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Plasmid#223458Purposeodr-2 (promB) fluorescent neural reporter driving nuclear CyOFP1 expression (refer to NeuroPAL paper for expression)DepositorInsertodr-2(promB) (odr-2 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceAug. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEY103
Plasmid#223450Purposelin11(intron 7) fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)DepositorInsertlin-11(intron 7) (lin-11 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceAug. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEY105
Plasmid#223452Purposemgl-3 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)DepositorInsertmgl-3 (mgl-3 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceAug. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEY104
Plasmid#223451Purposemgl-1 fluorescent neural reporter driving nuclear CyOFP1 expression (refer to NeuroPAL paper for expression)DepositorInsertmgl-1 (mgl-1 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceAug. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEY107
Plasmid#223454Purposensy-5 (prom3) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)DepositorInsertnsy-5(prom3)
UseTagsExpressionWormMutationPromoterAvailable sinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEY106
Plasmid#223453Purposensf-1 fluorescent neural reporter driving nuclear TagRFP-T expression (refer to NeuroPAL paper for expression)DepositorInsertnsf-1 (nsf-1 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEY117
Plasmid#223464Purposeser-2(prom1B) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)DepositorInsertser-2(prom1B) (ser-2 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEY116
Plasmid#223463Purposesax-3 fluorescent neural reporter driving nuclear CyOFP1 expression (refer to NeuroPAL paper for expression)DepositorInsertsax-3 (sax-3 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEY140
Plasmid#223487Purposetrp-4 fluorescent neural reporter driving nuclear mCyOFP1 expression (refer to NeuroPAL paper for expression)DepositorInserttrp-4 (trp-4 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEY147
Plasmid#223494Purposeunc-86 fluorescent neural reporter driving nuclear mCyOFP1 expression (refer to NeuroPAL paper for expression)DepositorInsertunc-86 (unc-86 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEY115
Plasmid#223462Purposepdfr-1 (3-2K) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)DepositorInsertpdfr-1(3-2K) (pdfr-1 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEY145
Plasmid#223492Purposeunc-42 fluorescent neural reporter driving nuclear mCyOFP1 expression (refer to NeuroPAL paper for expression)DepositorInsertunc-42 (unc-42 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEY141
Plasmid#223488Purposetrp-4(intron 1) fluorescent neural reporter driving nuclear mCyOFP1 expression (refer to NeuroPAL paper for expression)DepositorInserttrp-4(intron 1) (trp-4 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEY146
Plasmid#223493Purposeunc-46 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)DepositorInsertunc-46 (unc-46 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEY119
Plasmid#223466Purposeser-2(promCE) fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)DepositorInsertser-2(promCE) (ser-2 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEY143
Plasmid#223490Purposeunc-31 fluorescent neural reporter driving nuclear TagRFP-T expression (refer to NeuroPAL paper for expression)DepositorInsertunc-31 (unc-31 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEY112
Plasmid#223459Purposeoig-1 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)DepositorInsertoig-1 (oig-1 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEY113
Plasmid#223460Purposeosm-10 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)DepositorInsertosm-10 (osm-10 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEY133
Plasmid#223480Purposesrz-45 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)DepositorInsertsrz-45 (srz-45 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEY130
Plasmid#223477Purposesrx-113 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)DepositorInsertsrx-113 (srx-113 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEY131
Plasmid#223478Purposesrx-105 fluorescent neural reporter driving nuclear CyOFP1 expression (refer to NeuroPAL paper for expression)DepositorInsertsrx-105 (srx-105 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEY137
Plasmid#223484Purposetax-2 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)DepositorInserttax-2 (tax-2 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEY138
Plasmid#223485Purposetol-1(3k) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)DepositorInserttol-1(3k) (tol-1 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceAug. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEY144
Plasmid#223491Purposeunc-39(3k) fluorescent neural reporter driving nuclear mCyOFP1 expression (refer to NeuroPAL paper for expression)DepositorInsertunc-39 (3k) (unc-39 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceAug. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
mFzd4CRDL(42-203)-6xHis_pAcGP67a
Plasmid#216379PurposeBaculovirus transfer vector to secrete Fzd4 cysteine-rich domain and linker (residues 42-203, codon-optimized for insect cell expression, with a C-terminal His tag) in insect cellsDepositorInsertFrizzled-4 (Fzd4 Mouse)
UseBaculovirusTags6xHis and GP64 signal sequenceExpressionInsectMutationPromoterPolyhedrinAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMS110
Plasmid#215674PurposeSplit hygromycinR landing pad insertion plasmid for ChrIIIDepositorInsert5'HA + synthetic guide site3 + 3'ΔHygR:unc-54 3' UTR + lox2272 + SEC + lox2272 + 3'HA
UseCRISPR and Cre/LoxTagsExpressionWormMutation3' ∆HYGR is promoterless and encodes aa60-341PromoterAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD101
Plasmid#212925PurposeEncodes the S. cerevisiae SUC2 (Invertase) protein (Part3b') with the Aga2 signal peptide (Part3a') assembled with pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.DepositorInsertSUC2 (SUC2 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD043
Plasmid#212913PurposeEncodes the S. cerevisiae 111 amino acid SCW4 Translational Fusion Partner Sequence as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertSCW4 (SCW4 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD084
Plasmid#212921PurposeEncodes the HA-tagged S. cerevisiae Tip1p yeast surface display anchor protein as a Type 4a part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertTIP1 (TIP1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD140
Plasmid#212927PurposeEncodes the mRuby2 fluorescent protein (Part3) without any signal peptide assembled with S. cerevisiae pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.DepositorInsertmRuby2
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD080
Plasmid#212917PurposeEncodes the S. cerevisiae Aga1p yeast surface display anchor protein as a Type 3 part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertAGA1 (AGA1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD001
Plasmid#212898PurposeEncodes S.cerevisiae AGA2 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertAGA2 (AGA2 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD008
Plasmid#212905PurposeEncodes S. cerevisiae PLB1 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertPLB1 (PLB1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD006
Plasmid#212903PurposeEncodes S. cerevisiae MFalpha1 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertMFalpha1 (MF(ALPHA)1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD083
Plasmid#212920PurposeEncodes the HA-tagged S. cerevisiae Sed1p yeast surface display anchor protein as a Type 4a part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertSED1 (SED1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD082
Plasmid#212919PurposeEncodes the HA-tagged S. cerevisiae Cwp2p yeast surface display anchor protein as a Type 4a part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertCWP2 (CWP2 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD003
Plasmid#212900PurposeEncodes S.cerevisiae ECM14 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertECM14 (ECM14 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD002
Plasmid#212899PurposeEncodes S.cerevisiae CRH1 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertCRH1 (CRH1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD007
Plasmid#212904PurposeEncodes S. cerevisiae OST1 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertOST1 (OST1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD041
Plasmid#212911PurposeEncodes the S. cerevisiae YAR066W Translational Fusion Partner Sequence as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertYAR066W (YAR066W Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD023
Plasmid#212909PurposeEncodes a hybrid S. cerevisiae OST1 pre- MFalpha1 pro- signal peptide (OST1MFapp) as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertMFalpha1 (MF(ALPHA)1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD004
Plasmid#212901PurposeEncodes S.cerevisiae SUC2 (Invertase) pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertSUC2 (SUC2 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD081
Plasmid#212918PurposeEncodes the HA-tagged S. cerevisiae Aga2p yeast surface display anchor protein as a Type 4a part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertAGA2 (AGA2 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD021
Plasmid#212907PurposeEncodes an engineered S. cerevisiae MFalpha1 pre-pro- signal peptide (MFapp8) as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertMFalpha1 (MF(ALPHA)1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD024
Plasmid#212910PurposeEncodes the wildtype S. cerevisiae MFalpha1 pre-pro- signal peptide (MFappWT) as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertMFalpha1 (MF(ALPHA)1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD042
Plasmid#212912PurposeEncodes the S. cerevisiae HSP150 Translational Fusion Partner Sequence as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertHSP150 (HSP150 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD005
Plasmid#212902PurposeEncodes S. cerevisiae KSH1 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertKSH1 (KSH1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD061
Plasmid#212915PurposeEncodes the S. cerevisiae SUC2 (Invertase) protein (minus signal peptide) as a Type 3b' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertSUC2 (SUC2 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD044
Plasmid#212914PurposeEncodes the S. cerevisiae 142 amino acid SCW4 Translational Fusion Partner Sequence as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertSCW4 (SCW4 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD022
Plasmid#212908PurposeEncodes an engineered S. cerevisiae MFalpha1 pre-pro- signal peptide (MFappOPT) as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertMFalpha1 (MF(ALPHA)1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only