We narrowed to 10,116 results for: gnas

Showing: 2901 - 2950 of 10116 results

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pcDNA3.1(+)-blueAKAR

Plasmid

#118470

Purpose

cpBFP-based single-color biosensor for monitoring Protein Kinase A activity.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

blueAKAR

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV_2pabPAC(Y72L)

Plasmid

#158970

Purpose

A negative control for AAV-mediated 2P-activatable bPAC

Depositor

Michiyuki Matsuda

Article

Kinjo et al ACS Chem Biol. 2020 Oct 19. doi: 10.1021

Insert

2pabPACY72L

Use

AAV

Promoter

CMV

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5-HA GST RagA 66L

Plasmid

#19300

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding A (RRAGA Human)

Tags

GST and HA

Expression

Mammalian

Mutation

Q66L

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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sAB-MBP

Plasmid

#204736

Purpose

synthetic antigen-binding fragment specifically recognizing bacteria maltose-binding protein

Depositor

Minglei Zhao

Article

Yu et al Nat Chem Biol. 2021 Aug;17(8):896-905. d

Insert

synthetic antigen-binding fragment recognizing bacteria maltose-binding protein

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX6-Smad3 WT

Plasmid

#27010

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Tags

GST

Expression

Bacterial

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pIRES2-EGFP Rac2

Plasmid

#12193

Depositor

Gary Johnson

Article

Abell et al J Cell Sci. 2004 Jan 15. 117(Pt 2):243-5

Insert

Rac2 (RAC2 Human)

Expression

Mammalian

Available Since

Oct. 3, 2006

Availability

Academic Institutions and Nonprofits only

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Lepr peak reporter-eGFP

Plasmid

#196584

Purpose

Cis-regulatory region of mouse Lepr gene driven a eGFP reporter with hGFP driven rtTA3

Depositor

Elaine Fuchs

Article

Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

Insert

eGFP

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

SV40

Available Since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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RCAN1

Plasmid

#65413

Purpose

Full length RCAN1 expression with CMV promoter. Flag tagged.

Depositor

Jeffery Molkentin

Article

Liu et al Nat Cell Biol. 2009 Feb;11(2):154-61. do

Insert

RCAN1 (Rcan1 Mouse)

Tags

Flag tagged

Expression

Mammalian

Promoter

CMV

Available Since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

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pHAGE-CMV-Rheb-IRES-eGFP-W

Plasmid

#32519

Depositor

Mustafa Sahin

Article

Nie et al Nat Neurosci. 2010 Feb;13(2):163-72. Epu

Insert

Rheb (Rheb Rat)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only
pLCRISPR-CMV

Plasmid

#102609

Purpose

Control lentivector expressing Cas9 and gRNA scaffold

Depositor

Nicolas Manel

Article

Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 19, 2018

Availability

Academic Institutions and Nonprofits only

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P023-mPB-synHEK3-BsaI

Plasmid

#239975

Purpose

PiggyBac plasmid for synthetic DNA Tape (HEK3 locus)

Depositor

Jay Shendure

Article

Chen et al Nature. 2024 Aug;632(8027):1073-1081. do

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pSicoR (Puro) p65 shRNA1

Plasmid

#22507

Depositor

Tyler Jacks

Article

Meylan et al Nature. 2009 Nov 5;462(7269):104-7. doi:

Insert

p65 shRNA1 (Rela Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

Nov. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pCS2-FLAG-hEcto/Tif1g

Plasmid

#20902

Depositor

Stefano Piccolo

Article

Dupont et al Cell. 2005 Apr 8. 121(1):87-99.

Insert

Ectodermin (TRIM33 Human)

Tags

FLAG

Expression

Mammalian

Mutation

N-terminal deletion of 37 aminoacids; Ectodermin …

Available Since

April 22, 2009

Availability

Academic Institutions and Nonprofits only
pCS2 stabilized mutant Beta Catenin-GFP

Plasmid

#29684

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

beta catenin (ctnnb1.L Frog)

Tags

GFP

Expression

Mammalian

Mutation

S33A, S37A, T41A

Available Since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only
pARF6-YFP

Plasmid

#11389

Depositor

Joel Swanson

Article

Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

Insert

ADP Ribosylation Factor 6 (ARF6 Human)

Tags

YFP

Expression

Mammalian

Available Since

March 3, 2006

Availability

Academic Institutions and Nonprofits only

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Plasma membrane-targeted optodPLD

Plasmid

#140061

Purpose

Encodes CRY2-mCherry-dPLD-P2A-CIBN-CAAX for mammalian expression

Depositor

Jeremy Baskin

Article

Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Phospholipase D

Tags

CAAX, CIBN, CRY2, P2A, and mCherry

Expression

Mammalian

Mutation

H170A (catalytic dead)

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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Flag pLJM1 RagD 121L

Plasmid

#19318

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding D (RRAGD Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Q121L

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only
pWZLblasti-CTR1

Plasmid

#53157

Purpose

Expresses human CTR1 from blasticidin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

CTR1 (SLC31A1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

June 24, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-M2-TLR2

Plasmid

#12291

Depositor

Bruce Beutler

Article

Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

TLR2 (Tlr2 Mouse)

Tags

FLAG (FLAG-M2)

Expression

Mammalian

Mutation

M82I and H202Y; signal peptide (aa1-23) replaced …

Available Since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only
pPyCAG-mKate2-3xNLS-IRES-Pac

Plasmid

#183618

Purpose

Mammalian expression vector for mKate2-3xNLS from the CAG promoter. Confers puromycin resistance.

Depositor

Sally Lowell

Article

Malaguti et al Elife. 2013 Dec 17;2:e01197. doi: 10.755

Insert

mKate2-3xNLS

Tags

3xNLS

Expression

Mammalian

Promoter

CAG

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pNTI822

Plasmid

#216631

Purpose

Tet-inducible cpTurboID (V5) for Sleeping Beauty transposon system

Depositor

Nicholas Ingolia

Article

Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

Insert

cpTurboID

Use

Sleeping beauty transposon system

Tags

V5

Expression

Mammalian

Mutation

L120P

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
YF-RhoA(CA)

Plasmid

#20153

Depositor

Tobias Meyer

Article

Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

Insert

YFP-FKBP-RhoA(CA) (RHOA Human)

Expression

Mammalian

Available Since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-SH3BP4

Plasmid

#46891

Purpose

expresses HA tagged SH3BP4 in mammalian cells

Depositor

Do-Hyung Kim

Article

Kim et al Mol Cell. 2012 Jun 29;46(6):833-46. doi:

Insert

SH3BP4 (SH3BP4 Human)

Tags

HA

Expression

Mammalian

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pCMV5B-TFGbeta receptor II K277R

Plasmid

#11762

Depositor

Joan Massague, Jeff Wrana

Article

Wrana et al Cell. 1992 Dec 11. 71(6):1003-14.

Insert

TGFbeta receptor II (TGFBR2 Human)

Tags

His

Expression

Mammalian

Mutation

K277R: kinase dead

Promoter

CMV

Available Since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pCMV5-ALK2 Q207D

Plasmid

#11740

Depositor

Jeff Wrana

Article

Macias-Silva et al J Biol Chem. 1998 Oct 2. 273(40):25628-3

Insert

ALK2 (ACVR1 Human)

Expression

Mammalian

Mutation

Q207D: activated receptor

Available Since

Aug. 4, 2006

Availability

Industry, Academic Institutions, and Nonprofits
pfli1

Plasmid

#22413

Depositor

Nathan Lawson

Article

Lawson et al Development. 2001 Oct . 128(19):3675-83.

Insert

fli1 (fli1 Zebrafish)

Use

Riboprobe

Available Since

Dec. 14, 2009

Availability

Academic Institutions and Nonprofits only
HA.PKD.S738E/S742E

Plasmid

#10810

Depositor

Alex Toker

Article

Storz et al Mol Cell Biol. 2004 Apr . 24(7):2614-26.

Insert

PKD (PRKD1 Human)

Tags

HA

Expression

Mammalian

Mutation

Activation loop active: S738E and S742E (bp 2212-…

Promoter

CMV

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only
pCDNA3 mSmoothened WT

Plasmid

#126408

Purpose

Full length mouse Smoothened cDNA. Expression of Smoothened in mammalian cell lines.

Depositor

Stacey Ogden

Article

Marada et al PLoS Genet. 2015 Aug 20;11(8):e1005473.

Insert

m-Smoothened (Smo Mouse)

Expression

Mammalian

Available Since

June 13, 2019

Availability

Academic Institutions and Nonprofits only

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FUGW-UBC-EGFP-2A-BoNT-B (LC)

Plasmid

#185722

Purpose

Lentiviral expression of GFP and botulinum neurotoxin type B light chain from UbC promoter

Depositor

Jun Ding

Article

Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

Insert

botulinum neurotoxin type B light chain

Use

Lentiviral

Expression

Mammalian

Promoter

hUbC

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHL 100

Plasmid

#52989

Purpose

pLlac0-1:OxyS-mCherry, pLtetO-1:fhlA::gfp

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Inserts

OxyS sRNA
fhlA

Use

Synthetic Biology

Tags

GFP and RBS-mCherry

Expression

Bacterial

Mutation

−309 to +96 bp relative to start codon

Promoter

pLlac0-1 and pLtetO-1

Available Since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
pRK5-HA GST RagA 21L

Plasmid

#19299

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding A (RRAGA Human)

Tags

GST and HA

Expression

Mammalian

Mutation

T21L

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only
CMV-PKCb1-mEGFP-N1

Plasmid

#112265

Purpose

ITRACKbeta/IDOCKSbeta Donor construct

Depositor

Ryohei Yasuda

Article

Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

Insert

protein kinase c beta (Prkcb Mouse)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpCas9(BB)-2A-Puro-SMCR8

Plasmid

#83440

Purpose

Expresses Cas9 and a gRNA targeting SMCR8

Depositor

Shawn Ferguson

Article

Amick et al Mol Biol Cell. 2016 Aug 24. pii: mbc.E16

Insert

gRNA targeting human SMCR8 (SMCR8 Human)

Expression

Mammalian

Promoter

U6

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMuLE (R4-R3) PRB

Plasmid

#206164

Purpose

pMuLE R4-R3 entry plasmid containing human full length PRB gene, for 3-component Gateway assembly

Depositor

Renee van Amerongen

Article

Aarts et al J Mammary Gland Biol Neoplasia. 2023 Nov

Insert

Progesteron receptor B (PGR Human)

Use

Gateway cloning vector

Promoter

-

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pNES-iRFP-C1

Plasmid

#116863

Purpose

cytosolic iRFP

Depositor

Gerry Hammond

Article

Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

Insert

iRFP713

Tags

X.leavis map2k1.L(32-44)

Expression

Mammalian

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pBabe-puro-Smad4-Flag

Plasmid

#37041

Depositor

Sam Thiagalingam

Article

Papageorgis et al Cancer Res. 2011 Feb 1;71(3):998-1008. E

Insert

Smad4 (SMAD4 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Promoter

5'LTR

Available Since

March 1, 2013

Availability

Academic Institutions and Nonprofits only
pLKO-shSmad4

Plasmid

#37046

Depositor

Sam Thiagalingam

Article

Papageorgis et al Cancer Res. 2010 Feb 1;70(3):968-78. Epu

Insert

Smad4 (SMAD4 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Jan. 18, 2013

Availability

Academic Institutions and Nonprofits only
pBW101ParsR-gfp

Plasmid

#78636

Purpose

pSB3K3 carrying J101-rbs32-arsR-t-ParsR-30gfp-t

Depositor

Baojun Wang

Article

Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

Insert

J101-rbs32-arsR-t-ParsR-30gfp-t

Use

Synthetic Biology

Promoter

see insert

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only
pLKO.1 RagC shRNA #1

Plasmid

#26629

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

RagC shRNA (RRAGC Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Oct. 22, 2010

Availability

Academic Institutions and Nonprofits only
p133Tol2-Olactb:MCS, hsp70l-mcherry-CreERT2

Plasmid

#227775

Purpose

Expression of beta actin driven BFP with multiple cloning site at 3' end. Also contains heat-shock activated mCherry reporter and CreERT2.

Depositor

Bushra Raj

Article

Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

Inserts

BFP-MCS
mCherry-t2A-CreERT2

Use

Zebrafish expression

Promoter

Olactb and hsp70l

Available Since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
nls-Flamindo2

Plasmid

#73939

Purpose

Expresses biosensor for cAMP in mammalian cells with nuclear localization signal

Depositor

Tetsuya Kitaguchi

Article

Odaka et al PLoS One. 2014 Jun 24;9(6):e100252. doi:

Insert

nls-Flamindo2

Expression

Mammalian

Promoter

CMV

Available Since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP-MCPIP1

Plasmid

#127460

Purpose

Expressing EGFP-MCPIP1 fusion protein in mammalian cells

Depositor

Mingui Fu

Article

Liang et al J Exp Med. 2010 Dec 20;207(13):2959-73.

Insert

MCPIP1 (ZC3H12A Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2 hSmad1-WT

Plasmid

#22989

Depositor

Edward De Robertis

Article

Pera et al Genes Dev. 2003 Dec 15. 17(24):3023-8.

Insert

Smad1 (SMAD1 Human)

Tags

FLAG

Expression

Mammalian

Available Since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
vYM073_PB-pEF-osTIR1-T2A-mCherry-AID-Flag-BlastR

Plasmid

#160042

Purpose

For the auxin receiver that report on auxin.

Depositor

Michael Elowitz

Article

Ma et al Cell. 2022 Mar 17;185(6):967-979.e12. do

Inserts

osTIR1
BlastR

Use

Luciferase

Tags

mAID and mCherry

Expression

Mammalian

Available Since

April 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-Wnt4-V5

Plasmid

#35929

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt4 (WNT4 Human)

Tags

V5

Expression

Mammalian

Mutation

Silent Mutation C (492) --> T

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
Omega Destination-CMV::ELuc:bGH

Plasmid

#124528

Purpose

Omega destination vector, that has a CMV:ELUC:bgH in the backbone

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Type

Empty backbone

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
p155ME-loxP-dsR-loxP-Cas9-t2A-GFP

Plasmid

#227774

Purpose

Middle-entry vector with dsRed flanked by loxP sites and spCas9-GFP for Gateway cloning

Depositor

Bushra Raj

Article

Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

Insert

loxP-dsR-loxP

Use

CRISPR

Available Since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pEB2_mScarletI_mVenusNB-MrkH

Plasmid

#182291

Purpose

expresses c-di-GMP biosensor in E. coli

Depositor

Howard Berg

Article

Vrabioiu et al Proc Natl Acad Sci U S A. 2022 Feb 8;119

Insert

mVenusNB-MrkH

Tags

mVenusNB internal fusion to MrkH

Expression

Bacterial

Promoter

proC

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
TagBFP2-PIP4K2AD273K

Plasmid

#202727

Purpose

expresses fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mTagBFP2:PIP4K2A(D266K) (PIP4K2A Human)

Expression

Mammalian

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP-Lyn11C32S-PIP4K2A

Plasmid

#202748

Purpose

weakly plasma membrane targeted fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

EGFP:LYN (1-11) (C3S):PIP4K2A (PIP4K2A Human)

Expression

Mammalian

Available Since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 10,116 results for: gnas