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12,913 results

Showing: 2751 - 2800 of 12913 results

  6. pX335-EN475

    Plasmid
    #86231
    Purpose
    For transient expression of spCas9-nickase and one sgRNA targeting the mouse CTCF locus. Use together with pX335-EN477.
    Depositor
    Benoit Bruneau
    Article
    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do
    Insert
    spCas9-nickase and sgRNA against mouse CTCF STOP Codon
    Use
    Mouse Targeting
    Expression
    Mammalian
    Available Since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    86231-plasmid-map-sequence-id-177755

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  7. pcDNA3.1 AKT3 HA (CT-gfp)

    Plasmid
    #27293
    Depositor
    Morris Birnbaum
    Insert
    protein kinase B gamma (Akt3 Mouse)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Y473F
    Available Since
    April 7, 2011
    Availability
    Academic Institutions and Nonprofits only

  16. pAAV-iCre-2A-cherry

    Plasmid
    #182246
    Purpose
    Expresses iCre linked to mCherry. The promoter is a fragment of the hdc gene promoter which drives pan neuronal expression.
    Depositor
    William Wisden
    Article
    Miracca et al J Neurosci. 2022 May 31. pii: JNEUROSCI.
    Insert
    pan neuronal gene promoter (hdc) fragment driving iCre recombinase and 2A linked to mCherry
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Promoter
    fragment of histidine decarboxylase gene promoter…
    Available Since
    March 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    182246-plasmid-map-sequence-id-355958

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  22. hEF1α-H2B-mOrange2-IRES-EGFP PGK-Puromycin

    Plasmid
    #99277
    Purpose
    Dual fluorescent reporter - constitutive expression of mOrange2 (549/565) localized in the nucleus, EGFP (488/507) untargeted and visualized in the cytoplasm, and Puromycin resistance
    Depositor
    Jordan Miller
    Article
    Calderon et al Biomater Sci. 2017 Jun 29. doi: 10.1039/
    Inserts
    H2B-mOrange2-IRES-EGFP
    Puro
    Use
    Lentiviral
    Tags
    EGFP, H2B, IRES, PGK, and mOrange2
    Promoter
    EF1 alpha-human and PGK-mouse
    Available Since
    Nov. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99277-plasmid-map-sequence-id-197594

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  24. pB2-neo-TET

    Plasmid
    #51287
    Purpose
    expresses the rodent SINE, B2, using the pol III enhancer sequence of the 7SL gene and tagged with the self splicing intron neo cassette
    Depositor
    Astrid Roy-Engel
    Article
    Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:
    Insert
    sine B2
    Tags
    neoTET cassette with a tetrahymena self-splicing …
    Expression
    Mammalian
    Promoter
    enhancer from 7SL gene
    Available Since
    April 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    51287-plasmid-map-sequence-id-78369

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  25. GFP-Kv2.2

    Plasmid
    #231678
    Purpose
    Expression of mouse Kcnb2 fused with EGFP in C-terminal.
    Depositor
    Federico Sesti
    Insert
    Kcnb2 (Kcnb2 Mouse)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    None
    Available Since
    March 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    231678-plasmid-map-sequence-id-463617

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  29. pET28a-mOct4 Full length(FL)

    Plasmid
    #206394
    Purpose
    Expresses Oct4 with a 6xHis tag for protein purification
    Depositor
    Ralf Jauch
    Insert
    Oct4 (Pou5f1 Mouse)
    Tags
    6xHis
    Expression
    Bacterial
    Available Since
    Oct. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    206394-plasmid-map-sequence-id-409984

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  38. pX335-EN477

    Plasmid
    #86232
    Purpose
    For transient expression of spCas9-nickase and one sgRNA targeting the mouse CTCF locus. Use together with pX335-EN475.
    Depositor
    Benoit Bruneau
    Article
    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do
    Insert
    spCas9-nickase and sgRNA against mouse CTCF STOP Codon
    Use
    Mouse Targeting
    Expression
    Mammalian
    Available Since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    86232-plasmid-map-sequence-id-177242

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  40. pPB-tetO(hCMV1)-HA-Tet1mHxD(201R2)-IV

    Plasmid
    #102422
    Purpose
    Inducible expression of siRNA resistant mouse Tet1-201 (ENSMUST00000050826.13) with mutated catalytic domain (H1620Y & D1622A), HA-tag and IRES-Venus in piggyBac (PB) transposon vector
    Depositor
    Kian Peng Koh
    Article
    Khoueiry et al Nat Genet. 2017 Jul;49(7):1061-1072. doi
    Insert
    Tet1 catalytic domain mutant (Tet1 Mouse)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    H1620Y and D1622A mutations in Tet1 catalytic dom…
    Promoter
    TetO-CMV
    Available Since
    Nov. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    102422-plasmid-map-sequence-id-198789

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  44. pGL3 basic Mut E2

    Plasmid
    #48748
    Purpose
    Expression of luciferase driven by mPer2 promoter fragment containing mutated E-box2 (bases -112 to +98 with respect to transcription start site at +1)
    Depositor
    Joseph Takahashi
    Article
    Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10
    Insert
    mPer2 promoter/enhancer region containing mutated E-box2 (-112 to +98)
    Use
    Luciferase
    Mutation
    Mutated E-box2 sequence from CACGTT to GCTAGT
    Available Since
    Dec. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
    48748-plasmid-map-sequence-id-448240

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Showing: 2751 - 2800 of 12913 results