8,727 results

1. pET28a_T7-ARG1

   Plasmid

   #106473

   Purpose

   Expresses acoustic reporter genes or gas vesicles (ARG1) in Escherichia coli

   Depositor

   Mikhail Shapiro

   Article

   Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

   Insert

   ARG1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. BFP-KDEL

   Plasmid

   #49150

   Purpose

   visualization of the ER

   Depositor

   Gia Voeltz

   Article

   Friedman et al Science. 2011 Oct 21;334(6054):358-62. d

   Insert

   BiP ER signal sequence (HSPA5 Human)
   

   Use

   Tags

   BFP and KDEL

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-Egr1

   Plasmid

   #11729

   Depositor

   Eileen Adamson

   Article

   Yu et al Mol Cell. 2004 Jul 2. 15(1):83-94.

   Insert

   Egr-1 (Egr1 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. EF.CMV.RFP

   Plasmid

   #17619

   Depositor

   Linzhao Cheng

   Article

   Yu et al Mol Ther. 2003 Jun . 7(6):827-38.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 27, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYLsgRNA-OsU6b

   Plasmid

   #66196

   Purpose

   cloning of sgRNAs for expression in plants

   Depositor

   Yao-Guang Liu

   Article

   Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

   Type

   Empty backbone

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   OsU6b

   Available since

   Aug. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. lenti sgRNA(MS2)_zeo backbone

   Plasmid

   #61427

   Purpose

   3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Feng Zhang

   Article

   Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and EF1A

   Available since

   Dec. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLJM1-FIRE-pHLy

   Plasmid

   #170775

   Purpose

   Ratiometric biosensor expressed with CMV promoter for probing lysosomal pH in mammalian cells

   Depositor

   Aimee Kao

   Article

   Chin et al ACS Sens. 2021 Jun 8. doi: 10.1021/acsse

   Insert

   LAMP1 (LAMP1 Human)
   

   Use

   Lentiviral

   Tags

   mCherry and mTFP1

   Expression

   Mammalian

   Mutation

   84 nucleotide Signal Sequence switched to the beg…

   Promoter

   CMV

   Available since

   Sept. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV.hSyn.iGluSnFR3.v857.SGZ

   Plasmid

   #178330

   Purpose

   Fluorescent reporter for glutamate, third generation, variant 857 in Stargazin (SGZ) backbone. iGluSnFR3.v857.SGZ

   Depositor

   Kaspar Podgorski

   Article

   Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

   Insert

   pAAV hSyn iGluSnFR3 v857.SGZ
   

   Use

   AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

   Tags

   IgK-chain, Myc epi tag, and Stargazin and PDZ dom…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. GFP-BRD4

   Plasmid

   #65378

   Purpose

   GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

   Depositor

   Kyle Miller

   Article

   Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

   Insert

   BRD4 (BRD4 Human)
   

   Use

   Tags

   EmGFP

   Expression

   Mammalian

   Mutation

   R1232W

   Promoter

   CMV

   Available since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-sadCas9-VP64

    Plasmid

    #115790

    Purpose

    An AAV vector expressing S. aureus dCas9 fused to VP64.

    Depositor

    Nadav Ahituv

    Article

    Matharu et al Science. 2018 Dec 13. pii: science.aau06

    Insert

    Sa-dCas9
    

    Use

    AAV

    Tags

    NLS and NLS-VP64

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCI-M2

    Plasmid

    #44170

    Depositor

    John Olsen

    Article

    Patel et al J Gene Med. 2013 Jan;15(1):51-62. doi: 1

    Insert

    Influenza A virus M2 matrix gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTriEx-RhoA FLARE.sc Biosensor WT

    Plasmid

    #12150

    Depositor

    Klaus Hahn

    Article

    Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

    Insert

    RhoA Biosensor (RHOA Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBabe-Kras G12D

    Plasmid

    #58902

    Purpose

    Expresses human Kras with a G12D Mutation

    Depositor

    Channing Der

    Insert

    KRAS (KRAS Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    G12D mutation

    Promoter

    Available since

    Sept. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCRIS-PITChv2-dTAG-BSD (BRD4)

    Plasmid

    #91795

    Purpose

    PITCh dTAG donor vector for FKBP_F36V-2xHA-P2A-BSD knock-in into the C-terminus of the human BRD4 locus.

    Depositor

    James Bradner, Behnam Nabet

    Article

    Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

    Insert

    FKBP_F36V-2xHA-P2A-BSD
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Phenylalanine 36 to valine in FKBP12

    Promoter

    Promotorless

    Available since

    March 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-Cas9-6xHis

    Plasmid

    #62374

    Purpose

    Expression of Cas9-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    Cas9_6xHis
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pICH41780

    Plasmid

    #48019

    Depositor

    Sylvestre Marillonnet

    Article

    Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pSAM_Ec

    Plasmid

    #102939

    Purpose

    Transposon mutagenesis and Tn-seq

    Depositor

    Matthew Mulvey

    Article

    Wiles et al PLoS Genet. 2013;9(8):e1003716. doi: 10.

    Insert

    mariner transposon flanked by MmeI modified inverted repeats and the himar1C9 transposase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-mNeptune2.5

    Plasmid

    #51310

    Purpose

    Expresses far-red fluorescent protein mNeptune2.5 in mammalian cells

    Depositor

    Michael Lin

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Insert

    mNeptune2.5
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. PB-TRE-dCas9-VPR

    Plasmid

    #63800

    Purpose

    SP-dCas9-VPR with doxycycline-inducible expression

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

    Insert

    dCas9-VPR
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    VPR (VP64-p65-Rta)

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. Integrin alpha L - mCFP

    Plasmid

    #8637

    Depositor

    Timothy Springer

    Article

    Kim et al Science 2003 Sep 19;301(5640):1720-5.

    Insert

    Integrin, alpha L (ITGAL Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge