Addgene

We narrowed to 4,899 results for: puc

Showing: 3151 - 3200 of 4899 results

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qTAG-N-Puro-sTagRFP

Plasmid

#207690

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-sTagRFP cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-N-Blast-sTagRFP

Plasmid

#207679

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-sTagRFP cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pAM752

Plasmid

#207891

Purpose

Level 0 for A5 module (5' ACCT-3' TCCC)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

dummy 20nt sequence

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAM761

Plasmid

#207900

Purpose

Level 0 for C7 module (5' GGCT-3' TTCG)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

dummy 20nt sequence

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAM759

Plasmid

#207898

Purpose

Level 0 for C5 module (5' AGCC-3' TCAG)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

dummy 20nt sequence

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAM760

Plasmid

#207899

Purpose

Level 0 for C6 module (5' TTCG-3' TCAG)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

dummy 20nt sequence

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAM764

Plasmid

#207903

Purpose

Level 0 for D3 module (5' TCCC-3' CTGC)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

dummy 20nt sequence

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAM767

Plasmid

#207906

Purpose

Level 0 for E1 module (5' CTGC-3' CCAT)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

dummy 20nt sequence

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAM751

Plasmid

#207890

Purpose

Level 0 for A4 module (5' TGAC-3' AACA)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

dummy 20nt sequence

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAM753

Plasmid

#207892

Purpose

Level 0 for B1 module (5' AACA-3' CCAT)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

dummy 20nt sequence

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAM762

Plasmid

#207901

Purpose

Level 0 for C8 module (5' GGCT-3' GCAG)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

dummy 20nt sequence

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAM765

Plasmid

#207904

Purpose

Level 0 for D4 module (5' TGAC-3' CTGC)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

dummy 20nt sequence

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAM766

Plasmid

#207905

Purpose

Level 0 for D5 module (5' TCAG-3' TCCC)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

dummy 20nt sequence

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAM754

Plasmid

#207893

Purpose

Level 0 for B2 module (5' CCAT-3' GGCT)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

dummy 20nt sequence

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAM763

Plasmid

#207902

Purpose

Level 0 for D1 module (5' TCAG-3' TGAC)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

dummy 20nt sequence

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAM768

Plasmid

#207907

Purpose

Level 0 for E2 module (5' CCAT-3' ACTA)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

dummy 20nt sequence

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
NK621

Plasmid

#196405

Purpose

Strong expression of firefly luciferase codon-optimized for S. rosetta

Depositor

Nicole King

Article

Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092

Insert

Sro5actin-firefly-Sro3actin

Use

Luciferase

Tags

Expression

Mutation

N/A (synthetic)

Promoter

Available since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-Cas12i2-T2A-EGFP

Plasmid

#188270

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Chen et al Innovation (Camb). 2022 May 26;3(4):1002

Insert

Cas12i2

Use

CRISPR

Tags

Expression

Mammalian

Mutation

none

Promoter

CAG

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-AaCas12b-T2A-EGFP

Plasmid

#188271

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Chen et al Innovation (Camb). 2022 May 26;3(4):1002

Insert

AaCas12b

Use

CRISPR

Tags

Expression

Mammalian

Mutation

none

Promoter

CAG

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-PlmCasX-T2A-EGFP

Plasmid

#188273

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Chen et al Innovation (Camb). 2022 May 26;3(4):1002

Insert

PlmCasX

Use

CRISPR

Tags

Expression

Mammalian

Mutation

none

Promoter

CAG

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-TcCas12b-T2A-EGFP

Plasmid

#188277

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Teng et al Cell Discov. 2019 Apr 23;5:23. doi: 10.1

Insert

TcCas12b

Use

CRISPR

Tags

Expression

Mammalian

Mutation

none

Promoter

CAG

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDA313

Plasmid

#195214

Purpose

Expression of superfolder GFP (sfGFP) by a hybrid T7 promoter with tetO.

Depositor

Rahul Sarpeshkar

Article

Deng et al Front Bioeng Biotechnol. 2022 Sep 28;10:

Insert

sfGFP with tetO and a hairpin

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcloDF-amp

Plasmid

#184844

Purpose

Backbone that confers Ampicillin resistance, contains cloDF ori

Depositor

Chase Beisel

Article

Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRISPR

Plasmid

#186276

Purpose

Plasmid containing shortened version of upstream Thiomicrospira CRISPR array for integration assays

Depositor

Jennifer Doudna

Article

Wang et al Nat Commun. 2021 May 6;12(1):2571. doi:

Insert

Thiomicrospira shortened CRISPR array

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
RPL13A_PQR_mScarlet_NOL_LoxP_PQR_zeo_PQR_MCS_RPL13A-3'UTR

Plasmid

#165043

Purpose

Repair template for CAS9 complex genome editing

Depositor

Brian Chen

Article

Lo et al Biotechniques. 2017 Apr 1;62(4):165-174.

Insert

RPL13A (RPL13A Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
PHY55-mouse U6-sgLMNA

Plasmid

#164045

Purpose

U6-driven sgRNA targeting LMNA

Depositor

Baohui Chen

Article

Xu et al Nucleic Acids Res. 2020 Oct 26. pii: 594

Insert

LMNA targeting sgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

mouse U6

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
TC562

Plasmid

#164887

Purpose

Punc-4::rtetR-QFAD(650-777+790-816)::P2A::mKate::T2A::tetR-pie1

Depositor

Tian Chi

Article

Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

Insert

QFAD mutated

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJAID6JY

Plasmid

#165049

Purpose

Integrative expression of TRX1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>TRX1-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3

Use

Tags

Expression

Yeast

Mutation

WT

Promoter

Available since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJAID8Z105

Plasmid

#165057

Purpose

Integrative expression of nucleus-targeting yEGFP-AID*-CUP1 and SKP1-fusing rice auxin receptor gene OsTIR1.

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>SV40NSL>T-PGK1-P-ACS2>SKP1>OsTIR1opt>T-URA3

Use

Tags

Expression

Yeast

Mutation

WT

Promoter

Available since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJAID9L105

Plasmid

#165055

Purpose

Integrative expression of nucleus-targeting yEGFP-AID*-CUP1 and rice auxin receptor gene OsTIR1.

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>SV40NSL>T-PGK1-P-ACS2>OsTIR1opt>T-URA3

Use

Tags

Expression

Yeast

Mutation

WT

Promoter

Available since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJAID9L

Plasmid

#165052

Purpose

Integrative expression of yEGFP and rice auxin receptor gene OsTIR1. OsTIR1 is codon-optimised for expression in S. cerevisiae

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3

Use

Tags

Expression

Yeast

Mutation

WT

Promoter

Available since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJAID5Z

Plasmid

#165047

Purpose

Integrative expression of AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3

Use

Tags

Expression

Yeast

Mutation

OsTIR1 is codon-optimised for expression in S. ce…

Promoter

Available since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pHTR235syn

Plasmid

#133464

Purpose

Consensus sequence of Hop (Humulus lupulus) tandem repeats for cytogenetics

Depositor

Hank Bass

Article

Easterling et al PLoS One. 2020 Jun 5;15(6):e0233971. doi

Insert

HuluTR235

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 11, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pHTR400syn

Plasmid

#133465

Purpose

Consensus sequence of Hop (Humulus lupulus) tandem repeats for cytogenetics

Depositor

Hank Bass

Article

Easterling et al PLoS One. 2020 Jun 5;15(6):e0233971. doi

Insert

HuluTR400

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 11, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pHTR625syn

Plasmid

#133466

Purpose

Consensus sequence of Hop (Humulus lupulus) tandem repeats for cytogenetics

Depositor

Hank Bass

Article

Easterling et al PLoS One. 2020 Jun 5;15(6):e0233971. doi

Insert

HuluTR625

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 11, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pHTR060syn

Plasmid

#133462

Purpose

Consensus sequence of Hop (Humulus lupulus) tandem repeats for cytogenetics

Depositor

Hank Bass

Article

Easterling et al PLoS One. 2020 Jun 5;15(6):e0233971. doi

Insert

HuluTR060

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 11, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pHTR120syn

Plasmid

#133463

Purpose

Consensus sequence of Hop (Humulus lupulus) tandem repeats for cytogenetics

Depositor

Hank Bass

Article

Easterling et al PLoS One. 2020 Jun 5;15(6):e0233971. doi

Insert

HuluTR120

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 11, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pDiySpike3

Plasmid

#136950

Purpose

Plasmid for the In Vitro transcription of DiySpike3

Depositor

Rickard Sandberg

Article

Hagemann-Jensen et al Nat Biotechnol. 2020 Jun;38(6):708-714.

Insert

DiySpike3

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

T7

Available since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDiySpike1d1

Plasmid

#136951

Purpose

Plasmid for the In Vitro transcription of DiySpike1d1

Depositor

Rickard Sandberg

Article

Hagemann-Jensen et al Nat Biotechnol. 2020 Jun;38(6):708-714.

Insert

DiySpike1d1

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

T7

Available since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDiySpike1d2

Plasmid

#136952

Purpose

Plasmid for the In Vitro transcription of DiySpike1d2

Depositor

Rickard Sandberg

Article

Hagemann-Jensen et al Nat Biotechnol. 2020 Jun;38(6):708-714.

Insert

DiySpike1d2

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

T7

Available since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDiySpike1d3

Plasmid

#136953

Purpose

Plasmid for the In Vitro transcription of DiySpike1d3

Depositor

Rickard Sandberg

Article

Hagemann-Jensen et al Nat Biotechnol. 2020 Jun;38(6):708-714.

Insert

DiySpike1d3

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

T7

Available since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDiySpike1d4

Plasmid

#136954

Purpose

Plasmid for the In Vitro transcription of DiySpike1d4

Depositor

Rickard Sandberg

Article

Hagemann-Jensen et al Nat Biotechnol. 2020 Jun;38(6):708-714.

Insert

DiySpike1d4

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

T7

Available since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDiySpike2d1

Plasmid

#136955

Purpose

Plasmid for the In Vitro transcription of DiySpike2d1

Depositor

Rickard Sandberg

Article

Hagemann-Jensen et al Nat Biotechnol. 2020 Jun;38(6):708-714.

Insert

DiySpike2d1

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

T7

Available since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDiySpike2d2

Plasmid

#136956

Purpose

Plasmid for the In Vitro transcription of DiySpike2d2

Depositor

Rickard Sandberg

Article

Hagemann-Jensen et al Nat Biotechnol. 2020 Jun;38(6):708-714.

Insert

DiySpike2d2

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

T7

Available since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDiySpike2d3

Plasmid

#136957

Purpose

Plasmid for the In Vitro transcription of DiySpike2d3

Depositor

Rickard Sandberg

Article

Hagemann-Jensen et al Nat Biotechnol. 2020 Jun;38(6):708-714.

Insert

DiySpike2d3

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

T7

Available since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDiySpike2d4

Plasmid

#136958

Purpose

Plasmid for the In Vitro transcription of DiySpike2d4

Depositor

Rickard Sandberg

Article

Hagemann-Jensen et al Nat Biotechnol. 2020 Jun;38(6):708-714.

Insert

DiySpike2d4

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

T7

Available since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMV47

Plasmid

#128866

Purpose

Position 1 module- IX301997 (yeast integrative region IX301997)

Depositor

Gilles Charvin

Article

Goulev et al PLoS One. 2019 Aug 28;14(8):e0220694. do

Insert

Yeast integrative region chrIX301997

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pJBL7007

Plasmid

#136946

Purpose

Malachite green aptamer with stability hairpin, bacterial promoter

Depositor

Julius Lucks

Article

Silverman et al ACS Synth Biol. 2019 Feb 15;8(2):403-414

Insert

malachite green aptamer (MGA)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
S11_EcoRV_CAMKIIA_AscI

Plasmid

#128475

Purpose

CAMKII1 promoter

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

CAMKII1 promoter

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
S10_EcoRV_hcSYN_AscI

Plasmid

#128474

Purpose

hcSYN promoter

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

hcSYN promoter

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 3151 - 3200 of 4899 results

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