We narrowed to 3,985 results for: LYC

1. tRNA-gRNA position [D1_2] (GB1205)

   Plasmid

   #75406

   Purpose

   tRNA and scaffold for the assembly of GBoligomers for the first position (positon [D1_2]) of a polycistronic tRNA-gRNA regulated by the dicot U6-26 or U6-1 promoter (3-part multiplexing)

   Depositor

   Diego Orzaez

   Article

   Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

   Insert

   tRNA-gRNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. B-eSpCas9

   Plasmid

   #126761

   Purpose

   Expression plasmid for human codon-optimized increased fidelity Blackjack-eSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than eSpCas9.

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   B-eSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   K848A; K1003A; R1060A; amino acids 1005-1013 repl…

   Promoter

   CBh

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. B-evoSpCas9

   Plasmid

   #126765

   Purpose

   Expression plasmid for human codon-opt. increased fidelity Blackjack-evoSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than evoSpCas9.

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   B-evoSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   M495V; Y515N; K526E; R661Q; amino acids 1005-1013…

   Promoter

   CBh

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pECE beta1 R760A

   Plasmid

   #50511

   Depositor

   Kenneth Yamada

   Article

   Pankov et al J Biol Chem. 2003 May 16;278(20):18671-8

   Insert

   integrin beta1 (ITGB1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R760A

   Promoter

   SV40

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

5. RON6-bio

   Plasmid

   #47785

   Purpose

   Expresses enzymatically monobiotinylated full-length RON6 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

   Depositor

   Gavin Wright

   Article

   Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

   Insert

   Codon-optimised RON6
   

   Use

   Tags

   enzymatic biotinylation sequence and rat Cd4 d3+4

   Expression

   Mammalian

   Mutation

   Exogenous signal peptide of mouse origin replaced…

   Promoter

   CMV

   Available since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR-2B-Nkx2-5 delta HD-G8

   Plasmid

   #98614

   Purpose

   Gateway entry vector NKX2-5 _HD

   Depositor

   Richard Harvey

   Article

   Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

   Insert

   NKX2-5 _HD (Nkx2-5 Mouse)
   

   Use

   Entry vector

   Tags

   Expression

   Mutation

   Deletion of the homeodomain. Replaced by 8 glycin…

   Promoter

   Available since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pNUT N6His Y95F/Y188F hTFNG

   Plasmid

   #69601

   Purpose

   Expresses N-His tagged nonglycosylated human serum transferrin unable to bind iron in the N-lobe

   Depositor

   Ross MacGillivray, Anne Mason

   Article

   Mason et al Protein Expr Purif. 2004 Aug;36(2):318-2

   Insert

   mutated human serum transferrin (TF Human)
   

   Use

   Tags

   Hexa His tag and N-terminal signal peptide, 4 aa …

   Expression

   Mammalian

   Mutation

   Asn413 Asp, Asn611Asp, Tyr95Phe, Tyr188Phe

   Promoter

   SV40

   Available since

   Nov. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. FUS LC 4QQ->GG

   Plasmid

   #226601

   Purpose

   4 glutamine pairs to glycine pairs at positions 27, 28, 92, 93, 132, 133, 157, 158

   Depositor

   Nicolas Fawzi

   Article

   Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

   Insert

   FUS LC 4QQ->GG (FUS Human)
   

   Use

   Tags

   6His

   Expression

   Bacterial

   Mutation

   4 QQ pairs replaced with GG pairs at positions 27…

   Promoter

   T7

   Available since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. FLAG-GRP78-G227D

   Plasmid

   #235207

   Purpose

   Expresses GRP78 with G227D mutation in mammalian cells

   Depositor

   Amy Shiu Lee

   Article

   Tsai et al J Biol Chem. 2015 Mar 27;290(13):8049-64

   Insert

   78-kDa glucose-regulated protein (HSPA5 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Change Glycine 227 to Aspartic Acid

   Promoter

   Available since

   June 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1-SARS-CoV-2-S-ectodomain

    Plasmid

    #235984

    Purpose

    Internally tagged SARS-CoV-2 spike ecto-domain

    Depositor

    Syed Saif Hasan

    Article

    Singh et al J Struct Biol X. 2025 Feb 11;11:100123.

    Insert

    Spike protein (S SARS-CoV-2)
    

    Use

    Tags

    8xHis and Twin-Strep tag and internal Twin-Strep …

    Expression

    Mammalian

    Mutation

    aa 1-1208, D614G, RRAR684-684GSAS, and DK987-987P…

    Promoter

    CMV

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGEX-4T1_GST-LC3C

    Plasmid

    #223727

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

    Insert

    LC3C (MAP1LC3C Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 2-126 only, with Glycine exposed

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Shim_GlyRS_87.19_SHI04113_TP_GFP_pK7fWG2_IVD_FP611

    Plasmid

    #228377

    Purpose

    Expresses N-term transit peptide from S. himalayana Glycine aminoacyl-tRNA synthetase evm.model.fragScaff scaffold 87.19 (SHI04113RA); determination of Sapria himalayana GlyRS target localization

    Depositor

    Daniel Sloan

    Article

    DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

    Insert

    N-terminal transit peptide from S. himalayana GlyRS evm.model.fragScaff scaffold 87.19 (SHI04113RA)
    

    Use

    Tags

    Green Florescent Protien

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pdnCas3-CDA1

    Plasmid

    #229535

    Purpose

    pMV_hyg encoding dnCas3(H74A+D75A)-CDA1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning

    Depositor

    Kevin Verstrepen

    Article

    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

    Inserts

    dnCas3
    Cytidine deaminase
    Uracil glycosylase inhibitor
    

    Use

    Tags

    XTEN

    Expression

    Bacterial and Yeast

    Mutation

    codon optimized for S.cerevisiae and codon optimi…

    Promoter

    Available since

    Jan. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pdnCas3-APOBEC1

    Plasmid

    #229537

    Purpose

    pMV_hyg encoding dnCas3(H74A+D75A)-rAPOBEC1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning

    Depositor

    Kevin Verstrepen

    Article

    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

    Inserts

    rAPOBEC1
    dnCas3
    Uracil glycosylase inhibitor
    

    Use

    Tags

    XTEN

    Expression

    Bacterial and Yeast

    Mutation

    codon optimized for S.cerevisiae and codon optimi…

    Promoter

    Available since

    Jan. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1-F-box:gs10:FRB:HA

    Plasmid

    #228531

    Purpose

    F-box domain of human Skp2 protein in fusion with FRB domain through a flexible glycine-serine linker.

    Depositor

    Roman Jerala

    Article

    Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80

    Insert

    F-box:gs10:FRB:HA (SKP2 Human, Synthetic)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1-myc:Rbx1:gs10:FRB:HA

    Plasmid

    #228534

    Purpose

    Human Rbx1 protein in fusion with FRB domain through a flexible glycine-serine linker.

    Depositor

    Roman Jerala

    Article

    Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80

    Insert

    myc:Rbx1:gs10:FRB:HA (RBX1 Human, Synthetic)
    

    Use

    Tags

    HA and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSuper DGK zeta human

    Plasmid

    #224575

    Purpose

    To downregulate the expression of human DGK zeta in human cells

    Depositor

    Isabel Mérida

    Article

    Avila-Flores et al Immunol Cell Biol. 2017 Jul;95(6):549-56

    Insert

    sh RNAi Diacylglycerol kinase zeta human (DGKZ Human)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    H1

    Available since

    Sept. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSuper Retro GFP DGK alfa mouse

    Plasmid

    #224576

    Purpose

    Negative Control for downregulation of the expression of human DGK alfa in human cells

    Depositor

    Isabel Mérida

    Article

    Avila-Flores et al Immunol Cell Biol. 2017 Jul;95(6):549-56

    Insert

    sh RNAi Diacylglycerol kinase alfa mouse (Dgka Mouse)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mutation

    Promoter

    H1

    Available since

    Sept. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SS-Myc-HexaPro-Foldon (MTK 3a) (pZYW077)

    Plasmid

    #194233

    Purpose

    Mammalian Toolkit part 3a encoding Jason McClellan lab's 6-proline Spike extracellular domain

    Depositor

    Hana El-Samad

    Article

    Weinberg et al bioRxiv 2021.04.20.440678

    Insert

    CD8a Signal Sequence-Myc Tag-SARS-CoV-2 Spike1-1258 (S Synthetic)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMBS-852

    Plasmid

    #218010

    Purpose

    Receptor binding domain of SARS-CoV-2

    Depositor

    Michael Schroda

    Article

    Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

    Insert

    RBD (S SARS-CoV-2)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMBS-850

    Plasmid

    #218005

    Purpose

    Receptor binding domain of SARS-CoV-2

    Depositor

    Michael Schroda

    Article

    Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

    Insert

    RBD (S SARS-CoV-2)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMBS-857

    Plasmid

    #218007

    Purpose

    Receptor binding domain of SARS-CoV-2

    Depositor

    Michael Schroda

    Article

    Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

    Insert

    RBD (S SARS-CoV-2)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pTFG027_Spike_sACE2-resistant_D614G_F486S_3xFlag

    Plasmid

    #191572

    Purpose

    Mutated SARS-CoV-2 Spike under CMV with D614G, C-term 19 amino acid truncation, and 3x FLAG. Encodes F486S mutation. For expression and pseudotyping lentiviral particles.

    Depositor

    Joshua Leonard

    Article

    Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10

    Insert

    SARS-CoV-2 Spike Protein (S Synthetic)
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    D614G and F486S (confers reduced binding affinity…

    Promoter

    CMV

    Available since

    Aug. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. L2088-Mut-GPA-NLuc-Biosensor-Retrovirus-TD138

    Plasmid

    #222876

    Purpose

    Retroviral vector encoding biosensor chains to detect rapamycin (FRB/FKBP domains) and respond with split NanoLuciferase reconstitution (11S/114 fragment). Mixed WT/V84R Glycophorin A (GPA) scaffold.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FRB-GPA(V84R)-NanoLuc114-T2A-FKBP-GPA(WT)-NanoLuc11S
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    FRB chain: V84R in GPA

    Promoter

    MSCV LTR

    Available since

    Aug. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. SSH-pcDNA3.1-SSH-SARS-CoV-2-S-Thr1273Glu

    Plasmid

    #218461

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 spike (1273 residues; Thr1273Glu substitution)

    Depositor

    Syed Saif Hasan

    Article

    Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

    Insert

    SARS-CoV-2 spike protein (1273 residues) carrying Thr1273Glu substitution (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Thr1273AGlu substitution

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. SSH-pcDNA3.1-SSH-SARS-CoV-2-S-Thr1273Asp

    Plasmid

    #218462

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 spike (1273 residues; Thr1273Asp substitution)

    Depositor

    Syed Saif Hasan

    Article

    Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

    Insert

    SARS-CoV-2 spike protein (1273 residues) carrying Thr1273Asp substitution (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Thr1273Asp substitution

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pOPINVHH_H6_His

    Plasmid

    #201719

    Purpose

    Expression of nanobody

    Depositor

    Ray Owens

    Article

    Cornish et al Open Biol. 2024 Jun;14(6):230252. doi: 1

    Insert

    VHH_H6 (S )
    

    Use

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAdEasy-hALDOA D34S

    Plasmid

    #210813

    Purpose

    To reduce the catalytic constant (kcat) for conversion of FBP to dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (G3P)

    Depositor

    Sheng-cai Lin

    Article

    Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

    Insert

    ALDOA (ALDOA Human)
    

    Use

    Adenoviral

    Tags

    HA

    Expression

    Mutation

    residues 34 of ALDOA mutated to serine

    Promoter

    CMV

    Available since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBoBi-hALDOA D34S

    Plasmid

    #210807

    Purpose

    To reduce the catalytic constant (kcat) for conversion of FBP to dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (G3P)

    Depositor

    Sheng-cai Lin

    Article

    Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

    Insert

    ALDOA (ALDOA Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    residues 34 of ALDOA mutated to serine

    Promoter

    CMV

    Available since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. B-HeFSpCas9-A695Q

    Plasmid

    #207368

    Purpose

    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-HeFSpCas9-A695Q
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    N497A, R661A, K848A, Q926A, K1003A, R1060A, amino…

    Promoter

    CBh

    Available since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. PET-B-HypaR-SpCas9-NLS-6xHis

    Plasmid

    #207388

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-HypaR-SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    R661A, N692A, M694A, Q695A, H698A, amino acids 10…

    Promoter

    T7

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. B-HeFSpCas9-A926Q

    Plasmid

    #207370

    Purpose

    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-HeFSpCas9-A926Q
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, K848A, K1003A, R1060A, amino…

    Promoter

    CBh

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. B-evoSpCas9-E526K

    Plasmid

    #207365

    Purpose

    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-evoSpCas9-E526K
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    M495V, Y515N, R661Q, amino acids 1005-1013 replac…

    Promoter

    CBh

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. B-evoSpCas9-V495M

    Plasmid

    #207363

    Purpose

    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-evoSpCas9-V495M
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Y515N, K526E, R661Q, amino acids 1005-1013 replac…

    Promoter

    CBh

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. B-HeFSpCas9-A1060R

    Plasmid

    #207372

    Purpose

    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-HeFSpCas9-A1060R
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, K848A, Q926A, K1003A, amino …

    Promoter

    CBh

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. B-HeFSpCas9-A1003K

    Plasmid

    #207371

    Purpose

    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-HeFSpCas9-A1003K
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, K848A, Q926A, R1060A, amino …

    Promoter

    CBh

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. B-HeFSpCas9-A848K

    Plasmid

    #207369

    Purpose

    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-HeFSpCas9-A848K
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, Q926A, K1003A, R1060A, amino…

    Promoter

    CBh

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. B-HeFSpCas9-A661R

    Plasmid

    #207367

    Purpose

    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-HeFSpCas9-A661R
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    N497A, Q695A, K848A, Q926A, K1003A, R1060A, amino…

    Promoter

    CBh

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. B-evoSpCas9-Q661R

    Plasmid

    #207366

    Purpose

    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-evoSpCas9-Q661R
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    M495V, Y515N, K526E, amino acids 1005-1013 replac…

    Promoter

    CBh

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. B-evoSpCas9-N515Y

    Plasmid

    #207364

    Purpose

    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-evoSpCas9-N515Y
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    M495V, K526E, R661Q, amino acids 1005-1013 replac…

    Promoter

    CBh

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. PET-B-HeFSpCas9-NLS-6xHis

    Plasmid

    #207392

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-HeFSpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A…

    Promoter

    T7

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. 3020_pETcon-SARS-CoV-2-RBD_N501Y

    Plasmid

    #184405

    Purpose

    yeast surface display of the SARS-CoV-2 Alpha variant RBD

    Depositor

    Jesse Bloom

    Article

    Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

    Insert

    SARS-CoV-2 Alpha Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
    

    Use

    Tags

    HA and c-Myc

    Expression

    Yeast

    Mutation

    N501Y

    Promoter

    Available since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pDON223 Spike L241-L242-A243-

    Plasmid

    #180070

    Purpose

    Sars-CoV-2 protein mutation

    Depositor

    Markku Varjosalo

    Article

    Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

    Insert

    SARS-CoV-2 Spike L241-L242-A243- (S SARS-CoV-2)
    

    Use

    Tags

    MAC-tag

    Expression

    Mammalian

    Mutation

    Spike L241-L242-A243-

    Promoter

    Available since

    Jan. 13, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

44. pSUMO R106L-CPTP

    Plasmid

    #170737

    Purpose

    The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

    Depositor

    Rhoderick Brown

    Article

    Simanshu et al Nature. 2013 Aug 22;500(7463):463-7. doi

    Insert

    R106L-CPTP (CPTP Human)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    R106L

    Promoter

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pSUMO K60A-CPTP

    Plasmid

    #170736

    Purpose

    The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

    Depositor

    Rhoderick Brown

    Article

    Simanshu et al Nature. 2013 Aug 22;500(7463):463-7. doi

    Insert

    K60A-CPTP (CPTP Human)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    K60A

    Promoter

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pFastBac1_GI.1_G131C-N172C

    Plasmid

    #162586

    Purpose

    Expresses norovirus GI.1 VP1 protein with mutations G131C-N172C in Sf9 insect cells

    Depositor

    Peter Kwong

    Article

    Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

    Insert

    VP1 protein from human norovirus GI.1
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Glycine 131 changed to Cysteine and Asparagine 17…

    Promoter

    polyhedrin

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. S2 1058 AIDx no UGI

    Plasmid

    #135349

    Purpose

    Expression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 1058 of nSpyCas9 lacking uracil DNA glycosylase inhibitor.

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    nSpyCas9-hAIDx-ID-1058
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. S2 1058 APOBEC3 no UGI

    Plasmid

    #135350

    Purpose

    Expression of intradomain insertion of rat cytosine deaminase, rAPOBEC3, at position 1058 of nSpyCas9 lacking uracil DNA glycosylase inhibitor.

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    nSpyCas9-rAPOBEC3-ID-1058
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. S1 1029 AIDx with UGI

    Plasmid

    #135354

    Purpose

    Expression of nSpyCas9 encoding truncated human cytosine deaminase hAIDx at amino acid 1029 and uracil DNA glycosylase inhibitor (UGI) at the C terminus

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    hAIDx-nSpyCas9 aa-UGI
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pPICZ-His6-K2P4.1B-mCherry-StreptagII

    Plasmid

    #158744

    Purpose

    Expresses K2P4.1 isoform B with TEV protease cleavable N- and C-terminal affinity tags.

    Depositor

    Arthur Laganowsky

    Article

    Schrecke et al Nat Chem Biol. 2021 Jan;17(1):89-95. doi

    Insert

    K2P4.1-B (KCNK4 Human)
    

    Use

    Tags

    His6 and mCherry with Streptag II

    Expression

    Yeast

    Mutation

    Residues 1-290 and N-linked glycosylation sites r…

    Promoter

    AOX1

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge