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We narrowed to 9,360 results for: CAG

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Showing: 3901 - 3950 of 9360 results

  1. AA324

    Plasmid
    #215953
    Purpose
    Fragmid fragment: (guide cassette) guide expression; reverse orientation; positive control cell surface
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    rev{U6_v1; DR_v0[EnAs]; sgCD46_v4[EnAs]; DR_v1[EnAs]; sgCD47_v2[EnAs]; DR_v2[EnAs]; sgCD55_v4[EnAs];DR_v3[EnAs];sgCD81_v4[EnAs]}
    Use
    CRISPR; Fragment
    Available Since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    215953-plasmid-map-sequence-id-431481

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  2. pLCHKO_TK1_HPRT1_Lb_3

    Plasmid
    #155061
    Purpose
    Lentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and two intergenic sites & HPRT1 using LbCas12a (CHyMErA system)
    Depositor
    Benjamin Blencowe, Jason Moffat
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA targeting TK1 using SpCas9 and two intergenic sites & HPRT1 using LbCas12a
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Aug. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    155061-plasmid-map-sequence-id-308580

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  3. pLCHKOv3-mClover-intergenic_4-As

    Plasmid
    #218816
    Purpose
    Lentiviral vector expressing mClover3 along with U6 driven hybrid guide (hgRNA) targeting two intergenic sites in the human genome using SpCas9 and AsCas12a nucleases (CHyMErA system), respectively
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    intergenic hgRNA with SpCas9 tracrRNAv1 & AsCas12a DR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    218816-plasmid-map-sequence-id-436109

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  11. WT Smarca4-sfGFP

    Plasmid
    #107056
    Purpose
    Expression of wild-type Smarca4 (Brg1) with C-terminal super-folder GFP fusion.
    Depositor
    Jerry Crabtree, Courtney Hodges
    Article
    Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7
    Insert
    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
    Use
    Lentiviral
    Tags
    Super-folder GFP (sfGFP)
    Expression
    Mammalian
    Mutation
    wild-type
    Promoter
    CAG
    Available Since
    April 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    107056-plasmid-map-sequence-id-321500

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  12. pSH-Csy4-T2A-SpRFN

    Plasmid
    #85754
    Purpose
    Expresses S. pyogenes FokI-dCas9-NLS with a 25 amino acid linker (GGGGS)5 fusion. Also expresses Csy4 for cleavage of multiplexed gRNA transcripts. Derived from pSQT1601 (Addgene #53369).
    Depositor
    Lawrence Stanton
    Article
    Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:
    Insert
    Csy4-T2A-FokI-dCas9
    Use
    CRISPR
    Tags
    Csy4 and FokI fusion via 25 amino acid (GGGGS)5 l…
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CAG
    Available Since
    Jan. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    85754-plasmid-map-sequence-id-170272

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  13. WT Smarca4-V5 IRES-puro

    Plasmid
    #107058
    Purpose
    Expression of wild-type Smarca4 (Brg1)-V5 fusion with IRES puromycin resistance.
    Depositor
    Jerry Crabtree, Courtney Hodges
    Article
    Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7
    Insert
    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
    Use
    Lentiviral
    Tags
    IRES-puroR and V5
    Expression
    Mammalian
    Mutation
    wild-type
    Promoter
    CAG
    Available Since
    April 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    107058-plasmid-map-sequence-id-201960

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  14. K785R Smarca4-sfGFP

    Plasmid
    #107057
    Purpose
    Expression of dominant-negative K785R Smarca4 (Brg1) with C-terminal super-folder GFP fusion.
    Depositor
    Jerry Crabtree, Courtney Hodges
    Article
    Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7
    Insert
    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
    Use
    Lentiviral
    Tags
    Super-folder GFP (sfGFP)
    Expression
    Mammalian
    Mutation
    K785R
    Promoter
    CAG
    Available Since
    April 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    107057-plasmid-map-sequence-id-201959

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  17. pCAT077

    Plasmid
    #180511
    Purpose
    Plasmid expressing mammalian codon optimized wt PlmCasX, sgRNAv2 scaffold, and restriction sites to clone in new spacers
    Depositor
    Jennifer Doudna
    Article
    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6
    Inserts
    CasX sgRNAv2
    PlmCasX
    Use
    CRISPR
    Tags
    2x FLAG and SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG and U6
    Available Since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180511-plasmid-map-sequence-id-359731

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  20. PA-Dre

    Plasmid
    #169706
    Purpose
    Photoactivatable Dre recombinase for AAV production and expression in mammalian cells
    Depositor
    Dali Li
    Article
    Li et al Proc Natl Acad Sci U S A. 2020 Dec 29;11
    Insert
    Dre246-nMag-T2A-pMag-Dre247
    Use
    AAV
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CAG
    Available Since
    May 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    169706-plasmid-map-sequence-id-333284

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  21. pCAT100

    Plasmid
    #180512
    Purpose
    Plasmid expressing mammalian codon optimized engineered chimeric PlmCasX-R1, sgRNAv2 scaffold, and restriction sites to clone in new spacers
    Depositor
    Jennifer Doudna
    Article
    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6
    Inserts
    CasX sgRNAv2
    PlmCasX with DpbCasX R1 loop
    Use
    CRISPR
    Tags
    2x FLAG and SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG and U6
    Available Since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180512-plasmid-map-sequence-id-359730

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  24. K785R Smarca4-V5 IRES-puro

    Plasmid
    #107059
    Purpose
    Expression of dominant-negative K785R Smarca4 (Brg1)-V5 fusion with IRES puromycin resistance.
    Depositor
    Jerry Crabtree, Courtney Hodges
    Article
    Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7
    Insert
    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
    Use
    Lentiviral
    Tags
    IRES-puroR and V5
    Expression
    Mammalian
    Mutation
    K785R
    Promoter
    CAG
    Available Since
    April 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    107059-plasmid-map-sequence-id-201961

    Enlarge

  25. pCAT527

    Plasmid
    #180514
    Purpose
    Plasmid expressing mammalian codon optimized engineered chimeric PlmCasX-R1, mNeonGreen, sgRNAv2 scaffold, and restriction sites to clone in new spacers
    Depositor
    Jennifer Doudna
    Article
    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6
    Inserts
    CasX sgRNAv2
    PlmCasX with DpbCasX R1 loop-2A-mNeonGreen
    Use
    CRISPR
    Tags
    2x FLAG and SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG and U6
    Available Since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180514-plasmid-map-sequence-id-359727

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  29. pCAT105

    Plasmid
    #180510
    Purpose
    Plasmid expressing mammalian codon optimized engineered DpbCasX-R3, sgRNAv2 scaffold, and restriction sites to clone in new spacers
    Depositor
    Jennifer Doudna
    Article
    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6
    Inserts
    CasX sgRNAv2
    DpbCasX with R3 loop
    Use
    CRISPR
    Tags
    2x FLAG and SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG and U6
    Available Since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180510-plasmid-map-sequence-id-359734

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  30. Ai27

    Plasmid
    #34630
    Purpose
    Cre-dependent expression of hChR2(H134R)-tdTomato, for optogenetic excitation
    Depositor
    Hongkui Zeng
    Article
    Madisen et al Nat Neurosci. 2012 Mar 25;15(5):793-802.
    Insert
    Floxed hChR2(H134R)-tdTomato
    Use
    Mouse Targeting
    Tags
    tdTomato
    Mutation
    His 134 has been mutated to Arg
    Promoter
    CAG
    Available Since
    Feb. 29, 2012
    Availability
    Academic Institutions and Nonprofits only
    34630-plasmid-map-sequence-id-41505

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  31. CALID

    Plasmid
    #169708
    Purpose
    Cre-activated light-inducible Dre recombinase
    Depositor
    Dali Li
    Article
    Li et al Proc Natl Acad Sci U S A. 2020 Dec 29;11
    Insert
    a double-floxed inverted Dre246-nMag-T2A-pMag-Dre247
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CAG
    Available Since
    May 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    169708-plasmid-map-sequence-id-333329

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  33. pSH-Csy4-T2A-SaRFN

    Plasmid
    #85755
    Purpose
    Expresses S.aureus FokI-dCas9-NLS with a 36 amino acid GSAT linker fusion. Also expresses Csy4 for cleavage of multiplexed gRNA transcripts. Backbone derived from pSQT1601 (Addgene #53369).
    Depositor
    Lawrence Stanton
    Article
    Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:
    Insert
    Csy4-T2A-FokI-SadCas9
    Use
    CRISPR
    Tags
    3xHA tag, Csy4, and FokI fusion via 36 amino acid…
    Expression
    Mammalian
    Mutation
    D10A, N580A
    Promoter
    CAG
    Available Since
    Jan. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    85755-plasmid-map-sequence-id-170274

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  35. PB-PE VQR

    Plasmid
    #162797
    Purpose
    All-in-one prime editor piggyBac transposon, VQR variant
    Depositor
    Tobias Cantz
    Article
    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1
    Insert
    SpCas9_H840A_VQR-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    D1135V, R1335Q, T1337R
    Promoter
    CAG
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    162797-plasmid-map-sequence-id-320036

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  36. PB-PE NG

    Plasmid
    #174554
    Purpose
    All-in-one prime editor piggyBac transposon, NG variant
    Depositor
    Tobias Cantz
    Article
    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1
    Insert
    SpCas9 NG_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    L1111R, G1218R, E1219F, A1322R, R1335V, T1337R
    Promoter
    CAG
    Available Since
    Feb. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    174554-plasmid-map-sequence-id-354966

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  37. pCAT526

    Plasmid
    #180513
    Purpose
    Plasmid expressing mammalian codon optimized wt PlmCasX, mNeonGreen, sgRNAv1 scaffold, and restriction sites to clone in new spacers
    Depositor
    Jennifer Doudna
    Article
    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6
    Inserts
    CasX sgRNAv1
    PlmCasX-2A-mNeonGreen
    Use
    CRISPR
    Tags
    2x FLAG and SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG and U6
    Available Since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180513-plasmid-map-sequence-id-359728

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  44. AAVS1-TRE3G-SOX2-3xHA-P2A-tagBFP

    Plasmid
    #163701
    Purpose
    Dox-inducible SOX2-3xHA HDR knock-in cassette into the AAVS1 locus with a tagBFP fluorescent marker linked by a self-cleaving P2A peptide.
    Depositor
    Jennifer Mitchell
    Article
    Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.
    Inserts
    SOX2-3xHA-P2A-tagBFP (SOX2 Human)
    rtTA
    T2-sgRNA
    Use
    AAV and CRISPR
    Tags
    3x-HA and P2A-tagBFP
    Expression
    Mammalian
    Promoter
    CAG and TRE3G
    Available Since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    163701-plasmid-map-sequence-id-325288

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Showing: 3901 - 3950 of 9360 results