We narrowed to 6,726 results for: tac
-
Plasmid#194494Purposeexpressing SARS-CoV-2 XBB spike protein for pseudovirus productionDepositorInsertSpike (XBB) (S SARS-CoV-2)
UseTagsExpressionMammalianMutationV83A, Y144-,H146Q, Q183E, V213E, G339H, L368I, R3…PromoterCMVAvailable sinceJan. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1 human PP2A B56α full length
Plasmid#132635Purposeexpresses human PP2A B56α full length in E. coliDepositorInsertPP2A B56α
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV-KrasG12D/sgKras/Cre
Plasmid#99851PurposeExpresses Cre-recombinase, a barcoded HDR template to introduce a G12D mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.DepositorInsertKras (Kras Mouse)
UseAAV and Cre/LoxTagsExpressionMammalianMutationAvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…PromoterAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28 His-MBP-TEV-BAP-Human BCKDK
Plasmid#232116PurposeFor bacterial expression of Human BCKDK (AA31–412, missing precursor peptide) with an N-terminal biotin acceptor peptide and a cleavable His-MBP purification tag.DepositorInsertBCKDK (BCKDK Human)
UseTags6 x His, Biotin acceptor peptide (BAP), Maltose-B…ExpressionBacterialMutationPromoterT7 PromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS426-HsFECH
Plasmid#174524PurposeExpresses human ferrochelatase (FECH) in yeastDepositorInsertFECH with yeast HEM15 promoter and terminator (FECH Synthetic, Human, Budding Yeast)
UseTagsExpressionYeastMutationLacks original mitochondrial targeting sequence (…PromoterHEM15 (yeast, 257 bp)Available sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-KrasG12V/sgKras/Cre
Plasmid#99854PurposeExpresses Cre-recombinase, a barcoded HDR template to introduce a G12V mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.DepositorInsertKras (Kras Mouse)
UseAAV and Cre/LoxTagsExpressionMammalianMutationAvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…PromoterAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-U6-sgCTG-CAG-Cas9D10A nickase-Blast
Plasmid#216733PurposeExpresses SpCas9-D10A nickase under a CAG promoter together with a blasticidin resistance gene, along with a U6 promoter driving the sgCTG (target sequence: (CUG)6). Suitable for lentivirus packaging.DepositorArticleInsertCas9-D10A nickase and sgCTG
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10APromoterU6 and CAGAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX551-miniCMV-SpCas9D10A nickase
Plasmid#216735PurposeExpresses SpCas9-D10A nickase from a miniCMV promoter. For AAV packaging. Derived from pX551-miniCMV-SpCas9, which was a gift from Alex Hewitt (Addgene #107031)DepositorArticleInsertCas9-D10A nickase
UseAAV and CRISPRTagsExpressionMammalianMutationD10APromoterT7 and mini-CMVAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-KrasG13C/sgKras/Cre
Plasmid#99856PurposeExpresses Cre-recombinase, a barcoded HDR template to introduce a G13C mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.DepositorInsertKras (Kras Mouse)
UseAAV and Cre/LoxTagsExpressionMammalianMutationAvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…PromoterAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
MADR pDonor TagBFP2-3XFlag (cyto) WPRE
Plasmid#129421PurposeMADR donor plasmid for FlpO+Cre-mediated insertion of a TagBFP2-3XFlag cytoplasmic reporter proteinDepositorInsertTagBFP2-3Flag
UseMosaic analysis for dual recombinase-mediated cas…Tags3X Flag epitope tagExpressionMutationPromoternone (3X polyA to mitigate episomal expression)Available sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXPR_BRD003 PSMB5 guide 1
Plasmid#117073Purposesingle guide RNA targeting PSMB5; guide 1DepositorInsertLMPX (PSMB5 Human)
UseCRISPRTagsExpressionMutationPromoterhU6Available sinceMarch 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MBP-g0s2
Plasmid#179294PurposeE. coli expression plasmid (Ptac promoter) for full-length human G0S2 with N-terminal mature maltose binding protein + enterokinase cleavage siteDepositorInsertG0S2 (G0S2 Human)
UseTagsmature maltose binding protein + enterokinase cle…ExpressionBacterialMutationfull-length G0S2, residues 1-103 from accession n…PromoterPtacAvailable sinceFeb. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-KrasG12A/sgKras/Cre
Plasmid#99849PurposeExpresses Cre-recombinase, a barcoded HDR template to introduce a G12A mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.DepositorInsertKras (Kras Mouse)
UseAAV and Cre/LoxTagsExpressionMammalianMutationAvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…PromoterAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV-U6-sgPlxnb2-KO-2-EF1a-LibVec
Plasmid#239598Purposeexpresses guide#2 to knock-out mouse Plxnb2, via CRISPR-Cas9DepositorInsertPlxnb2 (Plxnb2 Mouse)
UseAAVTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28 MBP-TEV-Rat BCKDK-His
Plasmid#232119PurposeFor bacterial expression of His-tagged Rat BCKDK (AA31-412, missing precursor peptide) with a cleavable MBP purification tag.DepositorInsertBCKDK (Bckdk Rat)
UseTags6 x His, Maltose-Binding Protein (MBP), and Tev S…ExpressionBacterialMutationPromoterT7 PromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLViN-iRFP670-α-cateninA+ΔβH
Plasmid#229707PurposeLentiviral expression of iRFP670-alpha-cateninA+delta-beta-H in mammalian cellsDepositorInsertCTNNA1 (alpha-catenin) Human (CTNNA1 Human)
UseLentiviralTagsiRFP670ExpressionMammalianMutationamino acids 670-673, RAIM--> GSGSPromoterCMV promoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
IL2-AHDC2-2A-CherryRhoAQ63L in pmCherryN1
Plasmid#187292PurposeExpress pmCherry-IL2-AH-deltaC2-2A-RhoA Q63LDepositorUseTagsmCherryExpressionMammalianMutationAH-deltaC2, RhoA Q63LPromoterCMVAvailable sinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLD-puro-Cc-CR-PARK7WT-VA
Plasmid#115187PurposeLentiviral transduction and expression of CRISPR/Cas9-resistant PARK7WT into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)DepositorInsertCR (CRISPR/Cas9-resistant)-PARK7WT (PARK7 Human)
UseLentiviralTagsVersatile affinity tag (2XStreptactin II-6XHis-3X…ExpressionMutationc.150G>A (silent when translated to protein, c…PromoterCMVAvailable sinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLD-puro-Cc-CR-PARK7V51G-VA
Plasmid#115188PurposeLentiviral transduction and expression of CRISPR/Cas9-resistant PARK7V51G into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)DepositorInsertCR (CRISPR/Cas9-resistant)-PARK7V51G (PARK7 Human)
UseLentiviralTagsVersatile affinity tag (2XStreptactin II-6XHis-3X…ExpressionMutationc.150G>A (silent when translated to protein, c…PromoterCMVAvailable sinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLD-puro-Cc-CR-PARK7C53A-VA
Plasmid#115189PurposeLentiviral transduction and expression of CRISPR/Cas9-resistant PARK7C53A into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)DepositorInsertCR (CRISPR/Cas9-resistant)-PARK7C53A (PARK7 Human)
UseLentiviralTagsVersatile affinity tag (2XStreptactin II-6XHis-3X…ExpressionMutationc.150G>A (silent when translated to protein, c…PromoterCMVAvailable sinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLD-puro-Cc-CR-PARK7H126A-VA
Plasmid#115190PurposeLentiviral transduction and expression of CRISPR/Cas9-resistant PARK7H126A into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)DepositorInsertCR (CRISPR/Cas9-resistant)-PARK7H126A (PARK7 Human)
UseLentiviralTagsVersatile affinity tag (2XStreptactin II-6XHis-3X…ExpressionMutationc.150G>A (silent when translated to protein, c…PromoterCMVAvailable sinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLD-puro-Cc-CR-PARK7E163K-VA
Plasmid#115191PurposeLentiviral transduction and expression of CRISPR/Cas9-resistant PARK7E163K into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)DepositorInsertCR (CRISPR/Cas9-resistant)-PARK7E163K (PARK7 Human)
UseLentiviralTagsVersatile affinity tag (2XStreptactin II-6XHis-3X…ExpressionMutationc.150G>A (silent when translated to protein, c…PromoterCMVAvailable sinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
hNTa1-qgRNA-pYJA5
Plasmid#217779PurposeNon-targeting control 1 qgRNA-pYJA5 plasmid from the T.gonfio library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloningDepositorInsertQuadruple sgRNA-expression cassette for nontargeting control for gene activation and epigenetic silencing
UseCRISPR, Lentiviral, and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman U6, mouse U6, human H1, human 7SKAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28 E2 LBD-Tev-E1a-His
Plasmid#232117PurposeFor bacterial expression of a fusion protein containing from N to C-terminus: the lipoyl-binding domain of Human E2, a TEV protease site, a peptide from Human E1a, and a His tag.DepositorInsertDBT (DBT Human)
UseTags6xHis, RIGHHSTSDDSSAY (AA331 to 345 (preprocessin…ExpressionBacterialMutationPromoterT7 PromoterAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_SARS2_omicron_BA.2.75
Plasmid#190674Purposeexpressing SARS-CoV-2 BA.2.75 spike protein for pseudovirus productionDepositorInsertSpike (S SARS-CoV-2)
UseTagsExpressionMammalianMutationK147E, W152R, F157L, I210V, G257S, D339H, G446S, …PromoterCMVAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-RRAR-OMICRON.BA.5
Plasmid#213071PurposeMammalian cell expression of SARS-CoV-2 Spike protein of Omicron.5 with with furin side intactDepositorInsertpαH-S-RRAR-OMICRON.BA.5 (S )
UseTagsHRV 3C cleavage site (before tags) C terminal on …ExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685, furin site inta…PromoterAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLCKO-CMV-SARS-CoV-2-S-Bel-P5_5-7-IRES-mNeonGreen-NES/PKI-P2A-Blasticidin
Plasmid#237480PurposeEncodes full-length SARS-CoV-2 Spike protein (Belgium/GHB-03021 isolate), featuring the E484D substitution, which likely emerged during passaging and enhances TMEM106B binding.DepositorInsertSARS-CoV-2 Spike protein (Belgium/GHB-03021 isolate)
UseLentiviralTagsIRES-mNeonGreen-NES/PKI and P2A-BlasticidinExpressionMammalianMutationS484D + S813I + deleted amino acids 68-76 + 676-6…PromoterCMVAvailable sinceJuly 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SARS2SΔC-H2gp41
Plasmid#170389PurposeGeneration of SARS-CoV-2 spike pseudotyped lentivirusDepositorInsertSARS-CoV-2 Spike (S SARS-CoV-2)
UseTagsHIV gp41ExpressionMammalianMutationDeletion of C terminal and fusion with H2 from HI…PromoterCMVAvailable sinceJune 23, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pαH-S-RRAR/XBB.1.5
Plasmid#212993PurposeMammalian cell expression of SARS-CoV-2 Spike protein of Omicron.XBB.1.5 with with furin side intactDepositorInsertSpike S-RRAR/XBB.1.5 (S )
UseTagsHRV 3C cleavage site (before tags) C terminal on …ExpressionMammalianMutationEctodomain (AAs 1-1208); furin site intake; (T19I…PromoterCMVAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
MADR pDonor TagBFP2-V5-HRAS G12VWPRE
Plasmid#129420PurposeMADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic Hras G12V with a TagBFP2 fusionDepositorInsertTagBFP2-V5-HRAS G12V (HRAS Human, Entacmaea quadricolor (TagBFP))
UseMosaic analysis for dual recombinase-mediated cas…TagsTagBFP2-V5 tagExpressionMutationG12VPromoternone (3x polyA to mitigate episomal expression)Available sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiSAMv2 C2CD4A/B-enh1-guide2
Plasmid#125466PurposeCRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs182222907 (C2CD4A/B GWAS locus)DepositorInsertdCas9-VP64-2A-BlastR
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1a core and U6Available sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-Hygro-2A-TMRPSS2
Plasmid#170390PurposeGeneration of stable lines to enhance SARS-CoV-2 infectivity in ACE2 expressing cellsDepositorInsertTMPRSS2 (TMPRSS2 Human)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceJune 23, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
MADR pDonor TagBFP2-V5-HRAS G12V WPRE RCE
Plasmid#131730PurposeMADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic Hras G12V with a TagBFP2 fusion compatible with RCE:loxP mouse strainDepositorInsertTagBFP2-V5-HRAS G12V (HRAS Human, Entacmaea quadricolor (TagBFP))
UseCre/Lox and Synthetic Biology; Mosaic analysis fo…TagsTagBFP2-V5 tagExpressionMammalianMutationG12VPromoternone (4x polyA to mitigate episomal expression)Available sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR C2CD4AB-enh1-guide2
Plasmid#125401PurposeCRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs182222907 (C2CD4A/B GWAS locus)DepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterhPGK and U6Available sinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-hUV6-sgRNA-dCas9-KRAB-TagBFP2 (identifier AAAA-0244)
Plasmid#202553PurposeNontargeting control vector with TagBFP2DepositorInsertHumanized dCas9-KRAB-TagBFP2 (tag blue fluorescent protein 2) (TRIM28 S. Pyogenes (dCas9), H. sapiens (KRAB), Entacmaea quadricolor (TagBFP2))
UseCRISPR and LentiviralTags3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2ExpressionMutationPuromycin-resistance gene in the pLV hU6-sgRNA hU…PromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-5X-1-GST-Armadillo-delta4-6
Plasmid#242226PurposeExpression of GST-tagged human β-catenin Armadillo repeats ∆4-6 without IDRs (deletion of a.a. 2-133, 268-394, and 672-781) in E. coliDepositorInsertCTNNB1 (CTNNB1 Human)
UseTagsGSTExpressionBacterialMutationDeleted N terminal IDR, arm 4-6, and C terminal I…PromotertacAvailable sinceAug. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
Split 'n coexpress CUL3-RBX1
Plasmid#52293Purposebacterial co-expression of N-terminal Cul3, C-terminal Cul3, and Rbx1DepositorUseTagsGST, IRES, and TEVExpressionBacterialMutationcontains aa 1-309, contains aa 310-768, and conta…PromoterIRES and tacAvailable sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
-
-
-
pCGLH-luciferase
Plasmid#194979PurposepCGLH control plasmidDepositorInsertluciferase
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
-
SGEP-shRLuc-308
Plasmid#188667Purposecontrol shRNADepositorInsertshRLuc
UseCRISPR and LentiviralTagsExpressionMutationWTPromoterAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
SGEP-shRLuc-802
Plasmid#188668Purposecontrol shRNADepositorInsertshRLuc
UseCRISPR and LentiviralTagsExpressionMutationWTPromoterAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
ipUSEPR-sg-Hs-PCNA
Plasmid#188686Purposecontrol sgRNADepositorInsertsgPCNA (PCNA Human)
UseCRISPR and LentiviralTagsExpressionMutationWTPromoterAvailable sinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV CAG Barcode6
Plasmid#229068PurposeExpression mappingDepositorInsertCAG Barcode6
UseAAVTagsExpressionMutationPromoterAvailable sinceDec. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV syn Barcode1
Plasmid#229062PurposeExpression mappingDepositorInsertSyn Barcode1
UseAAVTagsExpressionMutationPromoterAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV CAG Barcode5
Plasmid#229067PurposeExpression mappingDepositorInsertCAG Barcode5
UseAAVTagsExpressionMutationPromoterAvailable sinceDec. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV CAG Barcode3
Plasmid#229064PurposeExpression mappingDepositorInsertCAG Barcode3
UseAAVTagsExpressionMutationPromoterAvailable sinceDec. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO-sh-BNIP3-FL
Plasmid#100771PurposeLentiviral expression of shRNA targeting BNIP3DepositorInsertLenti-sh-BNIP3-FL
UseLentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceSept. 8, 2017AvailabilityAcademic Institutions and Nonprofits only