4,203 results

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LentiCRISPRv2-mCherry

Plasmid

#99154

Purpose

Lentiviral vector encoding sgRNA cloning site + hSpCAS9-P2A-mCherry.

Depositor

Agata Smogorzewska

Inserts

Cas9
mCherry

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EFS-NS

Available Since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only

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AAVS1 T2 CRIPR in pX330

Plasmid

#72833

Purpose

CRISPR/Cas to target the AAVS1 locus in human cells

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

Cas9 and gRNA for targeting the AAVS1 locus in human cells

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoter

Available Since

Feb. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pCS2+HyPer7-NES

Plasmid

#136467

Purpose

Mammalian expression of cytoplasm targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

Depositor

Vsevolod Belousov

Article

Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

Insert

HyPer7

Tags

Nuclear export signal from the HIV Rev protein

Expression

Mammalian

Promoter

CMV, SP6

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pAAVS1-P-CAG-GFP

Plasmid

#80491

Purpose

donor vector for AAVS1 targeting (puromycin selection) and constitutive GFP expression

Depositor

Knut Woltjen

Article

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Insert

CAG-GFP

Use

Donor vector (human)

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pSF3-ultraID

Plasmid

#172878

Purpose

expresses ultraID in mammalian cells with the tet-on or tet-off system

Depositor

Julien Béthune

Article

Kubitz et al Commun Biol. 2022 Jul 4;5(1):657. doi: 1

Insert

ultraID

Use

Luciferase and Retroviral; To be used in a tet-on…

Tags

myc

Expression

Mammalian

Mutation

fragment ([aa2-171]) of aquifex aeolicus BirA wit…

Promoter

TRE-cmv

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pMSP1E3D1

Plasmid

#20066

Purpose

Bacterial expression of an extended membrane scaffold protein (MSP1E3D1) for Nanodisc formation

Depositor

Stephen Sligar

Article

Denisov et al J Biol Chem. 2007 Mar 9. 282(10):7066-76

Insert

MSP1E3D1

Tags

7-His

Expression

Bacterial

Mutation

"extended" MSP1D1; contains repeats of …

Available Since

Jan. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pSIV-D3psi/delta env/delta Vif/delta Vpr

Plasmid

#132928

Purpose

Production of SIV-VLPs containing Vpx protein

Depositor

Jeremy Luban

Article

Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

Insert

Gag, pol, and vpx

Use

Lentiviral

Available Since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pXAT2

Plasmid

#80494

Purpose

AAVS1 sgRNA expression vector

Depositor

Knut Woltjen

Article

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Insert

AAVS1 sgRNA-T2

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pCTcon2

Plasmid

#41843

Depositor

Dane Wittrup

Article

Chao et al Nat Protoc. 2006;1(2):755-68.

Insert

CD20

Tags

HA and c-myc

Expression

Yeast

Available Since

Jan. 23, 2013

Availability

Academic Institutions and Nonprofits only

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Flag-TurboID

Plasmid

#124646

Purpose

Expresses Flag-TurboID fusion for proximity labeling

Depositor

FENG-QIAN Li, Ken-Ichi Takemaru

Type

Empty backbone

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEx-ER-TurboID

Plasmid

#160857

Purpose

Cre recombinase-dependent expression of ER TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection

Depositor

Jonathan Long

Article

Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

Has Service

AAV8

Insert

ER TurboID

Use

AAV

Tags

HA and V5

Promoter

CAG

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pSBbi-Bla

Plasmid

#60526

Purpose

SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: blasticidin resistance gene

Depositor

Eric Kowarz

Article

Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

Type

Empty backbone

Use

Transposon

Expression

Mammalian

Promoter

EF1a/RPBSA

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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H2B-GFP

Plasmid

#11680

Depositor

Geoff Wahl

Article

Kanda et al Curr Biol. 1998 Mar 26. 8(7):377-85.

Insert

Human histone H2B (H2BC11 Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 31, 2006

Availability

Academic Institutions and Nonprofits only

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PPRE X3-TK-luc

Plasmid

#1015

Purpose

PPAR reporter. 3xDR1 sites upstream of a luciferase reporter.

Depositor

Bruce Spiegelman

Article

Kim et al Proc Natl Acad Sci U S A 1998 Apr 14;95(

Insert

PPRE

Use

Luciferase

Expression

Mammalian

Available Since

Nov. 3, 2005

Availability

Academic Institutions and Nonprofits only

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pLVX-M-puro

Plasmid

#125839

Purpose

modifying MCS, replacing EcoRI to BamHI region with BamHI and EcoRI

Depositor

Boyi Gan

Article

Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

Type

Empty backbone

Use

Lentiviral

Available Since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-BRD4

Plasmid

#65378

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

BRD4 (BRD4 Human)

Tags

EmGFP

Expression

Mammalian

Mutation

R1232W

Promoter

CMV

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti-puro

Plasmid

#39481

Purpose

Tetracycline inducible 3rd generation lentiviral vector

Depositor

Ie-Ming Shih

Article

Guan et al Cancer Res. 2011 Nov 1;71(21):6718-27. E

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Tags

V5 and 6XHis

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2012

Availability

Academic Institutions and Nonprofits only

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pLenti-DsRed_IRES_EGFP

Plasmid

#92194

Purpose

Lentiviral overexpression vector to make stable bicistronic cell line for control (EGFP) screen

Depositor

Huda Zoghbi

Article

Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

Insert

DsRed-IRES-EGFP

Use

Lentiviral

Expression

Mammalian

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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pHR-UCOE-SFFV-Zim3-dCas9-P2A-Hygro

Plasmid

#188768

Purpose

dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Zim3-dCas9

Use

Lentiviral

Tags

HA-2xNLS and Zim3 KRAB-NLS fusion

Promoter

SFFV

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pRL-SV40P

Plasmid

#27163

Purpose

Renilla SV40 promoter (no enhancer) reporter gene for normalization of luciferase experiments

Depositor

Ron Prywes

Article

Chen et al Mol Cell Biol. 1999 Jul . 19(7):4695-702

Insert

SV40 promoter

Use

Luciferase

Tags

Renilla Luciferase

Expression

Mammalian

Available Since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only

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