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mCherry

Plasmid

#176016

Purpose

Mammalian expression of mCherry. Parton lab clone DFQ

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

mCherry

Expression

Mammalian

Available Since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pmCTIP2-N106

Plasmid

#66808

Purpose

2nd generation lentiviral transfer plasmid. Expresses mouse CTIP2 with blasticidin resistance

Depositor

Andrew Yoo

Article

Richner et al Nat Protoc. 2015 Oct;10(10):1543-55. doi

Insert

CTIP2 isoform b (Bcl11b Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

Human EF1a

Available Since

Sept. 21, 2015

Availability

Academic Institutions and Nonprofits only

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Jag1-HA pIRES

Plasmid

#17336

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

Insert

Jagged1 (Jag1 Rat)

Tags

HA X3

Expression

Mammalian

Mutation

A silent C->G mutation at 3237

Available Since

April 24, 2008

Availability

Academic Institutions and Nonprofits only

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pCW57-CMV-ssRFP-GFP-KDEL

Plasmid

#128257

Purpose

Fluorescence reporter to monitor ER-phagy activity

Depositor

Noboru Mizushima

Article

Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

Insert

ssRFPGFPKDEL

Use

Lentiviral

Tags

RFPGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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Cbh_v5 AAV-ABE N-terminal

Plasmid

#137177

Purpose

AAV genome: expresses the N-terminal of v5 AAV-ABE from the Cbh promoter

Depositor

David Liu

Article

Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

Insert

v5 AAV-ABE N-terminal

Use

AAV

Mutation

Cas9 D10A

Promoter

Cbh

Available Since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

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AAV SYN flex PSAM4 GlyR IRES EGFP

Plasmid

#119741

Purpose

Cre-dependent chemogenetic inhibitor expression

Depositor

Scott Sternson

Article

Magnus et al Science. 2019 Mar 14. pii: science.aav52

Has Service

AAV5 and AAV9

Insert

PSAM4 GlyR IRES eGFP (CHRNA7 Synthetic, Human)

Use

AAV

Expression

Mammalian

Mutation

L131G, Q139L, Y217F

Promoter

synapsin

Available Since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV_BE4max

Plasmid

#112093

Purpose

C:G-to-T:A base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

Insert

BE4max

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Floxed-STOP mCherry

Plasmid

#122963

Purpose

mCherry-based fluorescent reporter for Cre-loxP DNA recombination reaction

Depositor

Moritoshi Sato

Article

Kawano et al Nat Chem Biol. 2016 Dec;12(12):1059-1064

Insert

pcDNA3.1_Floxed-STOP mCherry

Use

Cre/Lox

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pHR-scFv-GCN4-sfGFP-GB1-dWPRE

Plasmid

#60907

Purpose

The plasmid encodes an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to sfGFP for imaging

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

scFv-GCN4

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only

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Anti-6xHis [N144/14R]

Plasmid

#177500

Purpose

Mammalian Expression Plasmid of anti-6xHis. Derived from hybridoma N144/14.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-6xHis recombinant mouse monoclonal antibody

Expression

Mammalian

Promoter

Dual CMV

Available Since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-tdPP7-3xmCherry

Plasmid

#74926

Purpose

Expresses tdPP7-3xmCherry

Depositor

Marvin Tanenbaum

Article

Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

Insert

PP7-3xmCherry

Use

Lentiviral

Expression

Mammalian

Available Since

April 29, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-PEmax-P2A-BSD

Plasmid

#174821

Purpose

Mammalian expression of SpCas9 PEmax prime editor with P2A-BSD marker

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

PEmax-P2A-BSD

Tags

P2A-BSD, SV40 bpNLS, and c-Myc NLS

Expression

Mammalian

Mutation

Detailed in manuscript

Promoter

CMV

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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mito-V5-APEX2

Plasmid

#72480

Purpose

Expresses APEX2 in mitochondria

Depositor

Alice Ting

Article

Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

Insert

APEX2

Tags

V5

Expression

Mammalian

Promoter

CMV promoter

Available Since

April 29, 2016

Availability

Academic Institutions and Nonprofits only

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mCherry-VAPB

Plasmid

#108126

Purpose

ER marker

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

mCherry:VAPB (VAPB Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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pMK411 (OsTIR1(F74G) mAID-EGFP-Nluc)

Plasmid

#140659

Purpose

OsTIR1(F74G)-P2A-mAID-EGFP-Nluc

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

OsTIR1(F74G) mAID-EGFP-Nluc TOL2

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-sfGFP 150TAG

Plasmid

#85493

Purpose

pET28a expressing E. coli codon optimized superfolder GFP with TAG stop codon at position 150

Depositor

Ryan Mehl

Article

Peeler et al Methods Mol Biol. 2012;794:125-34. doi:

Insert

superfolder GFP 150TAG

Expression

Bacterial

Mutation

150 TAG (amino acid 150 mutated to stop codon)

Promoter

T7

Available Since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pBAD33.1

Plasmid

#36267

Depositor

Christian Raetz

Article

Chung et al Biochemistry. 2010 May 18;49(19):4126-37

Type

Empty backbone

Expression

Bacterial

Promoter

PBAD

Available Since

Oct. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-Chronos-GFP

Plasmid

#59170

Purpose

AAV-mediated expression of Chronos-GFP under the Syn promoter

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Has Service

AAV Retrograde, AAV1, and AAV5

Insert

Chronos-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

Syn

Available Since

Aug. 13, 2014

Availability

Academic Institutions and Nonprofits only

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p37-2iDMD-LR

Plasmid

#88892

Purpose

37°C full-length DMD luc-red plasmid. Co-expresses human dystrophin, firefly luciferase and mCherry in mammalian cells. Plasmid carries phiC31 and Bxb1 attB sites and can be grown at 37°C.

Depositor

Michele Calos

Article

Farruggio et al Biotechnol J. 2017 Jan 31. doi: 10.1002/

Inserts

Dystrophin (DMD Human)
luciferase
mCherry

Expression

Mammalian

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-PTEN

Plasmid

#28298

Depositor

Maria-Magdalena Georgescu

Article

Takahashi et al EMBO J. 2006 Feb 22. 25(4):910-20.

Insert

PTEN (PTEN Human)

Tags

FLAG

Expression

Mammalian

Available Since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only

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