20,112 results

Showing: 2341 - 2370 of 20112 results

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G5-CD40

Plasmid

#121166

Purpose

pMP71 vector expressing GFP - P2A - G5 - myc - CD40

Depositor

Gabriel Victora

Article

Pasqual et al Nature. 2018 Jan 25;553(7689):496-500. d

Insert

G5-CD40

Use

Retroviral

Tags

GFP and myc

Expression

Mammalian

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1 hygro

Plasmid

#24150

Purpose

3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses hygromycin for selection.

Depositor

Bob Weinberg

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6 for shRNA

Available Since

March 15, 2010

Availability

Academic Institutions and Nonprofits only

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pMK411 (OsTIR1(F74G) mAID-EGFP-Nluc)

Plasmid

#140659

Purpose

OsTIR1(F74G)-P2A-mAID-EGFP-Nluc

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

OsTIR1(F74G) mAID-EGFP-Nluc TOL2

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2-Gal4ff.zf1

Plasmid

#61392

Purpose

mRNA synthesis of Gal4ff codon modified for zebrafish

Depositor

Harold Burgess

Article

Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

Insert

Gal4ff.zf1

Use

Zebrafish expression

Promoter

sp6

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pFX-mCherry-EEA1

Plasmid

#174452

Purpose

Express mCherry with early endosome-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

mCherry with early endosome-targeting sequence

Tags

early endosomal antigen 1 (EEA1)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV-U6sgRNA(BbsI)-PGKpuro2ABFP

Plasmid

#102796

Purpose

Retrovirus for delivery of one sgRNA (empty, bbsI)

Depositor

Sarah Teichmann

Article

Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

Insert

U6 sgRNA

Use

CRISPR and Retroviral

Expression

Mammalian

Promoter

U6

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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HA-GFP1-10-pDEST-N

Plasmid

#118368

Purpose

Bimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible N-terminal vector with BiFC-tag (HA-GFP1-10) to screen interaction partners.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

HA-GFP1-10

Expression

Mammalian

Available Since

May 1, 2019

Availability

Academic Institutions and Nonprofits only

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pLL7.0: Venus-iLID-CAAX (from KRas4B)

Plasmid

#60411

Purpose

Mammalian Expression of Venus-iLID-CAAX

Depositor

Brian Kuhlman

Article

Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

Insert

Venus-iLID-CAAX

Use

Cre/Lox and Lentiviral

Tags

Venus

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pHel3

Plasmid

#102961

Purpose

E.coli/H.Pylori shuttle vector

Depositor

Rainer Haas

Article

Heuermann et al Mol Gen Genet. 1998 Mar;257(5):519-28.

Type

Empty backbone

Use

Shuttle vector

Available Since

Nov. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pHR_pGK_LaG17_synNotch_Gal4VP64

Plasmid

#79127

Purpose

Lentiviral vector for constitutive expression of the LaG17 GFP nanobody synNotch Gal4DBDVP64 Receptor (PGK promoter)

Depositor

Wendell Lim

Article

Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

Insert

LaG17_synNotch_Gal4DBDVP64

Use

Lentiviral

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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gag-P3-pol

Plasmid

#211374

Purpose

Packaging plasmid for v3b PE-eVLP production

Depositor

David Liu

Article

An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

Insert

MMLVgag-P3-pol

Expression

Mammalian

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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HA Depdc5 pRK5

Plasmid

#46327

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

Depdc5 (DEPDC5 Human)

Tags

HA

Expression

Mammalian

Mutation

Differs from Depdc5 isoform 1 by 10 amino acids …

Available Since

July 16, 2013

Availability

Academic Institutions and Nonprofits only
EF1a- gfp-2a-puro

Plasmid

#129443

Purpose

expression of GFP and Puro R

Depositor

Oskar Laur

Insert

gfp-puro

Use

Lentiviral

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pHM6-Q74

Plasmid

#40264

Depositor

David Rubinsztein

Article

Narain et al J Med Genet. 1999 Oct;36(10):739-46.

Insert

HTT partial exon 1 Q74 (HTT Human)

Tags

6x His and HA

Expression

Mammalian

Mutation

expanded poly-Q

Promoter

CMV

Available Since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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pET-Sfp

Plasmid

#159617

Purpose

Expression plasmid for Sfp phosphopantetheinyl transferase

Depositor

Christopher T Walsh, Jun Yin

Article

Zhou et al ACS Chem Biol. 2007 May 22;2(5):337-46.

Insert

Sfp

Tags

6HIS

Expression

Bacterial

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 SARS-CoV-2 N

Plasmid

#158079

Purpose

Expresses SARS-CoV-2 nucleocapsid protein in mammalian cells

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

SARS-CoV-2 nucleocapsid (N )

Expression

Mammalian

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFPN1-human Dynamin 1aa K44A

Plasmid

#22197

Depositor

Pietro De Camilli

Article

Lee et al Proc Natl Acad Sci U S A. 2002 Jan 8. 99

Insert

human Dynamin 1aa K44A (DNM1 Human)

Tags

GFP

Expression

Bacterial and Mammalian

Mutation

K44A

Available Since

Nov. 2, 2009

Availability

Academic Institutions and Nonprofits only

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Geminin-mStrawberry reporter

Plasmid

#158690

Purpose

This plasmid is a Geminin cell cycle reporter. It has CMV promoter driving the expression of Geminin fused to mStrawberry-pGK-BSD

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

mStrawberry

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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hUbc13

Plasmid

#51131

Purpose

human ubc13, E2 for synthesis k63 linked ubiquitin chain

Depositor

Cynthia Wolberger

Article

Wiener et al Nature. 2012 Feb 22;483(7391):618-22. do

Insert

ubc13 (UBE2N Human)

Tags

His6 and SUMO

Expression

Bacterial

Promoter

T7

Available Since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-FRB-LgBiT

Plasmid

#182650

Purpose

FRB-LgBiT

Depositor

Masaharu Somiya

Article

Somiya et al Mol Pharm. 2022 May 20. doi: 10.1021/acs

Insert

FRB-LgBiT fusion

Tags

LgBiT

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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Flag-GFP11-pDEST-N

Plasmid

#118366

Purpose

Bimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible N-terminal vector with BiFC-tag (GFP11-Flag) to screen interaction partners.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

GFP11-Flag

Expression

Mammalian

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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tdTomato-C1

Plasmid

#54653

Purpose

Localization: C1 Cloning Vector, Excitation: 554, Emission: 581

Depositor

Michael Davidson

Type

Empty backbone

Tags

tdTomato

Expression

Mammalian

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

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pCRISPR

Plasmid

#42875

Purpose

A crRNA expression plasmid for targeting a specific sequence.

Depositor

Luciano Marraffini

Article

Jiang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

Insert

CRISPR-BsaI

Use

CRISPR; E.coli

Expression

Bacterial

Available Since

March 5, 2013

Availability

Academic Institutions and Nonprofits only

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pLIX403_UBAP2L_mCherry

Plasmid

#105286

Purpose

puro-selectable lentivirus vector for inducible expression of a UBAP2L-mCherry fusion protein

Depositor

Eugene Yeo

Article

Markmiller et al Cell. 2018 Jan 25;172(3):590-604.e13. do

Insert

UBAP2L (UBAP2L Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

TRE promoter, Tet ON

Available Since

Sept. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pVITRO1-Trastuzumab-IgG4/κ

Plasmid

#61887

Purpose

Expresses HER2/neu receptor specific humanized IgG4/κ antibody isotype (Trastuzumab-IgG4/κ)

Depositor

Andrew Beavil

Article

Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

Inserts

HER2/neu receptor specific humanized Gamma 4 heavy chain expression cassette
HER2/neu receptor specific humanized kappa light chain expression cassette

Expression

Mammalian

Promoter

mEF1 Prom and rEF1 Prom

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-ZMYND8

Plasmid

#65401

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

ZMYND8 (ZMYND8 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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HDAC3 Flag

Plasmid

#13819

Purpose

Mammalian expression of human histone deacetylase 3 with flag tag

Depositor

Eric Verdin

Article

Emiliani et al Proc Natl Acad Sci U S A. 1998 Mar 17. 9

Insert

HDAC3 (HDAC3 Human)

Tags

flag

Expression

Mammalian

Available Since

Feb. 19, 2007

Availability

Academic Institutions and Nonprofits only

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pBE14

Plasmid

#174653

Purpose

In vivo Cre-lox circularization in E. coli

Depositor

Huimin Zhao

Article

Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

Insert

Cre

Use

Synthetic Biology

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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rAAV2/MG1.2

Plasmid

#184541

Purpose

AAV packaging plasmid, expressing Rep2 and AAV-MG1.2 capsid

Depositor

Minmin Luo

Article

Lin et al Nat Methods. 2022 Jul 25. pii: 10.1038/s

Insert

AAV-MG1.2 capsid

Use

AAV; Helper vector for packaging

Mutation

changes on AAV9 capsid: K449R, Q588T; insertion o…

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pKM468-EGFP

Plasmid

#108434

Purpose

ORBIT integrating plasmid for C-terminal tagging with EGFP.

Depositor

Kenan Murphy

Article

Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

Insert

attB-eGFP-4xGly-TEV-Flag-6xHis

Use

Orbit integrating plasmid

Available Since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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20,112 results