166,778 results

1. PB_p300_core-dCas9-EGFP_hygro

   Plasmid

   #226431

   Purpose

   PiggyBac transposon vector containing a dox inducible p300 core-dCas9 fusion protein and EGFP reporter. Hygromycin selection marker

   Depositor

   Alon Goren

   Inserts

   p300 core-dCas9-EGFP fusion protein
   rtTA3-IRES-Hygro
   

   Use

   CRISPR and Synthetic Biology; Piggybac transposon

   Tags

   HA tag, P2A-EGFP, and p300 core

   Expression

   Mammalian

   Promoter

   Dox inducible minimal CMV and UbC Promoter

   Available Since

   Sept. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFUW-TetO-ID3

   Plasmid

   #207215

   Purpose

   doxycycline-inducible expression of Human ID3 in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Henriques-Oliveira et al Immunity. 58, 1-20. October 2025.

   Insert

   pFUW-TetO-ID3
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pL6puro-pSV40(4)-pmRevErbA-mScaI3-NLS-Pest

   Plasmid

   #240118

   Purpose

   A lentiviral (red) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by SV40 promoter.

   Depositor

   Achim Kramer

   Article

   Gabriel et al bioRxiv 2025.06.30.661926

   Insert

   murine Nr1d1 promoter+mScarlet-I3-NLS-Pest-Reporter (Nr1d1 Mouse)
   

   Use

   Lentiviral

   Mutation

   added SV40-Enhancer+Ori

   Available Since

   Aug. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. CMV-NF186WT-HA

   Plasmid

   #239774

   Purpose

   Expresses WT rat NF186 with a C-terminal HA tag in mammalian cells.

   Depositor

   Ivana Nikić-Spiegel

   Article

   Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

   Insert

   Nfasc (Nfasc Rat)
   

   Tags

   HA-tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. sgLATS1/2-1

   Plasmid

   #229431

   Purpose

   knockout of LATS1 and LATS2

   Depositor

   Christopher Vakoc

   Article

   Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

   Insert

   sgLATS1/2-1 (LATS1, LATS2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6;bU6;EFS

   Available Since

   Dec. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. Human RNF130-3X FLAG

   Plasmid

   #225331

   Purpose

   Lentiviral expression of human RNF130 with 3x FLAG tag in mammalian cells

   Depositor

   Huda Zoghbi

   Article

   Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

   Insert

   RNF130 (RNF130 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA-3xFLAG only

   Plasmid

   #222243

   Purpose

   Express 3xFLAG only in mammalian cells as a control

   Depositor

   Yuliang Wu

   Insert

   3 x FLAG
   

   Tags

   No

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   Aug. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. GPR156-DuET

   Plasmid

   #213267

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   GPR156 (GPR156 Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. AA177

   Plasmid

   #215983

   Purpose

   Fragmid fragment: (N' terminus) Start codon only

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   Start
   

   Use

   Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. AA178

    Plasmid

    #216046

    Purpose

    Fragmid fragment: (C' terminus) Stop only

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Stop
    

    Use

    Fragment

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGTag Hygro EGFP cLAP +2

    Plasmid

    #194310

    Purpose

    CRISPR/Cas9-mediated generic protein tagging

    Depositor

    Frank Schnütgen

    Article

    Seibert et al MethodsX. 2022 Nov 18;9:101929. doi: 10.

    Insert

    cLap(EGFP)-P2A-Hygro
    

    Use

    Bacterial cloning vector

    Available Since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-β-catenin_S23A,T40A,T41A,T112A

    Plasmid

    #190824

    Purpose

    For mammalian expression of β-catenin with 4 O-GlcNAc sites mutated (S23A, T40A, T41A and T112A) and GFP-tag on its N-terminus.

    Depositor

    Gerald Hart

    Article

    Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

    Insert

    β-catenin (CTNNB1 Chicken)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    S23A, T40A, T41A, T112A

    Promoter

    CMV

    Available Since

    Jan. 9, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. Ppst93-ANME1BS MCR-NG

    Plasmid

    #192772

    Purpose

    Expresses NG-tagged ANME1BS MCR in Methanococcus maripaludis

    Depositor

    William B. Whitman

    Article

    Shao et al Commun Biol. 2022 Oct 20;5(1):1113. doi:

    Insert

    Methyl coenzyme M reductase
    

    Use

    Synthetic Biology; Heterologous expression in met…

    Tags

    Flag-Strep2

    Available Since

    Dec. 7, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pXPR_BRD003 LIPT1-2

    Plasmid

    #184481

    Purpose

    Lentivirus gRNA targeting human LIPT1 gene

    Depositor

    Todd Golub, Peter Tsvetkov

    Article

    Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

    Insert

    LIPT1 (LIPT1 Human)
    

    Use

    Lentiviral

    Available Since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pXPR_BRD003 LIPT1-1

    Plasmid

    #184480

    Purpose

    Lentivirus gRNA targeting human LIPT1 gene

    Depositor

    Todd Golub, Peter Tsvetkov

    Article

    Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

    Insert

    LIPT1 (LIPT1 Human)
    

    Use

    Lentiviral

    Available Since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pVER-LacI

    Plasmid

    #164830

    Purpose

    LacI protein regulates expression of yellow fluorescent protein. For measuring dose-response curves.

    Depositor

    Drew Tack

    Article

    Tack et al Mol Syst Biol. 2021 Mar;17(3):e10179. do

    Insert

    LacI
    

    Use

    Synthetic Biology

    Promoter

    Ptac

    Available Since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTY1-LacI

    Plasmid

    #164831

    Purpose

    LacI protein regulates the expression of a tetracycline resistance gene and yellow fluorescent protein

    Depositor

    Drew Tack

    Article

    Tack et al Mol Syst Biol. 2021 Mar;17(3):e10179. do

    Insert

    LacI
    

    Use

    Synthetic Biology

    Promoter

    Ptac

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGBW-m4133266

    Plasmid

    #152047

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 nsp7

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp7 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;TapTag

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available Since

    May 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. actc1b-mKate2-RhoAa-DN

    Plasmid

    #109526

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to dominant negative RhoAa

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    RhoAa (rhoaa Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    mKate2

    Mutation

    DN

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-HA

    Plasmid

    #235678

    Purpose

    Plasmid control for pcDNA3.1-HA-RAD52-WT and pcDNA3.1-HA-RAD52-∆C

    Depositor

    Simon Powell

    Article

    Jalan et al Nat Commun. 2024 Sep 5;15(1):7776. doi:

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    April 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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21. PCS2+Cas9-mSA

    Plasmid

    #103882

    Purpose

    Cas9 mSA for in vitro transcription for gene editing in Mouse Embryos

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    CAS9-MSA
    

    Use

    CRISPR

    Tags

    MSA (monomeric streptavidin

    Mutation

    none

    Promoter

    SP6

    Available Since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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22. MBP-Benzonase

    Plasmid

    #218192

    Purpose

    Bacterial expression of a periplasmically expressed MBP-tagged Serratia Marcescens NucA (Benzonase); also known as c001

    Depositor

    Cheryl Arrowsmith

    Insert

    nucA
    

    Tags

    MBP

    Expression

    Bacterial

    Available Since

    April 24, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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23. pEGFP-Q74

    Plasmid

    #40262

    Depositor

    David Rubinsztein

    Article

    Narain et al J Med Genet. 1999 Oct;36(10):739-46.

    Insert

    HTT partial exon 1 Q74 (HTT Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    expanded poly-Q

    Promoter

    CMV

    Available Since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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24. pcDNA3-OVA

    Plasmid

    #64599

    Purpose

    Expression of chicken ovalbumin as model antigen for study of MHC class I and class II presentation

    Depositor

    Sandra Diebold, Martin Zenke

    Article

    Diebold et al Gene Ther. 2001 Mar;8(6):487-93.

    Insert

    Ovalbumin
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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25. c-Fos

    Plasmid

    #187905

    Purpose

    Expresses c-Fos in transfected cells

    Depositor

    Jennifer Mitchell

    Article

    Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

    Insert

    cFos (FOS Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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26. ptfLC3

    Plasmid

    #21074

    Purpose

    Mammalian expression of rat LC3 fused to mRFP and EGFP

    Depositor

    Tamotsu Yoshimori

    Article

    Kimura et al Autophagy. 2007;3(5):452-60.

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Tags

    EGFP and mRFP

    Expression

    Mammalian

    Available Since

    May 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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27. OpenCRISPR-1 sgRNA-12nt stem

    Plasmid

    #221567

    Purpose

    Express OpenCRISPR-1 sgRNA (12nt stem loop) in human cells using a U6 promoter. Plasmid also encodes a CMV-driven GFP reporter.

    Depositor

    Ali Madani

    Article

    Ruffolo et al Nature. 2025 Jul 30. doi: 10.1038/s41586

    Insert

    OpenCRISPR-1 sgRNA 12nt stem loop
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 28, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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28. pCW57.1_blasti

    Plasmid

    #194067

    Purpose

    3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blasti

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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29. CAPTURE2_pLVX-EF1a-dCas9-CBio-IRES-zsGreen1

    Plasmid

    #138418

    Purpose

    CAPTURE2.0 vector containing dCas9-CBio, IRES and zsGreen1

    Depositor

    Jian Xu

    Article

    Liu et al Genome Biol. 2020 Mar 5;21(1):59. doi: 1

    Insert

    dCas9
    

    Use

    Lentiviral

    Tags

    BioTAP-tag

    Promoter

    EF1a

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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30. pmEGFP-C1

    Plasmid

    #36412

    Depositor

    Benjamin Glick

    Type

    Empty backbone

    Tags

    mEGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge