We narrowed to 5,563 results for: SPI
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Plasmid#219523Purposebacterial expression of cysteine-free variant of the E. coli ribonuclease HI geneDepositorInsertcysteine-free variant of the E. coli ribonuclease HI gene
UseTagsN terminal 10xHis TagExpressionBacterialMutationCysteine free variantPromoterAvailable sinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1-nsp7
Plasmid#201026PurposeExpresses SARS-CoV-2 nsp7 in mammalian.DepositorInsertnon-structural protein 7 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionMammalianMutationPromoterT7 promoterAvailable sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-Mouse-caspase-1/11a
Plasmid#183391PurposeExpression of mammalian inflammatory caspase gene in mammalian cells by retroviral transductionDepositorInsertCARD domain of mouse caspase-1 fused to catalytic domain of mouse caspase-11 (Casp1 Synthetic, Mouse)
UseRetroviralTagsMycExpressionMutationPromoterMESVAvailable sinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pMSCV-IRES-GFP-redesigned-human-Caspase-4_ANR261ENP_D315H_M318K_T352K
Plasmid#183357PurposeExpression of mammalian inflammatory caspase gene in mammalian cells by retroviral transductionDepositorInsertCaspase-4 with ICE activity (CASP4 Human)
UseRetroviralTagsMycExpressionMutationANR261ENP, D315H, M318K, T352KPromoterMESVAvailable sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-Hybrid-caspase_canine-CARDs+mouse-Caspase-11-catalytic domain
Plasmid#183396PurposeExpression of mammalian inflammatory caspase gene in mammalian cells by retroviral transductionDepositorInsertCARD domains of canine caspase-1/4 fused to catalytic domain of mouse caspase-11
UseRetroviralTagsMycExpressionMutationPromoterMESVAvailable sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-Hybrid-caspase_canine-CARDs+human-Caspase-4-catalytic domain
Plasmid#183395PurposeExpression of mammalian inflammatory caspase gene in mammalian cells by retroviral transductionDepositorInsertCARD domains of canine caspase-1/4 fused to catalytic domain of human caspase-4
UseRetroviralTagsMycExpressionMutationPromoterMESVAvailable sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-Mouse-caspase-1/11b
Plasmid#183392PurposeExpression of mammalian inflammatory caspase gene in mammalian cells by retroviral transductionDepositorInsertCARD domain of mouse caspase-1 fused to mouse caspase-11 (Casp1 Synthetic, Mouse)
UseRetroviralTagsMycExpressionMutationPromoterMESVAvailable sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTF
Plasmid#162784PurposeProtein expression in CHO cells - C-terminal FLAG and 10xhis tags are codedDepositorTypeEmpty backboneUseTags10xHis and FLAGExpressionMammalianMutationPromoterCHO Elongation factor 1AAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2-zfCSF3b
Plasmid#168202PurposeExpress zebrafish Csf3b, mRNA synthesisDepositorInsertCsf3b (csf3b Zebrafish)
UseVertebrate expression (zebrafish, chicken, xenopu…TagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2-zfCSF3a
Plasmid#168201PurposeExpress zebrafish Csf3a, mRNA synthesisDepositorInsertCsf3a (csf3a Zebrafish)
UseVertebrate expression (zebrafish, chicken, xenopu…TagsExpressionMammalianMutationDeletion of A27PromoterAvailable sinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCSN071
Plasmid#166729PurposeSingle copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to Mxi1 and NLS, codon optimized for Saccharomyces cerevisiaeDepositorInsertdCas12a-Mxi1-NLS
UseCRISPRTagsMxi1 and SV40 NLSExpressionYeastMutationE925APromoterS. cerevisiae TEF1Available sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCSN072
Plasmid#166730PurposeSingle copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS, Mxi1 and NLS, codon optimized for Saccharomyces cerevisiaeDepositorInsertdCas12a-NLS-Mxi1-NLS
UseCRISPRTagsMxi1 and SV40 NLSExpressionYeastMutationE925APromoterS. cerevisiae TEF1Available sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046774
Plasmid#145574PurposeBacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymeraseDepositorInsertSARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;FLAG_2ExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046846
Plasmid#145575PurposeBacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymeraseDepositorInsertSARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;HAExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pJS263
Plasmid#134141PurposeBacterial expression of C. elegans Ska1 microtubule binding domain (MTBD)DepositorInsertSka1 MTBD-His (Y106G6H.15 Nematode)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIW087
Plasmid#134128PurposeBacterial expression of GFP-Ska1 and Ska2DepositorInsertHis-GFP-human Ska1 ; human Ska2
UseTagsGFPExpressionBacterialMutationK183A K184APromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIW084
Plasmid#134130PurposeBacterial expression of GFP-Ska1 and Ska2DepositorInsertHis-GFP-human Ska1 ; human Ska2
UseTagsGFPExpressionBacterialMutationR236APromoterAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIW095
Plasmid#134131PurposeBacterial expression of GFP-Ska1 and Ska2DepositorInsertHis-GFP-human Ska1 ; human Ska2
UseTagsGFPExpressionBacterialMutationR155APromoterAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIW090
Plasmid#134129PurposeBacterial expression of GFP-Ska1 and Ska2DepositorInsertHis-GFP-human Ska1 ; human Ska2
UseTagsGFPExpressionBacterialMutationK223A K226APromoterAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIW093
Plasmid#134137PurposeRetroviral expression of GFP-Ska1DepositorInsertGFP-human Ska1 CRISPR/Cas9 hardened (SKA1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationCRISPR resistant, R155APromoterAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIW082
Plasmid#134136PurposeRetroviral expression of GFP-Ska1DepositorInsertGFP-human Ska1 CRISPR/Cas9 hardened (SKA1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationCRISPR resistant, R236APromoterAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIW088
Plasmid#134135PurposeRetroviral expression of GFP-Ska1DepositorInsertGFP-human Ska1 CRISPR/Cas9 hardened (SKA1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationCRISPR resistant, K223A K226APromoterAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIW085
Plasmid#134134PurposeRetroviral expression of GFP-Ska1DepositorInsertGFP-human Ska1 CRISPR/Cas9 hardened (SKA1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationCRISPR resistant, K183A K184APromoterAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
Thy1.1-muSOX D219A
Plasmid#131704Purposeencodes Thy1.1 followed by a P2A site and muSOX D219A catalytic mutant for enriching pure populations of transfected cellsDepositorInsertThy1.1-muSOX
UseTagsExpressionMammalianMutationAspartic acid 219 mutated to an alaninePromoterCMVAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0.007
Plasmid#119671PurposeLevel 0 part. Promoter, cpc560 promoter, extended spacer region, downstream TFB site region duplicated.DepositorInsertPcpc560-Dx2
UseSynthetic BiologyTagsExpressionMutationbp 89 T to C, bp 187 C to A, Duplicated downstrea…PromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0.006
Plasmid#119670PurposeLevel 0 part. Promoter, cpc560 promoter, extended spacer region, upstream TFB site region duplicated.DepositorInsertPcpc560-Ux2
UseSynthetic BiologyTagsExpressionMutationbp 89 T to C, bp 187 C to A, Duplicated upstream …PromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-puro-eGFP-HEC1-d80
Plasmid#114025PurposeInducible expression of eGFP-tagged HEC1-d80 in FLPin Trex cell linesDepositorInserteGFP-d80-HEC1 (NDC80) (NDC80 Human)
UseTagsExpressionMammalianMutationHec1 - 1 to 80 deletedPromoterAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-hygro-eGFP-HEC1-d80
Plasmid#114030PurposeInducible expression of eGFP-tagged HEC1-d80 in FLPin Trex cell linesDepositorInserteGFP-d80-HEC1 (NDC80) (NDC80 Human)
UseTagsExpressionMammalianMutationHec1 - 1 to 80 deletedPromoterAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-hygro-eGFP-HEC1-8A/S15D
Plasmid#114031PurposeInducible expression of eGFP-tagged HEC1-8A/S15D in FLPin Trex cell linesDepositorInserteGFP-HEC1-8A/S15D (NDC80) (NDC80 Human)
UseTagsExpressionMammalianMutationHec1 - 8A/S15DPromoterAvailable sinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmCherry_C1 I3
Plasmid#61398PurposeExpresses mCherry-tagged Inhibitor-3 in mammalian cellsDepositorInsertInhibitor-3 (PPP1R11 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-GFP-I3
Plasmid#61401PurposeExpresses GFP-tagged Inhibitor-3 in mammalian cellsDepositorInsertInhibitor-3 (PPP1R11 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pT7 cyclin A1-164 venus cks1 (2196)
Plasmid#61839Purposeexpression of N terminal cyclin A and cks1DepositorInsertcyclin A (CCNA2 Human)
UseIn vitro transcriptionTagsVenusExpressionMutationL2VPromoterT7Available sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-puro-mMCL1 Delta C22
Plasmid#45820DepositorInsertmMCL-1 Delta C22
UseTagsExpressionMammalianMutationC-terminal 22 amino acids are deleted from full l…PromoterAvailable sinceJuly 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-puro-Delta N67-mMCL1
Plasmid#45819DepositorInsertDelta N67-mMCL-1
UseTagsExpressionMammalianMutationN-terminus 67 amino acids deleted from full lengt…PromoterAvailable sinceJuly 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBABE-miR-28
Plasmid#35686DepositorInserthsa-miR-28 (MIR28 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
L2125-FRB-GPA-NLuc-CyOFP1
Plasmid#222885PurposePositive control receptor chain for BRET signal. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, full Nanoluciferase, 10 GS linker, CyOFP1.DepositorInsertFRB-GPA-20GS-NanoLuc-10GS-CyOFP1
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7-7 asyn WT
Plasmid#36046DepositorInsertalpha-synuclein (SNCA Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141-ZmUbi-RZ-Lb
Plasmid#86197PurposeLbCpf1 Gateway gRNA entry plasmid using Zea mays Ubi promoter and ribozyme processingDepositorInsertgRNA cloning site
UseCRISPRTagsExpressionMutationPromoterMaize ubiquitin 1Available sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Tet-coBxb1-2A-BFP_IRES-iCasp9-2A-Blast_rtTA3
Plasmid#171588PurposeLentivector encoding a DNA recombinase landing pad with Bxb1 integrase and iCasp9 negative selection behind a Tet-inducible promoterDepositorInsertsBxb1 integrase
iCasp9
BFP
rtTA3G
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
CAG_IGF1R_EGFP
Plasmid#199628PurposeFRET donor for IGF1R sensorDepositorInsertIGF1R (IGF1R Human)
UseTagsEGFPExpressionMammalianMutationPromoterCAGAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJRH-1345 U6-B2M sgRNA Gag-Cas9 v2
Plasmid#201916PurposeCas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)DepositorInsertsGag-Cas9 v2
B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
UseTagsExpressionMammalianMutationPromoterCAG and Human U6Available sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
TUBB5-Halo
Plasmid#64691PurposeExpresses beta-tubulin labeled with Halo-tagDepositorInsertTUBB
UseTagsHalo-tagExpressionMammalianMutationPromoterAvailable sinceJune 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Hyg-mEGFP
Plasmid#191847PurposeExpresses the monomeric form of EGFPDepositorInsertmEGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV9retro
Plasmid#224687PurposeAAV packaging plasmid expressing Rep/Cap genesDepositorInsertRep2/Cap9retro
UseAAVTagsExpressionMutationinsertion of 10-mer peptide from AAV2retro (LADQD…PromoterAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-MKLP1
Plasmid#70145Purposemammalian expression of MKLP1 with an eGFP fusionDepositorInsertMKLP1 (KIF23 Human)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
CAHS2 (pBS0249)
Plasmid#185139PurposeFor the mammalian expression of the tardigrade protein CAHS2. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).DepositorInsertCAHS2
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hChATp-tdTomato
Plasmid#197564PurposeExpresses tdTomato under a human choline acetyltransferase (hChAT) promoter fragmentDepositorInserttdTomato
UseAAVTagsExpressionMutationPromoterhuman ChAT promoterAvailable sinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
PGK-H2BmCherry
Plasmid#21217DepositorInsertPhosphoglycerate kinase promoter (PGK1 Human)
UseLentiviralTagsH2B-mCherryExpressionMutationPromoterAvailable sinceJuly 1, 2009AvailabilityAcademic Institutions and Nonprofits only