- T1413I

    Plasmid

    #179685

    Purpose

    Expression of base editor in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

    Insert

    SOD2 MTS_3xHA_ND4.2 Right TALE_2aa_DddA G1397-C (T1413I)_1xUGI_SOD2 3'UTR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T1413I

    Promoter

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. MXS_CMV::PuroR-bGHpA

    Plasmid

    #62439

    Purpose

    MXS_chaining vector with CMV::PuroR-bGHpA

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    resistance cassette against Puromycin with CMV Promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pENTR1A-3XFlag-WT-MEFV

    Plasmid

    #167018

    Purpose

    Gateway entry plasmid with 3xflag-pyrin (Wild-type)

    Depositor

    Thomas Henry

    Article

    Chirita et al Methods Mol Biol. 2022;2523:179-195. doi

    Insert

    MEFV (MEFV Human)
    

    Use

    Gateway entry vector

    Tags

    3xFlag

    Expression

    Mutation

    Promoter

    Available since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pEN_TT 3xFLAG-XRCC6

    Plasmid

    #192302

    Purpose

    Gateway entry vector for an inducible 3xFLAG-XRCC6

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    XRCC6 (XRCC6 Human)
    

    Use

    Gateway entry vector

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. neurod1:CaMPARI

    Plasmid

    #173479

    Purpose

    CaMPARI is driven by -5kb neurod1 promoter.

    Depositor

    John Rawls

    Article

    Ye et al Cell Host Microbe. 2021 Feb 10;29(2):179

    Insert

    CaMPARI
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pYZ173

    Plasmid

    #98410

    Purpose

    Cas9-gRNA lys9 plasmid for lys9 deletion in S. pombe

    Depositor

    Jef Boeke

    Article

    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

    Insert

    gRNA targeting Sp.lys9 (SPBC3B8.03 Fission Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLenti-CaMKIIa-eNpHR-EYFP-WPRE

    Plasmid

    #20946

    Purpose

    3rd gen lentiviral expression of halorhodopsin 2.0 (eNpHR) fused to EYFP driven by CaMKIIa promoter for optogenetic inhibition

    Depositor

    Karl Deisseroth

    Article

    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

    Insert

    halorhodopsin
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa

    Available since

    May 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. 460 p3E-2A-mCherrypA

    Plasmid

    #195970

    Purpose

    Gateway p3E 2A fluorophore

    Depositor

    Christian Mosimann, Kristen Kwan

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    2A-mCherry
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pmKalama1-Nuc

    Plasmid

    #14894

    Purpose

    Mammalian expression of mKalama1 blue fluorescent protein with NLS

    Depositor

    Robert Campbell

    Article

    Ai et al Biochemistry. 2007 May 22;46(20):5904-10

    Insert

    pmKalama1-Nuc
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    I178T mismatch, replace EYFP of the vector pEYFP-…

    Promoter

    Available since

    May 15, 2007

    Availability

    Academic Institutions and Nonprofits only

38. pAtetO_6xHis-v5.9

    Plasmid

    #155259

    Purpose

    Plasmid for the aTc-inducible expression of the v5.9

    Depositor

    Andrew Ellington

    Article

    Milligan et al Biochemistry. 2018 Aug 7;57(31):4607-461

    Insert

    v5.9
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    tet PA

    Available since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pEF-ENTR-LacZ (710-1)

    Plasmid

    #17430

    Purpose

    Gateway entry vector with EF1-a promoter, V5-His C-term, Lac Z

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    LacZ
    

    Use

    Entry vector

    Tags

    His and V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pENTR-PpU6P-sgRNA-L5L2

    Plasmid

    #113738

    Purpose

    Gateway entry vector containing attL5 and attL2 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    PpU6P::sgRNA
    

    Use

    CRISPR; Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    P. patens U6 promoter

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge