159,117 results

1. pEGFP-GFP11-Clathrin light chain

   Plasmid

   #70217

   Purpose

   Expresses GFP11-clathrin light chain in mammalian cells

   Depositor

   Bo Huang

   Article

   Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

   Insert

   GFP11-Clathrin light chain (CLTA Human)
   

   Use

   Tags

   GFP11

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMD2.G-K47Q/R354Q

   Plasmid

   #207323

   Purpose

   VSV-G mutant with abolished receptor binding, but maintained fusogenic capacity

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   VSV-G (VSIVgp4 Vesicular Stomatitis Virus)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K47Q/R354Q

   Promoter

   CMV promoter

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-6xHis-SUMO-MarathonRT

   Plasmid

   #109029

   Purpose

   Group II intron maturase from Eubacterium rectale (wild-type sequence). This is a highly processive reverse transcriptase that is called “MarathonRT”

   Depositor

   Anna Pyle

   Article

   Zhao et al RNA. 2018 Feb;24(2):183-195. doi: 10.126

   Insert

   ERE_01490
   

   Use

   Tags

   6xHis-SUMO tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP-C1-PLCdelta-PH

   Plasmid

   #21179

   Purpose

   encodes a PI(4,5)P2 lipid selective PH domain that can be used as a fluorescent translocation biosensor to monitor changes or local differences in the concentration of plasma membrane PI(4,5)P2 lipids

   Depositor

   Tobias Meyer

   Article

   Stauffer et al Curr Biol. 1998 Mar 12. 8(6):343-6.

   Insert

   PLCdelta (PLCD1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   PH domain of human PLC delta, amino acids Met1–Il…

   Promoter

   Available since

   July 10, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pC1-HyPer-Red

   Plasmid

   #48249

   Purpose

   Genetically encoded hydrogen peroxide indicator with red fluorescence.

   Depositor

   Vsevolod Belousov

   Article

   Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

   Insert

   HYPER-RED
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV delta R8.2

   Plasmid

   #12263

   Purpose

   First-generation packaging plasmid. This plasmid contains HIV accessory genes. Please reach out to your biosafety officer for guidance.

   Depositor

   Didier Trono

   Insert

   HIV-1 GAG/POL, Tat and Rev
   

   Use

   Lentiviral; Packaging

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDZ2087

   Plasmid

   #92414

   Purpose

   Expresses His-tagged mutant (L56V/S135G/S219V) TEV protease in E. coli

   Depositor

   David Waugh

   Article

   Raran-Kurussi et al Methods Mol Biol. 2017;1586:221-230. doi

   Insert

   TEV protease
   

   Use

   Tags

   MBP (self cleaving)

   Expression

   Bacterial

   Mutation

   L56V/S135G/S219V

   Promoter

   tac

   Available since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. Flag-HA-USP11

   Plasmid

   #22566

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   USP11 (USP11 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   Nov. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. peYFP-C1-mTOR

   Plasmid

   #73384

   Purpose

   Expresses YFP tagged mTOR

   Depositor

   Taekjip Ha, Jie Chen

   Article

   Jain et al Proc Natl Acad Sci U S A. 2014 Dec 16;11

   Insert

   mTOR (MTOR Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET29a-YS14

    Plasmid

    #66890

    Purpose

    Polycistronic coexpression of Xenopus laevis histones (H2A, H2B, H3 and H4)

    Depositor

    Jung-Hyun Min

    Article

    Shim et al Anal Biochem. 2012 Aug 15;427(2):190-2.

    Inserts

    His6-Thrombin-Xenopus laevis histones H2A
    Xenopus laevis histones H2B
    Xenopus laevis histones H3
    Xenopus laevis histones H4-Thrombin
    

    Use

    Tags

    H2A, H2B, H3, H4, His6 tag, and Thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-28b-Cas9-His

    Plasmid

    #47327

    Purpose

    For in vitro expression and purification of Cas9 protein

    Depositor

    Alex Schier

    Article

    Gagnon et al PLoS One. 2014 May 29;9(5):e98186. doi:

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. Mito-PyronicSF/pCMV-myc-mito

    Plasmid

    #124813

    Purpose

    Highly resposive GFP-based pyruvate nanosensor to explore mitochondrial metabolism.

    Depositor

    Luis Felipe Barros

    Article

    Arce-Molina et al eLife. 2020 Mar 6;9. pii: 53917. doi: 10

    Insert

    PyronicSF
    

    Use

    Tags

    Mitochondrial Sequence Signal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. plenti.CamKII.(cyto).iATPSnFR2.A95A.A119L.miRFP670nano3

    Plasmid

    #209725

    Purpose

    Expresses cytosolic low affinity ratiometric ATP sensor

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    (cyto).iATPSnFR2.A95A.A119L.miRFP670nano3
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CamKII

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET His6 LIC cloning vector (2Bc-T)

    Plasmid

    #37236

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3 APEX2-NES

    Plasmid

    #49386

    Purpose

    Expresses APEX2 in the cytosol of mammalian cells

    Depositor

    Alice Ting

    Article

    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

    Insert

    Flag - APEX2 - NES
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    K14D, W41F, E112K, A134P on soybean APX; improved…

    Promoter

    CMV

    Available since

    Nov. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV-beta-catenin deltaN90

    Plasmid

    #36985

    Depositor

    Bob Weinberg

    Article

    Guo et al Cell. 2012 Mar 2;148(5):1015-28.

    Insert

    beta-catenin deltaN90 (CTNNB1 Human)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 1-90 (Constitutively Acti…

    Promoter

    EF1a

    Available since

    Sept. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. CMV mRuby3-Synapsin1a

    Plasmid

    #187896

    Purpose

    For labeling pre-synaptic terminals

    Depositor

    Timothy Ryan

    Insert

    Synapsin1a
    

    Use

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHSP02

    Plasmid

    #117259

    Purpose

    Genome editing for Lactobacillus plantarum WCSF1

    Depositor

    Sheng Yang

    Article

    Huang et al Biotechnol J. 2019 Jul;14(7):e1800690. d

    Insert

    Cas9, promotor P11-guide-RNA, homologous arms of Lp_0537
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-F-RAM-d2tTA-TRE-mKate2

    Plasmid

    #140274

    Purpose

    F-RAM, the Fos-dependent reporter

    Depositor

    Yingxi Lin

    Article

    Sun et al Cell. 2020 Apr 16;181(2):410-423.e17. do

    Insert

    mKate2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    TRE

    Available since

    July 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCyCBE

    Plasmid

    #226505

    Purpose

    A cytidine deaminase-mediated base-editing plasmid in cyanobacteria

    Depositor

    Weizhong Chen

    Article

    Li et al Commun Biol. 2024 Aug 14;7(1):994. doi:

    Inserts

    APOBEC1-Cas9D10A
    sgRNA
    sacB gene
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge