Showing: 121 - 140 of 17850 results
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Plasmid#85747Purposetetracyline-inducible lentiviral vector with no insert for use in mammalian cellsDepositorTypeEmpty backboneUseLentiviralTagsnoneExpressionMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only
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pSH-Csy4-T2A-SpRFN-P2A-GFP-multi-gRNA
Plasmid#85756PurposeAll in one plasmid that expresses Csy4, S.pyogenes FokI-dCas9-NLS, and GFP. Also encodes for multiplexed gRNAs. Backbone derived from pSQT1601 (Addgene #53369).DepositorInsertsCsy4-T2A-SpRFN-P2A-GFP
gRNA
UseCRISPRTagsCsy4, Csy4 recognition sequence, FokI fusion via …ExpressionMammalianMutationD10A, H840APromoterCMV and U6AvailabilityAcademic Institutions and Nonprofits only -
pKIR1.1
Plasmid#85758PurposeCRISPR/Cas9 in Arabidopsis with seed a fluorescent reporterDepositorInserthuman-codon-optimized SpCas9
UseCRISPRTagsFLAGExpressionPlantMutationPromoterAtRPS5AAvailabilityAcademic Institutions and Nonprofits only -
pET30ax
Plasmid#85761PurposeA bacterial expression vector with a His-tag at N and C-terminus.DepositorTypeEmpty backboneUseTagsHis tag (N-term and C-term)ExpressionBacterialMutationPromoterT7 promoterAvailabilityAcademic Institutions and Nonprofits only -
pSLI-2xFKBP-GFP(1463000)
Plasmid#85789PurposeP. falciparum integration plasmid to fuse endogenous genes with tags using SLIDepositorInsertPF3D7146300 targeting region, 2xFKBP-GFP-2TA-Neo-R
UseP. falciparum integrationTags2xFKBP, GFPExpressionMutationPromoternoneAvailabilityAcademic Institutions and Nonprofits only -
pSLI-sandwich(0807600)
Plasmid#85790PurposeP. falciparum integration plasmid to fuse endogenous genes with tags (double 2xFKBP tag) using SLIDepositorInsertPF3D0807600 targeting region, 2xFKBP-GFP-2xFKBP-2TA-Neo-R
UseP. falciparum genomic integrationTags2xFKBP, GFPExpressionMutationPromoternoneAvailabilityAcademic Institutions and Nonprofits only -
pSLI-TGD(1463000)
Plasmid#85791PurposeP. falciparum integration plasmid to disrupt genes using SLIDepositorInsertPF3D7146300 targeting region, GFP-2TA-Neo-R
UseP. falciparum genomic integration (gene disruptio…TagsGFPExpressionMutationPromoternoneAvailabilityAcademic Institutions and Nonprofits only -
p1xNLS-FRB-mCherry-hsp86-BSD
Plasmid#85795PurposeExpression of 1xNLS mislocaliser in P. falciparumDepositorInsert1xNLS Mislocaliser
UseP. falciparum expressionTagsFRB*, mCherryExpressionMutationPromoterPfhsp86AvailabilityAcademic Institutions and Nonprofits only -
pSkipFlox
Plasmid#85797PurposeP. falciparum plasmid for expression of both diCre fragments from single casette using skip peptideDepositorInsertsplit Cre recombinase
UseCre/Lox; P. falciparum expressionTagsFKBP, FRB*ExpressionMutationPromoterPfcrtAvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-Neo-CAG-M2rtTA-H2BGFP (donor AB)
Plasmid#85798PurposedonorAB targeting vector for human AAVS1 locus; knock-in of Dox controlled H2BGFP expression systemDepositorInsertPPP1R12C
UseHuman targetingTagsExpressionMutationhomology arms for knock-in into first intronPromoterAvailabilityAcademic Institutions and Nonprofits only -
pPS
Plasmid#85801PurposeBackbone for pooled cloning of Perturb-seq librariesDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6AvailabilityAcademic Institutions and Nonprofits only -
pKI1.1R
Plasmid#85808PurposeCRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter.DepositorInserthuman-codon-optimized SpCas9
UseCRISPRTagsFLAG and NLSExpressionPlantMutationPromoterAtRPS5AAvailabilityAcademic Institutions and Nonprofits only -
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pCDNA3_V5-APEX2-HMOX1
Plasmid#85824PurposeExpressed N-term tagged APEX2 on HMOX1 in mammalian cellDepositorInsertV5-APEX2-HMOX1 (HMOX1 Synthetic, Human)
UseTagsAPEX2 and V5ExpressionMammalianMutationPromoterCMV-FAvailabilityAcademic Institutions and Nonprofits only -
Human b2 nicotinic subunit crystallization construct
Plasmid#85842PurposepEZT-BM vector with beta2 gene after CMV promoter, published crystallization constructDepositorInsertHuman b2 nAChR subunit crystallization construct (CHRNB2 Human)
UseBaculovirus (bacmam) production and mammalian exp…TagsC-terminal StrepII tagExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
EZ-Tet-pLKO-Puro
Plasmid#85966PurposeTet/Dox inducible shRNA lentivirus with puromycin selectionDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral and RNAiTagsExpressionMutationPromoterH1 / TetOAvailabilityAcademic Institutions and Nonprofits only -
pBA439
Plasmid#85967PurposePerturb-seq vector backboneDepositorInsertssgGFP-NT2
puro-T2A-BFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pMH0001
Plasmid#85969PurposeUCOE-SFFV-dCas9-BFP-KRABDepositorInsertdCas9
UseLentiviralTagsBFP-KRABExpressionMammalianMutationD10A, H840APromoterSFFVAvailabilityAcademic Institutions and Nonprofits only -
pBA407
Plasmid#85970PurposeUPRE-mCh-Ubc-NeoDepositorInsertsmCherry
Neo
UseLentiviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pBA409
Plasmid#85971PurposeEF1a-sfGFP-Ubc-NeoDepositorInsertssfGFP
Neo
UseLentiviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 121 - 140 of 17850 results