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We narrowed to 4,062 results for: p2a

Showing: 1771 - 1800 of 4062 results

  3. pCDNA-CRY2-mCh-superPLDx10-P2A-CIBN-CAAX

    Plasmid
    #188991
    Purpose
    A plasmid for dual expression of CRY2-mCh-superPLDx10 (an engineered PLD mutant with 10 times higher activity than the wild-type) and plasma membrane-tagged CIBN
    Depositor
    Jeremy Baskin
    Article
    Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415
    Insert
    PLD
    Use
    Tags
    CAAX, CIBN, CRY2, P2A, and mCherry
    Expression
    Mammalian
    Mutation
    F163L, K249R, G328S, G381V, G429D, D480G
    Promoter
    CMV
    Available since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    188991-plasmid-map-sequence-id-371644

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  12. XLone-Puro SARS-CoV2 N P2A eGFP-NLS

    Plasmid
    #154399
    Purpose
    PiggybacTransposon-based tunable and temporal expression control of SARS-CoV2 N and nuclear eGFP
    Depositor
    Xiaoping Bao
    Insert
    SARS-CoV2 N Protein (N SARS-CoV2)
    Use
    Piggybac
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G
    Available since
    June 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    154399-plasmid-map-sequence-id-304590

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  14. pRRL-SFFV-rtTA3-IRES-Luc-P2A-mtagBFP

    Plasmid
    #176027
    Purpose
    construct expresses the reverse tet-responsive transactivator 3 (rtTA3) together with Luciferase (Luc) for bioluminescence in vivo imaging, and mTaqBFP for enriching transgenic cells by flow cytometry
    Depositor
    Irmela Jeremias
    Article
    Liu et al Biomark Res. 2020 Sep 16;8:46. doi: 10.1
    Inserts
    rtTA3
    Luc2
    mtagBFP
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    176027-plasmid-map-sequence-id-351264

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  15. pSLPB2-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

    Plasmid
    #187945
    Purpose
    dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Edda Schulz
    Article
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Insert
    SpdCas9-tagRFPt-P2A-tagBFP
    Use
    Synthetic Biology; Piggybac
    Tags
    NLS, NLS + HA-tag, P2A, tagBFP, and tagRFPt
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187945-plasmid-map-sequence-id-373705

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  16. pAAV-Dlx5/6-Chronos-eGFP-p2a-nls-mScarlet

    Plasmid
    #121543
    Purpose
    Chronos with red nucleus-targeted fluorescence for inhibitory neurons
    Depositor
    Matthias Prigge
    Insert
    blue-absorbing channelrhodopsin Chronos with eGFP and nls-mScarlet
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    dlx5/6
    Available since
    Feb. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    121543-plasmid-map-sequence-id-235975

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  17. pCMV-T7-ABE8e-SpCas9-HiFi-P2A-EGFP (LM446)

    Plasmid
    #197506
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-HiFi(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-ABE8e-nSpCas9-HiFi-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA and nSpCas9-HiFi(D10A/R69…
    Promoter
    CMV and T7
    Available since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197506-plasmid-map-sequence-id-386645

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  18. pCDNA-CRY2-mCh-superPLDx30-P2A-CIBN-CAAX

    Plasmid
    #188992
    Purpose
    A plasmid for dual expression of CRY2-mCh-superPLDx30 (an engineered PLD mutant with 30 times higher activity than the wild-type) and plasma membrane-tagged CIBN
    Depositor
    Jeremy Baskin
    Article
    Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415
    Insert
    PLD
    Use
    Tags
    CAAX, CIBN, CRY2, P2A, and mCherry
    Expression
    Mammalian
    Mutation
    K109R, P245A, G328S, G381V, G429D
    Promoter
    CMV
    Available since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    188992-plasmid-map-sequence-id-371645

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  23. Lenti-EFS-NTID194-GSQ-PMLiva3MAS-P2A-BLAST

    Plasmid
    #208039
    Purpose
    Enables constitutive expression of N-terminal Split-TurboID-fused PML (isoform IVa; 3MAS mutant; K>R sumoylation sites, mutated SIM); selection with blasticidin
    Depositor
    James Sutherland, Rosa Barrio
    Article
    Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:
    Insert
    PML (isoform IVa; 3MAS) (PML Human)
    Use
    Lentiviral
    Tags
    FLAG, N-terminal Split-TurboID
    Expression
    Mutation
    3MAS mutant: K65R, K160R and K490R; mutated SIM
    Promoter
    EFS
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    208039-plasmid-map-sequence-id-410207

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  24. pAAV-SynI-CreOn-FlpOff-MCS-P2A-EGFP

    Plasmid
    #176276
    Purpose
    Viral vector for co-expression of a CDS of interest and EGFP in cells expressing Cre AND NOT Flp driven by a Synapsin promoter. Contains an in-frame P2A sequence and an MCS for cloning of the CDS.
    Depositor
    Martin Riccomagno
    Article
    Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    MCS-P2A-EGFP
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    human Synapsin I
    Available since
    Dec. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    176276-plasmid-map-sequence-id-350687

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  25. pCDNA-CRY2-mCh-superPLDx50-P2A-CIBN-CAAX

    Plasmid
    #188993
    Purpose
    A plasmid for dual expression of CRY2-mCh-superPLDx50 (an engineered PLD mutant with 50 times higher activity than the wild-type) and plasma membrane-tagged CIBN
    Depositor
    Jeremy Baskin
    Article
    Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415
    Insert
    PLD
    Use
    Tags
    CAAX, CIBN, CRY2, P2A, and mCherry
    Expression
    Mammalian
    Mutation
    D201G, A258T, K327R, G328S, G381V, G429D, T450A
    Promoter
    CMV
    Available since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    188993-plasmid-map-sequence-id-371646

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  28. pHR-mCherry-p2A-LIC1 Y101A, Y103A, W120A

    Plasmid
    #74606
    Purpose
    expresses LIC1 in mammalian cells; HA-tagged; point mutants that abrogate binding to dynein
    Depositor
    Ron Vale
    Article
    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554
    Insert
    full length human dynein light intermediate chain 1 Y101A, Y103A, W120A (DYNC1LI1 Human)
    Use
    Lentiviral
    Tags
    HA tag
    Expression
    Mammalian
    Mutation
    Y101A, Y103A, W120A
    Promoter
    SFFV
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    74606-plasmid-map-sequence-id-151888

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Showing: 1771 - 1800 of 4062 results