166,778 results

1. pWN_U6-TRAC-miniGag-Cas9

   Plasmid

   #228959

   Purpose

   miniEDV production plasmid. Expresses the miniGag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter)

   Depositor

   Jennifer Doudna

   Article

   Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

   Insert

   miniGag-Cas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Jan. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pInducer10b-EGFP-KRAS G12V

   Plasmid

   #164925

   Purpose

   Expresses EGFP cDNA and KRAS G12V cDNA in mammalian cells

   Depositor

   Ji Luo

   Article

   Carver et al PLoS One. 2014 Aug 5;9(8):e103836. doi:

   Inserts

   Enhanced green fluorescent protein
   KRAS proto-oncogene, GTPase (KRAS) G12V mutant (KRAS Human)
   

   Use

   Lentiviral

   Mutation

   Glycine 12 changed to valine (G->T 3156)

   Promoter

   Ubc Promoter and Ubc promoter

   Available Since

   May 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. DYRK1A_pCSdest

   Plasmid

   #53791

   Depositor

   Roger Reeves

   Article

   Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

   Insert

   DYRK1A (DYRK1A Human)
   

   Expression

   Mammalian

   Promoter

   SP6 and CMV

   Available Since

   Sept. 8, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. EGFR-FR

   Plasmid

   #179263

   Purpose

   Human EGFR C-terminally labeled with FusionRed

   Depositor

   Jared Toettcher

   Article

   Farahani et al Cell Rep. 2021 Dec 28;37(13):110181. doi

   Insert

   EGFR (EGFR Human)
   

   Use

   Lentiviral

   Tags

   FusionRed

   Promoter

   SFFV

   Available Since

   Feb. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRetro-CAG-DsRedExpress-TVA

   Plasmid

   #235699

   Purpose

   Control plasmid expressing TVA and DsRed without G protein

   Depositor

   Guo-li Ming, Hongjun Song

   Article

   Sun et al Nature. 2025 Jan 16. doi: 10.1038/s41586

   Insert

   DsRedExpress, TVA800
   

   Use

   Retroviral

   Promoter

   CAG

   Available Since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCI-Puro_hCOL4A4-Myc-His

   Plasmid

   #198093

   Purpose

   Express hCOL4A4 in mammalian cells

   Depositor

   Jeffrey Miner

   Insert

   COL4A4 (COL4A4 Human)
   

   Tags

   TEV-Myc-His

   Expression

   Mammalian

   Promoter

   CMV, beta-globin/IgG chimeric intron

   Available Since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. HaloTag-LaminA

   Plasmid

   #231894

   Purpose

   Expression of HaloTag-tagged LaminA to visualize the nuclear lamina

   Depositor

   Lukas Kapitein

   Article

   Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

   Insert

   LaminA (LMNA Human)
   

   Tags

   HaloTag

   Expression

   Mammalian

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pChillyBoys_gen1

   Plasmid

   #228495

   Purpose

   pACYCDuet-1 encoding Oleispira antarctica GroEL and GorES low temp chaperonins for enhanced low-temperature induction (E. coli based protein expression)

   Depositor

   Richard Baker

   Inserts

   groEL
   groES
   

   Expression

   Bacterial

   Promoter

   T7 Promoter

   Available Since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pClneoMyc-LATS1

   Plasmid

   #66851

   Purpose

   Express Myc-tagged LATS1 in mammalian cells

   Depositor

   Yutaka Hata

   Article

   Bao et al J Biochem. 2011 Aug;150(2):199-208. doi:

   Insert

   LATS1 (LATS1 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   P44L mutation in LATS1 compared to GenBank refere…

   Promoter

   CMV

   Available Since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJT258 Lenti pEF-dCas9-3XFLAG-N+F+Z-T2A-tagBFP-P2A-Blast

    Plasmid

    #187334

    Purpose

    dCas9 effector fusion for manipulating transcription

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    dCas9-3XFLAG-N+F+Z-T2A-tagBFP-P2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO.3G

    Plasmid

    #14748

    Depositor

    Christophe Benoist, Diane Mathis

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    April 5, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAF0036 Ef1a-Si74-2a-EGFP

    Plasmid

    #193463

    Purpose

    Expression of Si74 recombinase from Ef1a promoter

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Si74
    

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLentiCRISPRv2_mSTING_scrambled_gRNA_1

    Plasmid

    #196627

    Purpose

    Scrambled control 1 for STING knock-out in murine cells.

    Depositor

    Nicolas Manel

    Article

    Jneid et al Sci Immunol. 2023 Jan 13;8(79):eabn6612.

    Insert

    mSTING scrambled gRNA 1
    

    Use

    CRISPR and Lentiviral

    Mutation

    WT

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pH6HTC_ENO1-Halo

    Plasmid

    #175330

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    ENO1 (ENO1 Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Available Since

    Dec. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. TFORF2538

    Plasmid

    #144036

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SOX4 (SOX4 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-CAG-iGluSnFR4s-NGR-WPRE

    Plasmid

    #234451

    Purpose

    AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4s-NGR
    

    Use

    AAV

    Tags

    IgK chain and Myc epi tag-NGR GPI anchor

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-EFS-DIO-GRAB_Ado1.0

    Plasmid

    #153291

    Purpose

    Express the adenosine sensor GRAB_Ado1.0 in Cre positive cells

    Depositor

    Yulong Li

    Article

    Peng et al Science. 2020 Sep 4;369(6508). pii: 369/

    Insert

    Adenosine sensor GRAB_Ado1.0
    

    Use

    AAV

    Promoter

    EFS

    Available Since

    July 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-LacR-TopBP1

    Plasmid

    #31317

    Depositor

    Aziz Sancar

    Article

    Lindsey-Boltz et al J Biol Chem. 2011 Jun 3. 286(22):19229-3

    Insert

    TopBP1 (TOPBP1 Human)
    

    Tags

    FLAG and LacR

    Expression

    Mammalian

    Available Since

    Nov. 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pWUR790

    Plasmid

    #101723

    Purpose

    Synthetic codon optimized P. furiosus ago with N-terminal Strep(II)-tag in pCDF-1b vector, expression vector for PfAgo

    Depositor

    John van der Oost

    Article

    Swarts et al Nucleic Acids Res. 2015 May 26;43(10):51

    Insert

    PfAgo
    

    Tags

    Strep(II)-tag

    Expression

    Bacterial

    Promoter

    T7 Promotor

    Available Since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDONR221_SLC16A3

    Plasmid

    #131899

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC16A3 (SLC16A3 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 11, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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21. pTIPI_mIGg2a_Anti-HA [12CA5]

    Plasmid

    #210656

    Purpose

    Mammalian expression of Anti-HA [12CA5] mouse IgG2a heavy chain; to be used with pTIPI_mK_Anti-HA [12CA5] light chain (Plasmid 210657) to make the antibody.

    Depositor

    Melina Fan, Addgene Research Program

    Insert

    Anti-HA [12CA5] mouse IgG2a heavy chain
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Mouse IgG2a heavy chain

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 18, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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22. pTIPI_mK_Anti-HA [12CA5]

    Plasmid

    #210657

    Purpose

    Mammalian expression of Anti-HA [12CA5] mouse kappa light chain; to be used with pTIPI_mIGg2a_Anti-HA [12CA5] heavy chain (Plasmid 210656) to make the antibody.

    Depositor

    Melina Fan, Addgene Research Program

    Insert

    Anti-HA [12CA5] kappa light chain
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Mouse kappa light chain

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 18, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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23. pTKEI-Tre-C04

    Plasmid

    #79754

    Purpose

    Single fluorescent protein biosensor for trehalose

    Depositor

    David Savage

    Article

    Nadler et al Nat Commun. 2016 Jul 29;7:12266. doi: 10

    Insert

    Tre-C04
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    pTrc

    Available Since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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24. miReporter-CAG

    Plasmid

    #82478

    Purpose

    microRNA reporter relying on a bidirectional CAG-based promoter. Contains MCS downstream of H2B-mCherry and H2B-Citrine to insert miRNA binding sites. Contains a Hygromycin selection cassette.

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2016 May 6;11(5):e0155177. doi

    Insert

    H2B-mCherry and H2B-Citrine. HygroR
    

    Available Since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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25. pAAV-hSyn-DIO-GRAB_ACh3.0

    Plasmid

    #121923

    Purpose

    express the genetically-encoded fluorescent acethycholine(ACh) sensor GRAB-ACh3.0 in a cre-dependent manner

    Depositor

    Yulong Li

    Article

    Jing et al Nat Methods. 2020 Sep 28. pii: 10.1038/s

    Insert

    GPCR activation based ACh sensor GRAB-ACh3.0
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Jan. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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26. pYTK072

    Plasmid

    #65179

    Purpose

    Encodes ConRE as a Type 5 part to be used in the Dueber YTK system

    Depositor

    John Dueber

    Article

    Lee et al ACS Synth Biol. 2015 Apr 14.

    Insert

    ConRE
    

    Expression

    Bacterial

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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27. MSCV h c-MYC IRES GFP

    Plasmid

    #18119

    Depositor

    John Cleveland

    Insert

    c-MYC (MYC Human)
    

    Use

    Retroviral

    Tags

    GFP

    Mutation

    The CUG which is the start site for Myc I is pres…

    Available Since

    June 9, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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28. lenti-RfxCas13d-Blast-BFP

    Plasmid

    #196726

    Purpose

    Sortable and selectable RfxCas13d.

    Depositor

    Bernhard Schmierer

    Insert

    RfxCas13d-T2A-BSD-TagBFP
    

    Use

    CRISPR and Lentiviral

    Tags

    SV40 NLS

    Promoter

    EF1a

    Available Since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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29. CMV-hEAAT3

    Plasmid

    #32815

    Depositor

    Susan Amara

    Insert

    hEAAT3 (SLC1A1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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30. pFX-mCherry-EEA1

    Plasmid

    #174452

    Purpose

    Express mCherry with early endosome-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    mCherry with early endosome-targeting sequence
    

    Tags

    early endosomal antigen 1 (EEA1)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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