We narrowed to 13,730 results for: TIM

1. HBT_pcoCASphi_version1

   Plasmid

   #197931

   Purpose

   Cloning vector with Arabidopsis codon optimized Casphi-2 protein, with SV40 NLS at the N-terminal and nucleoplasmin NLS at the C-terminal

   Depositor

   Steven Jacobsen

   Article

   Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

   Insert

   pcoCasphi-2
   

   Use

   CRISPR

   Tags

   FLAG tag and SV40 NLS and nucleoplasmin NLS

   Expression

   Plant

   Mutation

   Promoter

   35SPPDK promoter

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pPIDCB2

   Plasmid

   #169900

   Purpose

   Empty dox inducible vector with piggyBac, insulators, and a DTS. The constitutive mClover3 is fused to H2B to allow for visualization of the nucleus.

   Depositor

   Richard Schneider

   Article

   Chu et al Biol Open. 2020 Oct 6;9(10). pii: bio.05

   Type

   Empty backbone

   Use

   Avian expression

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Dox inducible

   Available since

   Dec. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Cry2(1-531)-mCh-BAXS184E

   Plasmid

   #117238

   Purpose

   Light activated recruitment of BAX in mammalian cells with Cry2/CIB optogenetic switch

   Depositor

   Robert Hughes

   Article

   Godwin et al J Biol Chem. 2019 Nov 8;294(45):16918-16

   Insert

   Cry2531-mCh-BAXS184E
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFLEX+mCyclin D1

   Plasmid

   #86793

   Purpose

   mammalian expression of cyclin D1

   Depositor

   Martine Roussel, Charles Sherr

   Article

   Matsushime et al Cell. 1991 May 17;65(4):701-13.

   Insert

   cyclin D1 (Ccnd1 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. BRAC/pcDNA3

   Plasmid

   #51967

   Purpose

   mammalian expression of BRAC, a BRET-based autofluorescent Ca2+ indicator

   Depositor

   Takeharu Nagai

   Article

   Saito et al PLoS One. 2010 Apr 1;5(4):e9935. doi: 10

   Insert

   BRAC
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 18, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pESC-URA::CYP71AV1/CPR

   Plasmid

   #20118

   Depositor

   Jay Keasling

   Article

   Ro et al Nature. 2006 Apr 13. 440(7086):940-3.

   Insert

   amorphadiene oxidase and p450 reductase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 2, 2009

   Availability

   Academic Institutions and Nonprofits only

7. pYZ173

   Plasmid

   #98410

   Purpose

   Cas9-gRNA lys9 plasmid for lys9 deletion in S. pombe

   Depositor

   Jef Boeke

   Article

   Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

   Insert

   gRNA targeting Sp.lys9 (SPBC3B8.03 Fission Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. 1/8-NORAD-(A)4x

   Plasmid

   #199209

   Purpose

   1/8 truncation of NORAD mRNA tagged with 4 copies of Riboglow (variant A)

   Depositor

   Esther Braselmann

   Article

   Sarfraz et al Nat Commun. 2023 Feb 16;14(1):867. doi:

   Insert

   1/8-NORAD
   

   Use

   Tags

   Riboglow RNA tag

   Expression

   Mammalian

   Mutation

   4 copies of Riboglow tag after stop codon

   Promoter

   CMV

   Available since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. GFP-C1-CAMKIIalpha-T286A

   Plasmid

   #21220

   Depositor

   Tobias Meyer

   Article

   Shen et al Science. 1999 Apr 2. 284(5411):162-6.

   Insert

   CAMKIIalpha-T286A (Camk2a Rat)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   T286A (Lab plasmid KS 0003).

   Promoter

   Available since

   July 29, 2009

   Availability

   Academic Institutions and Nonprofits only

10. pAD-sfGFP(Sp)-Pman

    Plasmid

    #133833

    Purpose

    Fluorescent protein sfGFP(Sp); under control of mannose inducible promoter.

    Depositor

    Oscar Kuipers

    Article

    Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57

    Insert

    sfGFP(Sp)
    

    Use

    E.coli/bacillus spp. shuttle vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pman

    Available since

    Nov. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. LAMP-ReNL_pcDNA3

    Plasmid

    #89532

    Purpose

    Red color variant of bright luminescent protein, fused to Lysosomal-associated membrane protein 1

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    ReNL
    

    Use

    Tags

    LAMP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. 1/2-NORAD-(A)4x

    Plasmid

    #199208

    Purpose

    1/2 truncation of NORAD mRNA tagged with 4 copies of Riboglow (variant A)

    Depositor

    Esther Braselmann

    Article

    Sarfraz et al Nat Commun. 2023 Feb 16;14(1):867. doi:

    Insert

    1/2-NORAD
    

    Use

    Tags

    Riboglow RNA tag

    Expression

    Mammalian

    Mutation

    4 copies of Riboglow tag after stop codon

    Promoter

    CMV

    Available since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEB2-TagRFP-T

    Plasmid

    #103998

    Purpose

    Plasmid encoding TagRFP-T

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    TagRFP-T
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to eqFP578 (R32G, K42R, K67R, …

    Promoter

    proC

    Available since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV-T7-SpRY-P2A-mScarlet (MNW002)

    Plasmid

    #174137

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R); C-terminal bi-partite NLS, 3x flag tag, P2A-mScarlet

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized SpRY with BPNLS-3xFLAG-P2A-mScarlet
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-mScarlet

    Expression

    Mammalian

    Mutation

    SpG=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317…

    Promoter

    CMV and T7

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. tetO-Myocd

    Plasmid

    #46033

    Depositor

    John Gearhart

    Article

    Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

    Insert

    Myocd (Myocd Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetO

    Available since

    July 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mMaroon-PAK-mMaroon

    Plasmid

    #84361

    Purpose

    FRET acceptor for Cdc42 donor, flanked with the far-red FP mMaroon

    Depositor

    Ryohei Yasuda

    Article

    Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    mMaroon-PAK-mMaroon
    

    Use

    Tags

    mMaroon flanking PAK domain on N and C terminus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. KA1153_pPBCAG-MerCreMer-IN

    Plasmid

    #124183

    Purpose

    piggyBac-based expression of the tamoxifen-inducible Cre

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    MerCreMer-IRES-Neo
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPIDNB

    Plasmid

    #169901

    Purpose

    Empty dox inducible vector with piggyBac, insulators, and a DTS

    Depositor

    Richard Schneider

    Article

    Chu et al Biol Open. 2020 Oct 6;9(10). pii: bio.05

    Type

    Empty backbone

    Use

    Aivian expression

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dox inducible

    Available since

    Aug. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti-CaMKIIa-eNpHR-EYFP-WPRE

    Plasmid

    #20946

    Purpose

    3rd gen lentiviral expression of halorhodopsin 2.0 (eNpHR) fused to EYFP driven by CaMKIIa promoter for optogenetic inhibition

    Depositor

    Karl Deisseroth

    Article

    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

    Insert

    halorhodopsin
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa

    Available since

    May 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSpyC-GFP

    Plasmid

    #220191

    Purpose

    sgRNA expression vector - pBG42 promoter with GFP in sgRNA site

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    pBG42 promoter with GFP in sgRNA site
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    monomeric superfolder green fluorescent protein

    Promoter

    pBG42

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. GFP-C1-CAMKIIalpha-K42R

    Plasmid

    #21221

    Depositor

    Tobias Meyer

    Article

    Shen et al Science. 1999 Apr 2. 284(5411):162-6.

    Insert

    CAMKIIalpha-K42R (Camk2a Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    K42R (Lab plasmid KS 0007).

    Promoter

    Available since

    July 29, 2009

    Availability

    Academic Institutions and Nonprofits only

22. pEGFP-C1-CaMKIIbeta(K43R)

    Plasmid

    #21225

    Depositor

    Tobias Meyer

    Article

    Shen et al Science. 1999 Apr 2. 284(5411):162-6.

    Insert

    CaMKIIbeta(K43R) (Camk2b Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    CaMKIIbeta(K43R) Lab plasmid CF 0003.

    Promoter

    Available since

    Sept. 4, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAJM.969

    Plasmid

    #108537

    Purpose

    MphRAM + EryR + PMph-YFP Reporter. Ery sensor for use in non-Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    MphRAM + EryR + PMph-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    MphR: N94D

    Promoter

    Available since

    Aug. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCS2-QFGal4-SV40pA

    Plasmid

    #155126

    Purpose

    In vitro transcription of QFGal4 mRNA

    Depositor

    Bret Pearson

    Article

    Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

    Insert

    QFGal4
    

    Use

    Expression in zebrafish

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6

    Available since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA5/FRT/TO_Beta4Gal-HaloTag7-T2A-Lyn11-SNAPf

    Plasmid

    #175524

    Purpose

    Co-expression of HaloTag7 (gogli) and SNAPf-tag (membrane inner leaflet) in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Beta4Gal-HaloTag7 and Lyn11-SNAPf
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRSETb-ffDronpa

    Plasmid

    #78182

    Purpose

    Expresses ffDronpa E. coli strains

    Depositor

    Peter Dedecker

    Article

    Moeyaert et al ACS Nano. 2014 Feb 25;8(2):1664-73. doi:

    Insert

    ffDronpa
    

    Use

    Tags

    6xHis-tag; Xpress tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCS-H2B-citrine

    Plasmid

    #53752

    Purpose

    Contains fusion of human Histone H2B to citrine.

    Depositor

    Sean Megason

    Article

    Megason et al Methods Mol Biol. 2009;546:317-32. doi:

    Insert

    histone H2B (H2BC21 Human)
    

    Use

    Tags

    citrine

    Expression

    Mammalian

    Mutation

    Promoter

    simian CMV IE94

    Available since

    July 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGrDL_SP

    Plasmid

    #83204

    Purpose

    receives miRNA target sequences for testing using the dual luciferase assay system

    Depositor

    Peer Schenk

    Article

    Moyle et al Front Plant Sci. 2017 Sep 20;8:1631. doi

    Insert

    SalI/PstI cloning sites
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. CeNL-Clathrin_pEGFP

    Plasmid

    #89540

    Purpose

    Cyan color variant of bright luminescent protein, fused to clathrin light chain

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    CeNL
    

    Use

    Tags

    Clathrin light chain

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMito-Electra1

    Plasmid

    #179481

    Purpose

    Mitochondrial labeling with blue fluorescent protein Electra1 fused to partial COX8A

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    COX8A-Electra1
    

    Use

    Tags

    Electra1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. HBT_pcoCASphi_version2

    Plasmid

    #197932

    Purpose

    Cloning vector with Arabidopsis codon optimized Casphi-2 protein, with SV40 NLS at the C-terminal

    Depositor

    Steven Jacobsen

    Article

    Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

    Insert

    pcoCasphi-2
    

    Use

    CRISPR

    Tags

    2xNLS-2xFLAG

    Expression

    Plant

    Mutation

    Promoter

    35SPPDK promoter

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. mMaroon-RTK-mMaroon

    Plasmid

    #84359

    Purpose

    FRET acceptor for RhoA donor, flanked with the far-red FP mMaroon

    Depositor

    Ryohei Yasuda

    Article

    Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    mMaroon-Rtk--mMaroon
    

    Use

    Tags

    mMaroon flanking Rtk domain on N and C terminus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pBS-PtH2B

    Plasmid

    #133390

    Purpose

    Piggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR)

    Depositor

    Blair Madison

    Article

    Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    No promoter

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pUCBB-pT7-eGFP

    Plasmid

    #32552

    Depositor

    Claudia Schmidt-dannert

    Article

    Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

    Insert

    eGFP (eGFP )
    

    Use

    Synthetic Biology

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    pT7

    Available since

    Oct. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLV_Lyn11-mTurquoise2-iLID

    Plasmid

    #161000

    Purpose

    Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via N-terminal fusion to lipidated peptide from LYN

    Depositor

    Sean R Collins

    Article

    Natwick et al ACS Synth Biol. 2021 May 21;10(5):1009-1

    Insert

    Lyn11-mTurquoise2-iLID
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCS-H2B-EYFP

    Plasmid

    #53746

    Purpose

    contains fusion of human histone H2B to EYFP in pCS2+ vector.

    Depositor

    Sean Megason

    Article

    Megason et al Methods Mol Biol. 2009;546:317-32. doi:

    Insert

    histone H2B (H2BC21 Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    simian CMV IE94

    Available since

    July 8, 2014

    Availability

    Academic Institutions and Nonprofits only

37. pEGFP-C1-CaMKIIbeta(A303R)

    Plasmid

    #21224

    Depositor

    Tobias Meyer

    Article

    Shen et al Science. 1999 Apr 2. 284(5411):162-6.

    Insert

    CaMKIIbeta(A303R) (Camk2b Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    CaMKIIbeta(A303R) (Lab plasmid CF 0002)

    Promoter

    Available since

    July 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pEntr1a-wGBD-mTurquoise2-wGBD

    Plasmid

    #176136

    Purpose

    Visualization of active Cdc42

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

    Insert

    WAS (WAS Human)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCAG-AsCas12a-2AeGFP

    Plasmid

    #123638

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

    Insert

    AsCas12a
    

    Use

    Tags

    3xHA tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. HaloTag-2xrGBD

    Plasmid

    #176109

    Purpose

    Visualization of active Rho

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

    Insert

    rhotekin (Rtkn Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMVdel

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pYZ146

    Plasmid

    #98406

    Purpose

    Cas9-gRNA leu1 plasmid for leu1 deletion in S. pombe

    Depositor

    Jef Boeke

    Article

    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

    Insert

    gRNA targeting Sp.leu1 (leu1 Fission Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pAC-dCLK

    Plasmid

    #33153

    Depositor

    Michael Rosbash

    Article

    McDonald et al Mol Cell Biol. 2001 Feb;21(4):1207-17.

    Insert

    CLK (Clk Fly)
    

    Use

    Retroviral

    Tags

    6xHIS and V5

    Expression

    Insect

    Mutation

    Promoter

    Actin 5C

    Available since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

43. pLV_mTurquoise2-iLID-CAAX

    Plasmid

    #160999

    Purpose

    Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via C-terminal fusion to CAAX motif from KRAS

    Depositor

    Sean R Collins

    Article

    Natwick et al ACS Synth Biol. 2021 May 21;10(5):1009-1

    Insert

    mTurquoise2-iLID-CAAX
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pCAG-ChroME(S273A)-mRuby2-ST

    Plasmid

    #109128

    Purpose

    Modified cation channelrhodopsin ChroME fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

    Depositor

    Hillel Adesnik

    Article

    Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

    Insert

    ChroME(S273A)-mRuby2-ST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pYZ164

    Plasmid

    #98408

    Purpose

    Cas9-gRNA his3 plasmid for his3 deletion in S. pombe

    Depositor

    Jef Boeke

    Article

    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

    Insert

    gRNA targeting Sp.his3 (his3 Fission Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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46. pPIDCB

    Plasmid

    #169899

    Purpose

    Empty dox inducible vector with piggyBac, insulators, and a DTS

    Depositor

    Richard Schneider

    Article

    Chu et al Biol Open. 2020 Oct 6;9(10). pii: bio.05

    Type

    Empty backbone

    Use

    Avian expression

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dox inducible

    Available since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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47. Carl-ReNL-KDEL_pcDNA3

    Plasmid

    #89535

    Purpose

    Red color variant of bright luminescent protein, targeted to the endoplasmic reticulum

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    ReNL
    

    Use

    Tags

    Calreticulin and KDEL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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48. pCytERM-Electra1

    Plasmid

    #179476

    Purpose

    ER Visualization (OSER assay) Expresses Electra1 in fusion with N-terminal part of p450

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    CytERM-Electra1
    

    Use

    Tags

    Electra1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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49. pTol2-nbt-Electra1-P2A-mScarlet

    Plasmid

    #196113

    Purpose

    zebrafish expression of nbt-Electra1-P2A-mScarlet

    Depositor

    Reinhard Köster

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    nbt-Electra1-P2A-mScarlet
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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50. pActin-Electra1

    Plasmid

    #184941

    Purpose

    beta-actin labelling with blue fluorescent protein Electra1

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    Electra1-beta-Actin
    

    Use

    Tags

    Electra1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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