20,033 results

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mCherry-CD81-10

Plasmid

#55012

Purpose

Localization: Tetraspanin/Membrane, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

CD81 (CD81 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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hAAVS1 1L TALEN

Plasmid

#35431

Purpose

TALEN designed to target the human AAVS1 locus

Depositor

Feng Zhang

Article

Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

Insert

hAAVS1 1L TALEN

Expression

Mammalian

Available Since

May 17, 2012

Availability

Academic Institutions and Nonprofits only

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phH1-gRNA

Plasmid

#53186

Purpose

Expresses the S. pyogenes sgRNA from the H1 promoter

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

human H1

Available Since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

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pCIneoEGFP

Plasmid

#46949

Purpose

GFP in Promega pCIneo (uses CMV promoter). Superior to pfwB for some purposes

Depositor

John Schiller

Article

Buck et al Curr Protoc Cell Biol. 2007 Dec;Chapter

Insert

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2013

Availability

Academic Institutions and Nonprofits only

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pAnc80L65AAP

Plasmid

#92307

Purpose

Adeno-associated Viral Vector (AAV) capsid Anc80L65 in AAV2Rep expression construct with endogenous AAP

Depositor

Luk Vandenberghe

Article

Landegger et al Nat Biotechnol. 2017 Mar;35(3):280-284.

Insert

Ancestral AAV Capsid Anc80L65AAP

Use

AAV and Synthetic Biology

Expression

Mammalian

Promoter

Rep2

Available Since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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HA Raptor

Plasmid

#8513

Depositor

David Sabatini

Article

Kim et al Cell 2002 Jul 26;110(2):163-75.

Insert

Raptor (RPTOR Human)

Tags

ha

Expression

Mammalian

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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hAAVS1 1R TALEN

Plasmid

#35432

Purpose

TALEN designed to target the human AAVS1 locus

Depositor

Feng Zhang

Article

Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

Insert

hAAVS1 1R TALEN

Expression

Mammalian

Available Since

April 10, 2012

Availability

Academic Institutions and Nonprofits only

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pRL443

Plasmid

#70261

Purpose

Shuttle vector suitable for mobilizing plasmids to Anabaena (Nostoc) sp. strain PCC 7120 and other cyanobacterial strains.

Depositor

Peter Wolk

Article

Elhai et al J Bacteriol. 1997 Mar;179(6):1998-2005.

Type

Empty backbone

Available Since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pDY1243-Fluorescent RADARS

Plasmid

#182540

Purpose

Express Fluorescent RADARS in bicistronic vector with mCherry as control gene

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Nat Biotechnol. 2022 Oct 27. pii: 10.103

Insert

mNeon

Expression

Mammalian

Promoter

CMV

Available Since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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ph7SK-gRNA

Plasmid

#53189

Purpose

Expresses the S. pyogenes sgRNA from the 7SK promoter

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

human 7SK

Available Since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

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pCI-EGFP-NR2b wt

Plasmid

#45447

Depositor

Andres Barria, Robert Malinow

Article

Barria et al Neuron. 2002 Jul 18;35(2):345-53.

Insert

NR2B (Grin2b Rat)

Tags

EGFP

Expression

Mammalian

Mutation

EGFP inserted after the predicted signal peptide …

Promoter

CMV

Available Since

June 7, 2013

Availability

Academic Institutions and Nonprofits only

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Sec61b-V5-TurboID

Plasmid

#166971

Purpose

Expresses ER targeted TurboID for secretome biotinylation

Depositor

Hyun-Woo Rhee

Article

Kim et al Nat Commun. 2021 Sep 1;12(1):5204. doi:

Insert

Sec61b-V5-TurboID

Tags

V5

Expression

Mammalian

Promoter

CMV promoter

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCI-EGFP-NR2a wt

Plasmid

#45445

Depositor

Andres Barria, Robert Malinow

Article

Barria et al Neuron. 2002 Jul 18;35(2):345-53.

Insert

NR2A (Grin2a Rat)

Tags

EGFP

Expression

Mammalian

Mutation

EGFP inserted after the predicted signal peptide …

Promoter

CMV

Available Since

June 7, 2013

Availability

Academic Institutions and Nonprofits only

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Halo-Sec61-C-18

Plasmid

#123285

Purpose

Localization: Endoplasmic Reticulum

Depositor

Kevin McGowan

Insert

Sec61 (SEC61B Human)

Tags

HaloTag7

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2019

Availability

Academic Institutions and Nonprofits only

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LentiGuide-Hygro

Plasmid

#139462

Purpose

Lentiviral vector with gRNA scaffold and hygromycin selectable marker

Depositor

Caroline Goujon

Insert

no sgRNA inserted; resistance gene: hygroR

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a / U6

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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SP-Cas9

Plasmid

#62731

Purpose

Expresses SP-Cas9 protein in bacterial cells

Depositor

Niels Geijsen

Article

D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

Insert

SP-CAS9

Use

CRISPR

Tags

His tag and NLS

Expression

Bacterial

Mutation

Codon optimazed for e.coli

Promoter

T7

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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spNW25

Plasmid

#173484

Purpose

Expresses spNW25 in Bl21

Depositor

Huan Bao

Article

Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

Insert

spNW25

Tags

Histag

Expression

Bacterial

Available Since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pHR-Tre3G-47xCAG-12xMS2

Plasmid

#99148

Purpose

Encodes 47xCAG repeats with 12xMS2 tag, 2nd generation lentiviral vector

Depositor

Ron Vale

Article

Jain et al Nature. 2017 Jun 8;546(7657):243-247. do

Insert

47xCAG 12xMS2 hairpins

Use

Lentiviral

Expression

Mammalian

Promoter

TRE3G

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pAS_4xMma PylT_FLAG-Mma PylRS AF

Plasmid

#140023

Purpose

Plasmid with 4xMmaPylT cassette and MmaPylRS AF for amber suppression; for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

Mma PylRS (pylS Methanosarcina mazei)

Tags

FLAG

Expression

Mammalian

Mutation

Y271A, Y349F

Promoter

EF1

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pRDA_479

Plasmid

#179099

Purpose

BE Cas expression

Depositor

John Doench, David Root

Article

Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

Insert

ABE8e (SpG)

Use

CRISPR and Lentiviral

Available Since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

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