8,677 results

1. CaV2.1 (pcDNA6)

   Plasmid

   #26578

   Depositor

   Diane Lipscombe

   Article

   Richards et al Eur J Neurosci. 2007 Nov . 26(10):2950-6

   Insert

   Cacna1a (Cacna1a Mouse)
   

   Use

   Tags

   His6 and V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W

   Plasmid

   #67974

   Purpose

   CRISPR gRNA expression vector with an improved scaffold and puro/BFP markers

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette, PGKpuro2ABFP cassette, WPRE
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Deleted BbsI site within WPRE

   Promoter

   human U6 promoter, mouse PGK promoter

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSLQ9834_sgBB: pHR-hU6-CasMINI sgRNA_#2; EF1a-Puro-T2A-BFP- WPRE

   Plasmid

   #180280

   Purpose

   The CasMINI sgRNA cloning backbone with sites 'BsmBI' for inserting new guide sequences.

   Depositor

   Stanley Qi

   Article

   Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

   Insert

   CasMINI sgRNA (backbone) and BFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. mCyRFP1-C1

   Plasmid

   #84355

   Purpose

   Expresses mCyRFP1 fluorescent protein in an empty backbone

   Depositor

   Ryohei Yasuda

   Article

   Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

   Insert

   mCyRFP1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A161K and N41A, reletive to CyRFP1

   Promoter

   CMV

   Available since

   Nov. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pL

   Plasmid

   #196286

   Purpose

   35S promoter-driven expression of the positive-sense TSWV L RNA segment encoding codon-optimized L (RdRp)

   Depositor

   Zhenghe Li

   Article

   Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

   Insert

   Full length TSWV L antigenome encoding codon-optimized L (RdRp)
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   duplicated cauliflower mosaic virus 35S promoter

   Available since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3 p53 WT

   Plasmid

   #69003

   Purpose

   mammalian expression of wild type human p53

   Depositor

   David Meek

   Article

   Loughery et al Nucleic Acids Res. 2014 Jul;42(12):7666-

   Insert

   p53 (TP53 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMGS46 (ARF16-PB1-HA-P2A-OsTIR1)

   Plasmid

   #126580

   Purpose

   A repair construct to express ARF16-PB1-HA and OsTIR1 under the control of the CMV promoter from the human AAVS1 locus

   Depositor

   Michael Guertin

   Article

   Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

   Insert

   OsARF16-PB1 domain
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Flag-FOXA1

   Plasmid

   #153109

   Purpose

   Expression of Flag tagged human FOXA1 in mammalian cells

   Depositor

   Stefan Koch

   Article

   Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

   Insert

   human FOXA1 (FOXA1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE94

   Available since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. mEmerald-CD9-10

   Plasmid

   #54029

   Purpose

   Localization: Tetraspanin/Membrane, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Insert

   CD9 (CD9 Human)
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 2, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pLV-EBFP2-nuc

    Plasmid

    #36085

    Depositor

    Pantelis Tsoulfas

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Replace EYFP of the vector pEYFP-Nuc from Clontec…

    Promoter

    Available since

    June 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-CreERT2

    Plasmid

    #14797

    Purpose

    Mammalian expression of conditionally active form of Cre recombinase (not as tightly regulated as pCAG-ERT2CreERT2)

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

    Insert

    Cre-ERT2 fusion protein
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. spNW50

    Plasmid

    #173486

    Purpose

    Expresses spNW50 in Bl21

    Depositor

    Huan Bao

    Article

    Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

    Insert

    spNW50
    

    Use

    Tags

    Histag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCS-CG

    Plasmid

    #12154

    Depositor

    Inder Verma

    Article

    Miyoshi et al J Virol. 1998 Oct . 72(10):8150-7.

    Insert

    CMV-GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEGFP-NR2A

    Plasmid

    #17924

    Depositor

    Stefano Vicini

    Article

    Luo et al Neuropharmacology. 2002 Mar . 42(3):306-

    Insert

    NMDAR2A (Grin2a Rat)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA4/TO-Flag-H3.3

    Plasmid

    #47980

    Purpose

    tetracycline-inducible expression of human Flag tagged H3F3A

    Depositor

    Bing Zhu

    Article

    Huang et al PLoS Genet. 2013 Jun;9(6):e1003558. doi:

    Insert

    H3F3A (H3-3A Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    288 bp T to C synonymous mutation

    Promoter

    CMV promoter with tetracycline operator

    Available since

    Sept. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pMA7CR_2.0

    Plasmid

    #73950

    Purpose

    pMA7CR_2.0 contains arabinose inducible λ/RED β-protein and dam, and aTc inducible CRISPR/Cas9 and recX

    Depositor

    Alex Nielsen

    Article

    Ronda et al Sci Rep. 2016 Jan 22;6:19452. doi: 10.10

    Inserts

    cas9
    lambda beta
    dam
    recX
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pAra and pTet

    Available since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCEP4-hERG1a

    Plasmid

    #53052

    Purpose

    Expresses alpha subunit of hERG1a K channel in mammalian cells

    Depositor

    Michael Sanguinetti

    Article

    Mitcheson et al Proc Natl Acad Sci U S A. 2000 Oct 24;97

    Insert

    Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. NPC1 His6 EGFP

    Plasmid

    #53521

    Depositor

    Matthew Scott

    Article

    Lopez et al J Neurosci. 2011 Mar 23;31(12):4367-78.

    Insert

    NPC1
    

    Use

    Tags

    EGFP and His6

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti-PalmGRET

    Plasmid

    #158221

    Purpose

    BRET-based reporter to enable pan-extracellular particle labelling ranging from exomeres (< 50 nm) to small (< 200 nm; e.g. exosomes) and medium and large (> 200 nm; e.g. microvesicles) EVs.

    Depositor

    Charles P. Lai

    Article

    Wu et al Adv Sci (Weinh). 2020 Aug 16;7(19):20014

    Insert

    PalmGFP-Nluc
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti CMV/TO GFP-MDC1 (779-2)

    Plasmid

    #26285

    Depositor

    Eric Campeau

    Insert

    Mediator of DNA damage checkpoint 1 (MDC1 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLentiCRISPR-mCherry

    Plasmid

    #75161

    Purpose

    Derived from LentiCRISPR v1 but expresses mCherry instead of puromycin resistance. 3rd generation.

    Depositor

    Beat Bornhauser

    Article

    McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. PH-PLCD1(R40L)-GFP

    Plasmid

    #51408

    Purpose

    Non-PI(4,5)P2 binding control for PH-PLCD1-GFP

    Depositor

    Tamas Balla

    Article

    Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

    Insert

    PH-PLCD1 (aa 1–170) (PLCD1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Arginine 40 mutated to leucine

    Promoter

    Available since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

23. pLV-tTRKRAB

    Plasmid

    #12249

    Depositor

    Didier Trono

    Article

    Wiznerowicz et al J Virol. 2003 Aug . 77(16):8957-61.

    Insert

    TetR-KRAB fusion
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. eGFP STAT1 WT

    Plasmid

    #12301

    Depositor

    Alan Perantoni

    Article

    Timofeeva et al Oncogene. 2006 Jun 26. ():.

    Insert

    STAT1 (STAT1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. LZRS-HOTAIR

    Plasmid

    #26110

    Depositor

    Howard Chang

    Article

    Gupta et al Nature. 2010 Apr 15. 464(7291):1071-6.

    Insert

    HOTAIR (HOTAIR Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pN1/G3BP1-iRFP

    Plasmid

    #129339

    Purpose

    Stress granule marker

    Depositor

    Yasushi Okada

    Article

    Yaginuma et al bioRxiv 2021.10.08.463131

    Insert

    iRFP (from Dr. M. Matsuda)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3.3_OCT4

    Plasmid

    #26816

    Depositor

    Derrick Rossi

    Article

    Warren et al Cell Stem Cell. 2010 Sep 29. ():.

    Insert

    5'UTR-OCT4-3'UTR (POU5F1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 17, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. CDH5-AbR-GFP

    Plasmid

    #122970

    Purpose

    Lenti plasmid containing a CDH5 promoter driving GFP followed by T2A and an antibiotic resistance cassette (zeocin). Used for selection of endothelial cells.

    Depositor

    Budd A. Tucker

    Inserts

    CDH5p
    CopGFP
    Zeocin
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    May 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pL-CRISPR.EFS.GFP

    Plasmid

    #57818

    Purpose

    Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. EFS Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    SpCas9
    Sp sgRNA scaffold
    EFS (EEF1A1 Human)
    P2A-eGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    EFS and hU6

    Available since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLV-Ftractin-mRuby3-p2A-mTurquoise-MLC-IRES-Blast

    Plasmid

    #85146

    Purpose

    lentiviral expression of a stoichiometric F-actin and myosin light chain reporter

    Depositor

    Tobias Meyer

    Article

    Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

    Insert

    Ftractin-mRuby3-P2A-mTurquoise-MLC
    

    Use

    Lentiviral

    Tags

    mRuby3, mTurquoise

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCAS9counter

    Plasmid

    #107192

    Purpose

    Expresses CAS9 and sgRNA for targeted counterselection in S. aureus

    Depositor

    Steve Salipante

    Article

    Penewit et al MBio. 2018 Feb 20;9(1). pii: mBio.00067-

    Inserts

    Cas9
    sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    prab17

    Available since

    Feb. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Sec61b-V5-TurboID

    Plasmid

    #166971

    Purpose

    Expresses ER targeted TurboID for secretome biotinylation

    Depositor

    Hyun-Woo Rhee

    Article

    Kim et al Nat Commun. 2021 Sep 1;12(1):5204. doi:

    Insert

    Sec61b-V5-TurboID
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET32a-HD46Q

    Plasmid

    #11515

    Depositor

    Pamela Bjorkman

    Article

    Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

    Insert

    huntingtin, exon 1, 46Q glutamines (HTT Human)
    

    Use

    Tags

    His, His (out of frame), and Trx

    Expression

    Bacterial

    Mutation

    huntingtin exon 1 with 46 glutamines, vector m…

    Promoter

    Available since

    April 28, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pXR004: CasRx pre-gRNA cloning backbone

    Plasmid

    #109054

    Purpose

    hU6-driven expression of guide RNAs compatible with CasRx. Contains BbsI sites for guide cloning flanked by 5' and 3' full-length DRs

    Depositor

    Patrick Hsu

    Article

    Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pEGFP VAMP7 (1-220)

    Plasmid

    #42316

    Depositor

    Thierry Galli

    Article

    Martinez-Arca et al J Cell Biol. 2000 May 15;149(4):889-900.

    Insert

    VAMP7 (1-220) (VAMP7 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pMJ920

    Plasmid

    #42234

    Depositor

    Jennifer Doudna

    Article

    Jinek et al elife. 2013;2:e00471. doi: 10.7554/eLife

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    GFP, HA epitope, and NLS (nuclear localization si…

    Expression

    Mammalian

    Mutation

    codon-optimized synthetic DNA sequence

    Promoter

    CMV

    Available since

    March 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCMV-SynB

    Plasmid

    #174861

    Purpose

    Expresses mouse syncytin B in mammalian cells

    Depositor

    Feng Zhang

    Article

    Segel et al Science. 2021 Aug 20;373(6557):882-889.

    Insert

    syncytin B (Synb Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCDH-EF1a-eFFly-mCherry

    Plasmid

    #104833

    Purpose

    3rd generation lentiviral vector. eFFly luciferase for in vivo Imaging, mCherry for fluorescent detection

    Depositor

    Irmela Jeremias

    Article

    Ebinger et al Cancer Cell. 2016 Dec 12;30(6):849-862.

    Inserts

    eFFly
    mCherry
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. 2C::tdTomato Reporter

    Plasmid

    #40281

    Depositor

    Samuel Pfaff

    Article

    Macfarlan et al Nature. 2012 Jul 5;487(7405):57-63.

    Insert

    2C Promoter (2-cell stage promoter)
    

    Use

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pBPGAL80Uw-6

    Plasmid

    #26236

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Genetics. 2010 Aug 9. ():.

    Insert

    GAL80 (GAL80 Budding Yeast)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge