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173,280 results

Showing: 2011 - 2040 of 173280 results

  7. pLentiRNACRISPR_005 - hU6-DR_BsmBI-EFS-RfxCas13d-NLS-2A-Puro-WPRE

    Plasmid
    #138147
    Purpose
    Expresses RfxCas13d in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa
    Depositor
    Neville Sanjana
    Article
    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.
    Insert
    CasRx
    Use
    Lentiviral
    Tags
    NLS
    Available Since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    138147-plasmid-map-sequence-id-349848

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  12. NG-ABE9

    Plasmid
    #240625
    Purpose
    Adenine base editor with narrowed editing window, recognizing NG PAM
    Depositor
    Tingting Jiang
    Article
    Valdez et al Nat Commun. 2025 Aug 30;16(1):8115. doi:
    Insert
    TadA8e (N108Q/L145T)-linker-nCas9 (D10A, L1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T1337R)
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    TadA8e (N108Q/L145T), Cas9 (D10A, L1111R, D1135V,…
    Available Since
    July 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    240625-plasmid-map-sequence-id-479197

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  13. lentiSAMv2

    Plasmid
    #75112
    Purpose
    lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2, dCas9-VP64, and blast resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Article
    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 and EF1A
    Available Since
    May 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    75112-plasmid-map-sequence-id-305764

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  16. pGD-p19

    Plasmid
    #196326
    Purpose
    35S promoter-driven expression of the tomato bushy stunt virus p19 RNA silencing suppressor
    Depositor
    Zhenghe Li
    Article
    Ganesan et al J Virol. 2013 Oct;87(19):10598-611. doi:
    Insert
    tomato bushy stunt virus p19
    Expression
    Plant
    Promoter
    duplicated cauliflower mosaic virus 35S promoter
    Available Since
    May 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    196326-plasmid-map-sequence-id-390023

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  19. pAAV-DIO-ATLASsnFlp

    Plasmid
    #232352
    Purpose
    Anterograde transsynaptic tracer protein to express in presynaptic glutamatergic neurons that express Cre, Flp payload. Must use with AAV8-fDIO-Reporter viruses infected in postsynaptic cells.
    Depositor
    Don Arnold
    Article
    Rivera et al Nat Methods. 2025 May;22(5):1101-1111. d
    Has Service
    AAV5 and AAV8
    Insert
    ATLASsnFlp
    Use
    AAV
    Tags
    ALFA-Tag
    Promoter
    hSyn
    Available Since
    May 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    232352-plasmid-map-sequence-id-471581

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  26. pAAVS1-NDi-CRISPRi (Gen1)

    Plasmid
    #73497
    Purpose
    Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus with mCherry marker
    Depositor
    Bruce Conklin
    Article
    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.
    Inserts
    dCas9-KRAB-P2A-mCherry
    rtTA
    Use
    CRISPR
    Tags
    HA, KRAB, and NLS
    Expression
    Mammalian
    Mutation
    D10A, H840A (catalytically deactivated Cas9)
    Promoter
    CAG and TRE3G
    Available Since
    Dec. 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    73497-plasmid-map-sequence-id-247304

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  27. EFSp-GFP-YAP (5SA)

    Plasmid
    #174170
    Purpose
    Lentiviral vector to constitutively express overactive YAP (5 LATS phosporylation sites mutated to Ala) under control of the EFS promoter.
    Depositor
    Rod Bremner
    Article
    Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610
    Insert
    YAP (YAP1 Human)
    Use
    Lentiviral
    Tags
    3x FLAG tag
    Expression
    Mammalian
    Mutation
    Five LATS phosphorylation sites (S61, S109, S127,…
    Promoter
    EFS
    Available Since
    Sept. 28, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    174170-plasmid-map-sequence-id-343725

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  28. pLenti6M CD38

    Plasmid
    #194125
    Purpose
    Expresses CD38 protein in mammalian cells
    Depositor
    Jun Liu
    Insert
    CD38 (CD38 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    194125-plasmid-map-sequence-id-380720

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Showing: 2011 - 2040 of 173280 results