8,964 results

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pRK-ATF4

Plasmid

#26114

Depositor

Yihong Ye

Article

Wang et al Proc Natl Acad Sci U S A. 2009 Feb 17. 1

Insert

ATF4 (ATF4 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Dec. 2, 2010

Availability

Academic Institutions and Nonprofits only

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pAAV-MBP-2xNLS-tdTomato

Plasmid

#104054

Purpose

An AAV genome encoding expression of the nuclear localized fluorescent protein tdTomato from the MBP promoter

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Insert

2xNLS-tdTomato

Use

AAV

Tags

NLS

Promoter

MBP

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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TetOn-mCherry-eGFP-RAMP4

Plasmid

#109014

Purpose

Tet-On construct to induce expression of tandem eGFP-mCherry tagged RAMP4 (ER marker)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

RAMP4 (SERP1 Human)

Use

Lentiviral

Tags

mCherry-eGFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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LRG (Lenti_sgRNA_EFS_GFP)

Plasmid

#65656

Purpose

Lentiviral introduction of sgRNA constitute expression linked with GFP marker into mammalian cell line.

Depositor

Christopher Vakoc

Article

Shi et al Nat Biotechnol. 2015 May 11. doi: 10.103

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter-driven sgRNA expression and EFS promo…

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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eIF2a 1

Plasmid

#21807

Depositor

David Ron

Insert

eIF2a (EIF2S1 Human)

Expression

Mammalian

Available Since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pRK5 bovine GRK2

Plasmid

#14691

Depositor

Robert Lefkowitz

Article

Pitcher et al J Biol Chem. 1999 Dec 3. 274(49):34531-4

Insert

GRK2 (GRK2 Bovine)

Expression

Mammalian

Available Since

June 19, 2007

Availability

Academic Institutions and Nonprofits only
pVSV eGFP RABV-G

Plasmid

#31833

Depositor

Connie Cepko

Article

Beier et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

Insert

VSV genome

Expression

Bacterial

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

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pLentiCas9-BFP

Plasmid

#78545

Purpose

Fusion protein Cas9+BFP

Depositor

Roderic Guigo, Rory Johnson

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

BFP

Use

Lentiviral

Expression

Mammalian

Promoter

Fusion protein with Cas9

Available Since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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pFA6a-TurboID-3MYC-kanMX6 (pFB1420)

Plasmid

#126049

Purpose

For C-terminal tagging with TurboID in yeast including geneticin-resistant marker

Depositor

François Bachand

Article

Larochelle et al J Cell Sci. 2019 May 7. pii: jcs.232249.

Insert

TurboID

Use

Pcr-based c-terminal tagging

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pGH125_dCas9-Blast

Plasmid

#85417

Purpose

lentiviral expression vector for dCas9 with Blasticidin selectable marker

Depositor

Michael Bassik

Article

Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

dCas9 and Blasticidin resistance

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pHAGE-BRAF-V600E

Plasmid

#116204

Purpose

Lentiviral expression of BRAF V600E

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Mutation

V600E

Available Since

Jan. 11, 2019

Availability

Academic Institutions and Nonprofits only

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myc-NLRC5

Plasmid

#37509

Depositor

Thomas Kufer

Article

Neerincx et al J Immunol. 2012 May 15;188(10):4940-50.

Insert

NLRC5 (NLRC5 Human)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pNAHA

Plasmid

#44169

Depositor

John Olsen

Article

Patel et al J Gene Med. 2013 Jan;15(1):51-62. doi: 1

Inserts

hemagglutinin gene
neuraminidase gene

Expression

Mammalian

Mutation

T55A

Promoter

CMV enhancer/human Ferritin light chain promoter …

Available Since

May 17, 2013

Availability

Academic Institutions and Nonprofits only

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MSCV IRES Luciferase

Plasmid

#18760

Depositor

Scott Lowe

Type

Empty backbone

Use

Luciferase and Retroviral

Expression

Mammalian

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

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pGL4-ERSE1-luc2P-Hygro

Plasmid

#101789

Purpose

Luferase reporter plasmid containing three tandem repeats of the ER stress response element-I (ERSE-I)

Depositor

Seiichi Oyadomari

Article

Fujita et al Acta Med Okayama. 2020 Jun;74(3):199-208

Insert

3xERSE-I

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Promoter

minimal TATA-box promoter with low basal activity

Available Since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_antiHer24D5-7_synNotch_Gal4VP64

Plasmid

#85424

Purpose

Lentiviral vector for expression of anti-Her2 scFv 24D5-7 Gal4VP64 synthetic Notch

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

anti-Her2_4D5-7_synNotch

Use

Lentiviral

Promoter

PGK

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pCIP-AMPKa1_WT

Plasmid

#79010

Purpose

For mammalian expression of WT AMPK alpha1.

Depositor

Reuben Shaw

Article

Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

Insert

AMPK alpha 1 (PRKAA1 Human)

Expression

Mammalian

Promoter

pCAGGs

Available Since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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pBud-EGFP

Plasmid

#23027

Depositor

Andrea LeBlanc

Article

Jodoin et al PLoS One. 2009 . 4(8):e6647.

Insert

EGFP (eGFP )

Expression

Bacterial and Mammalian

Available Since

May 10, 2010

Availability

Academic Institutions and Nonprofits only

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GFP-ZMYND8

Plasmid

#65401

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

ZMYND8 (ZMYND8 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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TfR-sfGFP-myc tag-SpyCatcher003

Plasmid

#133451

Purpose

Expresses SpyCatcher003 for display at the cell surface of mammalian cells, with superfolder GFP for visualization and myc tag for antibody detection

Depositor

Mark Howarth

Article

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

TfR-sfGFP-myc tag-SpyCatcher003

Tags

GSSGS and myc tag

Expression

Mammalian

Mutation

Contains C20 and A23 mutations that improve plasm…

Promoter

CMV

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pmTurquoise2-C1

Plasmid

#60560

Purpose

Expresses the cyan fluorescent protein variant mTurquoise2, can be used to create fusion proteins

Depositor

Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-UbC

Plasmid

#62806

Purpose

To express genes at high levels in neuronal cells. This UbC promoter is more active in neurons than the promoter in CMV-based vectors.

Depositor

Jae Lee, Pantelis Tsoulfas

Article

Soderblom et al Eneuro. 2015 Mar-Apr;2(2). doi: 10.1523/

Type

Empty backbone

Use

AAV; Eucaryotic

Expression

Mammalian

Promoter

hUbC

Available Since

March 26, 2015

Availability

Academic Institutions and Nonprofits only

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pMitoLOC

Plasmid

#58980

Purpose

Expression of preCOX4-mCherry & preSU9-GFP for colocalisation analysis of yeast mitochondrila

Depositor

Markus Ralser

Article

Vowinckel et al Mitochondrion. 2015 Sep;24:77-86. doi: 1

Inserts

mCherry
GFP

Tags

preCOX4 and preSU9

Expression

Yeast

Promoter

ADH1 and MET17

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Tet1

Plasmid

#60938

Purpose

Expresses Tet1 in mammalian cells

Depositor

Yi Zhang

Article

Wang et al Cell Rep. 2014 Jan 30;6(2):278-84. doi:

Insert

Ten-Eleven Translocation 1 (Tet1 Mouse)

Tags

flag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pmTurquoise2-Mito

Plasmid

#36208

Purpose

In vivo visualization of the mitochondria (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

MTS (COX8A Human)

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-FLEX-jGCaMP8s-WPRE

Plasmid

#162377

Purpose

AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expression

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Has Service

AAV Retrograde, AAV1, and AAV9

Insert

jGCaMP8s

Use

AAV and Cre/Lox

Tags

6xHis

Expression

Mammalian

Mutation

S26M F286Y Q315H

Promoter

Synapsin

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pBS513 EF1alpha-cre

Plasmid

#11918

Purpose

Expresses Cre under the control of the elongation factor-1 alpha (EF1alpha) promoter; includes upstream Kozak modification to increase expression.

Depositor

Brian Sauer

Article

Le et al Anal Biochem. 1999 Jun 1. 270(2):334-6.

Insert

cre (cre bacteriophage P1)

Use

Cre/Lox

Expression

Mammalian

Promoter

EF1alpha

Available Since

March 16, 2007

Availability

Academic Institutions and Nonprofits only

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pGWB506

Plasmid

#74848

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with sGFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
CaV1.3e[8a,11,31b,Δ32,42a]

Plasmid

#49333

Purpose

Repaired Addgene #26576 to agree with the rat ref sequence. Repairs are @: nt 3310 (GTG->GCG) [Val->Ala]; nt 731 (TCA->GGA) [Ser->Gly].

Depositor

Diane Lipscombe

Article

Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.

Insert

CACNA1D (Cacna1d Rat)

Expression

Mammalian

Mutation

Contains exons 8a, 11, 31b and 42a.Deletion of ex…

Promoter

CMV

Available Since

Feb. 14, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pEV53D

Plasmid

#44168

Depositor

John Olsen

Article

Patel et al J Gene Med. 2013 Jan;15(1):51-62. doi: 1

Insert

Equine infectious anemia virus (EIAV) gag-pol,rev, and S2

Use

Lentiviral

Expression

Mammalian

Mutation

A131T mutation in gag; Deletion of viral LTR sequ…

Promoter

CMV

Available Since

April 24, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge

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8,964 results