Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 23,118 results for: Sis

Clear keywords
Showing: 3151 - 3200 of 23118 results

  3. 3xFLAG-dCas9/p-bacteria

    Plasmid
    #64325
    Purpose
    Expresses 3xFLAG-dCas9 in E. coli in a tetracycline-dependent manner for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Fujita et al BMC Res Notes. 2018 Jun 14;11(1):387. do
    Insert
    3xFLAG-dCas9
    Use
    CRISPR; Tetracycline-inducible
    Tags
    3xFLAG
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Promoter
    pLtetO-1
    Available Since
    Aug. 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    64325-plasmid-map-sequence-id-107969

    Enlarge

  12. pJ151-HDR

    Plasmid
    #113631
    Purpose
    Contains two recombination homology arms and loxP-flanked cassette encoding puromycin resistance gene, Venus fluorescence marker, thymidine kinase suicide gene, for scarless chromosomal engineering.
    Depositor
    Alex Kentsis
    Inserts
    Left homology arm
    Puromycin resistance gene
    Venus fluorescence gene
    Thymidine kinase
    Right homology arm
    Use
    Cre/Lox; Homology recombination selection vector
    Expression
    Bacterial and Mammalian
    Promoter
    EF1a promoter, EF1a promoter (T2A cleaving sequen…
    Available Since
    Sept. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    113631-plasmid-map-sequence-id-222216

    Enlarge

  16. pSBY3_ligD-HygR

    Plasmid
    #104625
    Purpose
    CRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn Cpf1 and crRNA targeting ligD and hygromysin resistance gene.
    Depositor
    Sheng Yang
    Article
    Sun et al Biotechnol J. 2018 Sep;13(9):e1700588. d
    Insert
    FnCpf1
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    FnCpf1 codon-optimized C.glutamicum
    Promoter
    hsp60
    Available Since
    June 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    104625-plasmid-map-sequence-id-201573

    Enlarge

  44. pV1F

    Plasmid
    #205084
    Purpose
    Prokaryotic expressing of AtVIP1 fused with a sequence encoding a T4SS translocation tag in A. tumefaciens.
    Depositor
    Suomeng Dong
    Article
    Wang et al PLoS Pathog. 2023 Apr 21;19(4):e1011346.
    Insert
    AtVIP1
    Expression
    Bacterial
    Promoter
    agorbacterium virB operon promoter
    Available Since
    Aug. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    205084-plasmid-map-sequence-id-405743

    Enlarge

  45. His-DERA

    Plasmid
    #203225
    Purpose
    For protein expression. E. coli 2-deoxy-D-ribose-5-phosphate aldolase. Converts dR5P to G3P and acetaldehyde. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.
    Depositor
    Vern Schramm
    Article
    Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi
    Insert
    deoxyribose aldolase
    Tags
    N-terminal TEV cleavable 6xHis tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    203225-plasmid-map-sequence-id-400074

    Enlarge

  47. pMARO

    Plasmid
    #178086
    Purpose
    Derivative of pMK2010 that contains tetracycline resistance insert between attL1 and attL2 flanked by EcorV. Suitable as control or backbone for library construction for in vivo Gateway reaction.
    Depositor
    Sören Abel
    Article
    Gillman et al Plasmid. 2021 Nov;118:102608. doi: 10.10
    Insert
    TcR (tetA/tetR)
    Expression
    Bacterial
    Mutation
    Tetracycline resistance cassette is flanked by Ec…
    Available Since
    Dec. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    178086-plasmid-map-sequence-id-349998

    Enlarge

  48. pDE43-MCK

    Plasmid
    #49523
    Purpose
    Kanamycin and Chloramphenicol resistant; destination vector engineered to integrate into the mycobacteriophage att-L5 site and designed to be used with the multisite Gateway cloning system
    Depositor
    Dirk Schnappinger
    Article
    Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.
    Type
    Empty backbone
    Expression
    Bacterial
    Available Since
    Jan. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    49523-plasmid-map-sequence-id-73915

    Enlarge

Showing: 3151 - 3200 of 23118 results