161,439 results

1. pCMV4 p65

   Plasmid

   #21966

   Depositor

   Warner Greene

   Article

   Ballard et al Proc Natl Acad Sci U S A. 1992 Mar 1. 89

   Insert

   NFkB p65 subunit (RELA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   This construct was the product of 3 subcloning pr…

   Promoter

   Available since

   Sept. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. Flag-hPIASy

   Plasmid

   #15208

   Depositor

   Ke Shuai

   Article

   Liu et al Proc Natl Acad Sci U S A. 2001 Mar 13. 9

   Insert

   PIASy (PIAS4 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p23-NES-EsCas13d-msfGFP-NES-Flag

   Plasmid

   #165074

   Purpose

   overexpression of EsCas13d in human cells

   Depositor

   Ling-Ling Chen

   Article

   Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

   Insert

   EsCas13d
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMasterK

   Plasmid

   #165081

   Purpose

   Episomal expression of reprogramming factors.

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al Stem Cell Res. 2021 May;53:102380. doi:

   Inserts

   OCT4 (POU5F1 Human)
   SOX2 (SOX2 Human)
   KLF4 (KLF4 Human)
   MYC (MYC Human)
   LRH1 (NR5A2 Human)
   NANOG (NANOG Human)
   lin28 (LIN28A Human)
   EGFP
   HSV TK
   KDM4D (KDM4D Callithrix jacchus)
   GLIS1 (GLIS1 Human)
   

   Use

   Episomal

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJKR-H-benM

   Plasmid

   #73925

   Purpose

   Muconate biosensor. Expresses GFP proportional to muconate concentration within the cell.

   Depositor

   George Church

   Article

   Rogers et al Proc Natl Acad Sci U S A. 2016 Feb 8. pi

   Insert

   muconate biosensing apparatus
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBK-CMV Pig12

   Plasmid

   #16506

   Depositor

   Bert Vogelstein

   Article

   Polyak et al Nature. 1997 Sep 18. 389(6648):300-5.

   Insert

   Pig12 (PTGES Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

7. pTol2-Huc-Positron-ST

   Plasmid

   #129265

   Purpose

   pan neuronal expression of soma-localized Positron in zebrafish

   Depositor

   Eric Schreiter

   Article

   Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

   Insert

   Positron-ST
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   D92N, E199V

   Promoter

   Available since

   Aug. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOTTC352 - pAAV CaMKIIa eNpHR3.0-iRFP

   Plasmid

   #47904

   Purpose

   An AAV vector that expresses engineered halorhodopsin 3.0 (fused to iRFP) under the CaM Kinase IIa promoter.

   Depositor

   Brandon Harvey

   Article

   Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

   Insert

   eNpHR3.0
   

   Use

   AAV

   Tags

   iRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKIIa

   Available since

   Sept. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET15b-RvLEAMshort

   Plasmid

   #218122

   Purpose

   Expresses truncated RvLEAM protein under IPTG induction in bacterial host cells.

   Depositor

   Ci Ji Lim

   Article

   Abe et al bioRxiv [Preprint]. 2024 Feb 11:2024.02.

   Insert

   RvLEAMshort
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Truncated form (58-181aa)

   Promoter

   T7

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSEMS-Halo7Tag-hFis

    Plasmid

    #111136

    Purpose

    hFis with selflabeling tag, e.g. for fluorescence microscopy

    Depositor

    Karin Busch

    Article

    Appelhans et al Nano Lett. 2012 Feb 8;12(2):610-6. doi:

    Insert

    human fission factor1 (FIS1 Human)
    

    Use

    Tags

    Halo7-tag

    Expression

    Mammalian

    Mutation

    *

    Promoter

    CMV

    Available since

    July 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCBh_NLS_hfCas13X(Cas13X_M17YY)_NLS-pA-U6-DR-BpiI-BpiI-DR-pSV40-EGFP-pA-pSV40-mCherry-pA

    Plasmid

    #190033

    Purpose

    vector for encoding a human codon-optimized High-fidelity Cas13X (hfCas13X) driven by CBh promoter, guide RNAs compatible with Cas13X driven by hU6, EGFP and mCherry driven by SV40 promoters

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

    Insert

    humanized hfCas13X
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Y672A,Y676A

    Promoter

    CBh, SV40, hU6

    Available since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-GenEPi

    Plasmid

    #140236

    Purpose

    Mammalian expression of Piezo1-based fluorescent force sensor GenEPi

    Depositor

    Periklis Pantazis

    Article

    Yaganoglu et al Nat Commun. 2023 Jul 19;14(1):4352. doi:

    Insert

    GenEPi (Piezo1-based fluorescent force sensor) (PIEZO1 Human)
    

    Use

    Tags

    GCaMP 6s RS1 EF4

    Expression

    Mammalian

    Mutation

    Promoter

    simian CMV IE94

    Available since

    July 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLAMP1-miRFP670nano

    Plasmid

    #127435

    Purpose

    LAMP1 (Lysosome Membrane) labeling with near-infrared fluorescent protein miRFP670nano

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

    Insert

    LAMP1-miRFP670nano
    

    Use

    Tags

    miRFP670nano

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Flag-hPIASx alpha

    Plasmid

    #15209

    Depositor

    Ke Shuai

    Article

    Arora et al J Biol Chem. 2003 Jun 13. 278(24):21327-

    Insert

    PIASx alpha (PIAS2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

15. PRExpress

    Plasmid

    #122486

    Purpose

    A non-leaky heat-inducible gene expression system for Drosophila. (GenBank: MF058598.1)

    Depositor

    Renato Paro

    Article

    Akmammedov et al Sci Rep. 2017 Jul 31;7(1):6899. doi: 10.

    Inserts

    LacZ
    poly-PRE
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDH-EF1a-eFFly-mCherry

    Plasmid

    #104833

    Purpose

    3rd generation lentiviral vector. eFFly luciferase for in vivo Imaging, mCherry for fluorescent detection

    Depositor

    Irmela Jeremias

    Article

    Ebinger et al Cancer Cell. 2016 Dec 12;30(6):849-862.

    Inserts

    eFFly
    mCherry
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pmGFP-ADAR1-p150

    Plasmid

    #117927

    Purpose

    ADAR1-p150 expression plasmid.

    Depositor

    Kumiko Ui-Tei

    Article

    Galipon et al Genes (Basel). 2017 Feb 11;8(2). pii: ge

    Insert

    ADAR1-p150 (ADAR Human)
    

    Use

    Tags

    myc-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mVenus-N1

    Plasmid

    #54640

    Purpose

    Localization: N1 Cloning Vector, Excitation: 515, Emission: 528

    Depositor

    Atsushi Miyawaki, Michael Davidson

    Article

    Nagai et al Nat Biotechnol. 2002 Jan;20(1):87-90.

    Type

    Empty backbone

    Use

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

19. p3259 pCMV hUBC9 mt HA

    Plasmid

    #14439

    Depositor

    Peter Howley

    Article

    Yasugi et al Nucleic Acids Res. 1996 Jun 1. 24(11):20

    Insert

    UBC9 C93S (UBE2I Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Cys93Ser mutation (TGC changed to AGC)

    Promoter

    CMV

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDA007

    Plasmid

    #131380

    Purpose

    ORFeus expression, WT

    Depositor

    Kathleen Burns

    Article

    Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

    Insert

    ORFeus-Hs
    

    Use

    Tags

    ORF2-3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. LgBit-NbV5

    Plasmid

    #201476

    Purpose

    NanoBit detection of V5-tagged protein

    Depositor

    Patrick Giguère

    Article

    Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

    Insert

    LgBit-NbV5
    

    Use

    Tags

    LgBit

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. AiP14084 - pAAV-mMbp_promoter-SYFP2-P2A-3XFLAG-10aa-H2B*-WPRE3-BGHpA (Alias: CN4084)

    Plasmid

    #208160

    Purpose

    Direct-expressing SYFP2 in oligodendrocytes AAV Virus

    Depositor

    The Allen Institute for Brain Science, Boaz Levi

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    Beta Globin minimal promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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23. MazF-WT

    Plasmid

    #155202

    Purpose

    To express recombinant MazF protein.

    Depositor

    Guanzheng Luo

    Article

    Zhang et al Sci Adv. 2019 Jul 3;5(7):eaax0250. doi:

    Insert

    MazF
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3-TrxRFP2

    Plasmid

    #172322

    Purpose

    an improved fluorescent biosensor for thioredoxin redox

    Depositor

    Huiwang Ai

    Article

    Pang et al Biochemistry. 2022 Mar 1;61(5):377-384.

    Insert

    TrxRFP2, an improved version from TrxRFP1
    

    Use

    Tags

    His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pREXC3GH

    Plasmid

    #47077

    Purpose

    FX cloning Lactococcus lactis expression vector (VBEx) with nisin promoter and C-terminal 3C protease cleavage site, GFP and 10x His tags

    Depositor

    Raimund Dutzler, Eric Geertsma

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Use

    Lactococcus lactis expression

    Tags

    3C protease site, GFP, and His

    Expression

    Mutation

    Promoter

    NIS (nisin)

    Available since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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26. AGTR2-Tango

    Plasmid

    #66223

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    AGTR2 (AGTR2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

27. pcDNA3-PTK7-VSV

    Plasmid

    #65250

    Purpose

    Expression of VSV tagged PTK7 in mammalian cells

    Depositor

    Axel Ullrich

    Article

    Gartner et al PLoS One. 2014 Jan 7;9(1):e84472. doi: 1

    Insert

    PTK7 (PTK7 Human)
    

    Use

    Tags

    VSV

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pECGMC3

    Plasmid

    #75441

    Purpose

    Plasmid for mf-lon mediated mCherry degradation in Lactococcus lactis. Contains mcherry-pdt3

    Depositor

    James Collins

    Article

    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

    Insert

    mf-lon
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    nisA promoter

    Available since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Flag-mPIAS3

    Plasmid

    #15207

    Depositor

    Ke Shuai

    Article

    Chung et al Science. 1997 Dec 5. 278(5344):1803-5.

    Insert

    PIAS3 (Pias3 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    V580I

    Promoter

    Available since

    Feb. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCS2-epNTR

    Plasmid

    #62213

    Purpose

    epNTR (zebrafish optimized nitroreductase, T41L, N71S, F124W) in pCS2

    Depositor

    Harold Burgess

    Article

    Tabor et al J Neurophysiol. 2014 Aug 15;112(4):834-4

    Insert

    epNTR
    

    Use

    Mrna synthesis

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge