We narrowed to 22,307 results for: CRISPR

1. 3xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2 probasin_mTQ2_FlpO

   Plasmid

   #68405

   Purpose

   The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex8 and SMAD4 ex2. are expressed by the U6 promoter

   Depositor

   Jannik Elverløv-Jakobsen

   Inserts

   3xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2
   FlpO
   

   Use

   CRISPR

   Tags

   mTurquoise2 (BFP)

   Expression

   Mammalian

   Mutation

   Promoter

   Synthetic Probasin ARRx2 and U6

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRnc-gGFP161

   Plasmid

   #189536

   Purpose

   Expresses E. Coli RNase III under control of pBAD promoter and constitutively expresses gUTR-161-GFP

   Depositor

   Marc Ostermeier

   Article

   Hauk et al CRISPR J. 2022 Dec 9. doi: 10.1089/crisp

   Inserts

   RNase III
   gUTR-161-GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N/A

   Promoter

   J23119 and pBAD

   Available since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMB63

   Plasmid

   #47945

   Purpose

   Expresses C. elegans optimized Cas9 from the eef-1A.1 (eft-3) promoter

   Depositor

   Mike Boxem

   Article

   Waaijers et al Genetics. 2013 Aug 26.

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, and egl-13 NLS

   Expression

   Worm

   Mutation

   Promoter

   eef-1A.1 (eft-3)

   Available since

   Dec. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. NAX_CAGGS_CAS9_m4_VPR_Puro

   Plasmid

   #167887

   Purpose

   PiggyBac compatible plasmid expressing dCas9-VPR

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-VPR
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCfB5191

   Plasmid

   #126911

   Purpose

   Plasmid containing gRNA expression cassettes targeting the X-4 and XII-5 integration sites in Saccharomyces cerevisiae

   Depositor

   Irina Borodina

   Article

   Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

   Insert

   X-4 and XII-5 gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMOD8A-RGR-r10

   Plasmid

   #74370

   Purpose

   This plasmid constitutively expresses gRNA-r10 from an AHD1 promoter using the RGR design. Integrates in the W303 HIS locus and restores HIS function.

   Depositor

   Eric Klavins

   Article

   Gander et al bioRxiv 041871

   Insert

   RGR-r10
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   April 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. sgRNA AAVS1

   Plasmid

   #128119

   Purpose

   Co-expresses sgRNA AAVS1, SpyCas9 scaffold (F+E) and GFP (transfection marker)

   Depositor

   Dominik Niopek

   Article

   Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

   Insert

   sgRNA AAVS1 + GFP
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   RSV/U6

   Available since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLH-stsgRNA1.1

   Plasmid

   #64116

   Purpose

   Vector for expression of St1 sgRNA1.1 in mammalian cells

   Depositor

   Thoru Pederson

   Article

   Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

   Insert

   St1 sgRNA1.1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTX172

   Plasmid

   #89259

   Purpose

   Express STU (Single Transcriptional Unit) Cas9 with Maize Ubiquitin1 promoter in plants

   Depositor

   Daniel Voytas

   Article

   Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. NAX_CAGGS_CAS9_m4_VPR_EYFP

    Plasmid

    #167882

    Purpose

    PiggyBac compatible plasmid expressing dCas9-VPR

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTX176

    Plasmid

    #89260

    Purpose

    Express estrodial-inducible STU (Single Transcriptional Unit) Cas9 in plants

    Depositor

    Daniel Voytas

    Article

    Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEF:iCas9

    Plasmid

    #138267

    Purpose

    Expression of mTN3-GGS6-dCas9 (iCas9) in human.

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

    Insert

    mTN3-GGS6-dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Cas9-D10A, H840A

    Promoter

    Ef1a

    Available since

    March 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMOD8A-iRGR-r11

    Plasmid

    #74369

    Purpose

    This plasmid constitutively expresses gRNA-r11 from an AHD1 promoter using the insulated RGR design. Integrates in the W303 HIS locus and restores HIS function.

    Depositor

    Eric Klavins

    Article

    Gander et al bioRxiv 041871

    Insert

    RGR-r11
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    AHD1

    Available since

    April 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pColE1_sgRNA_M7NS_AmpR

    Plasmid

    #119157

    Purpose

    Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with seven mismatches in the non-seed region

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with seven mismatches in the non-seed region
    

    Use

    Crispr

    Tags

    Expression

    Mutation

    Promoter

    J23119

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pColE1_sgRNA_M2S_AmpR

    Plasmid

    #119154

    Purpose

    Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with two mismatches in the seed region

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with two mismatches in the seed region
    

    Use

    Crispr

    Tags

    Expression

    Mutation

    Promoter

    J23119

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pZmU3-gRNA

    Plasmid

    #53061

    Purpose

    Transcription of guide RNA (gRNA) in maize cells

    Depositor

    Caixia Gao

    Article

    Liang et al J Genet Genomics. 2014 Feb 20;41(2):63-8

    Insert

    zmU3 promoter - gRNA
    

    Use

    CRISPR; Plant expression

    Tags

    Expression

    Mutation

    Promoter

    maize U3

    Available since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSIBR005

    Plasmid

    #177668

    Purpose

    Constitutive expression of FnCas12a

    Depositor

    John van der Oost

    Article

    Patinios et al Nucleic Acids Res. 2021 Oct 6. pii: 6382

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacUV5

    Available since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTX168

    Plasmid

    #89257

    Purpose

    Express STU (Single Transcriptional Unit) Cas9 with CaMV 35s promoter in plants

    Depositor

    Daniel Voytas

    Article

    Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEM-WingGFP-tan

    Plasmid

    #83473

    Purpose

    EGFP selectable marker for use in two-stage CRISPR-mediated allele replacement strategies in Drosophila. D. americana tan homology arms can be replaced with locus of choice.

    Depositor

    Patricia Wittkopp

    Article

    Lamb et al Fly (Austin). 2016 Aug 5:1-12.

    Insert

    WingGFP-tan
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    Hsp70 and enhancer from D. melanogaster yellow ge…

    Available since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBBK93 pHomL-TDH3p-Neon(gfp)-SSA1t-HomR (AmpR)

    Plasmid

    #179077

    Purpose

    Vector containing a yeast integration cassette for expressing TDH3p-Neon(gfp), an overexpressed (bright) marker for the cytosol

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    TDH3p-Neon(gfp)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. pX330A_FokI-1x4

    Plasmid

    #63604

    Purpose

    Expresses FokI-dCas9 and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

    Insert

    FokI-dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. ptreCas9-mKate2ps-T1gRNA

    Plasmid

    #62715

    Purpose

    Inducible; gRNA seq is ATGAGAATCAAGGCGGTCGA

    Depositor

    Leonidas Bleris

    Article

    Moore et al Nucleic Acids Res. 2015 Jan 30;43(2):129

    Insert

    Cas9–mKate2ps
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. NAX_CAGGS_CAS9_m4_EYFP

    Plasmid

    #167866

    Purpose

    PiggyBac compatible plasmid expressing dCas9

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCF824_U6-Sau-sgRNA-EF1a-mCherry2_lenti

    Plasmid

    #138318

    Purpose

    Lenti expression of mCherry2 with U6 promoter for SauCas9 sgRNA targeting GFP

    Depositor

    Jennifer Doudna

    Article

    Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

    Insert

    SauCas9 GFP sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. NAX_CAGGS_CAS9_m4_KRAB_EBFP2

    Plasmid

    #167873

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-KRAB
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pX330A_dCas9-1x6

    Plasmid

    #63600

    Purpose

    Expresses dCas9 and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

    Insert

    humanized S. pyogenes dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D1361Y

    Promoter

    CBh

    Available since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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27. pYPQ159

    Plasmid

    #69308

    Purpose

    Gateway entry vector with hSpCas9D10A

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Insert

    hSpCas9D10A (Human codon optimized)
    

    Use

    CRISPR

    Tags

    3XFLAG

    Expression

    Mutation

    D10A mutation; NLS fusion

    Promoter

    Available since

    Nov. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pX330A_FokI-1x7

    Plasmid

    #63607

    Purpose

    Expresses FokI-dCas9 and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

    Insert

    FokI-dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pRU205

    Plasmid

    #167685

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    PcUBQ4-2::asCpf1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pRU321

    Plasmid

    #167681

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    PcUB4-2::asCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge