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pmScarlet-HSP70

Plasmid

#163790

Purpose

Expresses mScarlet-Hsp70 in mammalian cells

Depositor

Vincent Timmerman

Article

Adriaenssens et al Sci Rep. 2020 May 29;10(1):8755. doi: 10

Insert

HSP70 (HSPA1A Human)

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pKC152_Lenti_tetoff_PABP-INT_EF1a_EGFP

Plasmid

#250950

Purpose

A lentiviral vector express PABP-INT fusion protein under a Tetoff system. Contains EGFP as selection marker.

Depositor

Fei Chen

Article

Chao et al Science. 2026 Jan 15:eadz9353. doi: 10.1

Insert

PABPC1 (PABPC1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE3G

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag CKM

Plasmid

#20458

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

CKM (CKM Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pGEX-4T-1 3xFlag-CREB

Plasmid

#82721

Purpose

Expresses human CREB

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

CREB (CREB1 Human)

Tags

3XFLAG and GST

Expression

Bacterial

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pNP2322 [Cloning backbone, mCherry Inversion Activity Reporter]

Plasmid

#247250

Purpose

Cloning backbone for measuring bridge recombinase mediated inversion

Depositor

Patrick Hsu

Article

Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-HA-L2HGDH(opt)-FLAG

Plasmid

#230089

Purpose

expressing human L2HGDH

Depositor

Xingguo Zhu

Article

Xi et al Commun Biol. 2025 Jan 6;8(1):15. doi: 10

Insert

L2HGDH (L2HGDH Human)

Use

Lentiviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Multiple wobble mutations

Promoter

CMV

Available Since

Jan. 10, 2025

Availability

Academic Institutions and Nonprofits only

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tetO-MITF

Plasmid

#170695

Purpose

doxycycline-inducible overexpression of MITF

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

MITF (MITF Human)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Promoter

TRE promoter, Tet-ON

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pEF-BFP

Plasmid

#138272

Purpose

Expression of engineered BFP variant that undergoes conversion to GFP after targeted modification with a cytidine deaminase-based DNA base editor

Depositor

Xiao Wang

Article

Standage-Beier et al Nucleic Acids Res. 2019 Aug 20. pii: 555

Insert

BFP H66 PAM

Use

CRISPR

Expression

Mammalian

Mutation

H66, PAM motif

Promoter

Ef1a

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-ADGRL3-Flag

Plasmid

#201218

Purpose

pCDNA3.1 with kozak (GCC), ADGRL3 and C-terminal Flag

Depositor

Jonathan Javitch

Article

Perry-Hauser et al J Biol Chem. 2022 Dec;298(12):102594. do

Insert

ADGRL3 (Adgrl3 Mouse)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-Mito-7

Plasmid

#55102

Purpose

Localization: Mitochondria, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Article

Olenych et al Curr Protoc Cell Biol. 2007 Sep;Chapter

Insert

Mito (COX8A Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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mCherry-NUP50-N-10

Plasmid

#55111

Purpose

Localization: Nuclear Pore, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

NUP50 (NUP50 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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FH-Tet2-pEF

Plasmid

#41710

Purpose

This plasmid expresses mouse Tet2 with N-terminal FLAG and HA tags in a mammalian system

Depositor

Anjana Rao

Article

Ko et al Nature. 2010 Dec 9;468(7325):839-43. doi

Insert

tet methylcytosine dioxygenase 2 (Tet2 Mouse)

Tags

FLAG and HA

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pEF1α-Cre-2A-mTagBFP2

Plasmid

#207746

Purpose

Expression of the Cre recombinase linked to a mTagBFP2 fluorescent marker

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

Cre-PGK-mTagBFP2

Use

Cre/Lox

Expression

Mammalian

Promoter

EF1alpha

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pCS2FA CO Tol2 TPase

Plasmid

#133032

Purpose

Expression vector for production of codon optimized Tol2 transposase

Depositor

Charles Nathan Hancock

Article

Mackey et al MicroPubl Biol. 2020 Jun 11;2020:10.1791

Insert

Codon optimized Tol2 Transposase

Use

In vitro expression

Mutation

Codon Optimized

Available Since

June 15, 2020

Availability

Academic Institutions and Nonprofits only

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pLV_3xFLAG-ATP6V0A3-mScarlet

Plasmid

#228867

Purpose

Stable expression of V-ATPase subunit ATP6V0A3 with N-terminal 3xFLAG and C-terminal mScarlet

Depositor

Wilhelm Palm

Article

Ratto et al Nat Commun. 2022 Aug 17;13(1):4848. doi:

Insert

ATP6V0A3 (TCIRG1 Human)

Use

Lentiviral

Tags

3xFLAG and mScarlet

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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PrimeRoot.v2C-Cre

Plasmid

#201739

Purpose

For DNA insertion test in protoplast

Depositor

Caixia Gao

Article

Sun et al Nat Biotechnol. 2023 Apr 24. doi: 10.103

Insert

ePPE,Cre

Use

CRISPR

Expression

Plant

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1-Luc2-P2A-tdTomato

Plasmid

#72486

Purpose

Express Luc2 and tdTomato under EF1 promoter

Depositor

Kazuhiro Oka

Insert

Luc2-P2A-tdTomato

Use

Lentiviral

Expression

Mammalian

Promoter

EF1

Available Since

Jan. 11, 2016

Availability

Academic Institutions and Nonprofits only

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Lenti-luciferase-P2A-Neo

Plasmid

#105621

Purpose

3rd generation lentiviral plasmid for expression of luciferase with Neo resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

Luciferase

Use

Lentiviral and Luciferase

Expression

Mammalian

Promoter

EFS

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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ppyCAG_RNaseH1_WT

Plasmid

#111906

Purpose

Express RNASEH1 in mammalian cell.

Depositor

Xiang-Dong Fu

Article

Chen et al Mol Cell. 2017 Nov 16;68(4):745-757.e5.

Insert

RNASEH1 (RNASEH1 Human)

Tags

V5

Expression

Mammalian

Available Since

July 4, 2018

Availability

Academic Institutions and Nonprofits only

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pSCD-Casp3-L-tev-S

Plasmid

#226230

Purpose

Express TEVP-cleavable caspase-3

Depositor

Michael Elowitz

Article

Xia et al Cell. 2024 Apr 16:S0092-8674(24)00347-7.

Insert

Caspase-3 (CASP3 Human)

Expression

Mammalian

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pBMN FKBP(DD)-YFP

Plasmid

#31763

Depositor

Thomas Wandless

Article

Banaszynski et al Cell. 2006 Sep 8;126(5):995-1004.

Insert

FKBP(DD)-YFP-HA (FKBP1A Human)

Use

Retroviral

Tags

HA and YFP

Mutation

F36V, L106P on FKBP12

Promoter

LTR

Available Since

Oct. 3, 2011

Availability

Academic Institutions and Nonprofits only

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pICE-EGFP-FLAG-PSMD2

Plasmid

#161917

Purpose

Expresses human PSMD2 with a N-terminal GFP-FLAG tag. Confers Puromycin resistance. Inducible in T-REx cells.

Depositor

Sébastien Britton

Article

Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

Insert

26S proteasome non-ATPase regulatory subunit 2 (PSMD2 Human)

Tags

GFP-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-EGFP-LAMP2

Plasmid

#222321

Purpose

Synchronize the trafficking of LAMP2 from the ER.

Depositor

Franck Perez

Article

Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

Insert

Streptavidin-KDEL and LAMP2 fused to SBP-EGFP (LAMP2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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TFORF2029

Plasmid

#143566

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

SMARCA5 (SMARCA5 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pEGFP‐RNASEH2B

Plasmid

#108697

Purpose

For expression of N-terminally eGFP-tagged human RNASEH2B in mammalian cells

Depositor

Andrew Jackson, Martin Reijns

Article

Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

Insert

RNASEH2B (RNASEH2B Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pMK467 (dTAG-BromoTag-mAID-EGFP)

Plasmid

#214393

Purpose

dTAG-BromoTag-mAID-EGFP reporter (TOL2)

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

dTAG-BromoTag-mAID-EGFP reporter

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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PX458-mcherry

Plasmid

#161974

Purpose

Expresses Cas9-p2a-mcherry (BbsI site silenced) and sgRNA

Depositor

Joanna Wysocka

Article

Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

Insert

Cas9-t2a-mcherry

Use

CRISPR

Expression

Mammalian

Promoter

Cbh

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pKC148_Lenti_Tetoff_MVP_EF1a_tagRFP

Plasmid

#250949

Purpose

A lentiviral vector express MVP gene under a Tetoff system. Contains tagRFP as selection marker.

Depositor

Fei Chen

Article

Chao et al Science. 2026 Jan 15:eadz9353. doi: 10.1

Insert

MVP (MVP Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE3G

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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CD3 delta YFP

Plasmid

#11951

Depositor

Nico Dantuma

Article

Menendez-Benito et al Hum Mol Genet. 2005 Oct 1. 14(19):2787-9

Insert

CD3delta-YFP

Expression

Mammalian

Available Since

June 22, 2006

Availability

Academic Institutions and Nonprofits only

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pFL-SV40

Plasmid

#115352

Purpose

firefly luciferase (Fluc) reporter gene driven by SV40 promoter

Depositor

Ming-Chih Lai

Article

Lai et al Mol Biol Cell. 2008 Sep;19(9):3847-58. d

Type

Empty backbone

Use

Luciferase

Expression

Mammalian

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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