163,606 results

1. pX330S-2-PITCh

   Plasmid

   #63670

   Purpose

   Expresses Cas9 nuclease and the PITCh-gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

   Insert

   humanized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available Since

   Dec. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. iRFP-H2A

   Plasmid

   #158691

   Purpose

   This plasmid encodes an iRFP fused to H2A histone nuclear marker. It has CMV promoter driving the expression of iRFP fused to H2A. It has no selection markers

   Depositor

   Ravid Straussman

   Article

   Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

   Insert

   iRFP670
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Stat3-C Flag pRc/CMV

   Plasmid

   #8722

   Depositor

   Jim Darnell

   Article

   Bromberg et al Cell 1999 Aug 6;98(3):295-303.

   Insert

   Stat3-C (Stat3 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   A662C and N664C. Residues changed to Cys renders…

   Promoter

   Available Since

   Sept. 26, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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4. pZT-C13-L1

   Plasmid

   #62196

   Purpose

   Chr.13/CLYBL TALEN expression vector

   Depositor

   Jizhong Zou

   Article

   Cerbini et al PLoS One. 2015 Jan 14;10(1):e0116032. do

   Insert

   Left CLYBL TALEN
   

   Use

   TALEN

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available Since

   April 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. SPLICS PM-ER Long P2A

   Plasmid

   #164111

   Purpose

   Detect the long Plasma membrane-Endoplasmic reticulum contact

   Depositor

   Marisa Brini, Tito Calì

   Article

   Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

   Insert

   Split YFP PM-ER Long P2A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCW57.1 CMYC

   Plasmid

   #164145

   Purpose

   Doxycycline inducible expression of c-MYC

   Depositor

   Richard Possemato

   Insert

   MYC (MYC Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Doxycycline inducible promoter

   Available Since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBABE-puro SV40 LT

   Plasmid

   #13970

   Depositor

   Thomas Roberts

   Article

   Zhao et al Cancer Cell. 2003 May . 3(5):483-95.

   Insert

   SV40 LT (SV40gp6 )
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 22, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. psfaMs-Tn5

   Plasmid

   #183637

   Purpose

   Expresses anti-mouse nanobody fused with Tn5

   Depositor

   Goncalo Castelo-Branco

   Article

   Bartosovic et al Nat Biotechnol. 2023 Jun;41(6):794-805.

   Insert

   anti mouse secondary nanobody fused to Tn5
   

   Use

   Tags

   anti-mouse nanobody

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDule2-pCNF

   Plasmid

   #85495

   Purpose

   Machinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.

   Depositor

   Ryan Mehl

   Article

   Miyake-Stoner et al Biochemistry. 2009 Jun 30;48(25):5953-62

   Insert

   para-cyanophenylalanine Mj synthetase
   

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   Y32L L65V F108W Q109M D158G I159P

   Promoter

   lpp (constitutive)

   Available Since

   Feb. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP GR

    Plasmid

    #47504

    Purpose

    Expresses glucocorticoid receptor in mammalian cells

    Depositor

    Alice Wong

    Insert

    Glucocorticoid receptor (NR3C1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. Cterminal HA WDR59 pRK5

    Plasmid

    #46328

    Depositor

    David Sabatini

    Article

    Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

    Insert

    WDR59 (WDR59 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV.CBA.eGFP.2A.P301L-Tau

    Plasmid

    #140425

    Purpose

    AAV.CBA.eGFP.2A.P301L-Tau propagation reporter P301L-2N4R-hTau

    Depositor

    Bradley Hyman

    Article

    Wegmann et al Sci Adv. 2019 Jun 26;5(6):eaaw6404. doi:

    Insert

    eGFP-2A-Tau(P301L) (MAPT Human, Synthetic)
    

    Use

    AAV

    Tags

    eGFP

    Expression

    Mutation

    changed Proline 301 to Leucine

    Promoter

    CBA

    Available Since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHR_SFFV

    Plasmid

    #79121

    Purpose

    Lentiviral vector for constitutive expression of genes (SFFV promoter)

    Depositor

    Wendell Lim

    Article

    Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. p206-Switch-ON

    Plasmid

    #217885

    Purpose

    Retroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoR

    Depositor

    Ulrich Elling

    Article

    Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

    Type

    Empty backbone

    Use

    CRISPR, Cre/Lox, and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMA3790

    Plasmid

    #70110

    Purpose

    mtDNA elimination/production of rho-0 cells

    Depositor

    Mikhail Alexeyev

    Article

    Spadafora et al PLoS One. 2016 May 2;11(5):e0154684. doi

    Inserts

    EGFP
    hUNG1 Y147A
    Neo
    

    Use

    Tags

    None, human OTC mitochondrial targeting sequence,…

    Expression

    Mammalian

    Mutation

    Y147A, aa's 1-77 removed

    Promoter

    CMV, EF1a, and SV40 and native

    Available Since

    May 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. PLIN2-V5-APEX2

    Plasmid

    #170573

    Purpose

    A C-terminal APEX2 fusion to the mouse perilipin-2 protein PLIN2

    Depositor

    James Olzmann

    Article

    Bersuker et al Dev Cell. 2018 Jan 8;44(1):97-112.e7. do

    Insert

    PLIN2 (Plin2 Mouse)
    

    Use

    Lentiviral

    Tags

    V5-APEX2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-15b-EK_C122S_His5

    Plasmid

    #49048

    Purpose

    Expresses the pentahistidine-tagged catalytic domain of bovine enteropeptidase in E. coli

    Depositor

    Hans Brandstetter

    Article

    Skala et al J Biotechnol. 2013 Dec;168(4):421-5. doi

    Insert

    Enteropeptidase, peptidase domain (TMPRSS15 Bovine)
    

    Use

    Tags

    5xHis

    Expression

    Bacterial

    Mutation

    P92R, E185D, C122S

    Promoter

    T7

    Available Since

    Jan. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBS130

    Plasmid

    #26290

    Depositor

    Tom Clandinin

    Article

    Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

    Insert

    phiC31 integrase (int Streptomyces phage phiC31)
    

    Use

    Drosophila transgenesis

    Tags

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    Available Since

    Feb. 2, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. Px461-Cas9n-Trp53-sgRNA-beta

    Plasmid

    #88847

    Purpose

    CRISPR KO of Trp53

    Depositor

    Joan Massague

    Article

    Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

    Insert

    Trp53 (Trp53 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

20. Px461-Cas9n-Trp53-sgRNA-alpha

    Plasmid

    #88846

    Purpose

    CRISPR KO of Trp53

    Depositor

    Joan Massague

    Article

    Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

    Insert

    Trp53 (Trp53 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

21. pLenti PGK Blast DEST (w524-1)

    Plasmid

    #19065

    Purpose

    3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Blast 

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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22. pAAV-SYN-sDarken

    Plasmid

    #184796

    Purpose

    Serotonin Sensor sDarken under the control of a hSyn Promoter.

    Depositor

    Olivia Masseck

    Article

    Kubitschke et al Nat Commun. 2022 Dec 6;13(1):7525. doi:

    Insert

    sDarken
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available Since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Lenti:FlipGFP-T2A-mCherry

    Plasmid

    #223198

    Purpose

    Lentiviral vector encoding FlipGFP-T2A-mCherry (w/ caspase-3 cleave site in FlipGFP for restoring GFP conformation)

    Depositor

    Yeh-Hsing Lao

    Article

    Chi et al Lab Chip. 2024 Jan 30;24(3):396-407. doi

    Insert

    FlipGFP-T2A-mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Sept. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. STAT3-GFP

    Plasmid

    #110495

    Purpose

    reporter of STAT3 signaling activity

    Depositor

    Michael Lewis

    Article

    Wei et al Stem Cells. 2014 Oct;32(10):2571-82. doi

    Insert

    4 repeats of STAT3 binding site M67 at GFP promoter region
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pH-ePPE

    Plasmid

    #183097

    Purpose

    For plant prime editing in rice plants

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2022 Mar 24. doi: 10.103

    Insert

    Nls-nCas9-NC-nls-MLV-Nls
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A in SpCas9

    Promoter

    Available Since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3 HA eIF4GI (1-1599)

    Plasmid

    #45640

    Depositor

    Nahum Sonenberg

    Article

    Yanagiya et al Mol Cell Biol. 2009 Mar;29(6):1661-9. do

    Insert

    eIF4GI transcript variant 2 (EIF4G1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHAGE-3FLAG-EEA1

    Plasmid

    #176491

    Purpose

    For lentiviral transduction of 3FLAG-EEA1

    Depositor

    Wade Harper

    Article

    Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

    Insert

    EEA1 (EEA1 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEGFP-VSVG

    Plasmid

    #11912

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Presley et al Nature. 1997 Sep 4. 389(6646):81-5.

    Insert

    VSVG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    F64L, S65T, A206K relative the wild type GFP sequ…

    Promoter

    Available Since

    Sept. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pU6_HEK3_nsgRNA(PP7)

    Plasmid

    #232444

    Purpose

    nsgRNA for PE3 facilitation of a +1 T to A prime edit on the HEK3 locus, contains a PP7 in the scaffold tetraloop

    Depositor

    Gil Westmeyer

    Article

    Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

    Insert

    PP7-tagged nicking gRNA (PE3) for HEK3+1t>a edit driven by human U6 promoter
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pV1093

    Plasmid

    #111428

    Purpose

    CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at ENO1

    Depositor

    Gerald Fink

    Article

    Vyas et al Sci Adv. 2015;1(3):e1500248.

    Insert

    CaCas9/sgRNA-BsmBI stuffer
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge