We narrowed to 8,905 results for: Ott

Showing: 3321 - 3360 of 8905 results

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pET21d-myc-BirA

Plasmid

#109424

Purpose

Bacterial expression of biotin ligase (BirA) with an N-terminal myc epitope

Depositor

Martin Spiess

Article

Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

Insert

BirA (birA )

Tags

myc

Expression

Bacterial

Promoter

T7

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-TVAmCherry-2A-oG

Plasmid

#104330

Purpose

AAV constitutively expressing TVA-mCherry fusion and optimised Rabies Glycoprotein (oG)

Depositor

Marco Tripodi

Article

Ciabatti et al Cell. 2017 Jul 1. pii: S0092-8674(17)306

Inserts

TVA-mCherry fusion protein
Optimised Rabies Glycoprotein (oG)

Use

AAV; Adeno associated viral vector

Available Since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.iGABASnFR

Plasmid

#112172

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR

Use

AAV

Promoter

GFAP

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pRRL MND GFP

Plasmid

#36247

Depositor

David Rawlings

Article

Astrakhan et al Blood. 2012 Mar 19.

Insert

GFP (eGFP )

Use

Lentiviral

Promoter

MND

Available Since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

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pPAmCherry-b-actin

Plasmid

#31949

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

Insert

PAmCherry1

Tags

b-actin

Expression

Mammalian

Mutation

E26V/A58T/K69N/L84F/N99K/S148L/I165V/Q167P/L169V/…

Promoter

CMV

Available Since

Sept. 22, 2011

Availability

Academic Institutions and Nonprofits only

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pRSF-G1(6/7FTrp)RS

Plasmid

#207621

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of 6-Fluoro-L-Tryptophan (6FTrp) and 7-Fluoro-L-Tryptophan (7FTrp) into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Qianzhu et al J Am Chem Soc. 2024 Apr 30. doi: 10.1021

Insert

tRNA synthetase

Expression

Bacterial

Promoter

T7

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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AAV2_HLF-ZE

Plasmid

#175034

Purpose

HLF targeting construct introducing an ires ZsG P2A tEGFR cassette downstream of the human HLF stop codon

Depositor

Guy Sauvageau

Article

Lehnertz et al Blood. 2021 Sep 9. pii: S0006-4971(21)01

Insert

HLF ires ZsGreen P2A tEGFR

Use

AAV

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pTwist GFP-Linker-VDAC1

Plasmid

#211735

Purpose

Expresses GFP tagged VDAC1 in mammalian cells. GFP is attached to the N-terminus of VDAC1 with a flexible linker.

Depositor

Roland Malli

Article

Pilic et al PLoS One. 2024 Oct 18;19(10):e0311107. d

Insert

Voltage-dependent anion channel 1 (VDAC1 )

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

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GCaMP-AMPK-SPARK

Plasmid

#226257

Purpose

Dual reporter of Ca2+ levels and AMPK activity

Depositor

Roland Malli

Article

Erdogan et al ACS Sens. 2024 Aug 21. doi: 10.1021/acss

Insert

GCaMP-AMPK-SPARK

Expression

Mammalian

Available Since

Oct. 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pQE-60 roGFP2-Orp1-His

Plasmid

#64976

Purpose

Bacterial expression of His-tagged roGFP2-Orp1

Depositor

Tobias Dick

Article

Gutscher et al J Biol Chem. 2009 Nov 13;284(46):31532-4

Insert

Orp1 (HYR1 Budding Yeast)

Tags

His and roGFP2

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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CS2 Flag-Smad2

Plasmid

#14042

Depositor

Joan Massague

Article

Hata et al Nature. 1997 Jul 3. 388(6637):82-7.

Insert

Smad2 (SMAD2 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Available Since

May 10, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Ii-OVA.II

Plasmid

#64597

Purpose

Expression of invariant chain fused to chicken ovalbumin 315-353 amino acids as model antigen for study of MHC class II presentation

Depositor

Sandra Diebold, Martin Zenke

Article

Diebold et al Gene Ther. 2001 Mar;8(6):487-93.

Insert

Ovalbumin

Tags

Invariant chain

Expression

Mammalian

Mutation

Only amino acids 315-353 (class II epitopes)

Promoter

CMV

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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HisTEV-cDAXX

Plasmid

#197012

Purpose

E. coli expression vector

Depositor

Tanja Mittag

Article

Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

Insert

DAXX

Tags

6His-TEV

Expression

Bacterial

Available Since

April 18, 2023

Availability

Academic Institutions and Nonprofits only

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pmScarlet_NES_C1

Plasmid

#85060

Purpose

In vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

NES

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pMTS-HA-GFP11

Plasmid

#177725

Purpose

Eleventh β-strand of GFP (GFP11) fused to a mitochondria signal sequence for mitochondrial targeting, HA tagged

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

MTS-HA-GFP11

Tags

GFP11 and HA

Expression

Bacterial and Mammalian

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pET-MCN-10His-StayGold

Plasmid

#211360

Purpose

Bacterial expression of the bright and photostable StayGold fluorescent protein. Codon optimised for expression in Escherichia coli. Contains a N-terminal 10xHis tag.

Depositor

Mohan Balasubramanian

Article

Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

Insert

StayGold

Tags

10xHis-tag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pPP381

Plasmid

#158798

Purpose

pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-2(D394A) in MCS1 for expression in E. coli and protein purification

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

dCasPhi-2

Tags

6xHis

Expression

Bacterial

Mutation

RuvC-inactivating mutation D394A

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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VinculinTS-A50I/I997A

Plasmid

#111829

Purpose

Vinculin tension sensor with point mutations affecting ability to bind talin and actin

Depositor

Brenton Hoffman

Article

Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

Insert

VinculinTS (I997A)

Expression

Mammalian

Mutation

changed vinculin alanine 50 to isoleucine and iso…

Promoter

CMV

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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Dsup pLVX-Puro

Plasmid

#180635

Purpose

Expresses human codon optimized tardigrade damage suppressing protein Dsup under a CMV promoter; to provide increased radio tolerance

Depositor

Christopher Mason

Article

Westover et al bioRxiv 2020.11.10.373571

Insert

Dsup

Use

Lentiviral

Promoter

CMV

Available Since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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dCas9-CRY2Clust

Plasmid

#200969

Purpose

induction of phase-separated condensate by CRY2Clust

Depositor

Jian Xu

Article

Kim et al Sci Adv. 2023 Mar 31;9(13):eadg1123. doi

Insert

CRY2Clust

Use

Lentiviral

Expression

Bacterial and Mammalian

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRSF-G1(4/5FTrp)RS

Plasmid

#207620

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of 4-Fluoro-L-Tryptophan (4FTrp) and 5-Fluoro-L-Tryptophan (5FTrp) into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Qianzhu et al J Am Chem Soc. 2024 Apr 30. doi: 10.1021

Insert

tRNA synthetase

Expression

Bacterial

Promoter

T7

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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pPP537

Plasmid

#158803

Purpose

pRSF-Duet1 derived plasmid containing C-terminally 2xSV40 NLS and hexa-histidine tagged CasΦ-2 in MCS1 for expression in E. coli and protein purification

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

CasPhi-2

Tags

2xSV40NLS-6xHis

Expression

Bacterial

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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AAV2_HLF-ZP

Plasmid

#175035

Purpose

HLF targeting construct introducing an ires ZsG P2A Puro cassette downstream of the human HLF stop codon

Depositor

Guy Sauvageau

Article

Lehnertz et al Blood. 2021 Sep 9. pii: S0006-4971(21)01

Insert

HLF ires ZsGreen P2A Puro

Use

AAV

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pET22-HT/plsA

Plasmid

#73625

Purpose

Produces Escherichia coli adenylate kinase (PlsA), N-terminal His6 tag

Depositor

T. J. Kappock, James Williamson

Article

Schultheisz et al ACS Chem Biol. 2008 Aug 15;3(8):499-511.

Insert

adenylate kinase (adk Escherichia coli str. K-12 substr. MG1655)

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pGEmCherry2

Plasmid

#118413

Purpose

Red fluorescent reporter for assessing CRISPR gRNA activity

Depositor

Mark Denham

Article

Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

Insert

mCherry

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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FUGW-VGLUT2-pHluorin

Plasmid

#207469

Purpose

Lentiviral expression of VGLUT2-pHluorin

Depositor

Robert Edwards

Article

Silm et al Neuron. 2019 May 22;102(4):786-800.e5. d

Insert

VGLUT2 (Slc17a6 Rat)

Use

Lentiviral

Tags

pHluorin

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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FSW-HRP-TM

Plasmid

#82540

Purpose

Lentiviral vector for expressing HRP-TM in neurons

Depositor

Alice Ting

Article

Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

Insert

HRP fused to TM domain of PDGFR

Use

Lentiviral

Tags

HRP

Expression

Mammalian

Promoter

Human Synapsin

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pMTS-hFluc

Plasmid

#177716

Purpose

Firefly luciferase reporter fused to a mitochondria signal sequence for mitochondrial targeting

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

hFluc

Tags

Mitochondrial Targeting Sequence MTS SS

Expression

Bacterial, Mammalian, and Y…

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD/HisB-PAmCherry1

Plasmid

#31931

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

Insert

PAmCherry1

Tags

His

Expression

Bacterial

Mutation

E26V/A58T/K69N/L84F/N99K/S148L/I165V/Q167P/L169V/…

Promoter

araB

Available Since

Sept. 7, 2011

Availability

Academic Institutions and Nonprofits only

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pET28a-mH6-Cas12i1

Plasmid

#120882

Purpose

Expresses E. coli codon optimized Cas12i1 effector in the pET28a backbone.

Depositor

Arbor Biotechnologies

Article

Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

Insert

Cas12i1

Use

CRISPR

Tags

mH6

Expression

Bacterial

Mutation

WT

Promoter

lac

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2-mCherry_SNAP_SNAPV_PACT_Active

Plasmid

#173072

Purpose

Active SNAP tag at the centrosome

Depositor

Heidi Hehnly

Article

Bucko et al Elife. 2019 Dec 24;8. pii: 52220. doi: 1

Insert

mCherry-SNAP-SNAP-Varied-PACT

Tags

mCherry-SNAP-SNAP-Varied

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pTfR-PAmCherry1

Plasmid

#31948

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

Insert

PAmCherry1

Tags

transferrin receptor (TfR)

Expression

Mammalian

Mutation

E26V/A58T/K69N/L84F/N99K/S148L/I165V/Q167P/L169V/…

Promoter

CMV

Available Since

Sept. 22, 2011

Availability

Academic Institutions and Nonprofits only

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JC201: pMVP/Ad/5-35-DEST

Plasmid

#121847

Purpose

pMVP destination vector, empty 5/35 fiber-modified adenovirus vector backbone

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Adenoviral; Pmvp gateway destination vector

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pFS478

Plasmid

#89066

Purpose

KanMX Z3EV promoter cassette

Depositor

Nick Rhind

Article

Ohira et al Yeast. 2017 Aug;34(8):323-334. doi: 10.1

Insert

Z3EV promoter cassette

Use

Yeast integration vector

Mutation

yeast Gal1 promoter modified with multiple Zif268…

Promoter

Z3EV promoter

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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mCherry-Vinculin-N-21

Plasmid

#55160

Purpose

Localization: Focal Adhesions, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Article

Burnette et al Nat Cell Biol. 2011 Apr;13(4):371-81. do

Insert

Vinculin (VCL Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only
pPAmCherry3-C1

Plasmid

#31933

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

Insert

PAmCherry3

Expression

Mammalian

Mutation

M18L/A58T/K69N/L84F/M137I/E146D/S148L/I165L/Q167A…

Promoter

CMV

Available Since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-DeltaJunD

Plasmid

#68547

Purpose

AAV expression of DeltaJunD

Depositor

Eric Nestler

Article

Winstanley et al J Neurosci. 2007 Sep 26;27(39):10497-507

Insert

DeltaJunD-IRES-GFP

Use

AAV

Expression

Mammalian

Mutation

truncated splice variant

Promoter

CMV

Available Since

July 11, 2018

Availability

Academic Institutions and Nonprofits only
pAAV.hSyn-FLEX.iGABASnFR

Plasmid

#112163

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Has Service

AAV1

Insert

iGABASnFR

Use

AAV

Promoter

Synapsin

Available Since

July 16, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAcGFP1-N1-CAHS3

Plasmid

#90031

Purpose

To express GFP-tagged CAHS3 in mammalian cells transiently

Depositor

Takekazu Kunieda

Article

Yamaguchi et al PLoS One. 2012;7(8):e44209.

Insert

CAHS3

Tags

AcGFP1

Expression

Mammalian

Promoter

CMV

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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SauCas9 EMX1-pegRNA

Plasmid

#169857

Purpose

SauCas9 pegRNA for EMX1

Depositor

Scot Wolfe

Article

Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

Insert

SauCas9 EMX1-pegRNA

Expression

Mammalian

Promoter

human U6 promoter

Available Since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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We narrowed to 8,905 results for: Ott