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Plasmid#78658PurposeGenerates B2 inserts suitable for rescueDepositorInsertB2rescue cassette
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
PB-Pitx2
Plasmid#60668PurposePiggyBac expression vector encoding murine Pitx2 downstream of a doxycycline inducible promoterDepositorInsertPitx2 (Pitx2 Mouse)
UsePiggybacTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
A3F-Cas9n-UGI
Plasmid#119138PurposeBase editor made from APOBEC3FDepositorInsertAPOBEC3F-Cas9 nickase-UGI
UseCRISPRTagsExpressionMutationNonePromoterCMVAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
A3C-Cas9n-UGI
Plasmid#119136PurposeBase editor made from APOBEC3CDepositorInsertAPOBEC3C-Cas9 nickase-UGI
UseCRISPRTagsExpressionMutationNonePromoterCMVAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFP.R375
Plasmid#138245PurposeExpresses the split mCherry reporter interrupted by the NrdA-2 intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
766_SMAD4 in pmiRGlo
Plasmid#78130Purposeluciferase reporter for miRNA activityDepositorInsertSMAD4 (SMAD4 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutation3'UTR containing miRNA binding sitesPromoterPGKAvailable sinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHJ3: LexA-VHH-Control
Plasmid#108241PurposeLexA DNA binding domain fusing with anti-RNase A VHH antibodyDepositorInsertLexA DNA binding domain fusing with anti-RNase A VHH antibody
UseSynthetic BiologyTagsExpressionMutationPromoterpLacO1Available sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
A3G-Cas9n-UGI
Plasmid#119139PurposeBase editor made from APOBEC3GDepositorInsertAPOBEC3G-Cas9 nickase-UGI
UseCRISPRTagsExpressionMutationNonePromoterCMVAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-G-GECO1.1
Plasmid#32467DepositorInsertG-GECO1.1
UseLab constructedTags6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…ExpressionBacterialMutationGCaMP3 K69E/N77Y/D86G/K119I/L173Q/K380N/S404G/E43…PromoterAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMC3-yeGFP
Plasmid#176181Purposeyeast MoClo level-0 part plasmid type 3 containing yeast-enhanced GFPDepositorInsertyEGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pL59-Nat
Plasmid#140465PurposeCRISPR/Cas9 engineering with nourseothricin selectionDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterial and YeastMutationPromoterAvailable sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pILGFP3A2 (2)
Plasmid#185852PurposeTesting the TEF1 promoter appended with 3 tetracycline riboswitch using a green fluorescence protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1-PTEF1>3*TcRb>yEGFP>TPGK1-TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSopBC460S
Plasmid#112514PurposePsopB driven sopB (catalytically inactive) and sigEDepositorInsertsopB (catalytically inactive)/sigE operon
UseTagsExpressionBacterialMutationSopB C460SPromoterPsopBAvailable sinceJuly 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR-MCS-YFP-NLS
Plasmid#145274PurposeMammalian Expression of Nuclear YFP with N-terminal MCS and SV40 NLSDepositorInsertMCS and YFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBM13-sd4U
Plasmid#179691PurposeBacterial expression of base editorDepositorInsert[sd4U] UGI-TALE3-DddA-G1397-N (Q1310R + S1330I + T1380I)
UseTagsExpressionBacterialMutationQ1310R, S1330I, T1380IPromoterAvailable sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMBP_hBrg1 (J1 antibody)
Plasmid#26109DepositorInsertBrg1 (SMARCA4 Human)
UseTagsMBPExpressionBacterialMutationPromoterAvailable sinceSept. 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
pHJ18
Plasmid#48357PurposeTargets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Puromycin//Gancyclovir selectableDepositorInsertPartial beta-TUB followed by 5' ALD UTR-loxP-SAS-PUR-Ty1-TK-loxP-3' ALD UTR
UseCre/Lox; Knockout in t. bruceiTagsExpressionMutationPromoterAvailable sinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
P199
Plasmid#139717PurposeModule plasmid, individual component of Fungal Bioluminescent Pathway, Luz, Luz promoter is circadian controlledDepositorInsertpMOD_D_pAtELF4:NnLuz:trbcSE9
UseLuciferaseTagsExpressionPlantMutationPromoterAtELF4Available sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
MAGE-A12Pro pGL4basic
Plasmid#78139Purposeluciferase reporter plasmidDepositorInsertMAGE-A12 (MAGEA12 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationcontains -425/+115 of promoterPromoterMAGE-A12Available sinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCNL-N1_PTS2
Plasmid#65676PurposeExpresses PTS2-CNL in mammalian cellsDepositorInsertperoxisomal targeting signal 2
UseLuciferaseTagsCNLExpressionMammalianMutationPromoterCMVAvailable sinceJune 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFP.R271
Plasmid#138220PurposeExpresses the split mCherry reporter interrupted by the SspDnaX artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pILGFP9E3
Plasmid#185842PurposeTesting the TEF1 promoter inserted with 4 TetO elements using a green fluorescence protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1-PTEF1+[TetO]>yEGFP>TPGK1-TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFUSE-B_P2rP4
Plasmid#97185PurposeFor fusion gene cloning. Recombine Gene B into this Gateway Entry VectorDepositorTypeEmpty backboneUseGatewayTagsExpressionMutationPromoterAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
BII-ChBtW-PLAGL2
Plasmid#133369PurposePiggybac vector for constitutive expressionDepositorInsertPLAGL2 (PLAGL2 Human)
UseTagsFlagExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS42K_PCP-VP64_B-PP7
Plasmid#177157PurposeExpresses transactivator (PCP-VP64) and scRNA (B-PP7) in yeast cellsDepositorInsertsPCP-VP64
B-PP7
UseTagsSV40 NLSExpressionYeastMutationPromoterADH1 promoter and SNR52 promoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYNL-C1_H2B
Plasmid#65702PurposeExpresses YNL-H2B in mammalian cellsDepositorInserthistone H2B
UseLuciferaseTagsYNLExpressionMammalianMutationPromoterCMVAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHJ41
Plasmid#48360PurposeTargets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Blasticidin/Gancyclovir selectableDepositorInsertPartial beta-TUB followed by 5' ALD UTR-loxP-SAS-BSD-Ty1-TK-loxP-3' ALD UTR
UseCre/Lox; Knockout in t. bruceiTagsExpressionMutationPromoterAvailable sinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
P315-FBP_10
Plasmid#139701PurposeT-DNA encoding Fungal Bioluminescent Pathway Components, control pathway, missing CPH and NpgaDepositorInsertpUBQ1:NnH3H:tNos, pCmYLCV:NnHisps:tHsp, pAtuMas:NnLuz:trbcSE9
UseLuciferaseTagsExpressionPlantMutationPromotervariousAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMC8b-XI3up
Plasmid#176170Purposeyeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XI-3DepositorInsert5' homology for integration site XI-3
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC7-XI3dw
Plasmid#176171Purposeyeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XI-3DepositorInsert3' homology for integration site XI-3
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC8b-XI5up
Plasmid#176172Purposeyeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XI-5DepositorInsert5' homology for integration site XI-5
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC7-XI5dw
Plasmid#176173Purposeyeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XI-5DepositorInsert3' homology for integration site XI-5
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC8b-XII5up
Plasmid#176176Purposeyeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XII-5DepositorInsert5' homology for integration site XII-5
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC7-XII5dw
Plasmid#176177Purposeyeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XII-5DepositorInsert3' homology for integration site XII-5
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC8b-X2up
Plasmid#176162Purposeyeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site X-2DepositorInsert5' homology for integration site X-2
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC7-X2dw
Plasmid#176163Purposeyeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site X-2DepositorInsert3' homology for integration site X-2
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEPOR2KN0040
Plasmid#117607PurposeLevel2 MoClo construct, containing level1 plant expression cassettes for SaCas9(pEPOR1CB0015) and sgRNA_NbPDS(pEPOR1CB0058)DepositorInsert[35S:SaCas9(pEPOR1CB0015) ] +[AtU6-26:sgRNA]
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pEPOR2KN0038
Plasmid#117605PurposeLevel2 MoClo construct, containing level1 plant expression cassettes for LbCas12a(pEPOR1CB0013) and sgRNA_AtADH1 {AT1G77120}(pEPOR1CB0029)DepositorInsert[35S:LbCas12a(pEPOR1CB0013) ] +[AtU6-26:sgRNA]
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUAP1_Ta_Yr7_CDS_no_STOP
Plasmid#141093PurposeLevel 0 Golden gate module with the coding sequence of Cadenza-Yr7 without its STOP codonDepositorInsertTa_Yr7
UseUnspecified; Level 0 module for golden gate cloni…TagsExpressionMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R365
Plasmid#138235PurposeExpresses the split mCherry reporter interrupted by the EP-Pri artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
MYMH 464
Plasmid#138068PurposeNatural Terminator ECK120033736 inserted between mCherry and GFPDepositorInsertJ23104-mCherry-33736-GFP-pSEVA-331Bb
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pONL-N1_LAMP1
Plasmid#65686PurposeExpresses LAMP1-ONL in mammalian cellsDepositorInsertLAMP1
UseLuciferaseTagsONLExpressionMammalianMutationPromoterCMVAvailable sinceJan. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
JH05
Plasmid#73516PurposemYPet_PH pCFJ150 - contain C. elegans codon optimized mYPetDepositorInsertpmex-5::mPYet::PH::tbb-2
UseTagsmYPetExpressionMutationPromotermex-5Available sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
miR190 in pcDNA3.1
Plasmid#78122PurposeMammalian expression of miR190DepositorInsertmiR190 (MIR190A Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
A102-GAP-BoCCS-GRM
Plasmid#123286PurposeExpression of Glo3 ΔAmpH (1-459) in E.ColiDepositorInsertADP-ribosylation factor GTPase-activating protein (GLO3 Budding Yeast)
UseTagsHis and MBPExpressionBacterialMutationPromoterT5Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEPOR2KN0039
Plasmid#117606PurposeLevel2 MoClo construct, containing level1 plant expression cassettes for AsCas12a(pEPOR1CB0014) and sgRNA_AtADH1 {AT1G77120}(pEPOR1CB0028)DepositorInsert[35S:AsCas12a(pEPOR1CB0014) ] +[AtU6-26:sgRNA]
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pONL-C1_H2B
Plasmid#65704PurposeExpresses ONL-H2B in mammalian cellsDepositorInserthistone H2B
UseLuciferaseTagsONLExpressionMammalianMutationPromoterCMVAvailable sinceJune 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET11a-TAX-L10-STEPtag
Plasmid#159375PurposeExpresses STEPtag fused to the C-terminus of inactive T. aurantiacus xylanase 10A, as a mock protein of interest.DepositorInsertTAX-L10-STEPtag
UseTags6x His tagExpressionBacterialMutationPromoterT7Available sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET11a-STEPtag-L10-TAX
Plasmid#159374PurposeExpresses STEPtag fused to the N-terminus of inactive T. aurantiacus xylanase 10A, as a mock protein of interest.DepositorInsertSTEPtag-L10-TAX
UseTags6x His tagExpressionBacterialMutationPromoterT7Available sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only