We narrowed to 13,482 results for: cas9 genes

1. pMOD_A-AtUBI10-dCas9-24XGP41

   Plasmid

   #202011

   Purpose

   Moclo level 1 - Module A, Promoter: AtUBI10, Gene: dCas9-24XGP41, Terminator: HSP

   Depositor

   Michael Smanski

   Article

   Casas-Mollano et al Nucleic Acids Research, Volume 51, Issue

   Insert

   dCas9-24XGP41
   

   Use

   Synthetic Biology; Moclo level 1 vector

   Tags

   Expression

   Mutation

   Promoter

   AtUBI10

   Available since

   July 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pB-CAGGS-dCas9-KRAB-MeCP2

   Plasmid

   #110824

   Purpose

   PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2

   Depositor

   George Church, Alejandro Chavez

   Article

   Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

   Insert

   dCas9-KRAB-MeCP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

   Promoter

   CAGGS

   Available since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPBT/EFS-Cas9-PuroTk

   Plasmid

   #170542

   Purpose

   Expresses spCas9 with a P2A junction to the puromycin resistance gene directly fused to the thymidine kinase

   Depositor

   Jacob Giehm Mikkelsen

   Article

   Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

   Insert

   PuroTK
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. FASTHDR-tRNApromo-sgRNA-espCas91_1

   Plasmid

   #167210

   Purpose

   Plasmid for easy cloning of a sgRNA sequence. The Plasmid contain a tRNA promoter to facilitate the expression of any sgRNA sequence and also expresses eSpCas9(1.1)

   Depositor

   Oscar Perez

   Article

   Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA-dCas9-p300 Core

   Plasmid

   #61357

   Purpose

   encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

   Insert

   S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, S. pyogenes, Synthetic)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Flag, HA, and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   CMV

   Available since

   April 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMSCV-Cas9-2A-GFP-sgRNA

   Plasmid

   #124889

   Purpose

   Contains spCas9-2A-GFP and guide RNA scaffold in single retroviral vector

   Depositor

   Harvey Lodish

   Article

   Li et al Haematologica. 2016 Jun;101(6):e216-9. d

   Insert

   Cas9
   

   Use

   Retroviral

   Tags

   2A-GFP

   Expression

   Mutation

   Promoter

   MSCV

   Available since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-TRE-dCas9-KRAB-MeCP2

   Plasmid

   #122267

   Purpose

   SP-dCas9-KRAB-MeCP2 with doxycycline-inducible expression

   Depositor

   Andrea Califano

   Article

   Tan et al bioRxiv 2021.06.28.449297

   Insert

   dCas9-KRAB-MeCP2
   

   Use

   Tags

   dCas9-KRAB-MeCP2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. px551-mSncg-0.27kb-HA-SpCas9

   Plasmid

   #153208

   Purpose

   Truncated Mouse gamma-synuclein (mSncg-0.27kb) promoter-mediates HA and Cas9 expression in retinal ganglion cells

   Depositor

   Yang Hu

   Article

   Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

   Insert

   Cas9
   

   Use

   AAV and CRISPR

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Truncated mSncg (0.27kb)

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. dCas9-5xGCN4-P2A-BFP

   Plasmid

   #184438

   Purpose

   dCas9 fused with 5 copies of GCN4 epitope

   Depositor

   Rhys Allan

   Article

   Chan et al Nat Commun. 2022 Sep 23;13(1):5582. doi:

   Insert

   dCas9-5xGCN4
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pME-Cas9-T2A-GFP

    Plasmid

    #63155

    Purpose

    Middle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS and followed by inframe GFP. Cas9 and GFP are separated by the sequence of a T2A self-cleaving peptide.

    Depositor

    Leonard Zon

    Article

    Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

    Insert

    Cas9-T2A-GFP
    

    Use

    CRISPR; Tol2 middle entry vector for gateway

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. p-dCas9-SSAP-MS2-BB_BbsI

    Plasmid

    #183826

    Purpose

    pU6-MS2-gRNA-backbone(BbsI)-CBH-dSpCas9-T2A-EBFP

    Depositor

    Le Cong

    Article

    Wang et al Nat Cell Biol. 2022 Feb;24(2):268-278. d

    Insert

    U6-guideRNA-dCas9-T2A-EBFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBH

    Available since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAC154-dual-dCas9VP160-sgExpression

    Plasmid

    #48240

    Purpose

    Dual expression construct expressing both dCas9VP160 and sgRNA from separate promoters

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    HA-Tag, HA-tag, and VP160

    Expression

    Mammalian

    Mutation

    D10A H840A

    Promoter

    Available since

    Sept. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lenti-Dual-iCas9-CMV

    Plasmid

    #167500

    Purpose

    Expression of dual inducible Cas9 with CMV promoter

    Depositor

    Jake Liang

    Article

    Park et al Mol Ther Nucleic Acids. 2022 Apr 27;28:6

    Insert

    Cas9
    

    Use

    CRISPR, Lentiviral, and Luciferase

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG dCas9-KRAB-2A-EGFP

    Plasmid

    #92396

    Purpose

    CAG-driven ubiquitous expression of catalytically inactive Cas9 fused to KRAB repressor domain. Contains 2A-EGFP reporter. For targeted enhancer inactivation in chicken embryos.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Development. 2018 Feb 23;145(4). pii: de

    Insert

    dCas9_KRAB_2A_EGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSpCas9(BB)-2A-tomato SOX2-sgRNA

    Plasmid

    #196190

    Purpose

    Editing human SOX2 locus

    Depositor

    Jürgen Knoblich

    Article

    Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

    Insert

    SOX2 (SOX2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. Lenti-Dual-iCas9-EFS

    Plasmid

    #167501

    Purpose

    Expression of dual inducible Cas9 with EFS promoter

    Depositor

    Jake Liang

    Article

    Park et al Mol Ther Nucleic Acids. 2022 Apr 27;28:6

    Insert

    Cas9
    

    Use

    CRISPR, Lentiviral, and Luciferase

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCXLE-dCas9VP192-T2A-EGFP

    Plasmid

    #69536

    Purpose

    Episomal plasmid encoding dCas9VP192

    Depositor

    Timo Otonkoski

    Article

    Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

    Insert

    dCas9-dCas9VP192-EGFP
    

    Use

    CRISPR

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Nov. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. dCas9-NS3-NLS/VPR

    Plasmid

    #112244

    Purpose

    Encodes the drug-preservable Cas9-NS3-NLS/VPR, which can be used to activate gene expression when combined with an NS3 inhibitor and appropriately designed sgRNA.

    Depositor

    John Ngo

    Article

    Tague et al Nat Methods. 2018 Jul;15(7):519-522. doi

    Insert

    dCas9-NS3-NLS/VPR
    

    Use

    Tags

    AU1 (N term on NS3) and HA (C term on NS3)

    Expression

    Mammalian

    Mutation

    NS3 mutant T54A

    Promoter

    CMV

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUb-Cas9-EGFP_cU6:6-sgRNA

    Plasmid

    #190597

    Purpose

    The plasmid encodes S. pyogenes Cas9 and eGFP separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoter.

    Depositor

    Claudia Rueckert

    Article

    Torres et al Insects. 2022 Sep 19;13(9):856. doi: 10.

    Inserts

    Cas9
    sgRNA
    

    Use

    CRISPR

    Tags

    EGFP - separated by T2A

    Expression

    Mutation

    Promoter

    Aedes aegypti polyubiquitin and Culex quinquefasc…

    Available since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pENTR-L3-SpCas9-HF1-2A-EGFP-L2

    Plasmid

    #141048

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    SpCas9-HF1-2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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