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61,345 results

Showing: 3351 - 3400 of 61345 results

  5. TRUPATH Triple Ga12

    Plasmid
    #196061
    Purpose
    Encodes a G alpha subunit (GNA12) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlpha12-RLuc8 (GNA12 Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196061-plasmid-map-sequence-id-385874

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  16. mCherry-Ezrin-N-14

    Plasmid
    #55043
    Purpose
    Localization: Focal Adhesions/Membrane, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Ezrin (EZR Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55043-plasmid-map-sequence-id-271041

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  17. G3BP1-Grx1-roGFP2

    Plasmid
    #236548
    Purpose
    Expressing fluorescent Grx1-roGFP2 fusion protein in mammalian cells with localization in stress granules.
    Depositor
    Pablo Rivera-Fuentes
    Insert
    G3BP1-Grx1-roGFP2 (G3BP1 Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    236548-plasmid-map-sequence-id-470046

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  19. pcDNA-dCas9-FLp300

    Plasmid
    #61356
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of FL human p300 (aa 2-2414) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal full length human p300 (aa 2-2414) (EP300 Synthetic, Human, S. pyogenes)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    CMV
    Available Since
    April 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    61356-plasmid-map-sequence-id-498727

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  20. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR

    Plasmid
    #118154
    Purpose
    Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-P2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    See depositor comments below
    Promoter
    EF1a core and U6
    Available Since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    118154-plasmid-map-sequence-id-239465

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  21. DsRed1 Munc13-4

    Plasmid
    #245001
    Purpose
    Distribution and localization of human wild-type Munc13-4 in cells
    Depositor
    Thomas Martin
    Insert
    UNC13D (UNC13D Human)
    Tags
    DsRed1
    Expression
    Mammalian
    Available Since
    Nov. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    245001-plasmid-map-sequence-id-488316

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  25. TFORF0428

    Plasmid
    #141615
    Purpose
    Lentiviral vector for overexpressing the PBX1 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    PBX1 (PBX1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141615-plasmid-map-sequence-id-400568

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  28. PB53x EF1-Dendra2-RPB1Amr

    Plasmid
    #81228
    Purpose
    expresses Dendra2-Rpb1 fusion in mammalian cells. Possible to insert into the genome using the Piggibac system
    Depositor
    Timothée Lionnet
    Article
    Cho et al Elife. 2016 May 3;5. pii: e13617. doi: 1
    Insert
    Dendra2 - Rpb1 (alpha amanitin resistant) (POLR2A Human)
    Tags
    Dendra2
    Expression
    Mammalian
    Mutation
    N792D alpha amanitin resistance mutation (Bartolo…
    Promoter
    EF1a
    Available Since
    Nov. 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    81228-plasmid-map-sequence-id-394051

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  32. HA-Clover-HIF-1alpha Wildtype

    Plasmid
    #163365
    Purpose
    Express HA- and Clover-tagged HIF-1alpha wildtype plasmid
    Depositor
    Violaine Sée
    Insert
    Hypoxia Inducible Factor 1 alpha (HIF1A Human)
    Tags
    HA- and Clover-tagged
    Expression
    Mammalian
    Mutation
    Wildtype
    Promoter
    CMV Promoter
    Available Since
    Jan. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    163365-plasmid-map-sequence-id-322246

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  33. PC1575

    Plasmid
    #232088
    Purpose
    Mammalian expression plasmid for ubiquibody (uAb) cloning backbone. Includes an Esp3I restriction site directly upstream of GSGSG linker and CHIPΔTPR CDS and a C-terminal IRES-mCherry cassette.
    Depositor
    Pranam Chatterjee
    Article
    Bhat et al Sci Adv. 2025 Jan 24;11(4):eadr8638. doi
    Insert
    uAb with cloning Esp3I restriction site
    Tags
    IRES-mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    232088-plasmid-map-sequence-id-464073

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  43. tdTurboRFP-Mito-7

    Plasmid
    #58062
    Purpose
    Localization: Mitochondria, Excitation: 553, Emission: 576
    Depositor
    Michael Davidson
    Insert
    Mito (COX8A Human)
    Tags
    tdTurboRFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 4, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  45. EBFP2-Fibrillarin-7

    Plasmid
    #55241
    Purpose
    Localization: Nucleoli, Excitation: 383, Emission: 448
    Depositor
    Michael Davidson
    Insert
    Fibrillarin (FBL Human)
    Tags
    EBFP2
    Expression
    Mammalian
    Mutation
    R36Q, S66F
    Promoter
    CMV
    Available Since
    Oct. 10, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 3351 - 3400 of 61345 results