165,725 results

1. pcDNA3.1_nV5-AMOTL2

   Plasmid

   #172999

   Purpose

   constitutive expression of V5-tagged AMOTL2

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   V5-AMOTL2 (AMOTL2 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. p6351 MSCV-CMV-CMV-Flag-HA-NSD3

   Plasmid

   #31357

   Depositor

   Peter Howley

   Article

   Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

   Insert

   NSD3 (NSD3 Human)
   

   Tags

   FLAG and HA

   Expression

   Mammalian

   Available Since

   Oct. 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-53-RtcB

   Plasmid

   #51282

   Purpose

   E. coli expression vector for E. coli RtcB RNA ligase

   Depositor

   Jay Hesselberth

   Article

   Peach et al Nucleic Acids Res. 2015 May 22. pii: gkv

   Insert

   E. coli RtcB
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7 RNA Polymerase

   Available Since

   March 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTRIP-SFFV-EGFP-NLS-FLAG-cGAS

   Plasmid

   #127659

   Purpose

   Over-express EGFP-NLS-FLAG-cGAS

   Depositor

   Nicolas Manel

   Article

   Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

   Insert

   cGAS (CGAS Human)
   

   Use

   Lentiviral

   Tags

   EGFP-NLS-Flag

   Available Since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBS-slpGFP

   Plasmid

   #27171

   Depositor

   Michela Lizier

   Article

   Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

   Insert

   surface (S)-layer protein promoter region fused with modified green fluorescent protein 5
   

   Expression

   Bacterial

   Available Since

   Jan. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBBR1_DVK_AE(J23100-BCD2-E0040m-B0015)

   Plasmid

   #197660

   Purpose

   Expresses GFP under the control of J23100 and BCD2

   Depositor

   Eric Young

   Article

   Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

   Insert

   GFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   June 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCLucf

   Plasmid

   #37328

   Depositor

   John Schiller

   Article

   Johnson et al J Virol. 2009 Mar;83(5):2067-74. Epub 20

   Inserts

   Luciferase (luciferase Fly)
   GFP
   

   Expression

   Mammalian

   Promoter

   CMV and SV40

   Available Since

   July 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMW#3

   Plasmid

   #13350

   Depositor

   Marian Walhout

   Article

   Deplancke et al Cell. 2006 Jun 16. 125(6):1193-205.

   Insert

   LacZ reporter
   

   Expression

   Yeast

   Available Since

   Nov. 9, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBabe-GFP-IKBalpha-wt

   Plasmid

   #15263

   Depositor

   William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   IKB alpha (NFKBIA Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   July 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. mito NAD+ biosensor

    Plasmid

    #186791

    Purpose

    NAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag totarget mitochondria

    Depositor

    Michael Cohen

    Article

    Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

    Insert

    mito NAD+ biosensor
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1_nV5-AMOT (p130)

    Plasmid

    #172997

    Purpose

    constitutive expression of V5-tagged AMOT (p130)

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    V5-AMOT (p130) (AMOT Human)
    

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTRE-tdTomato

    Plasmid

    #50798

    Purpose

    Expression of tdTomato under the control of the TRE-promoter

    Depositor

    Connie Cepko

    Article

    Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

    Insert

    TREtight driven tdTomato fluorescent protein
    

    Use

    Mutli-species

    Promoter

    TREtight

    Available Since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET3a aSyn murine

    Plasmid

    #108865

    Purpose

    Expresses murine alpha synuclein

    Depositor

    Gabriele Kaminski Schierle

    Insert

    alpha synuclein (Snca Mouse)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti_CMV_FR-GECO1c

    Plasmid

    #224358

    Purpose

    Expresses red fluorescent calcium sensor FR-GECO1c

    Depositor

    Adam Cohen

    Article

    Hayward et al Cell Calcium. 2024 Dec 5;125:102983. doi

    Insert

    FR-GECO1c
    

    Use

    Lentiviral

    Available Since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMW#2

    Plasmid

    #13349

    Depositor

    Marian Walhout

    Article

    Deplancke et al Cell. 2006 Jun 16. 125(6):1193-205.

    Insert

    HIS3 reporter
    

    Expression

    Yeast

    Available Since

    Nov. 9, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHiUGE TurboID-HA donor (all-in-one) ORF0

    Plasmid

    #200383

    Purpose

    Tagging endogenously expressed proteins with TurboID-HA, C-terminal

    Depositor

    Scott Soderling

    Article

    Gao et al Nat Commun. 2024 Aug 9;15(1):6801. doi:

    Insert

    TurboID (ref: PMID: 30125270, Addgene plasmid # 107169)
    

    Expression

    Mammalian

    Mutation

    NA

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHR-UCOE-EF1a-Zim3-dCas9-P2A-BFP

    Plasmid

    #188777

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    HA-2xNLS and Zim3 KRAB-NLS fusion

    Promoter

    EF1a

    Available Since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX330S-4

    Plasmid

    #58780

    Purpose

    Expresses Cas9 nuclease and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 nuclease
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP-N1-PARP1

    Plasmid

    #211578

    Purpose

    CMV driven PARP1-eGFP expressing construct with IRES-Neomycin selection

    Depositor

    Chris Lord

    Article

    Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

    Insert

    PARP1 (PARP1 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pL6puro-JeT-pmRevErbA-Venus-NLS-Pest

    Plasmid

    #240106

    Purpose

    A lentiviral (yellow) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by synthtic JeT promoter.

    Depositor

    Achim Kramer

    Article

    Gabriel et al bioRxiv 2025.06.30.661926

    Insert

    murine Nr1d1 promoter+Venus-NLS-Pest-Reporter (Nr1d1 Mouse)
    

    Use

    Lentiviral

    Mutation

    added JeT Promoter

    Available Since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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21. pL6puro-pSV40(1)-pmRevErbA-Venus-NLS-Pest

    Plasmid

    #240112

    Purpose

    A lentiviral (yellow) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by SV40 promoter fragment.

    Depositor

    Achim Kramer

    Article

    Gabriel et al bioRxiv 2025.06.30.661926

    Insert

    murine Nr1d1 promoter+Venus-NLS-Pest-Reporter (Nr1d1 Mouse)
    

    Use

    Lentiviral

    Mutation

    added SV40-Enhancer

    Available Since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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22. pGMC00028 (aka pTS0057)

    Plasmid

    #200832

    Purpose

    AAVS1 guide RNA cloned into the lentiSAM puro backbone

    Depositor

    Raj Chari

    Article

    Yang et al Sci Adv. 2024 Feb 16;10(7):eadj2445. doi

    Insert

    AAVS1 guide RNA
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. psgRNAc

    Plasmid

    #114006

    Purpose

    express sgRNA, p15A, CRM

    Depositor

    David Bikard

    Article

    Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

    Insert

    gRNA
    

    Promoter

    BBa_J23119

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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24. DRD2-3xHA/pCDNA3.1

    Plasmid

    #196940

    Purpose

    Plasmid expressing an antigen targeting the D2 dopamine receptor

    Depositor

    James Trimmer

    Insert

    DRD2 (DRD2 Human)
    

    Tags

    3xHA

    Expression

    Mammalian

    Available Since

    Sept. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. kiCAP-AAV-MyoAAV2A

    Plasmid

    #224440

    Purpose

    Rep/Cap plasmid for the production of MyoAAV 2A, a muscle-tropic AAV capsid in mice.

    Depositor

    Pardis Sabeti

    Article

    Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

    Insert

    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    

    Use

    AAV

    Mutation

    RGDQTTL insert between amino acids 588 and 589 of…

    Promoter

    p41

    Available Since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. FLAG-HA-FbxO11-pcDNA3.1-

    Plasmid

    #52501

    Purpose

    expresses human FbxO11 in mammalian cells with FLAG-HA tag at N-terminus

    Depositor

    Adam Antebi

    Article

    Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

    Insert

    FbxO11 (FBXO11 Human)
    

    Tags

    FLAG-HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. AICSDP-45: TTN-mEGFP

    Plasmid

    #114412

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human TTN, via Cas9-excisable CAGGS-mCherry selection cassette

    Depositor

    Allen Institute for Cell Science

    Article

    Roberts et al Stem Cell Reports. 2019 May 14;12(5):114

    Insert

    TTN Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (TTN Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Available Since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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28. pcDNA Flag-RARalpha

    Plasmid

    #35555

    Depositor

    Jonathan Kurie, Harish Srinivas

    Article

    Srinivas et al Mol Cell Biol. 2005 Feb;25(3):1054-69.

    Insert

    RARalpha (RARA Human)
    

    Tags

    Flag and His

    Expression

    Mammalian

    Available Since

    April 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pIW601-KmCRISPR

    Plasmid

    #98907

    Purpose

    K. marxianus CRISPR Plasmid for sgRNA cloning

    Depositor

    Ian Wheeldon

    Article

    Lobs et al Biotechnol Biofuels. 2017 Jun 24;10:164.

    Inserts

    Codon optimized Cas9
    sgRNA expression cassette
    

    Use

    CRISPR and Synthetic Biology

    Tags

    SV40

    Expression

    Yeast

    Available Since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET16better_H10_HRV3C_chitinase

    Plasmid

    #175091

    Purpose

    Expresses B. licheniformis chitinase with an N terminal 10X His tag and HRV-3C cleavage site in E. coli.

    Depositor

    Jay C Dunlap

    Article

    Bartholomai et al Fungal Genet Biol. 2021 Aug 20;156:10361

    Insert

    chitinase
    

    Tags

    10X His, HRV-3C

    Expression

    Bacterial

    Available Since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pJAF205

    Plasmid

    #60407

    Purpose

    Flag-tagged full length GMAP210/Trip11

    Depositor

    Gregory Pazour

    Article

    Follit et al PLoS Genet. 2008 Dec;4(12):e1000315. doi

    Insert

    GMAP210 (Trip11 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

32. hCathepsin S

    Plasmid

    #11251

    Purpose

    Mammalian expression of human Cathepsin S

    Depositor

    Hyeryun Choe

    Article

    Huang et al J Biol Chem. 2006 Feb 10;281(6):3198-203

    Insert

    cathepsin S (CTSS Human)
    

    Tags

    C9 (GTETSQVAPA)

    Expression

    Mammalian

    Available Since

    Aug. 4, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

33. pT22-GluCNZ-6His

    Plasmid

    #213504

    Purpose

    Designed for cytoplasmic E.coli overexpression of glucanase (β-1,3-glycosidase) for gentle yeast spheroblasting that unlike Zymolyase prevents proteolysis of intracellular components.

    Depositor

    John Denu

    Article

    Kuznetsov et al J Biol Chem. 2018 Aug 3;293(31):12271-12

    Insert

    Glucanase (glucan endo-1,3-beta-glucosidase)
    

    Tags

    6His tag

    Expression

    Bacterial

    Mutation

    1-36 Signal Sequence Deletion

    Promoter

    T7

    Available Since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3.1-2xFLAG-2xSTREP (empty vector)

    Plasmid

    #172604

    Purpose

    Empty vector for the cloning of an N-terminal 2xFLAG-2xSTREP-tagged gene of interest, and expression in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Type

    Empty backbone

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. His6_MBP_FMRP_P451S

    Plasmid

    #198693

    Purpose

    Expression of human FMRP protein in E. coli

    Depositor

    Simpson Joseph

    Article

    Edwards et al Sci Rep. 2020 Sep 28;10(1):15858. doi: 1

    Insert

    FMR1 (FMR1 Human)
    

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    changed proline 451 to serine

    Promoter

    T7

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pSpCas9(BB)-2A-Hygro

    Plasmid

    #127763

    Purpose

    Used for expression of sgRNA in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    June 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pEGFP×2-N1

    Plasmid

    #86775

    Purpose

    A mammalian expression vector with double EGFP at C-terminus.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pSmac-GFP

    Plasmid

    #40881

    Depositor

    Douglas Green

    Article

    Tait et al Dev Cell. 2010 May 18;18(5):802-13.

    Insert

    Smac-GFP (DIABLO Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

39. pCDNA3 Flag Jnk1a1

    Plasmid

    #13798

    Depositor

    Roger Davis

    Article

    Derijard et al Cell. 1994 Mar 25. 76(6):1025-37.

    Insert

    Jnk1a1 (MAPK8 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pEC1.2/EC1.1_spCas9_GFP

    Plasmid

    #161933

    Purpose

    CRISPR plasmid with spCas9 expressed under egg cell promoter - GFP seed selection

    Depositor

    Christian Fankhauser

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge