Skip to main content
Addgene
Showing: 3051 - 3100 of 8580 results

  1. USP7 delta C-terminus

    Plasmid
    #234074
    Purpose
    Expresses USP7 mutant in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 1-1080 (USP7 Human)
    Use
    Tags
    6xHis cleavable with thrombin
    Expression
    Bacterial
    Mutation
    E547 mutated to stop codon
    Promoter
    Available since
    March 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    234074-plasmid-map-sequence-id-464915

    Enlarge

  10. pEF-GalNAcT2-mGold

    Plasmid
    #218964
    Purpose
    Expresses full length GalNAcT2-mGold in mammalian cells
    Depositor
    Benjamin Glick
    Insert
    GalNAcT2-mGold (GALNT2 Human)
    Use
    Tags
    mGold
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Sept. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    218964-plasmid-map-sequence-id-445690

    Enlarge

  11. pEF-GalNAcT2-msYFP2

    Plasmid
    #218963
    Purpose
    Expresses full length GalNAcT2-msYFP2 in mammalian cells
    Depositor
    Benjamin Glick
    Insert
    GalNAcT2-msYFP2 (GALNT2 Human)
    Use
    Tags
    msYFP2
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Sept. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    218963-plasmid-map-sequence-id-446179

    Enlarge

  12. pEF-GalNAcT2-msGFP2

    Plasmid
    #218965
    Purpose
    Expresses full length GalNAcT2-msGFP2 mammalian cells
    Depositor
    Benjamin Glick
    Insert
    GalNAcT2-msGFP2 (GALNT2 Human)
    Use
    Tags
    msGFP2
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Sept. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    218965-plasmid-map-sequence-id-446216

    Enlarge

  13. pGBW-m4046638

    Plasmid
    #153941
    Purpose
    Yeast expression plasmid for SARS-CoV-2 helicase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 helicase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pTEF1
    Available since
    June 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153941-plasmid-map-sequence-id-300503

    Enlarge

  14. pGBW-m4046439

    Plasmid
    #153938
    Purpose
    Yeast expression plasmid for SARS-CoV-2 nsp4
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable thrombin;6xHIS
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pTEF1
    Available since
    June 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153938-plasmid-map-sequence-id-300501

    Enlarge

  15. pGBW-m4046301

    Plasmid
    #153925
    Purpose
    Yeast expression plasmid for SARS-CoV-2 nsp10
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp10 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable thrombin;6xHIS
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pTEF1
    Available since
    June 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153925-plasmid-map-sequence-id-300490

    Enlarge

  16. pGBW-m4046274

    Plasmid
    #153927
    Purpose
    Yeast expression plasmid for SARS-CoV-2 nsp6
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp6 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable thrombin;MBP
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pTEF1
    Available since
    June 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153927-plasmid-map-sequence-id-300492

    Enlarge

  17. pGBW-m4046617

    Plasmid
    #153917
    Purpose
    Yeast expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pTEF1
    Available since
    June 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153917-plasmid-map-sequence-id-300485

    Enlarge

  18. pGBW-m4046334

    Plasmid
    #153901
    Purpose
    Yeast expression plasmid for SARS-CoV-2 3'-to-5' exonuclease
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 3'-to-5' exonuclease (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable thrombin;6xHIS
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pTEF1
    Available since
    June 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153901-plasmid-map-sequence-id-299994

    Enlarge

  19. pGBW-m4046590

    Plasmid
    #153902
    Purpose
    Yeast expression plasmid for SARS-CoV-2 nsp7
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp7 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable thrombin;MBP
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pTEF1
    Available since
    June 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153902-plasmid-map-sequence-id-300443

    Enlarge

  20. pGBW-m4046226

    Plasmid
    #153889
    Purpose
    Yeast expression plasmid for SARS-CoV-2 3'-to-5' exonuclease
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 3'-to-5' exonuclease (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable thrombin;StrepII
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pTEF1
    Available since
    June 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153889-plasmid-map-sequence-id-300431

    Enlarge

  21. pGBW-m4046289

    Plasmid
    #153877
    Purpose
    Yeast expression plasmid for SARS-CoV-2 3'-to-5' exonuclease
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 3'-to-5' exonuclease (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable thrombin;MBP
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pTEF1
    Available since
    June 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153877-plasmid-map-sequence-id-300421

    Enlarge

  22. pGBW-m4046234

    Plasmid
    #149053
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 ORF7b
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 ORF7b (ORF7b Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable thrombin;MBP
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pTEF1
    Available since
    May 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149053-plasmid-map-sequence-id-296273

    Enlarge

  23. pGBW-m4046519

    Plasmid
    #145397
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 nsp11
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp11 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable thrombin;3xFLAG
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pTEF1
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145397-plasmid-map-sequence-id-287574

    Enlarge

  24. pGBW-m4046257

    Plasmid
    #145393
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 nsp8
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp8 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable thrombin;3xFLAG
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pTEF1
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145393-plasmid-map-sequence-id-287570

    Enlarge

  25. ELL1 CtoA

    Plasmid
    #127279
    Purpose
    Testing if ELL1 C to A mutation knock out the action on Myc as it does in human
    Depositor
    Christine Milcarek
    Insert
    ELL1 (NM_007924 plus CEYLHNK to AEYLHNK) (ELL Mouse)
    Use
    Tags
    Twin strep-tag
    Expression
    Mammalian
    Mutation
    CEYLHNK to AEYLHNK by Q5 mutagenesis
    Promoter
    EF1a
    Available since
    Nov. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    127279-plasmid-map-sequence-id-249989

    Enlarge

  30. pSBbi-Pur-Lyn-AktAR2-EV-D

    Plasmid
    #125200
    Purpose
    SB-transposon plasmid for stable expression of unphosphorylatable "dead" version of lipid raft-targeted Lyn-AktAR2-EV variant of FRET-based AKT activity reporter
    Depositor
    Eric Davis
    Insert
    Lyn-AktAR2-EV-D
    Use
    Transposon
    Tags
    EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…
    Expression
    Mammalian
    Mutation
    FoxO1 Thr-24 changed to Val rendering this inacti…
    Promoter
    EF1a/RPBSA
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125200-plasmid-map-sequence-id-243591

    Enlarge

  42. pSBBi-RP GST-PcCS

    Plasmid
    #139160
    Purpose
    SB-transposon with constitutive bi-directional promoter. One side contains N-GST Paullinia cupana caffeine synthase; the other side contains RFP and puromycin resistance genes.
    Depositor
    Neal Devaraj
    Insert
    N-GST caffeine Synthase [Paullinia cupana var. sorbilis]
    Use
    Transposon
    Tags
    glutathione transferase
    Expression
    Mammalian
    Mutation
    Promoter
    EF1α
    Available since
    Availability
    Academic Institutions and Nonprofits only
    139160-plasmid-map-sequence-id-309126

    Enlarge

  44. pSBbi-RB

    Plasmid
    #60522
    Purpose
    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and blasticidin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a/RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60522-plasmid-map-sequence-id-97524

    Enlarge

  45. pSBbi-GP

    Plasmid
    #60511
    Purpose
    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: GFP and puromycin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a/RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60511-plasmid-map-sequence-id-222915

    Enlarge

  46. pSBbi-RP

    Plasmid
    #60513
    Purpose
    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and puromycin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a/RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60513-plasmid-map-sequence-id-373029

    Enlarge

Showing: 3051 - 3100 of 8580 results