167,150 results
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Plasmid#174724PurposeExpresses GFP-endoNA fusion protein for degradation of polysialic acid. The endoNA is a catalytically active endosialidase from bacteriophage PK1A.DepositorInsertGFP-endoNA fusion protein
TagsHistagExpressionBacterialPromoterT5Available SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCR3-FLAG-TRAF6-C70A
Plasmid#43926DepositorInsertTNF Receptor-Associated Factor 6 (Traf6 Mouse)
Tags2x FLAGExpressionMammalianMutationC70AAvailable SinceJan. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
2xMyc-tdMCP-SNAP (tdMCP standard)
Plasmid#226291PurposeLentiviral plasmid expressing a tdMCP-protein standard. Used in LABEL-seq abundance assays.DepositorInserts2xMyc-tdMCP-SNAPtag
EGFP-IRES-BlastR
UseLentiviralTags2xMyc-tdMCPExpressionMammalianAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
tet-pLKO-sgRNA-puro
Plasmid#104321Purpose3rd generation lentiviral plasmid for inducible expression of sgRNA; derived from tet-pLKO-puro; puromycin selection. See manual for detailed protocols.DepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterH1/TOAvailable SinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT7-hMLH1dn for IVT
Plasmid#178114PurposeTemplate for in vitro transcription of human MLH1dnDepositorInserthuman MLH1dn (codon optimized) (MLH1 Human)
UseTemplate for in vitro transcriptionMutationDetailed in manuscriptPromoterT7 (inactivated)Available SinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
Inducible tdTomato
Plasmid#183718PurposetdTomato expression from dox-inducible promoter; plasmid encodes the TetR inducerDepositorInsertstdTomato
rTetR
UseLentiviralPromoterTRE3GV and UbiCAvailable SinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRT029
Plasmid#127969Purposedouble-DHFR-degron controlled expression of dCas9-BFP-KRAB from the CLYBL locusDepositorInsertCLYBL-CAG-DHFR-dCas9-BFP-KRAB-DHFR
UseTALENExpressionMammalianPromoterCAGAvailable SinceAug. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR_pGK_LaG17_synNotch_Gal4VP64
Plasmid#79127PurposeLentiviral vector for constitutive expression of the LaG17 GFP nanobody synNotch Gal4DBDVP64 Receptor (PGK promoter)DepositorInsertLaG17_synNotch_Gal4DBDVP64
UseLentiviralAvailable SinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTDpelB-NTwinStrep
Plasmid#45940Purposeuse to create N-terminal PelB-Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal PelB-Twin-Strep-tag and C-terminal 6x His-tag for periplasmic translocation via PelB signal sequenceDepositorTypeEmpty backboneTags6xHis, PelB, and Twin-StrepExpressionBacterialPromoterTrc (lactose/IPTG inducible)Available SinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCherry.90 alpha
Plasmid#108222PurposeMammalian expression vector for mCherry fusion to human Hsp90 alphaDepositorAvailable SinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pFH2.105_PB-EPN24-MCP-T2A-eGFP-PGK-Blast
Plasmid#205550PurposeExpress EPN24-MCP-T2A-eGFP and blasticidin resistance markerDepositorInsertEPN24-MCP
UsePiggybacExpressionMammalianMutationWTPromoterEF1Available SinceSept. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
p15aC-GFP
Plasmid#107862PurposeControl plasmid expressing GFPDepositorInsertGFP
TagsmycExpressionBacterialPromoterproDAvailable SinceApril 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
AzoRS-4
Plasmid#182884PurposeA pEvol-based plasmid containing one inducible (by arabinose) copy of the AzoRS-4 aaRS. The 2nd constitutive aaRS copy typically found in pEvol plasmids was deleted.DepositorInsertAzoRS-4
ExpressionBacterialAvailable SinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAHYB-GFP
Plasmid#85074PurposeExpression of GFP in Pichia pastorisDepositorInsertGFP
ExpressionYeastPromoterAOX1Available SinceMarch 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-SFFV-Zim3-dCas9-P2A-BFP
Plasmid#188767PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9
UseLentiviralTagsHA-2xNLS and Zim3 KRAB-NLS fusionPromoterSFFVAvailable SinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-Tp-eyfp
Plasmid#65035PurposeTpR mini-Tn7 vector, mobilizable, for YFP tagging bacteriaDepositorInserteyfp
ExpressionBacterialAvailable SinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pmr101A-cPr-mCherry (KmR)
Plasmid#177207PurposePlasmid with p15A origin for constitutive mCherry expression in E. coliDepositorInsertmCherry
ExpressionBacterialPromoterSynthetic constitutive promoterAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ad-EphA3 WT
Plasmid#102725PurposeExpression of WT EphA3DepositorInsertEphA3 (Epha3 Mouse)
UseAdenoviralAvailable SinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
HNF1B_pLX307
Plasmid#98342PurposeLentiviral expression of HNF1BDepositorAvailable SinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
Rtn3LΔ6LIR-mNeonGreen
Plasmid#169036PurposeExpress human Rtn3L with 6x LC3-interacting regions mutated in mammalian cellsDepositorInsertRtn3L (RTN3 Human)
TagsmNeonGreenExpressionMammalianMutationChanged following LC3-interacting regions of Rtn3…PromoterCMVAvailable SinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-pro-sumo1
Plasmid#25101DepositorAvailable SinceJune 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-PFKM
Plasmid#23728DepositorInsertPFKM (PFKM Human)
UseGateway donor vectorAvailable SinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GCaMP6s
Plasmid#171156PurposeEncodes GCaMP6sDepositorInsertGCaMP6s
ExpressionMammalianAvailable SinceAug. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-IRES eGFP
Plasmid#128059PurposeLentiviral expression of an insert from EF1 promoter and co-expression of EGFP. See Depositor Comments for additional information regarding plasmid sequence.DepositorTypeEmpty backboneUseLentiviralExpressionMammalianAvailable SinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-hsRPA32 (MSW#497)
Plasmid#208068PurposeExpression of GFP-tagged hsRPA2 in human cellsDepositorAvailable SinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRha-ABE7.10-SpCas9-NG
Plasmid#170663PurposeExpresses the base editor ABE7.10-SpCas9-NG in bacterial cellsDepositorInsertABE7.10-SpCas9-NG
Tags8xHIS and BP-NLSExpressionBacterialPromoterpRhaBADAvailable SinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-hsRPA14 (MSW#503)
Plasmid#208069PurposeExpression of GFP-tagged hsRPA3 in human cellsDepositorAvailable SinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-15b-hAR-663-919
Plasmid#89083Purposebacterial expression of human androgen receptor ligand bindng domain amino acid residues 663-919 with histidine tagDepositorInserthAR ligand binding domain (AR Human)
TagsHisExpressionBacterialMutationaa 663-919PromoterT7Available SinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_DNASE1_Exo-endo-phos
Plasmid#110004PurposeProtein expression and purification of DNASE1_Exo-endo-phosDepositorInsertDNASE1_Exo-endo-phos (DNASE1 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only