We narrowed to 9,340 results for: UTY

1. AAV-P3-evoCjCas9-eAID_gRNA

   Plasmid

   #202562

   Purpose

   P3-evoCjCas9-eAID and hU6-BsmBI-gRNA in AAV2 backbone (ITRs)

   Depositor

   Gerald Schwank

   Article

   Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

   Insert

   evoCjCas9-eAID
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBT13-splitC

   Plasmid

   #122596

   Purpose

   clone M13 phage by SapI Golden Gate

   Depositor

   David Liu

   Article

   Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

   Insert

   M13 bacteriophage genes
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMK449 (BromoTag-mClover-BSD)

   Plasmid

   #214379

   Purpose

   BromoTag-mClover for C-terminal tagging

   Depositor

   Masato Kanemaki

   Article

   Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

   Insert

   BromoTag-mClover-BSD
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. GST-NDP52

   Plasmid

   #171422

   Purpose

   For the purifcation of NDP52 protein

   Depositor

   James Hurley

   Article

   Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

   Insert

   NDP52 (CALCOCO2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. mOtop1_pcDNA3

   Plasmid

   #114677

   Purpose

   This construct was used to express mouse Otop1 in HEK-293 cells

   Depositor

   Emily Liman

   Article

   Tu et al Science. 2018 Mar 2;359(6379):1047-1050.

   Insert

   Otopetrin 1 (Otop1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   SNP (rs49325632): Glycine 396 to Alanine

   Promoter

   CMV

   Available since

   July 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFW5000

   Plasmid

   #224214

   Purpose

   E. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), p15A (E. coli replication origin), tetQ

   Depositor

   Ophelia Venturelli

   Article

   Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Halo-Cyto

   Plasmid

   #124314

   Purpose

   Expresses cytosolic specific HaloTag protein

   Depositor

   Jin Wang

   Article

   Jiang et al Antioxid Redox Signal. 2018 Oct 25. doi:

   Insert

   Halo tag
   

   Use

   Tags

   Cytoplasm specific seq

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHAGE-TO-nls-st1dCas9-3nls-3XGFP-nls

   Plasmid

   #64112

   Purpose

   Expression of St1 dCas9-3XGFP in mammalian cells

   Depositor

   Thoru Pederson

   Article

   Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

   Insert

   St1 dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3XsfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TO

   Available since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCS-PesaRlux

   Plasmid

   #47640

   Purpose

   wt esaR promoter in pCS26

   Depositor

   Cynthia Collins

   Article

   Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

   Insert

   PesaR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PesaR

   Available since

   Oct. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMVV207

    Plasmid

    #215343

    Purpose

    gRNA expression for S. stutzeri nifL deletion on an RSF1010 backbone

    Depositor

    Brian Pfleger

    Article

    Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

    Insert

    S. stutzeri nifL gRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    None

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET-BoNT/X

    Plasmid

    #168017

    Purpose

    Expresses BoNT/X-LC(1-439)-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    BoNT/X-LC(1-439)-6xHis
    

    Use

    Tags

    Hisx6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pOPS0377

    Plasmid

    #115509

    Purpose

    Reporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing sfGFP

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    sfGFP (pOGG037) and T-pharma (pOGG003) assembled in pOGG026
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Constitutive promoter pNeo pOGG001

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFW6000

    Plasmid

    #224215

    Purpose

    Bacteroides genomic editing tool; aTc inducible-Fncpf1

    Depositor

    Ophelia Venturelli

    Article

    Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

    Insert

    Fncpf1
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Prab-3::NLS::GCaMP6s

    Plasmid

    #68119

    Purpose

    Nuclear Localized Fluorescent Calcium Indicator for pan-neuronal expression in C. Elegans

    Depositor

    Andrew Leifer

    Article

    Nguyen et al Proc Natl Acad Sci U S A. 2015 Dec 28. p

    Insert

    rab-3::NLS::GCaMP6s
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    rab-3

    Available since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET-E(4130)A2

    Plasmid

    #168021

    Purpose

    Expresses E(4130)A2-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    E(4130)A2-6xHis
    

    Use

    Tags

    Hisx6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAd-Klf4

    Plasmid

    #19770

    Depositor

    Konrad Hochedlinger

    Article

    Stadtfeld et al Science. 2008 Sep 25. ():.

    Insert

    Klf4 (Klf4 Mouse)
    

    Use

    Adenoviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. APT3-Ykt6

    Plasmid

    #168003

    Purpose

    positive selection PACE Accessory Plasmid

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    Ykt6(L161-L177)-T3'PAP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pL2-1 ConjEpi TpEF2nat TpEF2NoSQEVenusThrFLAG

    Plasmid

    #139961

    Purpose

    Over-expression of heterologus squalene epoxidase from Nannochloropsis oceanica

    Depositor

    Raffaela Abbriano

    Article

    Jaramillo-Madrid et al Pharmaceuticals (Basel). 2020 Dec 21;13(

    Insert

    ConjEpi TpEF2_nat_tTpEF2 TpEF2_NoSQEVenusThrFLAG_tTpEF2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28-2H12.D11

    Plasmid

    #221145

    Purpose

    His6-tagged cdGreen2 expressed from T7 promoter (Internal Lab ID: UJ11357)

    Depositor

    Urs Jenal

    Article

    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

    Insert

    cdGreen2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. His6-PRC1

    Plasmid

    #69111

    Purpose

    For bacterial expression of recombinant human PRC1 tagged with His.

    Depositor

    Stephen Royle

    Article

    Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

    Insert

    Protein Regulator of Cytokinesis 1 (PRC1 Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBT371

    Plasmid

    #125612

    Purpose

    Expresses CDA1-BE4max-NG in mammalian cells

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    CDA1-BE4max-NG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMK455 (dTAG-mClover-BSD)

    Plasmid

    #214385

    Purpose

    dTAG-mClover for C-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    dTAG-mClover-BSD
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMK456 (dTAG-mCherry-BSD)

    Plasmid

    #214386

    Purpose

    dTAG-mCherry for C-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    dTAG-mCherry-BSD
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. MBP-TEVcs(ENLYFQ/G)-His6-TEVΔ(220-242)-R5

    Plasmid

    #135454

    Purpose

    Bacterial expression of TEVΔ protease

    Depositor

    Alice Ting

    Article

    Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

    Insert

    MBP-TEVcs(ENLYFQ/G)-His6-TEVΔ(220-242)-R5
    

    Use

    Tags

    MBP, His6

    Expression

    Bacterial

    Mutation

    S219V mutation improves stability. Truncated form…

    Promoter

    tac promoter

    Available since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. p2H12

    Plasmid

    #221142

    Purpose

    cdGreen expressed from an aTc-inducible promoter (Internal Lab ID: UJ11204)

    Depositor

    Urs Jenal

    Article

    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

    Insert

    cdGreen
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptet

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCMV4a-SIRT6-FLAG

    Plasmid

    #102322

    Purpose

    Human SIRT6 with FLAG tag for mammalian expression

    Depositor

    Hening Lin

    Article

    Zhang et al Nat Chem Biol. 2016 Aug;12(8):614-20. do

    Insert

    SIRT6 (SIRT6 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pUb RxCas13d + RxCas13d guide RNA

    Plasmid

    #176303

    Purpose

    Expresses RxCas13d and its associated guide RNA in Drosophila cells

    Depositor

    Jeremy Wilusz

    Article

    Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

    Insert

    RxCas13d
    

    Use

    Tags

    HA tag

    Expression

    Insect

    Mutation

    Promoter

    Ubi-p63e

    Available since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. AAV.hSyn.GLPLight1

    Plasmid

    #187466

    Purpose

    Expresses GLPLight1 in neurons

    Depositor

    Tommaso Patriarchi

    Article

    Duffet et al Elife. 2023 Jun 2;12:RP86628. doi: 10.75

    Insert

    GLPLight1
    

    Use

    AAV

    Tags

    Flag tag

    Expression

    Mutation

    Promoter

    human Synapsin-1

    Available since

    Feb. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pPy-CAG-Cre::ERT2-IRES-BSD

    Plasmid

    #48760

    Purpose

    CAG promoter driving Cre-ERT2 fusion-IRES-Blasticidin resistance

    Depositor

    Austin Smith

    Article

    Betschinger et al Cell. 2013 Apr 11;153(2):335-47. doi: 10

    Insert

    Cre::ERT2
    

    Use

    Ppy

    Tags

    ERT2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCMV-SpCas9-P2A-puro

    Plasmid

    #214358

    Purpose

    Expresses SpCas9 and puromycin resistance genes

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SpCas9 and puromycin resistance genes
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pHS-139

    Plasmid

    #195461

    Purpose

    Lentiviral Integratable, GZV-inducible expression of synZiFTR: ZF10-p65

    Depositor

    Ahmad Khalil

    Article

    Li et al Science. 2022 Dec 16;378(6625):1227-1234

    Insert

    pSFFV - ZF10 - NS3 - p65
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3.1 5HT2A_NB2_1

    Plasmid

    #127690

    Purpose

    Plasmid contains a positive allosteric modulating nanobody the binds to the cytoplasmic surface of 5HT2A.

    Depositor

    Justin English, Bryan Roth

    Article

    English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

    Insert

    5HT2A_NB2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pJB042 (FtsZ-mEos2)

    Plasmid

    #49764

    Purpose

    Inducible expression of FtsZ-mEos2 in bacteria

    Depositor

    Jie Xiao

    Article

    Buss et al Mol Microbiol. 2013 Sep;89(6):1099-120.

    Insert

    FtsZ-mEos2
    

    Use

    Tags

    mEos2

    Expression

    Bacterial

    Mutation

    Promoter

    T5-lac

    Available since

    March 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pSpyC-GFP

    Plasmid

    #220191

    Purpose

    sgRNA expression vector - pBG42 promoter with GFP in sgRNA site

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    pBG42 promoter with GFP in sgRNA site
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    monomeric superfolder green fluorescent protein

    Promoter

    pBG42

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCF CREB M1

    Plasmid

    #22969

    Depositor

    Marc Montminy

    Article

    Du et al Mol Cell Biol. 2000 Jun . 20(12):4320-7.

    Insert

    CREB (Creb1 Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Changed Serine 133 to Alanine

    Promoter

    Available since

    Feb. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNAintron-LASV-GPC

    Plasmid

    #215690

    Purpose

    Produces the Lassa virus glycoprotein

    Depositor

    Melinda Brindley

    Article

    Acciani et al J Virol. 2017 Aug 24;91(18):e00574-17. d

    Insert

    LASV GP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

37. pSGKP_AcsgRNA

    Plasmid

    #203807

    Purpose

    pSGKP-based plasmid containing a J23119-driven sgRNA cassette, with replaceable spacer (BsaI-restriction sites). Kanamycin resistance.

    Depositor

    Quanjiang Ji

    Article

    Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

    Insert

    AcgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    J23119

    Available since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pUb dPspCas13b + PspCas13b guide RNA

    Plasmid

    #176306

    Purpose

    Expresses catalytic dead PspCas13b and its associated guide RNA in Drosophila cells

    Depositor

    Jeremy Wilusz

    Article

    Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

    Insert

    PspCas13b
    

    Use

    Tags

    HA tag

    Expression

    Insect

    Mutation

    H133A, H1058A

    Promoter

    Ubi-p63e

    Available since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. mOtop2_pGEMHE

    Plasmid

    #114675

    Purpose

    This construct was used to express mouse Otop2 in Xenopus laevis oocytes

    Depositor

    Emily Liman

    Article

    Tu et al Science. 2018 Mar 2;359(6379):1047-1050.

    Insert

    Otopetrin 2 (Otop2 Mouse)
    

    Use

    For generating the mrna, which can highly express…

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCAG>nls-Cas9-nls-2A-Citrine-EcoRI/NotI

    Plasmid

    #169097

    Purpose

    Cas9 and Citrine expression in transfected cells

    Depositor

    Marianne Bronner

    Article

    Gandhi et al Development. 2021 Mar 9. pii: dev.193565

    Insert

    Cas9-2A-Citrine
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pLv-CMV-MyoD

    Plasmid

    #26808

    Depositor

    Jeffrey Chamberlain

    Article

    Kimura et al Hum Mol Genet. 2008 Aug 15. 17(16):2507-

    Insert

    MyoD (Myod1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pCMB-TMEr

    Plasmid

    #61208

    Purpose

    Fluorescent reporter for plasma membrane and peri-arbuscular membrane MtBCPsp-mCherry-BCP expressed under MtBCP1 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    MtBCPsp-mCherry-BCP
    

    Use

    Tags

    mCherry

    Expression

    Plant

    Mutation

    Promoter

    MtBCP1

    Available since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. Ubiquitin K63C

    Plasmid

    #86589

    Purpose

    Bacterial expression of Ubiquitin K63C for cross linking studies

    Depositor

    Sharon Rozovsky

    Article

    Liu et al J Am Chem Soc. 2017 Feb 10. doi: 10.1021

    Insert

    Ubiquitin (UBC Human)
    

    Use

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    TEV site; K63C

    Promoter

    Tac

    Available since

    Feb. 24, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

44. pKL004

    Plasmid

    #98920

    Purpose

    Constitutively expresses the calcium sensor GCaMP6f tethered to mRuby3 under the Sigma 70 promoter in E. coli.

    Depositor

    Joel Kralj

    Article

    Bruni et al Proc Natl Acad Sci U S A. 2017 Aug 29;11

    Insert

    GCaMP6f tethered to mRuby
    

    Use

    Tags

    His and mRuby3

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23118 - Strong Constitutive

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. HA-DN-14-3-3 zeta (R56A/R60A)

    Plasmid

    #116889

    Purpose

    Expresses HA-tagged human dominant-negative 14-3-3 zeta (R56A/R60A) in mammalian cells

    Depositor

    FENG-QIAN Li, Ken-Ichi Takemaru

    Article

    Li et al J Cell Biol. 2008 Jun 30;181(7):1141-54.

    Insert

    DN-14-3-3 zeta (R56A/R60A) (YWHAZ Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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46. pTNSQCas

    Plasmid

    #219434

    Purpose

    CRISPR-associated transposases from V. cholerae and used for RNA guided cargo insertion in Vibrio natriegens

    Depositor

    Sheng Yang

    Article

    Xu et al Biotechnol Bioeng. 2024 Mar;121(3):1163-

    Insert

    TnsA, TnsB, TnsC, TniQ, Cas8, Cas7, Cas6
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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47. pDonor-LoxP

    Plasmid

    #219436

    Purpose

    CRISPR-associated transposases from V. cholerae and used for RNA guided cargo insertion in Vibrio natriegens

    Depositor

    Sheng Yang

    Article

    Xu et al Biotechnol Bioeng. 2024 Mar;121(3):1163-

    Insert

    two transposon ends, LoxP-terminator-LoxP gene cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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48. pGH183_MS2-AIDΔDead-Hygro

    Plasmid

    #85414

    Purpose

    lentiviral expression vector for MS2-AIDΔDead fusion protein with hygromycin B selectable marker

    Depositor

    Michael Bassik

    Article

    Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    MS2-AIDΔDead and Hygromycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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49. pMK451 (BSD-P2A-dTAG)

    Plasmid

    #214381

    Purpose

    dTAG for N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    BSD-P2A-dTAG
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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50. pET-BoNT/E

    Plasmid

    #168020

    Purpose

    Expresses BoNT/E-LC(1-411)-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    BoNT/E-LC(1-411)-6xHis
    

    Use

    Tags

    Hisx6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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