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pcDNA5-FRT-TO-C-8His-Flag

Plasmid

#157678

Purpose

C-terminal tagging vector

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

8His-Flag

Expression

Mammalian

Available Since

Aug. 4, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pCFJ1149 - intron 3 smu-2 PATCs

Plasmid

#161516

Purpose

PATC-rich intron number three from smu-2 to stimulate germline expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron 3 from smu-2

Expression

Worm

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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p CIneo-RL-Let7-3xBulgeB

Plasmid

#115368

Purpose

Expression vector to produce humanized Renilla luciferase with three partially complementary binding sites for human let-7 miRNA in the 3'UTR

Depositor

Witold Filipowicz, Ramesh Pillai

Article

Pillai et al Science. 2005 Sep 2. 309(5740):1573-6.

Insert

Renilla Luciferase

Use

Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCFJ2359 - intron GG2 - 250bp

Plasmid

#159878

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

intron GG2 - 250bp

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pAC1750

Plasmid

#127984

Purpose

AMA1 plasmid with Aspergillus optimized Cpf1, ble selection marker, and USER cassette (PacI/Nt.BbvCI)

Depositor

Uffe Mortensen

Article

Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

Insert

Cpf1

Use

CRISPR; Fungal expression

Tags

SV40 NLS

Expression

Bacterial

Mutation

codon optimized for Aspergillus nidulans

Promoter

Aspergillus nidulans tef1 promoter

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pGL4-Gateway-SCP1

Plasmid

#71510

Purpose

humanSTARR-seq validation vector with Gateway cassette upstream of the firefly luciferase gene and the SCP1 core promoter

Depositor

Alexander Stark

Article

Arnold et al Science. 2013 Mar 1;339(6123):1074-7. do

Type

Empty backbone

Use

Humanstarr-seq validation vector

Tags

luciferase

Expression

Mammalian

Available Since

Jan. 11, 2016

Availability

Academic Institutions and Nonprofits only
HA-EcPPK1

Plasmid

#159279

Purpose

Expresses a HA-tagged E. coli PPK1 in mammalian cell lines

Depositor

Michael Downey

Article

Bondy-Chorney et al Cell Rep. 2020 Oct 27;33(4):108318. doi:

Insert

HA-tagged Polyphosphate Kinase 1

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-Chronos-mT-Sapphire

Plasmid

#149690

Purpose

For packaging and expression of Chronos-mT-Sapphire in neurons via AAV

Depositor

Robert Malenka

Article

Christoffel et al Proc Natl Acad Sci U S A. 2021 Jun 15;11

Insert

Chronos

Use

AAV

Tags

mT-Sapphire

Expression

Mammalian

Promoter

hSyn

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLN599 (pFuGW-hUbCp-hSTE1)

Plasmid

#105206

Purpose

ubiquitin C promoter drives STE expression

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

hUbCp-hSTE1

Use

Lentiviral and Synthetic Biology

Tags

HA tag

Expression

Mammalian

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV5-Flag-mEPLIN beta

Plasmid

#40956

Depositor

Elizabeth Luna, Scott Maul

Article

Maul et al Gene. 2001 Jan 10;262(1-2):155-60.

Insert

EPLIN beta (Lima1 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only
pMX231

Plasmid

#23003

Depositor

Linda Wordeman

Article

Stumpff et al Dev Cell. 2008 Feb . 14(2):252-62.

Insert

Kif18A-mut (KIF18A Human)

Tags

6His and EGFP

Expression

Mammalian

Mutation

H304A R308A K311A

Available Since

Feb. 8, 2010

Availability

Academic Institutions and Nonprofits only

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mCerulean3-PTK2-C-10

Plasmid

#55441

Purpose

Localization: FAK/Focal Adhesions, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

PTK2 (PTK2 Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only
p CIneo-RL-Let7-perf

Plasmid

#115367

Purpose

Expression vector to produce humanized Renilla luciferase with a single perfectly complementary binding site for human let-7 miRNA in the 3'UTR

Depositor

Witold Filipowicz, Ramesh Pillai

Article

Pillai et al Science. 2005 Sep 2. 309(5740):1573-6.

Insert

Renilla Luciferase

Use

Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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rTetR-GFP-VPR

Plasmid

#183918

Purpose

VPR activation domain coupled to reverse Tet repressor; labeled with GFP; binds tetO sites upon doxycycline addition

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

rTetR-GFP-VPR

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pICH47732:PtFCP:ShBle

Plasmid

#104893

Purpose

Expresses ShBle with FCP promoter/terminator, Golden Gate Assembly, for Phaeodactylum transformation.

Depositor

Thomas Mock

Article

Graff van Creveld et al Front Microbiol. 2021 Sep 8;12:688199. d

Insert

ShBle

Use

Phaeodactylum; golden gate assembly

Tags

PtFCP promoter and terminator

Available Since

Dec. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pTriEx4-H6-FGB

Plasmid

#49203

Purpose

Fluorescent reporter for cGMP

Depositor

John Garthwaite

Article

Bhargava et al Front Mol Neurosci. 2013 Sep 24;6:26. do

Insert

H6-FGB

Tags

His tag + Other tags

Expression

Bacterial, Insect, and Mamm…

Mutation

None

Promoter

CMV

Available Since

Dec. 4, 2013

Availability

Academic Institutions and Nonprofits only

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LbCas12a

Plasmid

#188493

Purpose

Expresses FLAG tagged LbCas12a

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

Cas12a

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_IDH1

Plasmid

#183300

Purpose

All-in-One CRISPRko system with a guide RNA that targets IDH1 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

IDH1

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
mTagRFP-T-Vinculin-23

Plasmid

#58030

Purpose

Localization: Focal Adhesions, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Vinculin (VCL Human)

Tags

mTagRFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 4, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pREF-GFP kan

Plasmid

#122061

Purpose

reporter plasmid for termination and terminator override to express GFP. Marked with URA3

Depositor

Daniel Reines

Article

Reines et al Methods. 2019 Jan 4. pii: S1046-2023(18)

Inserts

intergenic terminator from yeast IMD2
green fluorescent protein

Expression

Yeast

Promoter

GAL1 and GAL1 comes in pYES2

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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MXS_PGK::rtTA3-bGHpA

Plasmid

#62446

Purpose

MXS_chaining vector with PGK::rtTA3-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

cassette containing the reverse tetracycline transactivator with PGK promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCB-CBEv1

Plasmid

#221138

Purpose

Coxiella burnetii CRISPR-Cas9 cytosine base editing plasmid version 1 without sgRNA construct. Expresses 3xF-HF-BE3.

Depositor

Craig Roy

Article

Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

Insert

3xF-rAPOBEC1-Cas9n(HF)-UGI (HF-BE3)

Use

CRISPR

Tags

3xFLAG

Expression

Bacterial

Promoter

lacIq-Ptac

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSicoR-mCh-Oct4i

Plasmid

#21906

Depositor

Miguel Ramalho-Santos

Article

Gaspar-Maia et al Nature. 2009 Aug 13;460(7257):863-8. doi

Insert

Oct4i

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 30, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
CROP_C9_Puro_IDH2

Plasmid

#183301

Purpose

All-in-One CRISPRko system with a guide RNA that targets IDH2 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

IDH2

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pPI278

Plasmid

#113227

Purpose

extrachromosomal expression vector, expression of H2B-GFP

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Insert

H2B

Use

Dictyostelium discoideum expression vector, extra…

Tags

GFP

Promoter

act15

Available Since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5-hygro-eGFP-HEC1-9A

Plasmid

#114028

Purpose

Inducible expression of eGFP-tagged HEC1-9A in FLPin Trex cell lines

Depositor

Geert Kops

Article

Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

Insert

eGFP-HEC1 - 9A (NDC80) (NDC80 Human)

Expression

Mammalian

Mutation

Hec1 - 9A

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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MXS_PGK::HygroR-bGHpA

Plasmid

#62436

Purpose

MXS_chaining vector with PGK::HygroR-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

resistance cassette against hygromycin B with PGK Promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pLD-puro-CnVA

Plasmid

#24587

Depositor

Jason Moffat

Article

Mak et al Mol Cell Proteomics. 2010 May;9(5):811-2

Insert

Gateway(TM) cassette

Use

Lentiviral

Tags

VA

Expression

Mammalian

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only
pEN_TTRmiRc2

Plasmid

#25754

Purpose

Entry vector for cloning miR30-based shRNA driven by TRE-tight promoter with dsRed2 coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Entry vector

Tags

dsRed2

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pEN_TGmiRc3

Plasmid

#25749

Purpose

Entry vector for cloning miR30-based shRNA driven by TRE promoter with GFP coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Entry vector

Tags

GFP

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pCFJ2370 - intron GG3 - 250bp

Plasmid

#159879

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

intron GG3 - 250bp

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
IBMc460

Plasmid

#161940

Purpose

Carries Golden Gate substitution insert for IBM with gp41-1 intein. PPhlF as C-lobe promoter

Depositor

Baojun Wang

Article

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pSB1A3-BbsI-GY-gp-41-1N-cat-PPhlF-RiboJ-B32-gp41-1C-SS-SapI

Expression

Bacterial

Promoter

PPhlF

Available Since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
p4456 FLAG-hPLIC-2 NTF

Plasmid

#8662

Depositor

Peter Howley

Article

Kleijnen et al Mol Cell 2000 Aug;6(2):409-19.

Insert

hPLIC-2 (UBQLN2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

N-terminal fragment: amino acids 1-107 of hPLIC-2

Available Since

June 16, 2005

Availability

Academic Institutions and Nonprofits only
rtTA and TagBFP cassettes

Plasmid

#62452

Purpose

MXS_chaining vector with 2 cassettes

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Inserts

CMV::NLS-TagBFPx3-bGHpA
CMV::rtTA3-bGHpA

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pLEX_970_puro_DEST_V5_BFP

Plasmid

#45297

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

BFP

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
pAAV-myrSNAP-CON

Plasmid

#60099

Purpose

Expresses membrane-targeted SNAP(f)-tag and histone-GFP, conditional

Depositor

Gregory Jefferis

Article

Kohl et al Proc Natl Acad Sci U S A. 2014 Aug 25. p

Inserts

SNAP-tag
histone-GFP

Use

AAV and Mouse Targeting

Expression

Mammalian

Available Since

March 26, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
MXS_CMV::HygroR-bGHpA

Plasmid

#62435

Purpose

MXS_chaining vector with CMV::HygroR-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

resistance cassette against hygromycin B with CMV Promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pT3TS-nCas9n-A71

Plasmid

#125644

Purpose

micro-injection of poly-adenylated nCas9n RNA

Depositor

Jason Berman

Article

Prykhozhij et al Methods Mol Biol. 2020;2115:385-405. doi

Insert

nCas9n

Use

Rna in vitro transcription vector

Promoter

T3

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pFIN-NRLL-tdTOM

Plasmid

#44355

Depositor

Susan Semple-Rowland

Article

Semple-Rowland et al Mol Vis. 2010 May 27;16:916-34.

Insert

NRLL-tdTOM

Use

Lentiviral

Promoter

mouse neural leucine zipper (pos 3127-5587)

Available Since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
JAB2296

Plasmid

#190365

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_pro35S-PhlFO::GFPint-NLS-ADHt)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proUBQ10::PIP2A-mCherry-NOSt_pro35S-PhlFO::GFPint-NLS-ADHt

Expression

Plant

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
CROP_C9_Puro_MYC

Plasmid

#183330

Purpose

All-in-One CRISPRko system with a guide RNA that targets MYC gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

MYC

Use

Lentiviral

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
MXS_CMV::CreERT2-bGHpA

Plasmid

#62443

Purpose

MXS_chaining vector with CMV::CreERT2-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

cassette containing the tamoxifen inducible Cre recombinase with CMV Promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N1-8x

Plasmid

#172284

Purpose

Expressing EGFP mRNA fused with 8 tandem repeats of a 50-base sequence

Depositor

Antony K Chen

Article

Chen et al Sci Rep. 2017 May 8;7(1):1550. doi: 10.1

Insert

EGFP-N1-8x

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-FBL6 delta6

Plasmid

#19925

Depositor

Yue Xiong

Article

Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

Insert

FBL6 delta 6 (FBXL6 Human)

Expression

Mammalian

Mutation

delta 6

Available Since

May 7, 2009

Availability

Academic Institutions and Nonprofits only
Mouse NCKX3 (pcDNA3.1+)

Plasmid

#75204

Purpose

Mammalian expression of SLC24A3

Depositor

Jonathan Lytton

Article

Kraev et al J Biol Chem. 2001 Jun 22;276(25):23161-7

Insert

SLC24A3 (Slc24a3 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pBigTB-FlpERT2

Plasmid

#149435

Purpose

tamoxifen-inducible Flp recombinase

Depositor

Primo Schaer

Article

Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.126

Insert

Flp-ERT2 fusion protein

Use

Cre/Lox and Mouse Targeting

Tags

ERT2

Expression

Mammalian

Promoter

CAG promoter

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRL_yEGFP_hc

Plasmid

#98746

Purpose

Reporter plasmid for the PhiReX system. Encodes the reporter gene yEGFP under the control of the CYC1 minimal promoter with upstream synTALE-DBS.

Depositor

Bernd Müller-Röber

Article

Hochrein et al Nucleic Acids Res. 2017 Sep 6;45(15):919

Insert

Yeast-enhanced green fluorescent protein

Use

Synthetic Biology

Expression

Yeast

Promoter

synthetic

Available Since

Aug. 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDYSCKO-ADE1

Plasmid

#120264

Purpose

pDYSCKO canavanine co-selection vector with a guide targeting the ADE1 locus

Depositor

Christian Landry

Article

Despres et al G3 (Bethesda). 2018 Oct 3;8(10):3163-317

Insert

pDYSCKO cassette-ADE1 targeting guide

Use

CRISPR and Synthetic Biology

Promoter

SNR52

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MXS_PGK::tTA2-bGHpA

Plasmid

#62448

Purpose

MXS_chaining vector with PGK::tTA2-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

cassette containing the tetracycline transactivator with PGK promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
CROP_C9_Puro_FLT4

Plasmid

#183294

Purpose

All-in-One CRISPRko system with a guide RNA that targets FLT4 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

FLT4

Use

Lentiviral

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 13,355 results for: ache