163,584 results

1. pACYCDuet-1:ChrA

   Plasmid

   #225079

   Purpose

   Expresses precursor peptide ChrA in E.coli

   Depositor

   Wilfred van der Donk

   Article

   Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

   Insert

   ChrA
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAW014-2

   Plasmid

   #85612

   Purpose

   Multi-gRNA delivery vector containing ugtP-gRNA.P395T for Bacillus subtilis

   Depositor

   Perry Chou

   Article

   Westbrook et al Appl Environ Microbiol. 2016 Jun 3. pii:

   Insert

   ugtP-gRNA.P395T
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEIAV-SIN6.1 CGFPW

   Plasmid

   #44171

   Depositor

   John Olsen

   Article

   McKay et al Gene Ther. 2006 Apr;13(8):715-24.

   Insert

   enhanced green fluorescent protein
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   chromophore mutations

   Promoter

   CMV

   Available Since

   May 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPICZalphaB-SapL3

   Plasmid

   #78171

   Purpose

   Expression of Saporin L3 in pichia

   Depositor

   Vern Schramm

   Article

   Yuan et al Biochemistry. 2015 Jul 28;54(29):4565-74

   Insert

   Saporin L3
   

   Use

   Tags

   alpha factor

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   July 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV_BiPVe4_Gq_dTomato

   Plasmid

   #213938

   Purpose

   AAV construct expressing HA-hM3Dq-dTomato driven by chandelier cell-targeting enhancer. Alias: pAAV_WDC0004_Gq_dTomato

   Depositor

   Gordon Fishell

   Article

   Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

   Insert

   HA-GqDREADD-dTomato
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pK27Sumo_His-SUMO-PLpro (nsp3) (SARS-CoV-2)

   Plasmid

   #169193

   Purpose

   To express SARS-CoV-2 Papain-like protease in E. coli

   Depositor

   John Diffley

   Article

   Lim et al Biochem J. 2021 Jul 16;478(13):2517-2531

   Insert

   14His-SUMO-nsp3_E746-K1060 (pp1ab_E1564-K1878) (ORF1ab Synthetic, SARS-CoV-2)
   

   Use

   Tags

   14His-SUMO

   Expression

   Bacterial

   Mutation

   Codon optimised for E. coli

   Promoter

   T5

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pACλCI-β-flap (831-1057)

   Plasmid

   #53733

   Purpose

   Expression of λCI fused via three alanines to E. coli RNAP beta subunit, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.

   Depositor

   Ann Hochschild

   Article

   Dove et al Nature. 1997 Apr 10;386(6625):627-30.

   Insert

   λCI-β-flap fusion
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac UV5

   Available Since

   June 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBRα-β flap (831-1057)

   Plasmid

   #53734

   Purpose

   Expression of alpha NTD fused via three alanines to E. coli RNAP subunit beta, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.

   Depositor

   Ann Hochschild

   Article

   Dove et al Nature. 1997 Apr 10;386(6625):627-30.

   Insert

   alpha NTD-Beta flap fusion
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lpp/lacUV5 (tandem promoter)

   Available Since

   June 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDRF1-GW ymNeongreen-T2A-mCherry

   Plasmid

   #125700

   Purpose

   Produces equimolar expression of yeast codon optimized mNeongreen and mCherry

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   ymNeongreen-T2A-mCherry
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET41b+_Npl4

    Plasmid

    #117108

    Purpose

    Production of recombinant Npl4 in E.coli

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    Npl4 (NPLOC4 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. TFORF1335

    Plasmid

    #142786

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    POU3F2 (POU3F2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. GFP-ERK2

    Plasmid

    #37145

    Depositor

    Rony Seger

    Article

    Rubinfeld et al J Biol Chem. 1999 Oct 22;274(43):30349-5

    Insert

    ERK2 (MAPK1 Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa1-6 deleted as a result of cloning strategy

    Promoter

    CMV

    Available Since

    Nov. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pEBTet-SNAP-Cep152

    Plasmid

    #136825

    Purpose

    Mammalian expression of the centrosomal protein Cep152 N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-Cep152 (CEP152 Synthetic, Human)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNCS mTangerine

    Plasmid

    #91763

    Purpose

    Bacterial expression of a fluorescent protein, monomeric Tangerine (mTangerine)

    Depositor

    Roger Tsien, Erik Rodriguez

    Article

    Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

    Insert

    monomeric Tangerine
    

    Use

    Tags

    no tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CSII-prEF1a-mCherry-3xNLS

    Plasmid

    #125262

    Purpose

    Fluorescent reporter for cell size

    Depositor

    Jan Skotheim

    Article

    Berenson et al Mol Biol Cell. 2019 Nov 15;30(24):2985-2

    Insert

    mCherry-3xNLS
    

    Use

    Lentiviral

    Tags

    3xNLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pENTR TERRA

    Plasmid

    #111383

    Purpose

    TERRA with approximately 130 telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequences

    Depositor

    Klaus Holzmann

    Article

    Kreilmeier et al Genes (Basel). 2016 Aug 16;7(8). pii: ge

    Insert

    TERRA
    

    Use

    Gateway entry cloning

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. MSCV-BCR-ABL1-p190-IRES-EGFP

    Plasmid

    #219964

    Purpose

    Express human BCR-ABL1-P190 in mammalian cells

    Depositor

    Mohamed Fareh

    Insert

    BCR-ABL1-P190 (ABL1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pWY82

    Plasmid

    #65721

    Purpose

    Plasmid with 2.5 kb of telomere sequence

    Depositor

    James Birchler

    Article

    Yu et al Proc Natl Acad Sci U S A. 2006 Nov 14;10

    Insert

    Telomere
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Oct. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-TrxRFP1

    Plasmid

    #98996

    Purpose

    mammalian expression of a biosensor to monitor thioredoxin redox dynamics

    Depositor

    Huiwang Ai

    Article

    Fan et al Nat Chem Biol. 2017 Jun 26. doi: 10.1038

    Insert

    human thioredoxin 1, GS-rich linker, fused with a redox-sensitive RFP
    

    Use

    Tags

    His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Gal:mCherry-CRY2-uTEV1Δ(220-242)

    Plasmid

    #135452

    Purpose

    Yeast expression of uTEV1Δ fused to CRY2 and mCherry

    Depositor

    Alice Ting

    Article

    Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

    Insert

    mCherry-CRY2-TEV1Δ(220-242)
    

    Use

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Promoter

    Gal

    Available Since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge