Skip to main content
Addgene
Showing: 351 - 400 of 78333 results

  1. TRUPATH Triple Gagust

    Plasmid
    #196054
    Purpose
    Encodes a G alpha subunit (GNAT3) with RLuc8, a G gamma subunit (GNG1) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphaGustducin-RLuc8 (GNAT3 Human)
    GGamma1-GFP2 (GNGT1 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    May 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196054-plasmid-map-sequence-id-393090

    Enlarge

  2. pTK Cas9-2A-Citrine

    Plasmid
    #92393
    Purpose
    Enhancer/reporter plasmid for tissue-specific expression of Cas9 with 2A-Citrine reporter. Contains BsmBI-flanked LacZ cloning cassette for rapid GoldenGate-based cloning of specific enhancers.
    Depositor
    Tatjana Sauka-Spengler
    Article
    Williams et al Development. 2018 Feb 23;145(4). pii: de
    Insert
    Cas9 2A Citrine
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    thymidine kinase promoter
    Available since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    92393-plasmid-map-sequence-id-190866

    Enlarge

  3. pCasper4-g-osk-TRICK

    Plasmid
    #71244
    Purpose
    oskar translation biosensor with 12x copies of modified PP7 stem loops and 6xMS2 stem loops. . full genomic region, used for P element-mediated germline transformation in Drosophila.
    Depositor
    Anne Ephrussi
    Article
    Halstead et al Science. 2015 Mar 20;347(6228):1367-671.
    Insert
    oskar (osk Fly)
    Use
    P element-mediated germline transformation
    Tags
    12xPP7 and 6xMS2
    Expression
    Insect
    Mutation
    Promoter
    osk
    Available since
    Dec. 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    71244-plasmid-map-sequence-id-127267

    Enlarge

  8. TRUPATH Triple Gaq

    Plasmid
    #196058
    Purpose
    Encodes a G alpha subunit (GNAQ) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphaQ-RLuc8 (GNAQ Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196058-plasmid-map-sequence-id-385868

    Enlarge

  20. TRUPATH Triple Gai1

    Plasmid
    #196048
    Purpose
    Encodes a G alpha subunit (GNAl1) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphai1-RLuc8 (GNAI1 Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196048-plasmid-map-sequence-id-385883

    Enlarge

  21. pLVX-EGFP-C1-centrin-1

    Plasmid
    #73331
    Purpose
    Lentiviral vector encoding centrin-1 with an N-terminal EGFP tag
    Depositor
    Manuel Thery
    Insert
    centrin-1 (CETN1 Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    73331-plasmid-map-sequence-id-139044

    Enlarge

  22. TRUPATH Triple GaoA

    Plasmid
    #196051
    Purpose
    Encodes a G alpha subunit (GNAO1 Isoform Alpha 1) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphaoA-RLuc8
    GGamma8-GFP2 (GNG8 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196051-plasmid-map-sequence-id-385865

    Enlarge

  24. pUT_mNF-Cas13d-Triplex

    Plasmid
    #227709
    Purpose
    LPUtopia matching RMCE donor plasmid with mCherry-P2A-RfxCas13d Negative-Feedback circuit. Include a 3' UTR Triplex motif as a template backbone for MONARCH 2.0 system.
    Depositor
    Gabor Balazsi
    Article
    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-
    Insert
    mCherry-P2A-RfxCas13d-Triplex
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV-d2i
    Available since
    Nov. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    227709-plasmid-map-sequence-id-450930

    Enlarge

  27. TRUPATH Triple GaZ

    Plasmid
    #196053
    Purpose
    Encodes a G alpha subunit (GNAZ) with RLuc8, a G gamma subunit (GNG1) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphaz-RLuc8 (GNAZ Human)
    GGamma1-GFP2 (GNGT1 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196053-plasmid-map-sequence-id-387469

    Enlarge

  29. pTripZ RB1 WT-3XFLAG

    Plasmid
    #209070
    Purpose
    DOX-ON inducible expression vector that expresses RB1 WT with c-terminal 3X flag tag
    Depositor
    Matthew Oser
    Article
    Oser et al Cancer Discov. 2019 Feb;9(2):230-247. do
    Insert
    human RB1 WT after LR recombination with pTripZ NEO DEST to inducibly express RB1 WT with a c-terminal 3X flag tag
    Use
    Lentiviral
    Tags
    3XFLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    209070-plasmid-map-sequence-id-412296

    Enlarge

  31. TRUPATH Triple Ga12

    Plasmid
    #196061
    Purpose
    Encodes a G alpha subunit (GNA12) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlpha12-RLuc8 (GNA12 Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196061-plasmid-map-sequence-id-385874

    Enlarge

  35. TRUPATH Triple Gai3

    Plasmid
    #196050
    Purpose
    Encodes a G alpha subunit (GNAl3) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphai3-RLuc8 (GNAI3 Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196050-plasmid-map-sequence-id-385864

    Enlarge

  36. pKLBac-FluB-trimer

    Plasmid
    #229859
    Purpose
    Influenza B/Memphis/13/03 polymerase heterotrimer (self-cleaving polyprotein)
    Depositor
    Stephen Cusack
    Article
    Reich et al Nature. 2014 Dec 18;516(7531):361-6. doi
    Inserts
    PA, Influenza B (B/Memphis/13/03)
    PB1, Influenza B (B/Memphis/13/03)
    PB2, Influenza B (B/Los Angeles/1/02)
    Use
    Tags
    2x-Streptag and 8xHis-tag
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Feb. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    229859-plasmid-map-sequence-id-462522

    Enlarge

  38. TRUPATH Triple Ga15

    Plasmid
    #196060
    Purpose
    Encodes a G alpha subunit (GNA15) with RLuc8, a G gamma subunit (GNG13) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlpha15-RLuc8 (GNA15 Human)
    GGamma13-GFP2 (GNG13 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196060-plasmid-map-sequence-id-385870

    Enlarge

  42. TRUPATH Triple Gai2

    Plasmid
    #196049
    Purpose
    Encodes a G alpha subunit (GNAl2) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphai2-RLuc8 (GNAI2 Human)
    GGamma8-GFP2 (GNG8 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196049-plasmid-map-sequence-id-385863

    Enlarge

  45. TRUPATH Triple Ga13

    Plasmid
    #196062
    Purpose
    Encodes a G alpha subunit (GNA13) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlpha13-RLuc8 (GNA13 Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196062-plasmid-map-sequence-id-385881

    Enlarge

  46. pcDNA3-TRIF-CFP

    Plasmid
    #13644
    Depositor
    Doug Golenbock
    Insert
    TRIF (TICAM1 Human)
    Use
    Tags
    CFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 12, 2007
    Availability
    Academic Institutions and Nonprofits only
    13644-plasmid-map-sequence-id-7722

    Enlarge

  47. TRUPATH Triple GaoB

    Plasmid
    #196052
    Purpose
    Encodes a G alpha subunit (GNAO1 Isoform Alpha 2) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphaoB-RLuc8
    GGamma8-GFP2 (GNG8 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196052-plasmid-map-sequence-id-387470

    Enlarge

Showing: 351 - 400 of 78333 results