Addgene

We narrowed to 13,524 results for: TIM

Showing: 2921 - 2960 of 13524 results

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pUB_SM-RLuc100---MS2

Plasmid

#210573

Purpose

Expresses SM(FLAG)-optimal Renilla with 3'UTR MS2 hairpins

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Spaghetti monster (FLAG)-optimal Renilla luciferase

Use

Lentiviral and Luciferase

Tags

Spaghetti monster (FLAG)

Expression

Mammalian

Mutation

Promoter

UBC human

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_SM-FLuc0-malat1

Plasmid

#210580

Purpose

Expresses SM(FLAG)-nonoptimal Firefly - 3'UTR malat1 triple helix

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Spaghetti monster (FLAG)-nonoptimal FLuc - 3'UTR malat1 triple helix

Use

Luciferase

Tags

Spaghetti monster (FLAG)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_SM-FLuc100-malat1

Plasmid

#210581

Purpose

Expresses SM(FLAG)-optimal Firefly luciferase - 3'UTR malat1 triple helix

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Spaghetti monster (FLAG)-optimal FLuc - 3'UTR malat1 triple helix

Use

Luciferase

Tags

Spaghetti monster (FLAG)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pUCmini-UGCG-EGFP

Plasmid

#225878

Purpose

Plasmid donor template for CRISPR/Cas9 mediated UGCG knockout by EGFP insertion

Depositor

Yohei Sato

Article

Sato et al Biochem Biophys Res Commun. 2024 Sep 18;

Insert

EGFP

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pRSETA-qmTQ2-ATP

Plasmid

#225247

Purpose

Bacterial expression of N-terminal His-tagged qmTQ2-ATP, a cyan fluorescent FLIM biosensor for ATP imaging

Depositor

Yasushi Okada

Article

Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

Insert

qmTQ2-ATP

Use

Tags

6XHis, T7 tag, XpressTM tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pRSETA-qmTQ2-glucose

Plasmid

#225252

Purpose

Bacterial expression of N-terminal His-tagged qmTQ2-glucose, a cyan fluorescent FLIM biosensor for detecting glucose

Depositor

Yasushi Okada

Article

Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

Insert

qmTQ2-glucose

Use

Tags

6XHis, T7 tag, XpressTM tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pRSETA-qmTQ2-cAMP

Plasmid

#225249

Purpose

Bacterial expression of N-terminal His-tagged qmTQ2-cAMP, a cyan fluorescent FLIM biosensor for detecting cAMP

Depositor

Yasushi Okada

Article

Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

Insert

qmTQ2-cAMP

Use

Tags

6XHis, T7 tag, XpressTM tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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A99-ELP

Plasmid

#170760

Purpose

A99 laminin α1 peptide fused to I30-ELP (VPGIG)30

Depositor

Andrew MacKay

Article

Truong et al Biomacromolecules. 2024 Jul 8;25(7):4001

Insert

A99-ELP

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pSpyA-GFP

Plasmid

#220189

Purpose

sgRNA expression vector - pEM7 promoter with GFP in sgRNA site

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

pEM7 promoter with GFP in sgRNA site

Use

CRISPR

Tags

Expression

Bacterial

Mutation

monomeric superfolder green fluorescent protein

Promoter

pEM7

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pSpyB-GFP

Plasmid

#220190

Purpose

sgRNA expression vector - pBG35 promoter with GFP in sgRNA site

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

pBG35 promoter with GFP in sgRNA site

Use

CRISPR

Tags

Expression

Bacterial

Mutation

monomeric superfolder green fluorescent protein

Promoter

pBG35

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pSpyD-GFP

Plasmid

#220192

Purpose

sgRNA expression vector - pBG28 promoter with GFP in sgRNA site

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

pBG28 promoter with GFP in sgRNA site

Use

CRISPR

Tags

Expression

Bacterial

Mutation

monomeric superfolder green fluorescent protein

Promoter

pBG28

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pMRS-GFP

Plasmid

#220197

Purpose

mini-Tn7 GFP expression vector

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

monomeric superfolder green fluorescent protein

Use

CRISPR

Tags

Expression

Bacterial

Mutation

monomeric superfolder green fluorescent protein

Promoter

promoterless

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCMJ074

Plasmid

#215654

Purpose

pET28b derived T7 promoter with a 5’-UTR transcriptional fusion to clac143

Depositor

Brian Pfleger

Article

Jones et al Appl Microbiol Biotechnol. 2021 Feb;105(

Insert

Yellow fluorescent protein

Use

Synthetic Biology

Tags

10x histidine tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-C1-ORP5deltaTMD-FKBP

Plasmid

#220073

Purpose

expresses ORP5 with the transmembrane domain deleted. The mCherry fluorophore and FKBP are attached to ORP5.

Depositor

Gerry Hammond

Article

Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

Insert

OSBPL5deltaTMD (OSBPL5 Human)

Use

Tags

Expression

Mammalian

Mutation

deltaTMD

Promoter

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C2-DR

Plasmid

#216266

Purpose

Dox-responsive module fused with EGFP

Depositor

Susumu Tomita

Article

Park et al Cell Rep. 2023 Aug 29;42(8):112826. doi:

Insert

Codon-optimized TetR - Lipid binding motif (KRf - KLK) - Codon-optimized TetR

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

May 8, 2024

Availability

Academic Institutions and Nonprofits only

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pJH4693

Plasmid

#200784

Purpose

Ptwk-40s EGFP unc-54 3' UTR C.elegans AVA and other neurons expression of EGFP

Depositor

Mei Zhen

Article

Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

Insert

no

Use

Tags

GFP

Expression

Worm

Mutation

Promoter

Ptwk-40s

Available since

May 7, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pJH4506

Plasmid

#200255

Purpose

Pnpr-4 TeTx mCherry unc-54 3' UTR C.elegans AVA and other neurons expression of TeTx RFP

Depositor

Mei Zhen

Article

Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

Insert

TeTx

Use

Tags

mCherry

Expression

Worm

Mutation

Promoter

Pnpr-4

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pJH1371

Plasmid

#199631

Purpose

Prgef-1 GPI YFP unc-54 3' UTR C.elegans Pan-neural expression of GPI-YFP

Depositor

Mei Zhen

Article

Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

Insert

GPI

Use

Tags

YFP

Expression

Worm

Mutation

Promoter

Pregf-1

Available since

May 7, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pJH2673

Plasmid

#199691

Purpose

Pnmr-1 FLPe unc-54 3' UTR C.elegans AVA/E/D premotor IN and others expression of FLPe

Depositor

Mei Zhen

Article

Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

Insert

flpE (flpE )

Use

Tags

no

Expression

Worm

Mutation

Promoter

Pnmr-1 long

Available since

May 7, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pYSD063

Plasmid

#212916

Purpose

Encodes mRuby2 fluorescent protein as a Type 3b' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

Depositor

Paul Young

Article

O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

Insert

mRuby2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pBES1-LacOx50-mCh-bla

Plasmid

#210260

Purpose

Integrates 50xLacO repeats and an mCherry gene upstream of the BES1 promoter with a hygromycin drug selection marker downstream of the promoter.

Depositor

Calvin Tiengwe

Article

Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

Inserts

50xLacO repeats derived from: PMID: 12864855.
mCherry

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pCA11-pMa-sgTS3-17xMS2V5-sgTS3

Plasmid

#199442

Purpose

CRISPR donor plasmid to insert 17XMS2V5 into the ribosomal DNA region in dependent of homology arms

Depositor

Baohui Chen

Article

Fu et al J Cell Biol. 2023 Jan 2;222(1):e20220211

Insert

HITI donor template

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-ASAP3ER

Plasmid

#195358

Purpose

ASAP3ER under control of human synapsin promoter

Depositor

Brenda Bloodgood

Article

Campbell et al Cell Rep. 2023 Jan 31;42(1):111943. doi:

Insert

ASAP3ER

Use

AAV

Tags

GFP

Expression

Mammalian

Mutation

Promoter

human synapsin

Available since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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pMET7-GAG-eDHFR

Plasmid

#194635

Purpose

Mammalian expression of the eDHFR control bait in Virotrap

Depositor

Sven Eyckerman

Article

Eyckerman et al Nat Commun. 2016 Apr 28;7:11416. doi: 10

Insert

p55 GAG

Use

Tags

ecDHFR

Expression

Mammalian

Mutation

Promoter

SRalpha

Available since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pUPD2_SulR(CDS)

Plasmid

#186423

Purpose

provides sulfadiazine resistance (sulI) as a level 0 GoldenBraid part (CDS; B3-B4-B5)

Depositor

Lukas Fischer

Article

Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007

Insert

SulR

Use

Synthetic Biology; Cds (part b3-b4-b5), goldenbra…

Tags

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pFR-mScarletI-antiFRET-P2A-EGFP

Plasmid

#191458

Purpose

Compare the intensity of EGFP to mScarlet-I fluorescent protein

Depositor

Dorus Gadella

Article

van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

Insert

EGFP

Use

Tags

His-mScarletI-antiFRETlinker-P2A

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pFR-mScarletI-antiFRET-P2A-mTurquoise2_T203Y

Plasmid

#191459

Purpose

Compare the intensity of mTurquoise2_T203Y to mScarlet-I fluorescent protein

Depositor

Dorus Gadella

Article

van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

Insert

mTurquoise2_T203Y

Use

Tags

His-mScarletI-antiFRETlinker-P2A

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pFR-mTq2-antiFRET-P2A-mCherry

Plasmid

#191466

Purpose

Compare the intensity of mCherry to mTurquoise2 fluorescent protein

Depositor

Dorus Gadella

Article

van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

Insert

mCherry

Use

Tags

His-mTurquoise2-antiFRETlinker-P2A

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_Cep41-STf

Plasmid

#182010

Purpose

CMV overexpression of SNAP-tag fast in mammalian cell lines associated to microtubules

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

Cep41-STf

Use

Tags

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_[Cox8a]x2_STf

Plasmid

#182003

Purpose

CMV overexpression of SNAP-tag fast in the mitochondrial matrix of mammalian cell lines

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

[Cox8a]x2_STf

Use

Tags

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_CalR_STf_KDEL

Plasmid

#182004

Purpose

CMV overexpression of SNAP-tag fast in the endoplasmic reticulum of mammalian cell lines

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

CalR_STf_KDEL

Use

Tags

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_b4g-STf

Plasmid

#182005

Purpose

CMV overexpression of SNAP-tag fast in the golgi apparatus of mammalian cell lines

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

b4g-STf

Use

Tags

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pRB121

Plasmid

#181945

Purpose

aTc-inducible expression of dimerized mNeonGreen (mNG-mNG)

Depositor

Jeffrey Tabor

Article

Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

Insert

mNeonGreen-mNeonGreen; tetR (AX04_RS23485 Synthetic)

Use

Tags

Expression

Bacterial

Mutation

See Depositor Comments Below

Promoter

mNG-mNG-PLtetO-1; tetR-J23106

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pKeratin-Electra2

Plasmid

#184928

Purpose

Keratin labeling with blue fluorescent protein Electra2

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

Keratin-Electra2

Use

Tags

Electra2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pElectra1-LaminA

Plasmid

#179482

Purpose

Nuclear envelope labeling with blue fluorescent protein Electra1

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

Electra1-LaminA

Use

Tags

Electra1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Crimson/P2A-Control (pSLIK3)

Plasmid

#113858

Purpose

An isogenic synaptase-free control vector to evaluate recombination using endogenous functions for N-terminal Crimson/P2A fusion plasmids.

Depositor

Richard S Myers

Article

Valledor et al PLoS One. 2018 Aug 15;13(8):e0200955. do

Insert

E2-Crimson

Use

Lentiviral

Tags

P2A/Crimson

Expression

Mutation

Promoter

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
NLS-GI-VP16-IRES-NLS-GAL4DBD-GID-SmBiT-FKF1

Plasmid

#183404

Purpose

Dual expression of GI-VP16 and GAL4DBD-GID-SmBiT-FKF1 in the nucleus

Depositor

Alice Ting

Article

Erdenee et al ACS Synth Biol. 2022 Mar 18;11(3):1086-1

Insert

GI-VP16-IRES-GAL4DBD-GID-SmBiT-FKF1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
NLS-GI-VP16-IRES-NLS-GAL4DBD-NanoLuc-FKF1

Plasmid

#183399

Purpose

Dual expression of GI-VP16 and GAL4DBD-NanoLuc-FKF1 in the nucleus

Depositor

Alice Ting

Article

Erdenee et al ACS Synth Biol. 2022 Mar 18;11(3):1086-1

Insert

GI-VP16-IRES-GAL4DBD-NanoLuc-FKF1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS105

Plasmid

#174830

Purpose

Encodes a unique yeast telomere end at TEL11R by removing the subtelomere

Depositor

Carol Greider

Article

Sholes et al Genome Res. 2022 Apr;32(4):616-628. doi:

Insert

11R Unique

Use

Tags

GAL4 5XUAS

Expression

Bacterial

Mutation

Changing TEL11R to a unique end

Promoter

lac promoter

Available since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
TOMM20-mTurquoise-RhoG-QL-deltaCaaX

Plasmid

#176122

Purpose

Constitutively active, mitochondiral localized RhoG

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

RHOG (RHOG Human)

Use

Tags

Expression

Mammalian

Mutation

QL

Promoter

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 2921 - 2960 of 13524 results

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