159,238 results

1. pcDNA3.1_ 2xFLAG-2xSTREP_BACH1

   Plasmid

   #159130

   Purpose

   expresses BACH1 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

   Insert

   BACH1 (BACH1 Human)
   

   Use

   Tags

   2X-FLAG _ 2X-STREP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. Dual-sgRNA_hU6-mU6

   Plasmid

   #154194

   Purpose

   Lentiviral expression plasmid for two sgRNAs from a hU6 and a mU6 promoter. The cloning site design allows a one-step cloning strategy of both sgRNAs. Expresses a Thy1.1 marker.

   Depositor

   Anna Obenauf

   Article

   Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. RFP-ATF4

   Plasmid

   #125237

   Purpose

   ATF4 expression vector

   Depositor

   Rozita Yarmand

   Article

   Bagheri-Yarmand et al J Biol Chem. 2015 May 1;290(18):11749-61

   Insert

   ATF4 (ATF4 Human)
   

   Use

   Tags

   RFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lenti-CMV-PEmax-IRES-ZeoR

   Plasmid

   #207353

   Purpose

   Lentiviral transfer plasmid containing CMV-driven expression cassette encoding PEmax enzyme for prime editing.

   Depositor

   Marianne Carlon

   Article

   Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

   Insert

   Prime editor max (PEmax) enzyme
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDualIN-SMN2

   Plasmid

   #214430

   Purpose

   Dual-color reporter express eGFP constitutively; tdTomato expression is contingent on the inclusion of the frame-shifting cassette exon 7

   Depositor

   Chaolin Zhang

   Article

   Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

   Insert

   SMN2 EXON 7 (SMN2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. 1GFP/RNase H1 D210N

   Plasmid

   #174448

   Purpose

   Express GFP-tagged RNase H1 D210N in E. coli.

   Depositor

   Karlene Cimprich

   Article

   Crossley et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

   Insert

   RNase H1 (RNASEH1 Human)
   

   Use

   Tags

   His-tag, GFP

   Expression

   Bacterial

   Mutation

   D210N

   Promoter

   T7 promoter

   Available since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. Flag-TRIM24

   Plasmid

   #28138

   Depositor

   Michelle Barton

   Article

   Allton et al Proc Natl Acad Sci U S A. 2009 Jul 14. 1

   Insert

   TRIM24 (TRIM24 Human)
   

   Use

   Tags

   FLAGX2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA3.3 N-MYC TIP60

   Plasmid

   #213003

   Purpose

   expresses human TIP60

   Depositor

   Sheng-cai Lin

   Article

   Wu et al Cell Res. 2023 Nov;33(11):835-850. doi:

   Insert

   KAT5 (KAT5 Human)
   

   Use

   Tags

   MYC

   Expression

   Mammalian

   Mutation

   Promoter

   cmv

   Available since

   Feb. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. EGFP-LC3

   Plasmid

   #11546

   Depositor

   Karla Kirkegaard

   Article

   Jackson et al PLoS Biol. 2005 May . 3(5):e156.

   Insert

   LC3B (MAP1LC3B Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA-dCas9-p300 Core

    Plasmid

    #61357

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, S. pyogenes, Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    CMV

    Available since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-C1-AR Q48

    Plasmid

    #86428

    Purpose

    Fluorescent human androgen receptor (fused to EGFP) with expanded polyglutamine region

    Depositor

    Michael Mancini

    Article

    Stenoien et al Hum Mol Genet. 1999 May . 8(5):731-41.

    Insert

    Androgen receptor (AR) (AR Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Poly-glutamine expansion, G130D

    Promoter

    CMV

    Available since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBR.DT-A

    Plasmid

    #35955

    Depositor

    Jacqueline Lees

    Article

    Danielian et al Oncogene. 2008 Nov 20;27(51):6561-70. Ep

    Insert

    diphtheria toxin A
    

    Use

    Bac recombineering

    Tags

    Expression

    Mutation

    Promoter

    PGK

    Available since

    April 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. EGFP-GRAM-H

    Plasmid

    #211700

    Purpose

    Expression of EGFP-tagged GRAM domain of GRAMD1b carrying a G187L mutation (EGFP-GRAM1b G187L) in mammalian cells

    Depositor

    Yasunori Saheki

    Article

    Ercan et al EMBO J. 2021 Mar 15;40(6):e106524. doi:

    Insert

    GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Changed Glycine 187 to Leucine

    Promoter

    CMV

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pF2-DTA

    Plasmid

    #42521

    Depositor

    Terry Magnuson

    Article

    Jang et al PLoS One. 2013;8(2):e57075. doi: 10.1371

    Insert

    EF1a-DTA
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCW57.1-MAT2A

    Plasmid

    #100521

    Purpose

    Dox-repressible cDNA expression of MAT2A (dox-off/ tet-off all-in-one vector)

    Depositor

    David Sabatini

    Article

    Gu et al Science. 2017 Nov 10;358(6364):813-818.

    Insert

    MAT2A (MAT2A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pTight TRE

    Available since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. MIG-Luciferase-IRES-GFP

    Plasmid

    #75021

    Purpose

    Encodes the expression of Luciferase and GFP

    Depositor

    Saradhi Mallampati

    Insert

    Luciferase
    

    Use

    Luciferase and Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-hSyn-ASAP3-WPRE

    Plasmid

    #132331

    Purpose

    expresses ASAP3(ASAP-family genetically encoded voltage indicator) in mammalian cells, gene delivered by pAAV-hSyn vector

    Depositor

    Michael Lin

    Article

    Villette et al Cell. 2019 Dec 12;179(7):1590-1608.e23.

    Insert

    ASAP3
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin

    Available since

    Dec. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDEST53-LRRK2-WT

    Plasmid

    #25044

    Depositor

    Mark Cookson

    Article

    Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEGFP-C1-AR Q0

    Plasmid

    #86427

    Purpose

    Fluorescent human androgen receptor (fused to EGFP) with deleted polyglutamine region

    Depositor

    Michael Mancini

    Article

    Stenoien et al Hum Mol Genet. 1999 May . 8(5):731-41.

    Insert

    androgen receptor (AR) (AR Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Deletion of the poly-glutamine expansion

    Promoter

    CMV

    Available since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. P3-dCas9-Tet1-GFP-Puro

    Plasmid

    #190728

    Purpose

    Expression of human spdCas9-Tet1 fusion for targeted DNA methylation in mammalian cell. EGFP-puromycin double marker under an independent CMV promoter. Cloning backbone for spgRNA (BbsI)

    Depositor

    Maja Jagodic

    Article

    Pahlevan Kakhki et al Nat Commun. 2024 Jul 30;15(1):6419. doi:

    Insert

    dCas9-tet1
    

    Use

    CRISPR

    Tags

    3XFLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    Changed Pro434 codon from CCG to CCC and Gly468 f…

    Promoter

    pCAG

    Available since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. pLenti-Cas9-GFP

    Plasmid

    #86145

    Purpose

    Lentiviral vector expressing Cas9-GFP

    Depositor

    David Sabatini

    Article

    Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

    Insert

    Cas9-GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Feb. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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22. sUPRa

    Plasmid

    #223242

    Purpose

    sUPRa is a dual promoter and two-color construct, which is designed to report the global unfolded protein response (UPR) and affords unbiased cell detection irrespective of UPR levels

    Depositor

    Colin Akerman

    Article

    Chakrabarty et al Sci Rep. 2024 Jul 1;14(1):14990. doi: 10

    Insert

    sUPRa
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Custom

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. GFP-SMN

    Plasmid

    #37057

    Depositor

    Greg Matera

    Article

    Shpargel et al Proc Natl Acad Sci U S A. 2005 Nov 29;10

    Insert

    Human SMN1 (SMN1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. LM627

    Plasmid

    #191551

    Purpose

    LM627_MSG-AGGA-promoter-ccdb-TTCC-SNAC-his

    Depositor

    David Baker

    Article

    Wicky et al Science. 2022 Oct 7;378(6615):56-61. doi

    Insert

    ccdb
    

    Use

    Tags

    SNAC-tag, HIS-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCMV-hARdelta538-614

    Plasmid

    #89107

    Purpose

    mammalian expression of human androgen receptor with deletion of amino acids 538-614, deletion of AR DNA binding domain

    Depositor

    Elizabeth Wilson

    Article

    Zhou et al J Biol Chem. 1994 May 6;269(18):13115-23

    Insert

    hAR-538-614 (AR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    hAR delta 538-614

    Promoter

    CMV

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

26. pSMALB-ATF4.5

    Plasmid

    #155032

    Purpose

    Fluorescent reporter for ATF4 translation

    Depositor

    Peter van Galen, John Dick

    Article

    van Galen et al Nature. 2014 Jun 12;510(7504):268-72. do

    Inserts

    ATF4 5' UTR (Atf4 Mouse)
    ATF4-eGFP fusion ORF
    TagBFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMXs-IP- SCAT3.2

    Plasmid

    #172497

    Purpose

    SCAT3.2 is fluorescent reporter (FRET probe) for cell survival/apoptosis with Caspase-3 sensitive sequence which can be introduced to mammalian cells by retrovirus vector.

    Depositor

    David Scadden

    Article

    Oki et al Nat Commun. 2021 Jan 11;12(1):245. doi:

    Insert

    Amcyan
    

    Use

    Retroviral

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    MLV LTR

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCMV-hARdeltaNH2-6

    Plasmid

    #89104

    Purpose

    mammalian expression of human androgen receptor amino acids 507-919

    Depositor

    Elizabeth Wilson

    Article

    Simental et al J Biol Chem. 1991 Jan 5;266(1):510-8.

    Insert

    hAR DNA binding domain+ligand binding domain (AR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NH2 terminal deletion, contains amino acids 507-…

    Promoter

    CMV

    Available since

    April 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSin-EF2-Sox2-Pur

    Plasmid

    #16577

    Depositor

    James Thomson

    Article

    Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

    Insert

    Homo sapiens SRY (sex determining region Y)-box 2 (SOX2) (SOX2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 4, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCDNA3.1(+)-NEMOf

    Plasmid

    #189932

    Purpose

    Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity

    Depositor

    Yubin Zhou

    Article

    Li et al Nat Methods. 2023 Jun;20(6):918-924. doi

    Insert

    NEMOf
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge