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169,328 results

Showing: 2071 - 2100 of 169328 results

  2. pAAV-CAG-iGluSnFR4f-NGR-WPRE

    Plasmid
    #234452
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4f-NGR
    Use
    AAV
    Tags
    IgK chain and Myc epi tag-NGR GPI anchor
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234452-plasmid-map-sequence-id-465722

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  4. lenti MS2-P65-HSF1_Hygro

    Plasmid
    #61426
    Purpose
    lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker NOTE: A version of this plasmid with improved titer is available: Addgene plasmid #89308 lentiMPH v2
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature
    Insert
    MS2-P65-HSF1_2A_Hygro (HSF1 Synthetic, Human)
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    N55K in MS2
    Promoter
    EF1A
    Available Since
    Dec. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    61426-plasmid-map-sequence-id-140565

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  6. Ezh2[SET]-dCas9

    Plasmid
    #100087
    Purpose
    Catalytic domain [SET] of mouse Ezh2 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Article
    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99
    Insert
    Ezh2[SET] (Ezh2 Mouse)
    Use
    CRISPR
    Tags
    3XFLag-NLS-Ezh2[SET]-dCas9-NLS
    Expression
    Mammalian
    Mutation
    Ezh2[SET] includes aa 482-746
    Promoter
    CMV
    Available Since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    100087-plasmid-map-sequence-id-197556

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  11. pET-29b(+)_NSP4ss_SerM_NucA-His

    Plasmid
    #218190
    Purpose
    Bacterial expression of a periplasmically expressed His-tagged Serratia Marcescens NucA (Benzonase)
    Depositor
    Florence Pojer
    Article
    Rieubon et al protocols.io 2025
    Insert
    nucA
    Tags
    His and NSP4 signal secretion peptide
    Expression
    Bacterial
    Mutation
    codon optimized ecoli residues - 22-266
    Promoter
    T7
    Available Since
    May 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    218190-plasmid-map-sequence-id-467313

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  12. PB-TO-ASCL1-DLX2

    Plasmid
    #182307
    Purpose
    Piggybac Tet-ON plasmid for differentiating hiPSCs into GABAergic neuron via ASCL1 and DLX2 expression
    Depositor
    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward
    Article
    van Voorst et al bioRxiv [Preprint]. 2025 Jul 7:2025.06.3
    Inserts
    Ascl1 (Ascl1 Mouse)
    Dlx2 (Dlx2 Mouse)
    puroR-T2A-mycNLS-mTagBFP2
    Tags
    None and T2A-mycNLS-mTagBFP2
    Expression
    Mammalian
    Promoter
    EF1a and pTRE3G-bidirectional
    Available Since
    April 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    182307-plasmid-map-sequence-id-359517

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  13. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR

    Plasmid
    #118154
    Purpose
    Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-P2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    See depositor comments below
    Promoter
    EF1a core and U6
    Available Since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    118154-plasmid-map-sequence-id-239465

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  19. mito NAD+ biosensor

    Plasmid
    #186791
    Purpose
    NAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag totarget mitochondria
    Depositor
    Michael Cohen
    Article
    Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d
    Insert
    mito NAD+ biosensor
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    July 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    186791-plasmid-map-sequence-id-368060

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  27. pSA128 A2UCOE-HBB_IVS2-Cas9-BFP

    Plasmid
    #249152
    Purpose
    Overexpression of SpCas9-BFP with HBB IVS2 intron in 5' UTR and blasticidin resistance gene. Contains minimal A2UCOE and Cp36 recombination site.
    Depositor
    Michael Bassik
    Article
    Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi
    Insert
    A2UCOE-HBB_IVS2-Cas9-TagBFP (HBB S. pyogenes Cas9, synthetic, Human)
    Use
    CRISPR; Cp36 donor dna
    Expression
    Mammalian
    Mutation
    HBB IVS2 intron
    Promoter
    EF1a
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249152-plasmid-map-sequence-id-496394

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Showing: 2071 - 2100 of 169328 results