19,585 results
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Plasmid#90214PurposeTo convert human skin fibroblasts into induced motor neurons (hiMN) in combination with ISL1, LHX3, FGF2 and two small molecules, forskolin and dorsomorphin.DepositorAvailable SinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only
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pTALdNC-AAVS1_T1
Plasmid#80495PurposeAAVS1 TALEN expression vector (Left)DepositorInsertAAVS1 TALEN (Left)
UseGateway entryPromoternoneAvailable SinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTALdNC-AAVS1_T2
Plasmid#80496PurposeAAVS1 TALEN expression vector (Right)DepositorInsertAAVS1 TALEN (Right)
UseGateway entryPromoternoneAvailable SinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHAGE_puro
Plasmid#118692PurposeEmpty vector to express overexpression cDNADepositorTypeEmpty backboneUseLentiviralPromoterCMVAvailable SinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE7-P2A-hMLH1dn
Plasmid#222997PurposeMammalian expression of SpCas9 PE7 prime editor with P2A-human MLH1dn (codon optimized)DepositorInsertPE7-P2A-hMLH1dn
TagsSV40 bpNLS and c-Myc NLSExpressionMammalianMutationDeletion of residues 754-756 in MLH1 as described…PromoterCMVAvailable SinceAug. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
phH1-gRNA
Plasmid#53186PurposeExpresses the S. pyogenes sgRNA from the H1 promoterDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRExpressionMammalianPromoterhuman H1Available SinceJune 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-hM3D(Gq)-mCherry
Plasmid#50476PurposeGq-coupled hM3D DREADD fused with mCherry under the control of CaMKIIa promoterDepositorHas ServiceAAV1, AAV2, AAV5, AAV8, and AAV9InserthM3D(Gq)-mCherry (CHRM3 Human)
UseAAVTagsmCherryMutationSee supplemental documents for DREADD mutationsPromoterCaMKIIaAvailable SinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDSW1728
Plasmid#120812PurposeE. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of red fluorescent protein (mCherryOpt)DepositorInsertred fluorescent protein (mCherry), codon-optimized for C. difficile
ExpressionBacterialPromoterPtetAvailable SinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato]
Plasmid#62723PurposeAAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. TdTomato is a codon diversified version.DepositorHas ServiceAAV1 and AAV5InsertChrimsonR-tdTomato
UseAAVTagstdTomatoExpressionMammalianMutationChrimson K176R mutantPromoterhuman synapsin promoterAvailable SinceApril 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-sfGFP
Plasmid#85492PurposepET28a expressing codon optimized superfolder GFP with C-terminal His6 tagDepositorInsertsuperfolder GFP
Tags6x HisExpressionBacterialPromoterT7Available SinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFREE
Plasmid#92050PurposeAllows inducible expression of gRNAs and Cas9 for plasmid curing.DepositorInsertsCas9
gRNA array
UseCRISPRExpressionBacterialPromoterpRhamBAD and ptetAvailable SinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
TRUPATH Triple Gai1
Plasmid#196048PurposeEncodes a G alpha subunit (GNAl1) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteinsDepositorUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmU6-gRNA
Plasmid#53187PurposeExpresses the S. pyogenes sgRNA from the mouse U6 promoterDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRExpressionMammalianPromotermouse U6Available SinceJune 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDY1173_ADAR1p150
Plasmid#193192PurposeExpress full length ADAR1p150DepositorInsertADAR1p150 (ADAR Human)
ExpressionMammalianAvailable SinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
ph7SK-gRNA
Plasmid#53189PurposeExpresses the S. pyogenes sgRNA from the 7SK promoterDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRExpressionMammalianPromoterhuman 7SKAvailable SinceJune 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV_BE4max_P2A_GFP
Plasmid#112099PurposeC:G-to-T:A base editingDepositorInsertBE4max_P2A_GFP
UseCRISPRExpressionMammalianPromoterCMVAvailable SinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
lenti MS2-P65-HSF1_Hygro
Plasmid#61426Purposelenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker NOTE: A version of this plasmid with improved titer is available: Addgene plasmid #89308 lentiMPH v2DepositorInsertMS2-P65-HSF1_2A_Hygro (HSF1 Human, Synthetic)
UseCRISPR and LentiviralExpressionMammalianMutationN55K in MS2PromoterEF1AAvailable SinceDec. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGreen2x35SLUC
Plasmid#108228PurposePlant expression as translational fusions to FLUC with RLUC normalizationDepositorTypeEmpty backboneUseLuciferaseTagsFirefly LuciferaseExpressionPlantPromoter2x35SAvailable SinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmiRFP670-N1
Plasmid#79987PurposeMonomeric near-infrared fluorescent protein miRFP670 in mammalian plasmidDepositorHas ServiceCloning Grade DNAInsertmiRFP670
ExpressionMammalianPromoterCMVAvailable SinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CFP
Plasmid#13030PurposeMammalian expression vector for cloning your gene fused to CFPDepositorHas ServiceCloning Grade DNATypeEmpty backboneExpressionMammalianAvailable SinceNov. 3, 2006AvailabilityAcademic Institutions and Nonprofits only