8,818 results

1. DsRed-rab11 WT

   Plasmid

   #12679

   Depositor

   Richard Pagano

   Article

   Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

   Insert

   RAB11A (Homo sapiens) (RAB11A Human)
   

   Tags

   DsRed and HA

   Expression

   Mammalian

   Available Since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. CMV-hRad51

   Plasmid

   #125570

   Purpose

   Expresses the Rad51 protein in mammalian cells under CMV promoter

   Depositor

   David Liu

   Article

   Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

   Insert

   Rad51 (RAD51 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   May 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti PGK Hygro DEST (w530-1)

   Plasmid

   #19066

   Purpose

   3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Hygro

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBV-Luc

   Plasmid

   #16539

   Purpose

   luciferase reporter plasmid with very low basal activity

   Depositor

   Bert Vogelstein

   Article

   He et al Cell. 1999 Oct 29. 99(3):335-45.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Available Since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA4 myc PGC-1 alpha

   Plasmid

   #10974

   Purpose

   Mammalian expression of PGC-1a with myc and his tags

   Depositor

   Toren Finkel

   Article

   Ichida et al J Biol Chem. 2002 Dec 27. 277(52):50991-

   Insert

   PGC-1 alpha (PPARGC1A Human)
   

   Tags

   His and Myc

   Expression

   Mammalian

   Available Since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLminP_Luc2P_RE57

   Plasmid

   #90402

   Purpose

   Type I Interferon - ISGF3G (STAT1, STAT2, IRF9) gene reporter

   Depositor

   Ramnik Xavier

   Article

   O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Promoter

   GR

   Available Since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR-SFFV-GFP1-10

   Plasmid

   #80409

   Purpose

   Expresses GFP1-10 in mammalian cells

   Depositor

   Bo Huang

   Article

   Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

   Insert

   GFP1-10
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   Dec. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYPQ152

   Plasmid

   #69303

   Purpose

   Gateway entry vector with pco-dCas9-VP64

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Insert

   pco-dCas9-VP64 (Plant codon-optimized)
   

   Use

   CRISPR

   Mutation

   VP64 activator fusion; NLS fusion

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. MSCV_Cas9_puro

   Plasmid

   #65655

   Purpose

   Retroviral introduction of Cas9 into mammalian cell line.

   Depositor

   Christopher Vakoc

   Article

   Shi et al Nat Biotechnol. 2015 May 11. doi: 10.103

   Insert

   Cas9
   

   Use

   CRISPR and Retroviral

   Tags

   5 ' FLAG tag

   Expression

   Mammalian

   Promoter

   MSSV_LTR

   Available Since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pXAT2

    Plasmid

    #80494

    Purpose

    AAVS1 sgRNA expression vector

    Depositor

    Knut Woltjen

    Article

    Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

    Insert

    AAVS1 sgRNA-T2
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO5.sgRNA.EFS.GFP

    Plasmid

    #57822

    Purpose

    3rd generation lentiviral Vector for Sp sgRNA delivery without SpCas9, GFP, EFS Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    sgRNA
    EFS
    eGFP
    

    Use

    CRISPR and Lentiviral

    Available Since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562)

    Plasmid

    #140002

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized ABEmax(7.10) SpCas9 variant named SpG with P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…

    Promoter

    CMV and T7

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYPQ230 (LbCpf1)

    Plasmid

    #86210

    Purpose

    LbCpf1 Gateway entry plasmid

    Depositor

    Yiping Qi

    Article

    Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

    Insert

    LbCpf1
    

    Use

    CRISPR; Gateway compatible lbcpf1 entry clone

    Tags

    5' and 3' NLS

    Expression

    Plant

    Available Since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMOS008: GCaMP6F calcium sensor (cytosolic)

    Plasmid

    #163045

    Purpose

    GCaMP6F calcium sensor (cytosolic)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    GCaMP6F
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDD162 (Peft-3::Cas9 + Empty sgRNA)

    Plasmid

    #47549

    Purpose

    Cas9 + sgRNA plasmid that can be modified to cleave any Cas9 target site in the C. elegans genome.

    Depositor

    Bob Goldstein

    Article

    Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm

    Inserts

    Cas9
    Empty sgRNA
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Worm

    Mutation

    Codon optimized and with synthetic introns for C.…

    Promoter

    U6 and eef-1A.1 (eft-3)

    Available Since

    Sept. 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. p2lox-cAMPr

    Plasmid

    #99143

    Purpose

    Expresses cAMPr in mammalian cells

    Depositor

    Justin Blau

    Article

    Hackley et al Sci Signal. 2018 Mar 6;11(520). pii: 11/

    Insert

    cAMPr
    

    Use

    Cre/Lox

    Tags

    cpGFP

    Expression

    Mammalian

    Available Since

    March 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUCmini-iCAP-PHP.B

    Plasmid

    #103002

    Purpose

    non-standard AAV2 rep-AAV-PHP.B cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Deverman et al Nat Biotechnol. 2016 Feb;34(2):204-9. do

    Insert

    Synthetic construct isolate AAV-PHP.B VP1 gene
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    p41

    Available Since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. Ubc.Luc.IRES.Puro

    Plasmid

    #33307

    Depositor

    Linzhao Cheng

    Article

    Jang et al Mol Imaging. 2011 Apr;10(2):111-22.

    Insert

    Firefly Luciferase
    

    Use

    Lentiviral and Luciferase

    Expression

    Mammalian

    Promoter

    Ubc

    Available Since

    Feb. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAGGS-mCherry

    Plasmid

    #41583

    Depositor

    Phil Sharp

    Article

    Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. EJ2GFP-puro

    Plasmid

    #44025

    Depositor

    Jeremy Stark

    Article

    Bennardo et al PLoS Genet. 2008 Jun 27;4(6):e1000110. d

    Insert

    EJ2GFP egfp-based chromosomal break reporter
    

    Expression

    Mammalian

    Mutation

    insertion of I-SceI, 3 frame stop, and flanking m…

    Promoter

    pCAGGS

    Available Since

    May 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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