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Showing: 3251 - 3300 of 79968 results

  5. pDONR223_HyPer7RgDAAO

    Plasmid
    #217746
    Purpose
    Donor vector for gateway cloning of HyPer7 DAAO, a fusion protein used to measure the uptake of D-amino acids across the plasma membrane.
    Depositor
    Seth Parker
    Article
    Johal et al Cell Rep. 2024 Dec 24;43(12):115069. doi
    Insert
    HyPer7 D amino acid oxidase
    Tags
    Nuclear export signal
    Expression
    Mammalian
    Mutation
    Fused DAAO to the C-terminus of HyPer7 using a Gl…
    Available Since
    Dec. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    217746-plasmid-map-sequence-id-442633

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  7. pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA

    Plasmid
    #68717
    Purpose
    Cre-dependent bicistronic vector expressing mRuby2 and GCaMP6s from a single open reading frame.
    Depositor
    Tobias Bonhoeffer, Mark Huebener, Tobias Rose
    Article
    Rose et al Science. 2016 Jun 10;352(6291):1319-22.
    Has Service
    AAV1
    Insert
    mRuby2-P2A-GCaMP6s
    Use
    AAV
    Mutation
    Cre-dependent expression from inverted open readi…
    Promoter
    CAG-FLEX
    Available Since
    June 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    68717-plasmid-map-sequence-id-120856

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  11. pFRLuc-CoV2-FLucStop

    Plasmid
    #177617
    Purpose
    Dual luciferase reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. In-frame stop codon inserted after FLuc (negative control).
    Depositor
    Junjie Guo
    Article
    Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11
    Insert
    SARS-CoV-2 FSE
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    In-frame stop codon added between FLuc and FSE
    Available Since
    Dec. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    177617-plasmid-map-sequence-id-350043

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  13. pAAV-hSyn1-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA

    Plasmid
    #68720
    Purpose
    Cre-dependent bicistronic vector expressing mRuby2 and GCaMP6s from a single open reading frame.
    Depositor
    Tobias Bonhoeffer, Mark Huebener, Tobias Rose
    Article
    Rose et al Science. 2016 Jun 10;352(6291):1319-22.
    Has Service
    AAV1
    Insert
    mRuby2-P2A-GCaMP6s
    Use
    AAV
    Mutation
    Cre-dependent expression from inverted open readi…
    Promoter
    hSyn1-FLEX
    Available Since
    June 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    68720-plasmid-map-sequence-id-198989

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  18. pcDNA5-EGFP-NLS-T2A-mCherry-PTS1

    Plasmid
    #87827
    Purpose
    Mammalian expression vector template for co-expression of EGFP-tagged and mCherry-tagged proteins using T2A. NLS and PTS1 can be replaced by proteins of interest.
    Depositor
    Andrea Musacchio
    Article
    Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1
    Inserts
    NLS
    PTS1
    Tags
    EGFP and mCherry
    Expression
    Mammalian
    Promoter
    CMV promoter, tetracycline operator
    Available Since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    87827-plasmid-map-sequence-id-175008

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  20. pODN-H2BC11-mR2

    Plasmid
    #183866
    Purpose
    Repair template for the C-terminal tagging of H2B histones with mRuby2 in human cells using CRISPR/Cas9.
    Depositor
    Alisa Piekny
    Article
    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:
    Insert
    H2BC11 homology arms with linker-mRuby2 (H2BC11 Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mRuby2
    Expression
    Mammalian
    Mutation
    Homology arms contain point mutations to remove t…
    Available Since
    July 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    183866-plasmid-map-sequence-id-368215

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  21. pODN-mR2-MYH10

    Plasmid
    #183869
    Purpose
    Repair template for the N-terminal tagging of myosin heavy chain 10 with mRuby2 in human cells using CRISPR/Cas9.
    Depositor
    Alisa Piekny
    Article
    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:
    Insert
    MYH10 homology arms with mRuby2-linker (MYH10 Human)
    Use
    CRISPR; Donor template
    Tags
    mRuby2-linker
    Expression
    Mammalian
    Mutation
    Homology arms contain point mutations to remove t…
    Available Since
    May 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    183869-plasmid-map-sequence-id-362448

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  23. (194) pcDNA3.1-HA-nEPEA-(EAAAK)4-OGT(4,K852A)

    Plasmid
    #160742
    Purpose
    Expresses an inactive truncated OGT (4.5 TPRs, K852A) that can't bind O-GlcNAc fused to the EPEA nanobody. A different nanobody can be inserted by using Sgfl + Sgsl restriction enzymes.
    Depositor
    Christina Woo
    Article
    Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10
    Insert
    HA-nEPEA-(EAAAK)4-OGT(4,K852A)
    Tags
    HA-Tag
    Expression
    Mammalian
    Promoter
    T7/CMV
    Available Since
    May 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    160742-plasmid-map-sequence-id-332924

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  24. pSW002-Pc-TorT(sp)-mNeonGreen

    Plasmid
    #205021
    Purpose
    Broad host-range bacterial expression vector with constitutive Pc promoter. Provides E. coli TorT signal peptide in frame with mNeonGreen (codon optimized for expression in P. fluorescens); for targeting mNeonGreen to the periplasm
    Depositor
    Rosemarie Wilton
    Article
    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:
    Insert
    TorT-mNeonGreen
    Expression
    Bacterial
    Mutation
    mNeonGreen is codon optimized for expression in P…
    Promoter
    Pc
    Available Since
    Sept. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    205021-plasmid-map-sequence-id-407999

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  30. RCAS-sgATG7

    Plasmid
    #75228
    Purpose
    An adaption on the RCAS/tv-a somatic cell gene transfer system, for use in combination with an existing Cas9 background in the cell/mouse of interest. Depositors: Jane Fraser/Noor Gammoh
    Depositor
    Noor Gammoh
    Article
    Fraser et al EMBO Rep. 2019 Oct 4;20(10):e47734. doi:
    Insert
    Autophagy Related 7 (Atg7 Mouse)
    Use
    Retroviral
    Promoter
    U6
    Available Since
    June 2, 2016
    Availability
    Academic Institutions and Nonprofits only

  36. pDD3248 pCS2-mtagBFP2-p2a-NES-arrb2a-(ENLYFQ/S)x3-SV40NLS-sfCherry3C(1-10)

    Plasmid
    #251770
    Purpose
    Blue fluorescent protein-tagged TEVp substrate (three-peat motif) for fluorescent protein reconstitution assay
    Depositor
    Dave Dingal
    Article
    Lim Suan et al Nat Commun 17, 3257 (2026).
    Insert
    arrestin, beta 2a (arrb2a Zebrafish)
    Tags
    NLS (SV40), sfCherry3C large fragment and mTagBFP…
    Expression
    Mammalian
    Promoter
    CMV IE94
    Available Since
    April 9, 2026
    Availability
    Academic Institutions and Nonprofits only
    251770-plasmid-map-sequence-id-504794

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  40. pLVX-EF1_-EGFR- FCRL3-50aa-Chimera-puro

    Plasmid
    #248150
    Purpose
    Lentiviral vector encoding a chimeric protein composed of the transmembrane region of FCRL3 and its truncated cytoplasmic tail (aa 595-644; first 50 residues), fused to the extracellular domain of EGFR.
    Depositor
    Silvia Monticelli
    Article
    Bianchi et al J Exp Med (2026) 223 (1): e20242474.
    Insert
    EGFR-FCRL3 (FCRL3 Human)
    Use
    Lentiviral
    Promoter
    EF1_
    Available Since
    Dec. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    248150-plasmid-map-sequence-id-495277

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  41. pLVX-EF1_-EGFR-FCRL3-93aa-Chimera-puro

    Plasmid
    #248149
    Purpose
    Lentiviral vector encoding a chimeric protein composed of the transmembrane region of FCRL3 and its truncated cytoplasmic tail (aa 595-687; first 93 residues), fused to the extracellular domain of EGFR.
    Depositor
    Silvia Monticelli
    Article
    Bianchi et al J Exp Med (2026) 223 (1): e20242474.
    Insert
    EGFR-FCRL3 (FCRL3 Human)
    Use
    Lentiviral
    Promoter
    EF1_
    Available Since
    Dec. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    248149-plasmid-map-sequence-id-495275

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  42. RC00214-pcsb-35Sp-ccdB-mNG-OCSp-tdT

    Plasmid
    #209019
    Purpose
    Dual-fluorescence acceptor plasmid for one-step insertion of a 5' leader of interest between the CaMV35S promoter and mNeonGreen via BpiI. A tdTomato expression cassette serves as internal control.
    Depositor
    Stephen Long
    Article
    Haas et al Plant Direct. 2026 Apr 15;10(4):e70163.
    Inserts
    ccdB
    mNeonGreen
    tdTomato
    Expression
    Plant
    Mutation
    codon optimized for N. benthamiana
    Promoter
    CaMV35S and Octopine Synthase
    Available Since
    Oct. 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    209019-plasmid-map-sequence-id-461176

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  45. pODN-H2BC11-TagBFP

    Plasmid
    #183867
    Purpose
    Repair template for the C-terminal tagging of H2B histones with mTagBFP in human cells using CRISPR/Cas9.
    Depositor
    Alisa Piekny
    Article
    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:
    Insert
    H2BC11 homology arms with linker-mTagBFP (H2BC11 Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mTagBFP
    Expression
    Mammalian
    Mutation
    Homology arms contain point mutations to remove t…
    Available Since
    May 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    183867-plasmid-map-sequence-id-362447

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  46. PEA1-Nuclease

    Plasmid
    #176528
    Purpose
    Delivers all prime editing nuclease components in a single plasmid
    Depositor
    Paul Thomas
    Article
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Insert
    CbH-Cas9-RT, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    GC to CA point mutation in SpCas9 to restore nucl…
    Promoter
    CbH for Cas9, hU6 for gRNAs
    Available Since
    Dec. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176528-plasmid-map-sequence-id-350557

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  47. ST*-GFP

    Plasmid
    #163667
    Purpose
    Expresses partial length ST in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 (first 113 amino acids) (ST6GAL1 Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    This chimera contains the first 113 amino acids o…
    Available Since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    163667-plasmid-map-sequence-id-322800

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  50. pcDNA3.1 Flag YFP hNKCC1 I674C (NT475)

    Plasmid
    #50875
    Purpose
    Expresses human NKCC1 I674C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    I674C in hNKCC1 in NT17
    Promoter
    CMV
    Available Since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50875-plasmid-map-sequence-id-76348

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Showing: 3251 - 3300 of 79968 results