We narrowed to 15,323 results for: pET
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Plasmid#169121Purposeexpression of proteinDepositorInserthDDI (DDI2 Human)
ExpressionBacterialAvailable SinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-MBP-TEV-AICD
Plasmid#69927Purposebacterial expression of MBP-TEV-AICDDepositorAvailable SinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SdrG_N2N3-HIS-ybbr
Plasmid#101238PurposeSdrG N2 and N3 domains with C-terminal ybbr tag for covalent immobilizationDepositorInsertSdrG_N2N3
TagsH3V 3C, HIS, and ybbrExpressionBacterialPromoterT7Available SinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET27b-ecDHFR-N23PP/L28F
Plasmid#118584PurposeBacterial expression of E.coli DHFR N23PP and L28F mutantDepositorInsertDHFR
ExpressionBacterialMutationL28 mutated to F28, N23 mutated to P23, a proline…PromoterT7Available SinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-KIX (586-672)
Plasmid#99336PurposeBacterial expression of mouse CBP KIX domainDepositorAvailable SinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TGFα-GSY-SPM
Plasmid#163615PurposeExpresses mature human Transforming Growth Factor alpha fused to SPM in bacterial cells for calcium-induced covalent coupling to Epidermal Growth Factor Receptor.DepositorAvailable SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETDuet RagA(D130N)-RagC
Plasmid#99642PurposeBacterial expression of RagA(D130N)-RagCDepositorAvailable SinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28-6His-SUMO-Nsp14
Plasmid#154956PurposeExpresses Nsp14 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coliDepositorInsert6His-SUMO-Nsp14 (ORF1ab SARS-CoV-2)
Tags6His-Thrombin-SUMOExpressionBacterialPromoterT7 PromoterAvailable SinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET24a HAB1 (172-511)
Plasmid#177844PurposeBacterial Expression of HAB1DepositorInsertProtein phosphatase 2C 16
Tags6xHis-GSTExpressionBacterialAvailable SinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
PET28a-cpLumiLuc-Rapamycin sensor
Plasmid#186292PurposeExpression Rapamycin sensor in e coliDepositorInsertcpLumiLuc-Rapamycin sensor
TagsHisTagExpressionBacterialAvailable SinceAug. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SENP5 (catalytic domain)
Plasmid#16358DepositorInserthuman SENP5 catalytic domain (SENP5 Human)
TagsHisExpressionBacterialMutationAmino Acid 567 to 755 catalytic domain of human S…Available SinceDec. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pETDuet RagA(T21N)-RagC
Plasmid#99644PurposeBacterial expression of RagA(T21N)-RagCDepositorAvailable SinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
PET-B-eSpCas9-NLS-6xHis
Plasmid#207383PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertB-eSpCas9
UseCRISPRTags6xHisExpressionBacterialMutationK848A, K1003A, R1060A, amino acids 1005-1013 repl…PromoterT7Available SinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28-His6-Ubc9-K14R
Plasmid#185759PurposeBacterial expression of His6-Ubc9-K14RDepositorInsertSUMO-conjugating enzyme UBC9 (UBE2I Human)
TagsThrombin-cleavable His6-tagExpressionBacterialMutationK14RPromoterT7Available SinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_LAMA-G97 (for GFP)
Plasmid#130715PurposePlasmid encoding the bacterial expression of LAMA-G97 for GFPDepositorInsertLAMA-G97
TagsHisx10ExpressionBacterialMutationDeleted first Met, Ala and Last Ser from the Enha…Available SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-YPet-WPRE
Plasmid#193342PurposeAAV-mediated expression of YPet under the TRE promoter in mammalian cellsDepositorInsertYPet
UseAAVExpressionMammalianPromoterTREAvailable SinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28b-MBP-TEV-PWWP_DNMT3A
Plasmid#186970PurposeBacterial expression of human DNMT3A PWWP domain (residues 278–427) with His-MBP tag and TEV protease cleavage site.DepositorInsertDNMT3A PWWP domain (DNMT3A Synthetic, Human)
TagsHis-MBPExpressionBacterialMutationPWWP domain (residues 278–427)PromoterT7Available SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET22b-mEB2-eGFP-6xHis
Plasmid#130982PurposeBacterial expression of mouse EB2-GFP-6xHis for protein purification.DepositorAvailable SinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET30a-His6-ABP-LOXCATmut
Plasmid#139972PurposeA control for His6-ABP-LOXCAT where LOX is catalytically dead (mutations H265A and R268A)DepositorInsertA fusion of lactate oxidase from A. viridans and catalase from E. coli
TagsHis6ExpressionBacterialMutationMutation in H265A and R268A in LOX (used original…PromoterT7Available SinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET23-PBD(65-150)-N-Cys
Plasmid#13722DepositorInsertPBD(65-150)-N-Cys (PAK1 Human)
Tags6xHisExpressionBacterialMutationMet-Cys-Gly-PBD(65-150)-Available SinceMarch 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pETDuet RagA-RagC(D181N)
Plasmid#99643PurposeBacterial expression of RagA-RagC(D181N)DepositorAvailable SinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-FLAG-SpCas9-HF1
Plasmid#126770PurposeExpression of increased fidelity SpCas9-HF1 in bacterial cellsDepositorInsertSpCas9-HF1
UseCRISPRTags3xFLAG, 6xHis, MBP, and NLSExpressionBacterialMutationN497A, R661A, Q695A, Q926APromoterT7Available SinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET30a-SARS-CoV2-NP-Nt(47-173)
Plasmid#165091Purposeprotein expression in E. coliDepositorInsertSevere acute respiratory syndrome coronavirus 2 nucleocapsid protein N-terminal(47-173) (N SARS-CoV2)
ExpressionBacterialAvailable SinceFeb. 22, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28-his6-HA3-HALO
Plasmid#188943PurposeT7 expression vector for making his6- and HA3-tagged HALO proteinDepositorInserthis6-HA3-HALO
ExpressionBacterialAvailable SinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET151-D-TOPO-PARP14-SΔK
Plasmid#216658PurposeExpresses multidomain construct of PARP14 with internal SUMO domain (PARP14-SΔK) in bacterial cells.DepositorInsertPARP14-SΔK DNA sequence (PARP14 G784-K1808 with internal SUMO-TEV sequence) (PARP14 Synthetic, Human)
Tags6xHis-V5-TEVExpressionBacterialMutationDeletion starting from Q1390 to D1448 and substit…PromoterT7Available SinceAug. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET22b-mEB3-mCherry-6xHis
Plasmid#130985PurposeBacterial expression of mouse EB3-mCherry-6xHis for protein purification.DepositorAvailable SinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pETM10 with rat FL Annexin A11
Plasmid#164496PurposeFor bacterial expression of rat His-tagged FL Annexin A11DepositorAvailable SinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET22-His-TEV-DOT1L-MM
Plasmid#89611PurposeBacterial expression vector for an N-terminally His-tagged (TEV-cleavable) version of human Dot1L hypermorphic MM mutant residues 1-416DepositorAvailable SinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-StrepII-TagRFP-CohI
Plasmid#58710PurposeDetection construct to label dockerin type I containing fusion proteinsDepositorInsertTagRFP-CohI
TagsStrepIIExpressionBacterialPromoterT7Available SinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET21a(+)-Histag-Cerulean (526)
Plasmid#70716PurposeExpression vector for Cerulean purificationDepositorInsertCerulean
Available SinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
Pet15b-Endosomal Escape Control
Plasmid#186552PurposeNegative Control confirming the need for a CPP peptide in mediating internalizationDepositorInsert6H-ETA-GFP
Tags6xHis and EGFPExpressionBacterialPromoterT7Available SinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pet15b-Internalization and Endosomal Escape
Plasmid#186551PurposeInternalization and Endosomal Escape to effectively route a protein cargo to the cytosol of cellsDepositorInsert10R-8His-ETA-EGFP
Tags8xHis and EGFPExpressionBacterialPromoterT7Available SinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28b-LanCL2 (6xHis-LanCL2)
Plasmid#73407PurposeExpresses His tagged LanCL2DepositorAvailable SinceApril 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETconNK-TEM1(S70A,D179G)
Plasmid#81166PurposepETconNK with TEM1(S70A,D179G) beta-lactamaseDepositorInsertTEM-1 beta-lactamase
Tagsc-mycExpressionBacterial and YeastMutationDeleted the sec transport tag. Plasmid encodes H2…PromoterGAL1 and gal1Available SinceFeb. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET22b 6His SpyCatcher-GRFT
Plasmid#204502PurposeBacterial expression of GRFT with N-terminal SpyCatcher fusion and GS linkerDepositorInsertGRFT
UseSynthetic BiologyTags6xHis and SpyCatcherExpressionBacterialPromoterT7Available SinceSept. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a_DmKHC[1-421]-SNAP-6xHis
Plasmid#196975PurposeBacterial expression plasmid for His tag-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal SNAP tag and C-terminal 6xHis tagDepositorAvailable SinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+) 6xHis-TEV-IFIT3
Plasmid#53559Purposebacterial expression of IFIT3DepositorAvailable SinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AMPKsub-YPet-NES
Plasmid#84635PurposeEncodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertAMPKsub-YPet-NES
Tags6xHis, Nuclear export signal (NES), T7 tag (gene …ExpressionMammalianPromoterCMVAvailable SinceApril 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21a-Med25-VBD-6His
Plasmid#64773PurposeBacterial expression of His-tagged Med25-VBDDepositorAvailable SinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-tTALE-mStrawberry-15b
Plasmid#206868PurposeExpress tTALE-mStrawberryDepositorInsert7-mer tTALE-mStrawberry
ExpressionBacterialPromoterT7 promoterAvailable SinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42-ARF6-V5-His6
Plasmid#67415PurposeExpression of hArf6 with C-term V5 and His6 tag in E. coliDepositorAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET51b-His-TEV-HaloTag7_dead_mt
Plasmid#167267PurposeOverexpression of the dead variant (D106A) of HaloTag7 in E. coliDepositorInsertHaloTag7
TagsHis x10ExpressionBacterialMutationD106APromoterT7Available SinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTS2396-Tier1-PhCMV-4xCOX8_Ypet
Plasmid#169534PurposeTier-1 vector encoding PhCMV-driven yellow fluorescent protein YPet localized to the mitochondria (PhCMV-4xCOX8-YPet-pA).DepositorInsertPCMV-driven miochondria-localized yellow fluorescent protein Ypet
ExpressionMammalianPromoterPhCMVAvailable SinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+) 6xHis-TEV-IFIT2
Plasmid#53558Purposebacterial expression of IFIT2DepositorAvailable SinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-deSpCas9-VP64-6xHis
Plasmid#92117PurposeExpression of dead/inactive increased fidelity eSpCas9 (1.1)-VP64-6xHis in bacterial cellsDepositorInsertdead/inactive eSpCas9-NLS-3xFLAG-VP64
UseCRISPRTags3xFLAG, 6xHis, NLS, and VP64ExpressionBacterialMutationD10A, H840A, K848A, K1003A, R1060APromoterT7Available SinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET24a-VHH-tev-std
Plasmid#109423PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody with a TEV site. VHH-tev-std is flanked by an N-terminal T7 epitope, and a C-terminal cassette composed of HA, BAP and a His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, TEV cleavage site, HA, BAP and His6 epitope
TagsBAP, HA, His6, and T7ExpressionBacterialPromoterT7Available SinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-FKBP-STING(pLxIS)
Plasmid#221333PurposeOverexpression of STING CTT in bacteriaDepositorAvailable SinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-pro-siRNA-V2-TP53
Plasmid#111089PurposepET-pro-siRNA-V2 based plasmid for production of recombinant siRNAs (pro-siRNAs) against human TP53 gene.DepositorInsertTP53 (TP53 Human)
ExpressionBacterialAvailable SinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28-sfGFP-DogTag Loop B
Plasmid#171780PurposeExpresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Asp102 and Asp103DepositorInsertsfGFP-DogTag Loop B
TagsHis6ExpressionBacterialMutationDogTag and linkers inserted between residues Asp1…PromoterT7Available SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
HOXA4 (murine) HA-tag pET
Plasmid#8489DepositorInsertHOXA4 (Hoxa4 Mouse)
TagsHA and T7ExpressionBacterialMutationThr-2 changed to Ala-2 to form better ribosomal b…Available SinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only