165,688 results

1. Lenti-guide-puro mAtp5c-2

   Plasmid

   #198480

   Purpose

   lentiviral stable expression of mAtp5c gRNA 2

   Depositor

   Suzanne Pfeffer

   Article

   Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

   Insert

   mAtp5c (Atp5c1 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti-guide-puro mCert1-1

   Plasmid

   #198487

   Purpose

   lentiviral stable expression of mCert1 gRNA 1

   Depositor

   Suzanne Pfeffer

   Article

   Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

   Insert

   mCert1 (Col4a3bp Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lenti-guide-puro mSptlc2 - 2

   Plasmid

   #198494

   Purpose

   lentiviral stable expression of mSptlc2 gRNA 2

   Depositor

   Suzanne Pfeffer

   Article

   Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

   Insert

   mSptlc2 (Sptlc2 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lenti-guide-puro mPHB2-1

   Plasmid

   #198483

   Purpose

   lentiviral stable expression of mPHB2 gRNA 1

   Depositor

   Suzanne Pfeffer

   Article

   Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

   Insert

   mPhb2 (Phb2 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lenti-guide-puro mHgs-2

   Plasmid

   #198482

   Purpose

   lentiviral stable expression of mHgs gRNA 2

   Depositor

   Suzanne Pfeffer

   Article

   Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

   Insert

   mHgs (Hgs Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Lenti-guide-puro mCct8 - 1

   Plasmid

   #198503

   Purpose

   lentiviral stable expression of mCct8 gRNA 1

   Depositor

   Suzanne Pfeffer

   Article

   Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

   Insert

   mCct8 (Cct8 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-MS2-HA-HsRoquin2_AE

   Plasmid

   #148614

   Purpose

   Mammalian Expression of HsRoquin2

   Depositor

   Elisa Izaurralde

   Article

   Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

   Insert

   HsRoquin2 (RC3H2 Human)
   

   Expression

   Mammalian

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV sgRNA-Hic1 GFP

   Plasmid

   #106953

   Purpose

   Lentivirus encoding sgRNA targeting murine Hic1. Includes GFP marker.

   Depositor

   Albert Edge

   Article

   Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

   Insert

   anti-Hic1 sgRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET30-T7-lacO-His-sfGFP

   Plasmid

   #180754

   Purpose

   pET30 vector with sfGFP-S-tag inserted between the Bgl II and T7 terminator

   Depositor

   Michael Black

   Article

   Jew et al Front Bioeng Biotechnol. 2022 Jun 23;10:

   Insert

   superfolder green fluorescent protein
   

   Tags

   strep tag

   Expression

   Bacterial

   Mutation

   G140C- Please see depositor comments

   Available Since

   July 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR221-SLC29A2_STOP

    Plasmid

    #161305

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC29A2 (SLC29A2 Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 15, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. AAVS1 intron 1 gRNA as2

    Plasmid

    #132394

    Purpose

    Targets AAVS1 intron 1, gRNA: AGAACCAGAGCCACATTAAC, MLM3636 backbone

    Depositor

    Gerold Schmitt-Ulms

    Article

    Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

    Insert

    gRNA targeting AAVS1 intron 1 (PPP1R12C Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pIGc-Xph20

    Plasmid

    #133024

    Purpose

    Expresses Xph20 in bacteria

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

    Insert

    Xph20
    

    Tags

    His10

    Expression

    Bacterial

    Mutation

    S63K

    Promoter

    T7

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. KA2896_pPB-TREtight-Tkmin-cHA-IRESH2BmKO2

    Plasmid

    #124175

    Purpose

    piggyBac-based Dox-inducible expression (very weak)

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. KA2895_pPB-TREtight-TATAmin-cHA-IRESH2BmKO2

    Plasmid

    #124174

    Purpose

    piggyBac-based Dox-inducible expression (weak)

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSL1765 (pSPIN-R, R*MmeI)

    Plasmid

    #160734

    Purpose

    Single-plasmid V. cholerae CAST. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA with BsaI sites. Reverse (R) order minimizes self-targeting; MmeI site for Tn-seq; pBBR1 backbone.

    Depositor

    Samuel H. Sternberg

    Article

    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

    Insert

    VchCAST proteins, crRNA, and donor DNA
    

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLifeAct-miRFP670nano

    Plasmid

    #127434

    Purpose

    F-Actin labeling with near-infrared fluorescent protein miRFP670nano

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

    Insert

    LifeAct-miRFP670nano
    

    Tags

    miRFP670nano

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. ChromosomesMembranesandCytoskeleton

    Plasmid

    #62450

    Purpose

    MXS_chaining vector with 4 cassettes

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Inserts

    CMV::H2B-TagBFPx3-bGHpA
    CMV::Lyn-Ceruleanx3-bGHpA
    CMV::mCherryx3-linker-betaActin-bGHpA
    CMV::Citrinex3-linker-alphaTubulin-bGHpA
    

    Use

    Synthetic Biology

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYC-C

    Plasmid

    #207114

    Purpose

    HDR donor template with Puromycin resistance and mNeonGreen marker flanked by two multiple cloning sites (MCS) for insertion of left and right homology arms (L- and R-arms) for the targeted locus

    Depositor

    Yifan Cheng

    Article

    Choi et al Proc Natl Acad Sci U S A. 2023 Aug;120(3

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSLQ10844

    Plasmid

    #183956

    Purpose

    CAG-hyperdCas12a-HA-miniVPR-mCherry-T2A-HygroR

    Depositor

    Stanley Qi

    Article

    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

    Insert

    HyperdCas12a
    

    Use

    CRISPR

    Tags

    HA, Hygromycin Resistance, and mCherry

    Expression

    Mammalian

    Mutation

    D832A, D156R, E292R, D235R, D350R

    Promoter

    CAG

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-TORCAR

    Plasmid

    #64927

    Purpose

    FRET-based biosensor for monitoring mTORC1 kinase activity.

    Depositor

    Jin Zhang

    Article

    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

    Insert

    TORCAR (EIF4EBP1 Human, Synthetic)
    

    Tags

    Cerulean and YPet

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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21. pFGC-pcoCas9

    Plasmid

    #52256

    Purpose

    A binary plasmid containing 35SPPDK:pcoCas9:NOS cassette for plant expression of Cas9 and multiple cloning sites for insertion of guide RNA

    Depositor

    Jen Sheen

    Insert

    35SPPDK:pcoCas9:NOS cassette
    

    Use

    CRISPR; Plant expression

    Promoter

    constitutive 35SPPDK promoter

    Available Since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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22. pLJC5-3XHA-EGFP-PEX26

    Plasmid

    #139054

    Purpose

    HA-PEROXO Lentiviral Construct. Expresses a Peroxisomal Tag.

    Depositor

    David Sabatini

    Article

    Ray et al iScience. 2020 May 22;23(5):101109. doi:

    Insert

    3XHA-EGFP-PEX26
    

    Use

    Lentiviral

    Tags

    HA

    Promoter

    UBC

    Available Since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pT7-EGFP-C1-HsEDC3

    Plasmid

    #25032

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

    Insert

    EDC3 (EDC3 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    June 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGL4-AARE-luc2P-Hygro

    Plasmid

    #101787

    Purpose

    Luferase reporter plasmid containing three tandem repeats of the amino acid response element (AARE)

    Depositor

    Seiichi Oyadomari

    Article

    Miyake et al FASEB J. 2016 Feb;30(2):798-812. doi: 10

    Insert

    3xAARE
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Promoter

    minimal TATA-box promoter with low basal activity

    Available Since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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25. pIR-CMV-SCN2A-Variant-4-IRES-mScarlet

    Plasmid

    #209410

    Purpose

    Expression of wild-type neonatal isoform of human SCN2A.

    Depositor

    Al George

    Article

    Thompson et al bioRxiv. 2023 Feb 23:2023.02.23.529757.

    Insert

    SCN2A Variant 4, stabilized (SCN2A Human)
    

    Expression

    Mammalian

    Mutation

    IVS intron inserted after bp 4551 of the SCN2A OR…

    Promoter

    CMV

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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26. LAMP1-SEP-mRuby3

    Plasmid

    #200939

    Purpose

    endolysosome localized pH sensor using sensorsuperecliptic pHluorin (SEP) and mRuby3

    Depositor

    Nir Hacohen

    Article

    Liu et al Science. 2023 Aug 4;381(6657):508-514. d

    Inserts

    LAMP1 (LAMP1 Human)
    SEP-mRuby3
    

    Use

    Lentiviral

    Available Since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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27. ACS1

    Plasmid

    #13746

    Depositor

    John Denu

    Article

    Hallows et al Proc Natl Acad Sci U S A. 2006 Jul 5. 10

    Insert

    Acetyl CoA Synthetase 1 (Acss2 Mouse)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Feb. 23, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCAG-GFP

    Plasmid

    #11150

    Purpose

    Mammalian expression vector for expression of GFP (CAG promoter)

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

    Has Service

    Cloning Grade DNA

    Insert

    EGFP from Aequorea victoria
    

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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29. ER-GCaMP6f

    Plasmid

    #182548

    Purpose

    Expresses GCaMP6f on the cytosolic side of the ER (P-450site)

    Depositor

    Chris Richards

    Article

    Aryal et al Anal Chem. 2022 Jan 21. doi: 10.1021/acs

    Insert

    ER localized P450
    

    Expression

    Bacterial and Mammalian

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV.hSyn.eGFP.WPRE.bGH

    Plasmid

    #105539

    Purpose

    AAV expression of EGFP from hSyn promoter

    Depositor

    James M. Wilson

    Has Service

    AAV1

    Insert

    EGFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    March 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAAV-DIO-ATLASsnFlp

    Plasmid

    #232352

    Purpose

    Anterograde transsynaptic tracer protein to express in presynaptic glutamatergic neurons that express Cre, Flp payload. Must use with AAV8-fDIO-Reporter viruses infected in postsynaptic cells.

    Depositor

    Don Arnold

    Article

    Rivera et al Nat Methods. 2025 May;22(5):1101-1111. d

    Insert

    ATLASsnFlp
    

    Use

    AAV

    Tags

    ALFA-Tag

    Promoter

    hSyn

    Available Since

    May 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. 1GFP/RNase H1 WT

    Plasmid

    #174447

    Purpose

    Express GFP-tagged RNase H1 WT in E. coli.

    Depositor

    Karlene Cimprich

    Article

    Crossley et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

    Insert

    RNase H1 (RNASEH1 Human)
    

    Tags

    His-tag, GFP

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. mCherry-VPS13C-Cter

    Plasmid

    #232866

    Purpose

    Expresses the C-terminal fragments of VPS13C in mammalian cells

    Depositor

    Pietro De Camilli

    Article

    Wang et al Nat Cell Biol. 2025 May;27(5):776-789. d

    Insert

    the C-terminal fragments of VPS13C (VPS13C Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pT3-N90-beta-catenin

    Plasmid

    #31785

    Depositor

    Xin Chen

    Article

    Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

    Insert

    beta-catenin (CTNNB1 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Deletion of aa 1-90- Constitutitvely Active

    Promoter

    EF1alpha

    Available Since

    Oct. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. FUW-Luc-mCherry-puro

    Plasmid

    #183685

    Purpose

    Lentiviral vector expressing luciferase and mCherry.

    Depositor

    Andrew Kung

    Article

    Kimbrel et al Mol Imaging. 2009 May-Jun;8(3):140-7.

    Insert

    Luc-E2A-mCherry-T2A-PuroR
    

    Use

    Lentiviral

    Promoter

    Human Ubiquitin C (UbC) promoter

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pDisplay-GRAB-rDA3m-IRES-EGFP-CAAX

    Plasmid

    #208696

    Purpose

    Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3m and a membrane-localized EGFP in mammalian cells

    Depositor

    Yulong Li

    Article

    Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    GPCR activation based dopamine (DA) sensor GRAB_rDA3m
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. EGFP-CAAX

    Plasmid

    #86056

    Purpose

    A mammalian expression plasmid encoding farnesylation motif of HRAS with N-terminus EGFP.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    Homo sapiens Harvey rat sarcoma (HRAS Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    contains the region from 825-857 of HRAS as a far…

    Promoter

    CMV

    Available Since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pInducer20_EGFP

    Plasmid

    #228878

    Purpose

    Tet-inducible lentiviral vector for expression of optimized GFP

    Depositor

    Ramiro Iglesias-Bartolome

    Article

    Branch et al bioRxiv [Preprint]. 2024 Oct 3:2024.10.0

    Insert

    EGFP
    

    Use

    Lentiviral

    Available Since

    Jan. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. v5 Cas9-eVLP

    Plasmid

    #228468

    Purpose

    Expresses MMLVgag(C507V)–Cas9 for producing v5 Cas9-eVLPs

    Depositor

    David Liu

    Article

    Raguram et al Nat Biotechnol. 2024 Nov 13. doi: 10.103

    Insert

    MMLVgag(C507V)–Cas9
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    MMLVgag(C507V)

    Promoter

    CMV

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pRBC-sfGFP wild type

    Plasmid

    #174075

    Purpose

    Plasmid with p15a origin of replication expressing codon optimized superfolder GFP with C-terminal His6 tag, under T7 promoter

    Depositor

    Ryan Mehl

    Article

    Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

    Insert

    Superfolder GFP
    

    Tags

    His-6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge