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Plasmid#207664PurposeNeuropeptide degradationDepositorInsertNEP (MME Human)
UseAAV and Cre/LoxTagsmRUBY3ExpressionMutationAddition of signaling peptide (POMC) on the N-ter…PromoterhSynapsinAvailable sinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-MRLC1
Plasmid#35680DepositorInsertMRLC1 (MYL9 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet3-LaminB_C1
Plasmid#189776PurposeConstruct for labelling nuclear envelopes in mammaiian cells using mScarlet3-LaminB fusionDepositorInsertmScarlet3-LaminB
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPTP1BD181A-mCherry
Plasmid#40270DepositorInsertPTPN1 (PTPN1 Human)
UseTagsmCherryExpressionMammalianMutationD181APromoterCMVAvailable sinceNov. 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
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phU6-gRNA
Plasmid#53188PurposeExpresses the S. pyogenes sgRNA from the human U6 promoterDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-LAMP-1-mGFP-IRES-Puromycin
Plasmid#134868PurposeExpresses EGFP-tagged lysosomal markerDepositorInsertLysosomal-associated membrane protein 1 (LAMP1 Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYLCRISPR/Cas9Pubi-H
Plasmid#66187Purposeexpression of Cas9 in plants, maize ubiquitin promoter (Pubi), hygro selectionDepositorInsertCas9
UseTagsExpressionPlantMutationplant-codon optimized with higher GC contents (62…Promotermaize ubiquitin promoter (Pubi)Available sinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAVS1_Puro_Tet3G_3xFLAG_Twin_Strep
Plasmid#92099PurposeDoyon lab Tandem-Affinity Purification Following Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus. Transgene expression is controlled by an Auto-Regulated Tet-On 3G System.DepositorTypeEmpty backboneUseCRISPR and TALEN; ZfnTagsExpressionMammalianMutationPromoterTet-On 3G BidirectionalAvailable sinceJune 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
ER-mRFP
Plasmid#62236PurposePlasmid expresses endoplasmic reticulum targeted mRFP in mammalian cells.DepositorInsertER-mRFP
UseTagsmRFP1ExpressionMammalianMutationPromotercmvAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS513 EF1alpha-cre
Plasmid#11918PurposeExpresses Cre under the control of the elongation factor-1 alpha (EF1alpha) promoter; includes upstream Kozak modification to increase expression.DepositorInsertcre (cre bacteriophage P1)
UseCre/LoxTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Cre.zf1
Plasmid#61391PurposemRNA synthesis of Cre recombinase codon modified for zebrafishDepositorInsertCre.zf1
UseZebrafish expressionTagsExpressionMutationPromotersp6Available sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV enAsCas12f-YHAM-sgRNA_v7
Plasmid#208356PurposeExpression of enAsCas12f-YHAM and sgRNA_v7 in mammalian cellsDepositorInsertenAsCas12f-YHAM
UseTagsExpressionMammalianMutationF48Y/S188H/V232A/E316MPromoterCMVAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pF151 pcDNA3.1(+)SOD1WT
Plasmid#26397DepositorInsertSOD1 (SOD1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 23, 2010AvailabilityAcademic Institutions and Nonprofits only -
His6-MBP-uTEV3
Plasmid#135464PurposeBacterial expression of uTEV3 (full-length) proteaseDepositorInsertHis6-MBP-uTEV3
UseTagsMBP, His6ExpressionBacterialMutationS219V mutation (improves stability) , I138T/S153N…PromoterT5Available sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-NuMA
Plasmid#81029PurposeExpresses human NuMA with N-terminal EGFP tag in mammalian cellsDepositorInsertHomo sapiens nuclear mitotic apparatus protein 1 (NUMA1) (NUMA1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
PLIN2-V5-APEX2
Plasmid#170573PurposeA C-terminal APEX2 fusion to the mouse perilipin-2 protein PLIN2DepositorInsertPLIN2 (Plin2 Mouse)
UseLentiviralTagsV5-APEX2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Vimentin-7
Plasmid#56439PurposeLocalization: Intermediate Filaments, Excitation: 488, Emission: 507DepositorInsertVimentin (VIM Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 13, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC57-TPNOX
Plasmid#87853PurposeThis plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.DepositorInsertTPNOX
UseTagsC-terminal FLAG tag with a linkerExpressionBacterialMutationPromoterAvailable sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
ALIX-mNeonGreen
Plasmid#191191PurposeFull-length ALIX with C-terminal mNeonGreen tag.DepositorInsertALIX (PDCD6IP Human, Synthetic)
UseTags6xHIS and mNeonGreenExpressionMammalianMutationPromoterCMVAvailable sinceOct. 27, 2023AvailabilityAcademic Institutions and Nonprofits only